{"id":8200,"date":"2016-12-20T11:25:14","date_gmt":"2016-12-20T03:25:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8200"},"modified":"2025-03-21T15:06:42","modified_gmt":"2025-03-21T07:06:42","slug":"%e4%b8%8d%e6%9c%bd%e5%8c%96%e4%ba%ba%e9%a1%9e%e9%bb%91%e7%b4%a0%e7%b4%b0%e8%83%9e-immortalized-human-melanocytes-htert%e8%b2%a8%e8%99%9f-t0462","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8200","title":{"rendered":"\u4e0d\u673d\u5316\u4eba\u985e\u9ed1\u7d20\u7d30\u80de | Immortalized Human Melanocytes-hTERT\u8ca8\u865f T0462"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong> |<\/strong>\u8ca8\u865f<\/strong> T0462 \u00a01×106 cells \/ 1.0 ml<\/strong>\u4e0d\u673d\u5316<\/strong>\u4eba\u985e<\/strong>\u9ed1\u7d20\u7d30\u80deImmortalized Human Melanocytes-hTERT<\/strong><\/p>\n

\u4e0d\u673d\u5316<\/strong>\u4eba\u985e<\/strong>\u9ed1\u7d20\u7d30\u80de <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T0462)<\/strong><\/p>\n

Immortalized Human Melanocytes-hTERT\u7d30\u80deAdherent \/ Polygonal | \u4e0d\u6747\u65b9\u6cd5: Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene | \u4f86\u6e90Skin (Homo sapiens) | \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) <\/span>\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)<\/span>\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowII (\u8ca8\u865fTM002<\/span>)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)<\/span>\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C.<\/p>\n

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\u7d30\u80de\u7279\u6027<\/strong>:<\/strong> 2-3 days when split 1:3<\/strong>\u3002\u578b\u614b\u70baPolygonal\u3002\u5efa\u8b70\u751f\u9577\u5bc6\u5ea6in T25 flasks is between 0.5-0.7 X 10^6.\u3002\u89e3\u51cd\u7d30\u80de\u5efa\u8b70\u7d30\u80de\u6578\u70ba106<\/span><\/sup>\u00a0( T25 flask)\u3002\u7d30\u80de\u57f9\u990a\u6db2\u66f4\u63db\u5927\u7d042-3\u5929\uff1b\u7d30\u80de\u7e7c\u4ee3\u70ba\u80f0\u86cb\u767d\u9176\u6eb6\u6db20.25% trypsin(TM050) \u7684\u4e0b37\u2103\u4e0bincubator 3-5\u5206\u9418\uff0c\u76f4\u5230\u7d30\u80de\u74b0\u7e5e\uff0c\u5728\u986f\u5fae\u93e1\u4e0b\u4e0d\u6642\u76e3\u6e2c\u3002\u7d30\u80de\u5916\u57fa\u8ceaCoating dish\uff0c\u7528PBS\u7a00\u91cbG422\uff081\uff1a1\uff09\u4e26\u5857\u8986\u66f4\u5c11\u7684\u6642\u9593\u3002\u81a0\u539f\u86cb\u767d\u542b\u91cf\u8f03\u9ad8\uff0c\u5f9e\u800c\u4f7f\u8207\u7d30\u80de\u7684\u7d50\u5408\u66f4\u5f37\u3002\u53ef\u4ee5\u5617\u8a66\u6e1b\u5c11incubator\u6642\u9593\u4ee5\u53ca\u5857\u8986\u66f4\u8584\u7684\u5c64\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u8cc7\u6599\u8acb\u6309\u9019PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a><\/p>\n

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) |\u8ca8\u865f T0462 \u00a01×10 […]<\/p>\n","protected":false},"author":3,"featured_media":8201,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453,455],"tags":[4079],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8200"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8200"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8200\/revisions"}],"predecessor-version":[{"id":50869,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8200\/revisions\/50869"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8201"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8200"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}