{"id":8006,"date":"2016-12-06T17:31:55","date_gmt":"2016-12-06T09:31:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8006"},"modified":"2022-07-22T16:59:10","modified_gmt":"2022-07-22T08:59:10","slug":"%e8%86%a0%e5%8e%9f%e8%9b%8b%e7%99%bdcollagen-type-i%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fsea571hu","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8006","title":{"rendered":"\u81a0\u539f\u86cb\u767dCollagen Type I\u6aa2\u6e2c\u8a66\u5291\u76d2 |\u8ca8\u865fSEA571Hu"},"content":{"rendered":"

(Uncoated)\u00a0<\/strong>\u81a0\u539f\u86cb\u767d<\/strong>Collagen<\/strong> Type I<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/strong>|<\/strong>\u8ca8\u865f<\/strong>SEA571Hu<\/a><\/span><\/p>\n

ELISA Kit for Collagen Type I (COL1\uff09<\/p>\n

\u5546\u54c1: (Uncoated) \u81a0\u539f\u86cb\u767dCollagen Type I\u6aa2\u6e2c\u8a66\u5291\u76d2(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/u>|\u8ca8\u865fSEA571Hu\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea662.5-4000pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea626.8pg\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002Cloud-Clone corp\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002Uncoated<\/u><\/strong> ELISA<\/u><\/strong>\u578b\u5f0f<\/u><\/strong>\uff1b\u5305\u62ecCapture Antibody, Detection Antibody\u3001Standard\u3001Streptavidin-HRP\u3001TMB Substrate, 96-well Plate\u3002<\/p>\n

\u81a0\u539f\u86cb\u767dCollagen Type I\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Pre-coated, ready to use 96-well strip plate<\/td>\n1<\/td>\nPlate sealer for 96 wells<\/td>\n4<\/td>\n<\/tr>\n
Standard<\/td>\n2<\/td>\nStandard Diluent<\/td>\n1\u00d720mL<\/td>\n<\/tr>\n
Detection Reagent A<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent A<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
Detection Reagent B<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent B<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n1\u00d79mL<\/td>\nStop Solution<\/td>\n1\u00d76mL<\/td>\n<\/tr>\n
Wash Buffer (30 \u00d7 concentrate)<\/td>\n1\u00d720mL<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u81a0\u539f\u86cb\u767dCollagen Type I\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong>\u7c21\u55ae\u6d41\u7a0b<\/strong><\/p>\n

    \n
  1. Prepare all reagents, samples and standards
    \n2. Add 100\u00b5L standard or sample to each well. Incubate 1 hours at 37\u00b0C;
    \n3. Aspirate and add 100\u00b5L prepared Detection Reagent A. Incubate 1 hour at 37\u00b0C;
    \n4. Aspirate and wash 3 times;
    \n5. Add 100\u00b5L prepared Detection Reagent B. Incubate 30 minutes at 37\u00b0C;
    \n6. Aspirate and wash 5 times;
    \n7. Add 90\u00b5L Substrate Solution. Incubate 15-25 minutes at 37\u00b0C;
    \n8. Add 50\u00b5L Stop Solution. Read at 450nm immediately.<\/li>\n<\/ol>\n

    \u81a0\u539f\u86cb\u767dCollagen Type I<\/strong>\u6aa2<\/strong>\u6e2c<\/strong>\u76f8\u95dc<\/strong>\u4e4b\u6297\u9ad4 | \u86cb\u767d\u8cea | ELISA | CLIA\u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n
    Catalog No.<\/td>\nRelated products for research use of Homo sapiens (Human) Organism species<\/td>\nApplications<\/td>\n<\/tr>\n
    NPA571Hu01<\/td>\nNative Collagen Type I (COL1)<\/strong><\/a><\/td>\nSDS-PAGE; WB; ELISA; IP.<\/td>\n<\/tr>\n
    PAA571Hu01<\/td>\nPolyclonal Antibody to Collagen Type I (COL1)<\/strong><\/a><\/td>\nWB, ICC, IHC-P, IHC-F, ELISA<\/td>\n<\/tr>\n
    LAA571Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Collagen Type I (COL1)<\/strong><\/a><\/td>\nIHC;WB;ELISA<\/td>\n<\/tr>\n
    SEA571Hu<\/td>\nELISA Kit for Collagen Type I (COL1)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    (Uncoated)\u00a0\u81a0\u539f\u86cb\u767dCollagen Type I\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u8ca8\u865fSEA571Hu ELISA K […]<\/p>\n","protected":false},"author":3,"featured_media":6748,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452],"tags":[4122],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8006"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8006"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8006\/revisions"}],"predecessor-version":[{"id":32550,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8006\/revisions\/32550"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6748"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8006"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8006"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8006"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}