{"id":8000,"date":"2016-12-06T12:39:02","date_gmt":"2016-12-06T04:39:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8000"},"modified":"2025-03-21T15:11:02","modified_gmt":"2025-03-21T07:11:02","slug":"%e4%b8%8d%e6%9c%bd%e5%a4%a7%e9%bc%a0%e5%bf%83%e8%82%8c%e7%b4%b0%e8%83%9e-sv40-immortalized-rat-cardiomyocytes%e8%b2%a8%e8%99%9f-t0015","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8000","title":{"rendered":"\u4e0d\u673d\u5927\u9f20\u5fc3\u808c\u7d30\u80de | SV40 Immortalized Rat Cardiomyocytes\u8ca8\u865f T0015"},"content":{"rendered":"

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a><\/strong>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a><\/strong>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a><\/strong>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a><\/strong>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/strong><\/span><\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong> |<\/strong>\u8ca8\u865f<\/strong> T0015 \u00a01×106<\/sup> cells \/ 1.0 ml.\u00a0<\/strong><\/p>\n

\u4e0d<\/strong>\u673d<\/strong>\u5927\u9f20<\/strong>\u5fc3\u808c<\/strong>\u7d30\u80de\u00a0 Immortalized Rat Cardiomyocytes |\u00a0<\/strong>\u5927\u9f20<\/strong>\u5fc3\u808c<\/strong>\u7d30\u80de<\/strong>\u57f9\u990a\u689d\u4ef6<\/strong>: (<\/strong>\u8ca8\u865f<\/strong>T0<\/strong>015<\/strong>)<\/strong><\/p>\n

SV40 Immortalized Rat Cardiomyocytes\u7d30\u80de\u682a | \u4e0d\u6747\u65b9\u6cd5: Serial passaging and transduction with recombinant lentivector carrying SV40 Large T antigen | \u4f86\u6e90Heart\/neonatal | \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a><\/span>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIII (\u8ca8\u865fTM003<\/a><\/span>)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a><\/span>)\u6700\u7d42\u6fc3\u5ea6\u70ba15%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C.<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u89e3\u51cd\u7d30\u80de\u682a\u6d41\u7a0b<\/strong><\/p>\n

\"\"<\/p>\n

1. \u7d30\u80de\u89e3\u51cd\u524d\u8655\u7406: 37\u00b0C\u6c34\u6d74\u4e2d\u9810\u71b1\u5b8c\u5168\u57f9\u990a\u57fa\u3002\u6e96\u5099\u597d\u542b\u67095mL\u5b8c\u5168\u57f9\u990a\u57fa\u768415mL\u7121\u83cc\u9310\u5f62\u7ba1<\/p>\n

2.\u5f9e\u6db2\u6c2e\u5132\u7f50\u4e2d\u53d6\u51fa\u51b7\u51cd\u4fdd\u5b58\u7684\u5c0f\u74f6\u3002<\/p>\n

3. \u7d30\u80de\u89e3\u51cd: \u5c07\u5c0f\u74f6\u7684\u4e0b\u534a\u90e8\u5206\u653e\u516537\u00b0C\u6c34\u6d74\u4e2d\u5feb\u901f\u89e3\u51cd\u7d30\u80de\uff0c60\u79d2\u5f8c\u53d6\u51fa\u3002 \u89e3\u51cd\u5f8c\u4ecd\u61c9\u4fdd\u7559\u5c11\u91cf\u51b0\u6676\u3002\u3010\u7531\u65bcDMSO\u7684\u5b58\u5728\u5c0d\u7d30\u80de\u6709\u6bd2\u6027\uff0c\u56e0\u6b64\u91cd\u8981\u7684\u662f\u4e0d\u8981\u904e\u5ea6\u878d\u5316\u51b7\u51cd\u7ba1\u3011\u3002<\/p>\n

4.\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u5728\u5c0f\u74f6\u4e2d\uff0c\u4e26\u5c07\u7d30\u80de\u61f8\u6db2\u8f49\u79fb\u5230\u542b\u67095mL\u5b8c\u5168\u57f9\u990a\u57fa\u768415mL\u7121\u83cc\u9310\u5f62\u7ba1\u4e2d\u3002<\/p>\n

5.\u4ee5200xg\u96e2\u5fc3\u7d30\u80de3\u5206\u9418\u4ee5\u6c89\u6fb1\u3002<\/p>\n

6. \u6e96\u5099\u597dCulture dish<\/p>\n

7. \u5c07\u96e2\u5fc3\u5f8c\u4e4b\u7d30\u80de, \u5438\u6389\u57f9\u990a\u57fa (\u542bDMSO)\uff0c\u4f7f\u7d30\u80de\u6c89\u6fb1\u4e0d\u53d7\u5e72\u64fe\u3002<\/p>\n

8.\u5c07\u65b0\u9bae\u57f9\u990a\u57fa\u4e2d\u7684\u6c89\u6fb1\u7269 (\u7d30\u80de) \u91cd\u65b0\u61f8\u6d6e\u4e26\u5728\u65b0\u57f9\u990a\u5bb9\u5668\u4e2d\u57f9\u990a\u3002<\/p>\n

9.\u572837\u2103\uff0c5\uff05CO2\u4e0b\u57f9\u990a\u57f9\u990a\u7269\u3002<\/p>\n

\u6ce8\u610f\uff1a\u5728\u9069\u7528\u7684\u60c5\u6cc1\u4e0b\uff0c\u6211\u5011\u5efa\u8b70\u7d30\u80de\u5f9e\u89e3\u51cd\u5f8c\u6062\u5fa9\u5f8c\u9078\u64c7\u85e5\u7269\u6dfb\u52a0\u5291\u3002 \u5728\u89e3\u51cd\u6642\u76f4\u63a5\u6dfb\u52a0\u9078\u64c7\u85e5\u7269\u53ef\u80fd\u5c0e\u81f4\u61c9\u6fc0\u7d30\u80de\u548c\u751f\u5b58\u529b\u964d\u4f4e\u3002<\/p>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

Zhang Y, Nuglozeh E, Tour\u00e9 F, Schmidt AM, Vunjak-Novakovic G. (2009). Controllable expansion of primary cardiomyocytes by reversible immortalization. Hum Gene Ther. 2009 Dec;20(12):1687-96.<\/p>\n

Devanathan, S\u00a0et al<\/i>. “Sexual dimorphism in myocardial acylcarnitine and triglyceride metabolism.” Biology of Sex Differences 7: (2016).\u00a0DOI: 10.1186\/s13293-016-0077-7<\/a>. Application: Reactive oxygen species.<\/p>\n

Ponce, D\u00a0et al<\/i>. “Comparative study of the toxic effects of\u00a0Chrysaora quinquecirrha\u00a0(Cnidaria: Scyphozoa) and\u00a0Chironex fleckeri(Cnidaria: Cubozoa) venoms using cell-based assays.” Toxicon 106:57-67 (2015).\u00a0DOI: http:\/\/dx.doi.org\/10.1016\/j.toxicon.2015.09.014<\/a>.\u00a0PubMed: 26385314<\/a>. Application: cytotoxic assay.<\/p>\n

\u7522\u54c1\u8cc7\u6599\u8acb\u6309\u9019<\/span>\u00a0PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a>\u00a0<\/span><\/strong><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

Immortalized Rat Cardiomyocytes \u00a0| Immortalized Rat Cell Lines | Immortalized Heart Cells \u00a0| Immortalized Primary Cells | abm Inc.<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

HOT !! \u4e0d\u6764\u5316\u7cfb\u5217\u5546\u54c1<\/span> |<\/span> Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a>|\u00a0Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus\u75c5\u6bd2RT-QPCR\u5b9a\u91cf(\u5b9a\u91cf)<\/a>\u00a0|\u00a0Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf\u5361)<\/a><\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":8001,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453,455],"tags":[4124],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8000"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8000"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8000\/revisions"}],"predecessor-version":[{"id":50873,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8000\/revisions\/50873"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8001"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8000"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8000"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8000"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}