{"id":7996,"date":"2016-12-06T09:34:19","date_gmt":"2016-12-06T01:34:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7996"},"modified":"2025-08-14T15:51:54","modified_gmt":"2025-08-14T07:51:54","slug":"5mins-stripping-buffer-%e6%8a%97%e9%ab%94%e6%8a%97%e5%8e%9f%e5%8e%bb%e9%99%a4%e6%b6%b2-%e8%b2%a8%e8%99%9f-jb11-k002","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7996","title":{"rendered":"5mins Stripping buffer | \u6297\u9ad4\u6297\u539f\u53bb\u9664\u6db2 \u8ca8\u865f JB11-K002"},"content":{"rendered":"

\u3010Membrane stripping for western blot\u3011<\/span><\/strong>\u897f\u65b9\u58a8\u9ede\u6cd5\u4e4b\u76f8\u95dc\u7de9\u885d\u6db2 -Stripping buffer<\/span><\/p>\n

Western Blot Stripping Buffer | 500ml T-PRO \u8ca8\u865fJB11-K002<\/span><\/a><\/span><\/p>\n

5 mins \u5feb\u901f\u6297\u9ad4\u6297\u539f\u53bb\u9664\u6db2 Western Blot Stripping Buffer (JB11-K002) Stripping buffer\u76ee\u7684\u7528\u65bcWestern Blot\u4e2d\uff0c\u53bb\u9664\u5df2\u7d93\u7528\u5316\u5b78\u767c\u5149\u7684\u6297\u9ad4\uff1b\u518d\u91cd\u65b0\u63a2\u6e2c\u3002\u53bb\u9664\u819c\u53ef\u4ee5\u662f\u785d\u5316\u7e96\u7dad\u7d20\u6216PVDF \/\u5c3c\u9f8d\u3002\u7522\u54c1\u7279\u9ede: 5~15\u5206\u9418\u5373\u53ef\u5b8c\u6210\u5370\u8de1\u525d\u96e2\u3002\u7121\u9808\u52a0\u71b1, \u53ea\u9700\u5ba4\u6eab\u5b75\u80b2\u3002\u7121\u60e1\u81ed\u6c23\u5473\uff0c\u4e14\u4e0d\u6dfb\u52a02-mercaptoethanol\u6216\u5176\u985e\u4f3c\u7269\u3002\u63a1\u7528\u74b0\u4fdd\u7121\u5bb3\u7684\u6709\u6a5f\u6eb6\u5291\uff08\u6c23\u5473\u8f15\u5fae\uff09\u7684\u5c08\u6709\u914d\u65b9\uff0c\u53ef\u5b8c\u5168\u5206\u96e2\u7d50\u5408\u7684\u6297\u9ad4\u3002\u5f48\u6027\u61c9\u7528\u5ee3- \u00a0NC\u6216PVDF\u8f49\u6f2c\u819c\u7686\u9069\u7528\u3002Stripping \u4e26\u91cd\u65b0\u6aa2\u6e2c\u86cb\u767d\u8cea\u5370\u8de1 ( western blot) \u662f\u4e00\u7a2e\u5c07\u4e00\u6297\u548c\u4e8c\u6297\u5f9e\u86cb\u767d\u8cea\u5370\u8de1\u4e2d\u53bb\u9664\uff0c\u4ee5\u4fbf\u91cd\u65b0\u6aa2\u6e2c\u5370\u8de1\u7684\u65b9\u6cd5\u3002blot\u53ef\u4ee5\u525d\u96e2\u4e26\u91cd\u65b0\u6aa2\u6e2c\u591a\u6b21\uff0c\u4ee5\u986f\u793a\u5176\u4ed6\u86cb\u767d\u8cea\u6216\u512a\u5316\u5c0d\u67d0\u7a2e\u86cb\u767d\u8cea\u7684\u6aa2\u6e2c\uff08\u4f8b\u5982\uff0c\u6297\u9ad4\u6fc3\u5ea6\uff09\uff0c\u800c\u7121\u9700\u591a\u6b21\u51dd\u81a0\u96fb\u6cf3\u548c\u8f49\u5370\u3002<\/p>\n

T-Pro Western Blot \u525d\u96e2\u8a66\u5291\u4f7f\u7528\u65b9\u6cd5<\/span><\/p>\n

\u2022 \u5c0710 ml\u525d\u96e2\u8a66\u5291\u5012\u5165\u4e7e\u6de8\u7684\u5bb9\u5668\u4e2d\uff0c\u4e26\u5c07\u819c\u653e\u5165\u5176\u4e2d\u3002\u78ba\u4fdd\u819c\u5b8c\u5168\u6d78\u6c92\u5728\u525d\u96e2\u7de9\u885d\u6db2\u4e2d\u3002<\/p>\n

\u2022 \u5c07\u819c\u5728\u5ba4\u6eab\u4e0b\u8207\u525d\u96e2\u8a66\u5291\u4e00\u8d77\u5b75\u80b23\u5206\u9418\uff0c\u4e26\u5287\u70c8\u652a\u62cc\u5169\u6b21\u3002\u5c0d\u65bc\u9ad8\u89aa\u548c\u529b\u6297\u9ad4\uff0c\u5b75\u80b2\u6642\u9593\u9700\u8981\u6700\u4f73\u5316\uff0c\u901a\u5e38\u5c0d\u65bc\u5927\u591a\u6578\u6297\u9ad4\uff0c37\u2103\u4e0b\u525d\u96e25~10\u5206\u9418\u5373\u53ef\u3002<\/p>\n

\u2022 \u5728\u5ba4\u6eab\u4e0b\u4f7f\u7528\u5927\u91cf\uff08\u4f8b\u5982 100 \u6beb\u5347\uff09\u7684 TBS-T \u6216 PBS-T \u6e05\u6d17\u819c\u5169\u6b21<\/p>\n

\u4ec0\u9ebc\u662f\u819c\u525d\u96e2 (membrane stripping)\uff1f<\/span><\/p>\n

\u819c\u525d\u96e2 (Membrane stripping) \u662f\u5f9e\u86cb\u767d\u8cea\u5370\u8de1\u819c\u4e0a\u53bb\u9664\u4e00\u6297\u548c\u4e8c\u6297\u7684\u904e\u7a0b\u3002\u5176\u76ee\u7684\u662f\u5728\u4e0d\u5207\u958b\u819c\u7684\u60c5\u6cc1\u4e0b\u5728\u540c\u4e00\u5370\u8de1\u4e0a\u7814\u7a76\u591a\u7a2e\u86cb\u767d\u8cea\u3002\u819c\u525d\u96e2 (Membrane stripping) \u5728\u6aa2\u6e2c\u76ee\u6a19\u86cb\u767d\u8cea\u7684\u540c\u6642\u4f7f\u7528\u5408\u9069\u7684\u4e0a\u6a23\u5c0d\u7167\uff0c\u6216\u7814\u7a76\u5169\u7a2e\u5206\u5b50\u91cf\u76f8\u8fd1\u7684\u86cb\u767d\u8cea\u6642\u5c24\u5176\u6709\u7528\u3002\u819c\u525d\u96e2 (Membrane stripping) \u4e5f\u5728\u5176\u4ed6\u60c5\u6cc1\u4e0b\u975e\u5e38\u6709\u7528\u3002\u4f8b\u5982\uff0c\u7576\u4f7f\u7528\u4e86\u932f\u8aa4\u7684\u6297\u9ad4\uff0c\u6216\u7531\u65bc\u80cc\u666f\u8a0a\u865f\u904e\u9ad8\u800c\u5fc5\u9808\u91cd\u65b0\u9032\u884c\u819c\u5c01\u9589\u6642\uff0c\u819c\u525d\u96e2 (Membrane stripping) \u975e\u5e38\u6709\u7528\u3002\u900f\u904estripping\u4e26\u91cd\u65b0\u6aa2\u6e2c\u55ae\u500b\u8584\u819c\uff0c\u60a8\u53ef\u4ee5\u7bc0\u7701\u6a23\u54c1\u548c\u6642\u9593\uff0c\u907f\u514d\u904b\u884c\u548c\u5370\u8de1\u591a\u500b\u51dd\u81a0\u3002\u907f\u514d\u5c0d\u525d\u96e2\u524d\u5f8c\u6aa2\u6e2c\u7684\u6a19\u9776\u9032\u884c\u5b9a\u91cf\u6bd4\u8f03\uff0c\u56e0\u70ba\u8a72\u64cd\u4f5c\u6703\u5f9e\u819c\u4e0a\u53bb\u9664\u4e00\u4e9b\u6a23\u54c1\u86cb\u767d\u8cea\u3002\u57fa\u65bc\u540c\u6a23\u7684\u539f\u56e0\uff0c\u4e0d\u61c9\u6aa2\u6e2c\u525d\u96e2\u5f8c\u7684\u819c\u4f86\u8b49\u660e\u86cb\u767d\u8cea\u7684\u7f3a\u5931\uff0c\u4f8b\u5982\u7528\u65bc\u6572\u9664\u6e2c\u8a66\u6216RNAi\u5be6\u9a57\u3002\u6211\u5011\u5efa\u8b70\u4f7f\u7528 PVDF \u819c\u4f86\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u6a23\u54c1\u86cb\u767d\u8cea\u7684\u640d\u5931\uff0c\u56e0\u70ba\u785d\u9178\u7e96\u7dad\u7d20\u819c\u5f80\u5f80\u6703\u6709\u66f4\u56b4\u91cd\u7684\u86cb\u767d\u8cea\u640d\u5931\u3002<\/p>\n

\u897f\u65b9\u58a8\u9ede\u6cd5\u4e4b\u76f8\u95dc<\/strong><\/span>\u5546\u54c1<\/span>\u00a0<\/strong>Isotype control <\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong><\/a> | <\/strong>\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/strong><\/a> | <\/strong>\u6297\u9ad4\u5206\u578b<\/strong>ELISA <\/strong>\u5957\u7d44<\/strong><\/a> | <\/strong>anti-rabbit <\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong>anti-mouse<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> |<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/tr>\n
JB02-B006S<\/td>\nT-Pro EZ Gel Solution 6%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B006M<\/td>\nT-Pro EZ Gel Solution 6%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B008S<\/td>\nT-Pro EZ Gel Solution 8%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B008M<\/td>\nT-Pro EZ Gel Solution 8%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B010S<\/td>\nT-Pro EZ Gel Solution 10%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B010M<\/td>\nT-Pro EZ Gel Solution 10%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B012S<\/td>\nT-Pro EZ Gel Solution 12%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B012M<\/td>\nT-Pro EZ Gel Solution 12%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB02-B015S<\/td>\nT-Pro EZ Gel Solution 15%<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JB02-B015M<\/td>\nT-Pro EZ Gel Solution 15%<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB03-C001<\/td>\nT-Pro Separating or Resolving Buffer<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB03-C002<\/td>\nT-Pro Stacking Buffer<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB04-D001<\/td>\nT-Pro BCA Protein Assay kit<\/td>\n500assay\/KIT<\/td>\n<\/tr>\n
JB04-D001A<\/td>\nT-Pro BCA Protein Assay Reagent A<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB04-D001B<\/td>\nT-Pro BCA Protein Assay Reagent B<\/td>\n12ml\/BT<\/td>\n<\/tr>\n
JB04-D001C<\/td>\nAlbumin Standard (2.0 mg\/ml)<\/td>\n5*1ml\/kit<\/td>\n<\/tr>\n
JB04-D002<\/td>\nT-Pro Bradford\u00a0 Protein Assay kit (1X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB06-F002<\/td>\nT-Pro Laemmli (SDS sample) Reagent (reducing 4X)<\/td>\n10ml\/BT<\/td>\n<\/tr>\n
JB06-F003<\/td>\nT-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)<\/td>\n10ml\/BT<\/td>\n<\/tr>\n
JB07-G001<\/td>\nT-Pro Tris-Glycine-SDS running buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB07-G002<\/td>\nT-Pro Tris-Glycine-Native running buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB07-G003<\/td>\nT-Pro MOPS\/SDS running buffer (20X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB08-H001<\/td>\nT-Pro Transfer Blotting buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB08-H002<\/td>\nT-Pro Semi Dry Transfer Blotting buffer (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I001<\/td>\nT-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I002<\/td>\nT-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I003<\/td>\nT-Pro Washing buffer in PBS and Tween-20 (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB09-I004<\/td>\nT-Pro Washing buffer in TBS and Tween-20 (10X)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JB11-K002<\/td>\nT-Pro Western Blot Stripping Reagent<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT90-R001S<\/td>\nT-Pro Aqua EZ Clean (S)<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JT90-R001M<\/td>\nT-Pro Aqua EZ Clean (M)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT90-R002<\/td>\nT-Pro MycoClean spray<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT90-R005S<\/td>\nT-Pro EZ stain Gel solution<\/td>\n100ml\/BT<\/td>\n<\/tr>\n
JT90-R005M<\/td>\nT-Pro EZ stain Gel solution<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W001<\/td>\nT-Pro Fast Blocking Buffer (in PBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W002<\/td>\nT-Pro Fast Blocking Buffer (in TBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W003<\/td>\nT-Pro Protein Free Blocking Buffer (in PBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JK92-W004<\/td>\nT-Pro Protein Free Blocking Buffer (in TBS)<\/td>\n500ml\/BT<\/td>\n<\/tr>\n
JT96-K002S<\/td>\nT-Pro LumiFast Plus ECL Kit<\/td>\n100ml*2\/Kit<\/td>\n<\/tr>\n
JT96-K002M<\/td>\nT-Pro LumiFast Plus ECL Kit<\/td>\n250ml*2\/Kit<\/td>\n<\/tr>\n
JT96-K004S<\/td>\nT-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)<\/td>\n100ml*2\/Kit<\/td>\n<\/tr>\n
JT96-K004M<\/td>\nT-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)<\/td>\n250ml*2\/Kit<\/td>\n<\/tr>\n
JT97-N001M<\/td>\nT-Pro Nonliposomal Transfection Reagent I (NTR I)<\/td>\n1.0ml\/vial<\/td>\n<\/tr>\n
JT97-N002M<\/td>\nT-Pro Nonliposomal Transfection Reagent II (NTR II)<\/td>\n1.0ml\/vial<\/td>\n<\/tr>\n
JT97-N006M<\/td>\nT-Pro Nonliposomal Transfection Reagent III (NTR III)<\/td>\n1.0ml\/vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010Membrane stripping for western blot\u3011\u897f\u65b9\u58a8\u9ede\u6cd5\u4e4b\u76f8\u95dc\u7de9\u885d\u6db2 -Strip […]<\/p>\n","protected":false},"author":3,"featured_media":6997,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89,135],"tags":[4125],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7996"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7996"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7996\/revisions"}],"predecessor-version":[{"id":7998,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7996\/revisions\/7998"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6997"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7996"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7996"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7996"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}