{"id":7943,"date":"2016-12-02T16:21:02","date_gmt":"2016-12-02T08:21:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7943"},"modified":"2024-04-26T11:47:54","modified_gmt":"2024-04-26T03:47:54","slug":"oligonucleotides-%e5%af%a1%e6%a0%b8%e9%85%b8%e6%8a%97%e9%ab%94%e6%a8%99%e8%aa%8c%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9f425-0000","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7943","title":{"rendered":"\u5be1\u6838\u9178\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 | Oligonucleotides labeling kit\u8ca8\u865f425-0000 (abcam ab218260)"},"content":{"rendered":"

Thunder-Link\u00ae\u00a0Oligonucleotides <\/span><\/strong>\u5be1\u6838\u9178\u6a19\u8a8c\u5957\u7d44 \u8ca8\u865f425-0000 (abcam ab218260)<\/strong><\/span><\/a><\/span><\/p>\n

\u5feb\u901f\u6297\u9ad4\u8207\u5be1\u6838\u82f7\u9178\u7d50\u5408<\/p>\n

\u6297\u9ad4(antibodies) -\u5be1\u6838\u82f7\u9178 (oligonucleotides)\u6a19\u8a8c\uff0c\u662f\u65b0\u4e00\u4ee3\u751f\u7269\u6a19\u8a8c\u7269\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u53ef\u514b\u670d\u9748\u654f\u5ea6\u548c\u7dda\u6027\u7bc4\u570d\u554f\u984c\uff0c\u53ef\u80fd\u6210\u70ba\u591a\u91cd\u86cb\u767d\u8cea\u8a3a\u65b7\u5206\u6790\u4e2d\u4e4b\u5e73\u53f0\u3002Thunder-Link \u00ae PLUS \u6297\u9ad4\u6a19\u8a8c\u5957\u7d44\uff0c\u5feb\u901f\u5c07\u6297\u9ad4(antibodies)\u8207\u5be1\u6838\u82f7\u9178(oligonucleotides)\u4ee5\u5171\u50f9(Covalent bond)\u7a69\u5b9a\u6027\u7d50\u5408\uff0c\u00a095-100%\u56de\u6536\u7387\u9ad8\uff0c\u53ef\u61c9\u7528\u65bc\u514d\u75abPCR\uff08Immuno-PCR\uff09\u3001\u63a5\u8fd1\u9023\u63a5\u6e2c\u5b9a(Proximity Ligation Assay)\u3001\u96fb\u5316\u5b78\u9130\u8fd1\u6e2c\u5b9a (Electrochemical Proximity Assay) CITEseq \u53ca REAPseq\u3002\u8207\u50b3\u7d71ELISA\u65b9\u6cd5\u76f8\u6bd4\uff0c\u514d\u75abPCR\uff08Immuno-PCR\uff09\u5927\u5e45\u63d0\u9ad8\u5075\u6e2c\u6975\u9650\u3002<\/p>\n

\"\"<\/p>\n

Thunder-Link \u00ae PLUS \u64cd\u4f5c\u6d41\u7a0b:<\/p>\n

\u6b64\u7522\u54c1\u53ef\u5c07oligo \u6a19\u8a8c\u65bc\u6297\u9ad4\/Protein\/peptide\u5206\u5b50\u3002\u9996\u5148\u5c07\u6297\u9ad4\u53ca\u80fa\u57fa\u5316\u4e4boligo\uff0c\u5404\u5225\u52a0\u5165activation buffer \u5f8c\uff0c30\u5206\u5f8c\u5404\u5225\u5f62\u6210Activation\u578b\u5f0f\uff0c\u9032\u884c1\u5c0f\u6642\u53cd\u61c9\u3002\u7d93\u7531desalt\u591a\u9918\u96e2\u5b50\u5f8c\uff0c\u5c07\u6297\u9ad4\u8207oligo\u6df7\u5408\u5728\u4e00\u8d77\u9032\u884c\u7d50\u5408\uff0c\u6700\u5f8c\u7d93\u7531\u4f4e\u901f\u96e2\u5fc3\u53ca\u6c89\u6fb1\u6b65\u9a5f\uff0c\u7d14\u5316\u6297\u9ad4-oligo\u8907\u5408\u7269\u3002\u5be1\u6838\u82f7\u9178(oligonucleotides)<\/strong>\u76843’\u62165’\u7aef\u9032\u884c-NH2\u4fee\u98fe\u8a2d\u8a08\uff0c\u9577\u5ea6\u70ba10-120\u500b\u9e7c\u57fa\u7684\u55ae\u93c8\u5be1\u6838\u82f7\u9178\/80\u500b\u9e7c\u57fa\u5c0d (bases) \u7684\u96d9\u93c8\u5be1\u6838\u82f7\u9178(double stranded oligos )\uff0cpH6\u548cpH8\uff0cHPLC\u7d14\u5316\uff0c60-100\u00b5M (high)\u6fc3\u5ea6\u3002\u7de9\u885d\u6db2\u4e0d\u5f97\u542b\u6709\u80fa\u57fa\u5718 (primary amine groups)\uff0c \u61c9\u907f\u514d\u6dfb\u52a0BSA\u548c\u758a\u6c2e\u5316\u7269(azide) \u7b49\u6dfb\u52a0\u5291 (\u53ef\u5c07\u5be1\u805a\u7269\u6eb6\u89e3\u5728pH7\u7684\u78f7\u9178\u9e7d\u7de9\u885d\u6db2(\u00a0phosphate buffer )\u4e2d\u3002\u00a0\u6297\u9ad4\u6bcf\u6b21\u53cd\u61c9\u70ba100 ul (1mg\/ml) \u300230\u5206\u6d3b\u5316\u6297\u9ad4(antibodies)\u8207\u5be1\u6838\u82f7\u9178(oligonucleotides)\uff0c\u9032\u884c1\u5c0f\u6642\u53cd\u61c9\uff0c\u4f7f\u7528\u5167\u9644Separation column\u5feb\u901f\u79fb\u53bb\u672a\u7d50\u5408\u7684\u5be1\u6838\u82f7\u9178\u7684\u5e72\u64fe\u3002\u5957\u7d44\u5167\u9644\u6709\u967d\u6027\u5c0d\u7167\u6297\u9ad4(control antibody)\u548c\u5be1\u6838\u82f7\u9178(control oligo)\u3001\u00a0Oligo Activation reagent\u3001\u00a0Antibody Activation Reagent\u3001\u00a0Oligo Activation reagent\u3001separation column\/Wash buffer\/\u00a0Conjugate Clean Up Reagent\/Antibody Suspension Buffer\u3002(\u8acb\u53c3\u8003\u64cd\u4f5c\u624b\u518a)<\/p>\n

\"\"<\/p>\n

Immuno-PCR\u61c9\u7528<\/strong><\/p>\n

\u514d\u75abPCR\u7d50\u5408oligonucleotide\u65bcImmunoassay\u4e2d\uff0c\u5927\u5e45\u63d0\u9ad8\u5075\u6e2c\u6975\u9650\u3002\u5728\u6e2c\u8a66\u6a23\u54c1\u4f8b\u5982\u5168\u8840\u548c\u8840\u6e05\u4e2d\uff0c\u53ef\u7cbe\u78ba\u548c\u9748\u654f\u5730\u6aa2\u6e2c\u7a00\u6709\u7d30\u80de\u6216\u4f4e\u6fc3\u5ea6\u7684\u86cb\u767d\u8cea\u3002\u514d\u75abPCR\u64f4\u589e\uff0c\u5e38\u898f\u6aa2\u6e2c\u55ae\u500b\u6838\u9178\u5206\u5b50\u3002\u8207\u5176\u6a21\u677f\u96dc\u4ea4\u7684DNA\u63a2\u91dd\u8a2d\u8a08\uff0c\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u548c\u9ad8\u7279\u7570\u6027\u4e4b\u65b9\u6cd5\u3002<\/p>\n

 <\/p>\n

INNOVA\u63d0\u4f9b\u5169\u7a2e\u5546\u54c1\uff0c\u53ef\u5c07\u5be1\u6838\u82f7\u9178\u9023\u63a5\u5230\u4e0d\u540c\u7d44\u7684\u5206\u5b50\uff08\u4f8b\u5982\u6297\u9ad4\u6216\u91d1\u7d0d\u7c73\u9846\u7c92\uff09\uff0c\u8b93\u514d\u75ab\u6e2c\u5b9a\u66f4\u591a\u5143\u5316\u3002Immuno-PCR\u9748\u654f\u5ea6\u589e\u52a010\u81f31000\u500d\u3002\u4ea6\u53ef\u505a\u70ba\u591a\u91cd\u86cb\u767d\u8cea\u8a3a\u65b7\u6e2c\u5b9a\u7684\u5e73\u53f0\u3002\u4e0b\u5716\u986f\u793a\u4e86\u5728\u7279\u5b9a\u6297\u539f\u6fc3\u5ea6\u4e0b\u7531SYBR Green\u7522\u751f\u7684PCR\u63a2\u91dd\u7522\u751f\u7684PCR\u5faa\u74b0\u6b21\u6578\u8207\u87a2\u5149\u5f37\u5ea6\u7684\u95dc\u4fc2\u5716\u3002 \u4e0b\u5716\u986f\u793a\u4e86\u8f49\u63db\u70ba\u6297\u539f\u91cf\u8207\u5faa\u74b0\u6b21\u6578\u5f8c\u7684\u6578\u64da\uff0c\u4ee5\u4fbf\u80fd\u5920\u8a08\u7b97\u6a19\u6e96\u66f2\u7dda\u3002 \u7d50\u679c\u986f\u793a\u8a72\u6e2c\u5b9a\u4f7f\u7528\u7684\u6355\u7372\u6297\u9ad4\u5c111000\u500d\uff0c\u6aa2\u6e2c\u6297\u9ad4\u5c11100\u500d\uff0c\u9748\u654f\u5ea6\u6bd4\u540c\u7b49ELISA\u9ad81000\u500d\u3002<\/p>\n

Proximity ligation assay-Used to identify proteins co-located within cells.<\/strong><\/p>\n

 <\/p>\n

Proximity extension assay-Variation on ImmunoPCR using two antibodies specific for different epitopes.<\/strong><\/p>\n

 <\/p>\n

ImmunoPCR Data<\/h3>\n

ImmunoPCR data generated using Thunder-Link\u00ae<\/sup>. A mouse monoclonal antibody specific for human CRP (clone C7) was purchased in unconjugated format from HyTest. The antibody was conjugated to an oligonucleotide using a Thunder-Link\u00ae<\/sup>\u00a0kit, and was used as a detection antibody in a sandwich immunoPCR assay with a polyclonal antiCRP antibody as the capture reagent.<\/p>\n

The top graph shows a plot of the number of PCR cycles undertaken vs. fluorescence intensity generated by SYBR Green containing PCR probes at particular antigen concentrations. The bottom graph shows this data following conversion to antigen amount vs. cycle number to enable the calculation of a standard curve. The results show that the assay utilizes 1000-fold less capture antibody and 100-fold less detection antibody, and provides 1000-fold greater sensitivity than the equivalent ELISA<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Features<\/td>\nBenefits<\/td>\n<\/tr>\n
Quick and easy to use<\/td>\nSave time, no specialist knowledge required<\/td>\n<\/tr>\n
High levels of antibody and oligo recovery<\/td>\nSave precious reagents<\/td>\n<\/tr>\n
Use your own oligo and antibody, at your desired ratio<\/td>\nFlexible<\/td>\n<\/tr>\n
Freeze dried<\/td>\nShips at ambient temperature, long shelf-life<\/td>\n<\/tr>\n
Stringently QC tested<\/td>\nConsistent high quality, excellent batch-to-batch reproducibility<\/td>\n<\/tr>\n
Unidirectional chemistry<\/td>\nNo risk of cross-linking<\/td>\n<\/tr>\n
Covalent bond<\/td>\nHighly stable conjugates<\/td>\n<\/tr>\n
Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairs<\/td>\nCovers the majority of sequences<\/td>\n<\/tr>\n
Linking chemistry works at both 5\u2019 and 3\u2019 end<\/td>\nProvides ability to combine with other modifications<\/td>\n<\/tr>\n
Post-conjugation clean-up step<\/td>\nNo interference from unbound oligo<\/td>\n<\/tr>\n
Positive control antibody and oligo included<\/td>\nEnables confirmation of protocol success<\/td>\n<\/tr>\n
Wide range of target proteins<\/td>\nAlso applicable to antibody fragments and small proteins<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
425-0000
\n425-0300<\/td>\n		Thunder-Link\u00ae oligo conjugation system<\/td>\n1 reaction
\n3 reaction<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

\n

Multiplexed quantification of proteins and transcripts in single cells – Nature Biotechnology, 2018<\/h4>\n
\n

Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70 – Epigenetics Chromatin. (2017)<\/h4>\n
\n

Label-free and high-throughput detection of biomolecular interactions using a flatbed scanner biosensor – ACS sens. (2017)<\/h4>\n
\n

Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection – PNAS (2017)<\/h4>\n
<\/div>\n<\/div>\n<\/div>\n

 <\/p>\n<\/div>\n

 <\/p>\n<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Thunder-Link\u00ae\u00a0Oligonucleotides \u5be1\u6838\u9178\u6a19\u8a8c\u5957\u7d44 \u8ca8\u865f425-0000 (abca […]<\/p>\n","protected":false},"author":3,"featured_media":7944,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[197],"tags":[4147],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7943"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7943"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7943\/revisions"}],"predecessor-version":[{"id":49308,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7943\/revisions\/49308"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7944"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7943"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7943"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}