{"id":7921,"date":"2016-12-01T17:58:42","date_gmt":"2016-12-01T09:58:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7921"},"modified":"2023-07-06T16:29:54","modified_gmt":"2023-07-06T08:29:54","slug":"wst-1%e7%b4%b0%e8%83%9e%e5%ad%98%e6%b4%bb%e5%88%86%e6%9e%90%e5%91%88%e8%89%b2%e5%a5%97%e7%b5%84-wst-1-cell-proliferation-colorimetric-assay-kit-k301-500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7921","title":{"rendered":"WST-1\u7d30\u80de\u5b58\u6d3b\u5206\u6790(\u5448\u8272)\u5957\u7d44| WST-1 Cell Proliferation Colorimetric Assay Kit K301-500\uff08abcam ab65473\uff09"},"content":{"rendered":"

Cell Proliferation Assay Kits | \u8ca8\u865fK301-500 \uff08abcam ab65473\uff09<\/a><\/strong><\/span><\/p>\n

Biovision\u00a0<\/strong>\u7cfb\u5217\u7d30\u80de\u5b58\u6d3b | <\/strong>\u7d30\u80de\u6bd2\u6027<\/strong>\u5206\u6790\u5957\u7d44 (\u5448\u8272\/\u87a2\u5149\/\u51b7\u5149)<\/strong><\/span><\/p>\n

WST-1 Cell Proliferation Colorimetric Assay Kit\u00a0WST1\u7d30\u80de\u6d3b\u6027\u5206\u6790\u5448\u8272\u5957\u7d44 (\u8ca8\u865fK301-500)\uff1b\u5feb\u901f\u7d30\u80de\u589e\u6b96\u5206\u6790\uff0c\u4f7f\u7528\u6c34\u6eb6\u6027tetrazolium\u9e7d\u985e\u7684\u7684WST-1\u4f5c\u70ba\u67d3\u5291\uff0c\u5229\u7528\u7c92\u817a\u9ad4\u4e2d\u7684\u812b\u6c2b\u9176\u9084\u539f\u6210\u6a59\u8272\u7522\u7269\u3002WST-1\u6bd4 \u8d77\u50b3\u7d71\u7684MTT\u3001XTT\u6216\u662fMTS-base\u5206\u6790\u8981\u4f86\u7684\u66f4\u5feb\u901f\u4e14\u9748\u654f\u5ea6\u66f4\u9ad8\u3002|\u00a0\u7d30\u80de<\/strong>\u8001\u5316<\/strong>\u5206\u6790<\/strong>\u5957\u7d44<\/strong><\/span><\/a>|Senescence Detection Kit\uff1a \u7d30\u80de\u8001\u5316\u88ab\u8a8d\u70ba\u662f\u6291\u5236\u764c\u7d30\u80de\u7684\u6a5f\u5236\uff0c\u56e0\u6b64\u7576\u7d30\u80de\u8001\u5316\u6642\u96d6\u7136\u7d30\u80de\u6703\u6301\u7e8c\u5b58\u6d3b\uff0c\u4f46\u662f\u5728\u9019\u500b\u968e\u6bb5\u7684\u7d30\u80de\u662f\u4e0d\u6703\u5206\u88c2\uff0c\u4e5f\u7121\u6cd5\u4ee5\u8840\u6e05\u53ca\u57f9\u990a\u57fa\u9032\u884c\u7d30\u80de\u7e6b\u4ee3\u57f9\u990a\u3002Senescence detection kit\u662f\u5075\u6e2c\u5207\u7247\u4e2d\u7d44\u7e54\u6216\u7d30\u80de\u4e0a \u7279\u5b9a\u7684senescence marker\uff0c\u800c\u9019\u4e9bmarker\u662f\u4e0d\u6703\u51fa\u73fe\u5728\u5176\u4ed6\u7684\u7d30\u80de\u4e2d\u3002|\u751f\u7269\u51b7\u5149\u7d30\u80de\u6bd2\u6027\u5206\u6790\u5957\u7d44<\/strong><\/a><\/span> | Bioluminescence Cytotoxicity Assay Kit\uff1a \u9019\u5957\u7d30\u80de\u6bd2\u6bba\u8a66\u5291\u7d44\u662f\u900f\u904e\u53d7\u640d\u50b7\u7684\u7d30\u80de\u91cb\u653eAdenylate Kinase (AK)\uff0c\u5229\u7528AK\u5c07phosphorylates ADP \u8f49\u5316\u70ba ATP\uff0c\u7522\u751f\u7684 ATP \u80fd\u5920\u53c3\u8207 Luciferase \u51b7\u5149\u53cd\u61c9\uff0c\u518d\u900f\u904e\u51b7\u5149\u5075\u6e2c\u5100\u5075\u6e2c\u7d50\u679c\uff0c\u6bcf\u6b21\u5206\u6790\u53ea\u8981 10 \u500b\u4ee5\u4e0a\u7d30\u80de\u5c31\u80fd\u5206\u6790\uff0c\u800c\u4e14\u53ea\u898110\u5206\u5c31\u80fd\u5f97\u5230\u7d50\u679c\u3002|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u5206\u6790\u5957\u7d44<\/a><\/strong>\u00a0<\/span>|LDH-Cytotoxicity Assay Kit\uff1a \u9019\u500b\u8a66\u5291\u7d44\u662f\u900f\u904e\u5075\u6e2c\u4e73\u9178\u812b\u6c2b\u9176(lactate dehydrogenase, LDH)\u6d3b\u6027\u5224\u65b7\u7d30\u80de\u6bd2\u6bba\u73fe\u8c61\u3002LDH\u5b58\u5728\u65bc\u6240 \u6709\u7d30\u80de\u4e2d\uff0c\u7576\u7d30\u80de\u7834\u88c2\u6642\u624d\u6703\u91cb\u653e\u81f3\u7d30\u80de\u5916\u3002\u4f7f\u7528LDH-cytotoxicity assay kit\u53ea\u9700\u8981\u5206\u5149\u5149\u5ea6\u8a08500nm \u6ce2\u9577\u7684\u5340\u6bb5\uff0c\u907f\u95a7\u653e\u5c04\u7dda\u5206\u6790\u65b9\u6cd5\uff0c\u53ef\u7528\u65bc\u8cbc\u9644\u578b\u6216\u61f8\u6d6e\u578b\u7d30\u80de\u57f9\u990a\u6db2\u3002\u64cd\u4f5c\u6642\u9593\u5927\u7d0430-60\u5206\u9418\u3002|\u6b7b\u6d3b\u7d30\u80de\u67d3\u8272<\/a><\/span> |\u00a0<\/strong>Live-Dead Cell Staining Kit\uff1a Live-Dead Cell Staining Kit\u662fready-to-use\u7684\u8a66\u5291\uff0c\u88e1\u9762\u542b2\u7a2e\u4e0d\u540c\u6ce2\u9577\u7684\u67d3\u5291\uff0c\u5305\u62ec\u67d3\u6d3b\u7d30\u80de\u7684Live -dye (\u7da0\u87a2\u5149\uff0cEx\/Em = 490\/515 nm)\u4ee5\u53ca\u67d3\u6b7b\u7d30\u80de\u7684propidium iodide (PI\uff0c\u7d05\u87a2\u5149\uff0cEx\/Em = 488\/615 nm)\uff0c\u7d30\u80de\u7d93\u904e\u67d3\u8272\u5f8c\uff0c\u80fd\u5920\u76f4\u63a5\u5728\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u7d30\u80de\u5b58\u6d3b\u7684\u72c0\u6cc1\u3002<\/div>\n
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\u7522\u54c1\u4ecb\u7d39:<\/strong><\/div>\n
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\u5feb\u901f\u7d30\u80de\u589e\u6b96\u5206\u6790\u8a66\u5291\u76d2 (The Quick Cell Proliferation Assay Kit , K301) \u7528\u65bc\u5feb\u901f\uff0c\u9748\u654f\u5730\u5b9a\u91cf\u7d30\u80de\u589e\u6b96 (\u00a0cell proliferation)<\/span> \u548c\u6d3b\u529b (viability)<\/span>\u00a0\u3002\u539f\u7406\u70ba\u5229\u7528\u7d30\u80de\u7c92\u817a\u9ad4\u812b\u6c2b\u9176 (\u00a0mitochondrial dehydrogenases) \u5c07\u56db\u5511\u9e7dWST-1 (\u00a0tetrazolium salt WST-1 ) \u88c2\u89e3\u6210\u00a0formazan\u3002\u6d3b\u7d30\u80de\u6578\u91cf\u589e\u52a0\u6703\u5c0e\u81f4\u7dda\u7c92\u9ad4\u812b\u6c2b\u9176 (mitochondrial dehydrogenases) \u6d3b\u6027\u7684\u589e\u52a0\uff0c\u800c\u5f62\u6210\u7684formazan dye\u7684\u91cf\u589e\u52a0\u3002\u6d3b\u7d30\u80de\u7522\u751f\u7684 formazan dye\u53ef\u4ee5\u901a\u904e\u591a\u5b54\u5206\u5149\u5149\u5ea6\u8a08\uff08\u5fae\u91cfReader\uff09\u901a\u904e\u6e2c\u91cf\u67d3\u6599\u6eb6\u6db2\u5728440nm\u8655\u7684\u5438\u5149\u5ea6\u4f86\u5b9a\u91cf\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u61c9\u7528\u65bc\u6e2c\u91cf\u751f\u9577\u56e0\u5b50 (growth factors) \u65bc\u7d30\u80de\u589e\u6b96\u4e4b\u53cd\u61c9\uff0c\u7d30\u80de\u56e0\u5b50(cytokines) \uff0c\u4fc3\u5206\u88c2\u539f(mitogens) \u548c\u71df\u990a\u7d20 (nutrients) \u7b49\u7684\u7d30\u80de\u589e\u6b96\u00a0(\u00a0cell proliferation)\u00a0\u3002\u5b83\u9084\u53ef\u7528\u65bc\u5206\u6790\u7d30\u80de\u6bd2\u6027\u5316\u5408\u7269\uff0c\u5982\u6297\u764c\u85e5\u7269\u548c\u8a31\u591a\u5176\u4ed6\u6bd2\u6027\u5291\u548c\u85e5\u7269\u5316\u5408\u7269\u3002\u65b0\u65b9\u6cd5\u975e\u5e38\u7c21\u55ae\uff0c\u7121\u9700\u6d17\u6ecc\uff0c\u7121\u9700\u6536\u7a6b\uff0c\u4e5f\u7121\u9700\u6eb6\u89e3\u6b65\u9a5f\uff0c\u4e26\u4e14\u6bd4MTT\uff0cXTT\u6216\u57fa\u65bcMTS\u7684\u5206\u6790\u66f4\u5feb\uff0c\u66f4\u9748\u654f\u3002\u6574\u500b\u6e2c\u5b9a\u53ef\u4ee5\u5728\u5fae\u91cf\u6ef4\u5b9a\u677f\u4e2d\u9032\u884c\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/strong><\/a><\/span>\u3002<\/strong><\/div>\n
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\u7d30\u80de\u6d3b\u6027\u5448\u8272\u5206\u6790\u5957\u7d44 | \u76f8\u95dc\u5206\u6790<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0K306-200<\/td>\n\u00a0BrdU Cell Proliferation Assay Kit<\/a><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K306-1000<\/td>\n\u00a0BrdU Cell Proliferation Assay Kit<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K307-1000<\/td>\n\u00a0Cell Proliferation Assay Kit (Fluorometric)<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K4194-100<\/td>\n\u00a0CXCR4 (Human) ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K305-1000<\/td>\n\u00a0EZViable\u2122 Calcein AM Cell Viability Assay Kit (Fluorometric)<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K300-500<\/td>\n\u00a0MTS Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K300-2500<\/td>\n\u00a0MTS Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K299-1000<\/td>\n\u00a0MTT Cell Proliferation Assay Kit (Colorimetric)<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K4249-100<\/td>\n\u00a0Phospho-P70S6K (Thr444\/Ser447) ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4193-100<\/td>\n\u00a0PTEN (Human) ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K303-2500<\/td>\n\u00a0VisionBlue\u2122 Quick Cell Viability Fluorometric Assay Kit<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K303-500<\/td>\n\u00a0VisionBlue\u2122 Quick Cell Viability Fluorometric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K302-2500<\/td>\n\u00a0WST Cell Proliferation Colorimetric Assay Kit plus<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K302-500<\/td>\n\u00a0WST Cell Proliferation Colorimetric Assay Kit plus<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K301-2500<\/td>\n\u00a0WST-1 Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K301-500<\/td>\n\u00a0WST-1 Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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K301 \u5f15\u7528\u6587\u737b<\/strong><\/div>\n
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Hsueh, Yu-Sheng et al. (2017) Laminin-Alginate Beads as Preadipocyte Carriers to Enhance Adipogenesis In Vitro and In Vivo,\u00a0Tissue Eng Part A. 2017 Mar;23(5-6):185-194.<\/a><\/div>\n<\/div>\n
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Fang, Y. et al., (2017) \u00a0IL-33 acts as a foe to MIA PaCa-2 pancreatic cancer,\u00a0Medical Oncology, Feb.2017, 34(2), 23<\/a><\/div>\n<\/div>\n
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Cheng et al., Autophagy modulates endoplasmic reticulum stress-induced cell death in podocytes: A protective role.\u00a0Experimental Biology and Medicine,\u00a0Oct 2014; 10.1177\/1535370214553772.<\/a><\/div>\n<\/div>\n
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Murdoch et al., Glutaredoxin-1 Up-regulation Induces Soluble Vascular Endothelial Growth Factor Receptor 1, Attenuating Post-ischemia Limb Revascularization.\u00a0J. Biol. Chem., Mar 2014; 289: 8633 – 8644.<\/a><\/div>\n<\/div>\n
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Sefton et al., MK-2206, an AKT Inhibitor, Promotes Caspase-Independent Cell Death and Inhibits Leiomyoma Growth.Endocrinology,\u00a0 Nov 2013; 154: 4046 – 4057.<\/a><\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Cell Proliferation Assay Kits | \u8ca8\u865fK301-500 \uff08abcam ab654 […]<\/p>\n","protected":false},"author":3,"featured_media":7922,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[180],"tags":[4151],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7921"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7921"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7921\/revisions"}],"predecessor-version":[{"id":11698,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7921\/revisions\/11698"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7922"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7921"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7921"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}