{"id":7829,"date":"2016-11-22T16:13:43","date_gmt":"2016-11-22T08:13:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7829"},"modified":"2022-07-25T11:32:22","modified_gmt":"2022-07-25T03:32:22","slug":"percpcy5-5%e6%8a%97%e9%ab%94%e8%9b%8b%e7%99%bd%e8%b3%aa%e6%a8%99%e8%aa%8c%e5%a5%97%e7%b5%84-innova-biosciences-%e8%b2%a8%e8%99%9f763-0030","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7829","title":{"rendered":"PerCP\/Cy5.5\u6297\u9ad4\u86cb\u767d\u8cea\u6a19\u8a8c\u5957\u7d44 |INNOVA Biosciences \u8ca8\u865f#763-0030"},"content":{"rendered":"

FRET \u87a2\u5149\u6280\u8853!! \u00a0Lightning-Link\u00ae PerCP\/Cy5.5<\/strong>\u00a0| <\/strong>\u6297\u9ad4<\/strong>\u86cb\u767d\u8cea<\/strong>\u6a19\u8a8c\u5957\u7d44<\/strong><\/span> \u8ca8\u865f<\/strong>#<\/strong>763<\/strong>–<\/strong>0030<\/strong>\u5305\u88dd<\/strong>3x10UG<\/strong><\/span><\/p>\n

\u53ea\u9700\u4e00\u500b\u6b65\u9a5f\u4e0d\u9808Dialysis\u6216desalt\u6b65\u9a5f\u3002\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u8fd1\u4e4e100%\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u985e\u5225:PerCP\/Cy5.5\u87a2\u5149\u86cb\u767d \u6297\u9ad4\/\u86cb\u767d\u8cea\/\u80dc\u80bd\uff08amino group\uff09\u6a19\u8a8c<\/strong><\/p>\n

\u7522\u54c1\u654d\u8ff0:PerCP-Cy5.5<\/strong>\u87a2\u5149dye\u6297\u9ad4\u6a19\u8a8cdirectly labeling kit (\u8ca8\u865f#763-0030)\uff0c\u9996\u9078\u82f1\u570binnova biosciences\u516c\u53f8\u5c08\u5229\u7814\u767c\u4e4blightning-link\u6297\u9ad4\u87a2\u5149\u6a19\u8a8c\u5957\u7d44\u3002\u5e02\u9762\u4e0aantibody labeling kit\uff0c\u5728pH8~9\u74b0\u5883\u4e0b\u505a\u53cd\u61c9\u3002\u5982\u6b64\u5730\u74b0\u5883\u6613\u9020\u6210\u6297\u9ad4\u6d3b\u6027\u5931\u6548\uff0c\u5373\u4f7f\u7167\u8457\u539f\u5ee0\u63d0\u4f9b\u7684Protocol\u4e0b\u9032\u884c\u53cd\u61c9\uff0c\u6297\u9ad4\u56de\u6536\u7684\u6548\u80fd\u4e5f\u5f88\u5dee; \u592a\u591a\u6297\u9ad4\u672a\u88ab\u6a19\u8a8c\uff0c\u6240\u4ee5\u5fc5\u9808\u7d93\u7531Dialysis\u53cadesalt\u6b65\u9a5f\u79fb\u53bb\u672a\u7d50\u5408\u7684\u6297\u9ad4\u3002\u5982\u6b64\u4e00\u4f86\u6d6a\u8cbb\u4e86\u73cd\u8cb4\u7684\u6297\u9ad4, \u6297\u9ad4\u7d50\u5408\u6548\u80fd\u4e5f\u4e0d\u7406\u60f3\u3002Lightning-link\u5546\u54c1\u514b\u670d\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u4e0d\u7406\u60f3\u7684\u554f\u984c\uff0c\u5c08\u5229\u7814\u767c\u6539\u826f\uff0c\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u8fd1\u4e4e100%\uff0c\u662f\u6a19\u8a8c\u7684\u9996\u9078\u5546\u54c1\u3002\u6a19\u8a8c\u6297\u9ad4\u7bc4\u570d10ug-1mg\uff0c\u5927\u91cf\u6a19\u8a8c\u8acb\u4f86\u96fb\u6d3d\u8a6202-86609496<\/a><\/p>\n

\u751f\u7269\u6a19\u8a8c\u7684\u5c08\u5bb6<\/strong>innova<\/strong> biosciences<\/strong><\/p>\n

\u82f1\u570bINNOVA BIOSCIENCES\uff0c\u4ee5\u751f\u7269\u7d50\u5408\u70ba\u4e3b\u8981\u4e8b\u696d\u3002\u65bc1996\u5e74\u5c08\u5229\u7814\u767c\uff0c\u4e2d\u6027\u74b0\u5883\u4e0b\u9032\u884c\u6297\u9ad4\u7d50\u5408\u53cd\u61c9\uff0c\u53ea\u8981\u4e00\u500b\u6b65\u9a5f\uff0c30\u79d2hands on time\u3002\u5176\u6297\u9ad4\u56de\u6536\u6548\u80fd\u8fd1\u4f3c100%\u3002\u4e0d\u9808\u518d\u505aDialysis\u6216desalt\u7684\u6b65\u9a5f\u3002Lightning-Link \u53ea\u8981\u4e00\u500b\u6b65\u9a5f\uff0c\u5373\u53ef\u5c07\u86cb\u767d\u8cea\/\u6297\u9ad4\/\u80dc\u80bd\u505a\u6a19\u8a8c\uff0c\u5177\u5c08\u5229\u7814\u767cQuencher reagent\u5316\u5b78\u53cd\u61c9\u5fb9\u5e95\u4e2d\u6b62\u6b98\u9918\u53cd\u61c9\u53ca\u505c\u6b62\u975e\u5c08\u4e00\u6027\u7d50\u5408\uff0c\u56e0\u6b64\u7121\u9808desalt\u53cadialysis\uff0c\u6297\u9ad4\u6a19\u8a8c\u56de\u6536\u7387100%\u3002<\/p>\n

Lightning-link<\/strong>\u6297\u9ad4\u6a19\u8a8c\u539f\u7406<\/strong><\/p>\n

Lightning-Link\u4ee5\u5171\u50f9\u9375\u7d50\uff0c\u8fa8\u8996amino groups\uff0c\u6027\u8cea\u6975\u70ba\u7a69\u5b9a\uff0c\u4e00\u6b21\u53cd\u61c9\u5f8c\uff0c\u53ef\u51b7\u51cd\u4fdd\u5b58\uff0c\u7bc0\u7701\u5be6\u9a57\u6642\u9593\u3002<\/p>\n

\"\"<\/p>\n

PerCP\/Cy5.5\u87a2\u5149Dye\u5149\u8b5c<\/strong>\u00a0484\/692(excitation\/emission)<\/strong><\/p>\n

PerCP\/Cy5.5 is a tandem label. In the Lightning Link PerCP\/Cy5.5 tandem when the PerCP is excited it functions as a donor and the energy that would be released as PerCP fluorescence is transferred via FRET (F\u00f6rster Resonance Energy Transfer) to the acceptor Cy5.5. Cy5.5 then emits the energy as red fluorescence.<\/p>\n

Lightning-link<\/strong>\u6297\u9ad4\u6a19\u8a8c\u61c9\u7528<\/strong><\/p>\n

\u53ef\u4ee5\u61c9\u7528\u5728ELISA. immunofluorescent. Flow. confocol. Western Blot\u7b49\u6aa2\u8a3a\u53ca\u5206\u5b50\u75c5\u6bd2\u4e4b\u7814\u7a76!!\u76f4\u63a5\u5728\u4e00\u6297\u4e0a\u505a\u6a19\u8a8c (\u53ef\u6a19\u8a8c\u87a2\u5149\u86cb\u767d \u87a2\u5149dye HRP Biotin\u7b49) \uff0c\u514b\u670d\u4e8c\u6297\u6240\u9020\u6210\u7684\u80cc\u666f\u503c\u904e\u9ad8\u53caHost\u4f86\u6e90\u4e0d\u540c\u6240\u9020\u6210Crosstalk\u7684\u73fe\u8c61\u3002Lightning-link\u6297\u9ad4\u6a19\u8a8cV.S.\u4fdd\u5b58\u6db2\u00a0<\/strong>\u6297\u9ad4\u4fdd\u5b58\u65bcAzide\u3001Gelatin\u53caBSA\u6eb6\u6db2\u4e0b\uff0c\u6703\u5e72\u64fe\u4e00\u822c\u7d50\u5408\u7684\u6548\u679c\uff0c\u6240\u4ee5\u6703\u518d\u7d93\u904e\u7d14\u5316\u6b65\u9a5f\uff0c\u6d6a\u8cbb\u8a31\u591a\u6297\u9ad4\u3002Lightning-Link\u514b\u670dAzide\u3001Gelatin\u53caBSA\u5e72\u64fe\u7d50\u5408\u7684\u6548\u7387\uff0c\u7701\u4e0b\u60a8\u5bf6\u8cb4\u7684\u6297\u9ad4\u3002\u5225\u65bc\u5176\u5b83\u7d50\u5408\u7684\u65b9\u5f0f\uff0cLightning-Link\u7121\u9808\u5728High pH\u7684\u6eb6\u6db2\u4e0b\u64cd\u4f5c\uff0c\u6240\u4ee5\u53ea\u8981\u662f\u5177\u6709amino groups (lysin\/a-amino)\u7684\u86cb\u767d\u8cea\/\u80dc\u80bd\/\u6297\u9ad4\uff0c\u90fd\u53ef\u53cd\u61c9\u3002<\/p>\n

\u66f4\u591a\u6297\u9ad4\u6a19\u8a8c\u76f8\u95dc<\/span><\/strong>\u00a0 InnovaGold\u5948\u7c73\u91d1\u6a19\u8a8c\u5feb\u7be9\u8a66\u5291<\/a> | Lighting-link\u9175\u7d20\u578b\u6297\u9ad4\u6a19\u8a8c<\/a> | Abselect\u6297\u9ad4\u7d14\u5316 <\/a>| SBA\u55ae\u682a\u6297\u9ad4\u5206\u578bELISA<\/a> | Lightning-link\u87a2\u5149\u86cb\u767d\u6297\u9ad4\u6a19\u8a8c<\/a> | Thunder-link\u6838\u9178\u6297\u9ad4\u6a19\u8a8c<\/a><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Lightning-Link\u00ae*<\/strong><\/td>\n<\/td>\n\u00a0Lightning-Link\u00ae Rapid*<\/td>\nMaximal Absorbance <\/strong>
\n(nm)<\/strong><\/td>\n		Maximal Emission <\/strong>
\n(nm)<\/strong><\/td>\n<\/tr>\n
AMCA<\/td>\n<\/td>\n313-0030<\/a><\/td>\n352<\/td>\n452<\/td>\n<\/tr>\n
DyLight\u00ae 350<\/td>\n<\/td>\n\u00a0320-0030<\/a><\/td>\n354<\/td>\n432<\/td>\n<\/tr>\n
Atto 390<\/td>\n<\/td>\n349-0030<\/a><\/td>\n388<\/td>\n468<\/td>\n<\/tr>\n
DyLight\u00ae 405<\/td>\n<\/td>\n321-0030<\/a><\/td>\n402<\/td>\n428<\/td>\n<\/tr>\n
PerCP<\/td>\n718-0030<\/a><\/td>\n<\/td>\n484<\/td>\n678<\/td>\n<\/tr>\n
PerCP\/Cy5.5<\/td>\n763-0030<\/a><\/td>\n<\/td>\n484<\/td>\n692<\/td>\n<\/tr>\n
DyLight\u00ae 488<\/td>\n\u00a0<\/a><\/td>\n322-0030<\/a><\/td>\n496<\/td>\n524<\/td>\n<\/tr>\n
Fluorescein<\/td>\n707-0030<\/a><\/td>\n310-0030<\/a><\/td>\n498<\/td>\n532<\/td>\n<\/tr>\n
R-Phycoerythrin<\/td>\n703-0030<\/a><\/td>\n\u00a0<\/a><\/td>\n498,544,566**<\/td>\n580<\/td>\n<\/tr>\n
PE\/Texas Red<\/td>\n767-0030<\/a><\/td>\n<\/td>\n498,544,566**<\/td>\n618<\/td>\n<\/tr>\n
PE\/Atto594<\/td>\n768-0030<\/a><\/td>\n<\/td>\n498,544,566**<\/td>\n632<\/td>\n<\/tr>\n
PE\/Cy5<\/td>\n760-0030<\/a><\/td>\n<\/td>\n498,544,566**<\/td>\n672<\/td>\n<\/tr>\n
PE\/Cy5.5<\/td>\n761-0030<\/a><\/td>\n<\/td>\n498,544,566**<\/td>\n700<\/td>\n<\/tr>\n
PE\/Cy7<\/td>\n762-0030<\/a><\/td>\n<\/td>\n498,544,566**<\/td>\n782<\/td>\n<\/tr>\n
Atto 488<\/td>\n\u00a0<\/a><\/td>\n350-0030<\/a><\/td>\n504<\/td>\n530<\/td>\n<\/tr>\n
B-Phycoerythrin<\/td>\n716-0005<\/a><\/td>\n\u00a0<\/a><\/td>\n546<\/td>\n580<\/td>\n<\/tr>\n
Cyanine Dye 3 (Cy3)<\/td>\n<\/td>\n340-0030<\/a><\/td>\n552<\/td>\n576<\/td>\n<\/tr>\n
Rhodamine<\/td>\n\u00a0<\/a><\/td>\n311-0030<\/a><\/td>\n555<\/td>\n588<\/td>\n<\/tr>\n
DyLight\u00ae 550<\/td>\n<\/td>\n323-0030<\/a><\/td>\n556<\/td>\n584<\/td>\n<\/tr>\n
Atto 565<\/td>\n<\/td>\n351-0030<\/a><\/td>\n570<\/td>\n598<\/td>\n<\/tr>\n
DyLight\u00ae 594<\/td>\n<\/td>\n324-0030<\/a><\/td>\n594<\/td>\n629<\/td>\n<\/tr>\n
Texas Red\u00ae<\/td>\n<\/td>\n315-0030<\/a><\/td>\n596<\/td>\n616<\/td>\n<\/tr>\n
DyLight\u00ae 633<\/td>\n<\/td>\n325-0030<\/a><\/td>\n628<\/td>\n660<\/td>\n<\/tr>\n
Atto 633<\/td>\n<\/td>\n353-0030<\/a><\/td>\n634<\/td>\n660<\/td>\n<\/tr>\n
FluoProbes647H<\/td>\n<\/td>\n362-0030<\/a><\/td>\n650<\/td>\n684<\/td>\n<\/tr>\n
Cyanine Dye 5 (Cy5)<\/td>\n<\/td>\n342-0030<\/a><\/td>\n652<\/td>\n678<\/td>\n<\/tr>\n
Allophycocyanin<\/td>\n705-0005<\/a><\/td>\n<\/td>\n652<\/td>\n666<\/td>\n<\/tr>\n
APC\/Cy5.5<\/td>\n764-0030<\/a><\/td>\n<\/td>\n652<\/td>\n700<\/td>\n<\/tr>\n
APC\/Cy7<\/td>\n765-0030<\/a><\/td>\n<\/td>\n652<\/td>\n790<\/td>\n<\/tr>\n
DyLight\u00ae 650<\/td>\n\u00a0<\/a><\/td>\n326-0030<\/a><\/td>\n656<\/td>\n686<\/td>\n<\/tr>\n
Cyanine Dye 5.5 (Cy5.5)<\/td>\n<\/td>\n343-0030<\/a><\/td>\n680<\/td>\n705<\/td>\n<\/tr>\n
DyLight\u00ae 680<\/td>\n<\/td>\n327-0030<\/a><\/td>\n686<\/td>\n716<\/td>\n<\/tr>\n
Atto 700<\/td>\n<\/td>\n354-0030<\/a><\/td>\n704<\/td>\n724<\/td>\n<\/tr>\n
DyLight\u00ae 755<\/td>\n<\/td>\n328-0030<\/a><\/td>\n756<\/td>\n794<\/td>\n<\/tr>\n
DyLight\u00ae 800<\/td>\n<\/td>\n329-0030<\/a><\/td>\n776<\/td>\n798<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

FRET \u87a2\u5149\u6280\u8853!! \u00a0Lightning-Link\u00ae PerCP\/Cy5.5\u00a0| \u6297\u9ad4\u86cb\u767d\u8cea\u6a19\u8a8c\u5957\u7d44 \u8ca8\u865f […]<\/p>\n","protected":false},"author":3,"featured_media":6804,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[195],"tags":[4169],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7829"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7829"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7829\/revisions"}],"predecessor-version":[{"id":7868,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7829\/revisions\/7868"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6804"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7829"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7829"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7829"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}