{"id":779,"date":"2016-08-07T16:39:52","date_gmt":"2016-08-07T08:39:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=779"},"modified":"2022-07-27T10:57:49","modified_gmt":"2022-07-27T02:57:49","slug":"blocking-buffers","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=779","title":{"rendered":"Protein free Blocking \u7de9\u885d\u6db2 | \u812b\u8102\u725b\u5976\u6216BSA\u6eb6\u6db2\uff0c\u963b\u65b7\u975e\u7279\u7570\u6027\u6297\u539f\u7d50\u5408\u4f4d"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"line-height: 1.5em;\">\u3000\u3000\u50b3\u7d71\u4e0a\u4f7f\u7528\u812b\u8102\u725b\u5976\u6216BSA\u6eb6\u6db2\uff0c\u963b\u65b7\u975e\u7279\u7570\u6027\u6297\u539f\u7d50\u5408\u4f4d\u3002\u5e38\u61c9\u7528\u65bcWB\u3001ELISA\u3001IHC\u7b49\u5206\u6790\u3002<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: medium; color: #008000;\"><strong>\u7522\u54c1\u7a2e\u985e<\/strong><\/span><\/p>\n<ul>\n<li><strong style=\"line-height: 1.5em;\">Protein Free Blocking Buffer\uff1a<\/strong><span style=\"line-height: 1.5em;\">\u4e0d\u542b\u86cb\u767d\u6210\u4efd\uff0c\u9069\u7528\u65bc\u80cc\u666f\u8f03\u9ad8\u7684sample or blotting\u3002<\/span>\n<ul>\n<li>\u7528\u9014\uff1aWestern blot\u3001ELISA\u3001Protein array\u7b49\u5be6\u9a57\u3002<\/li>\n<li>\u6240\u9700\u6642\u9593\uff1a15-30min\u3002<\/li>\n<\/ul>\n<\/li>\n<li><strong style=\"line-height: 1.5em;\">Fast Blocking Buffer\uff1a<\/strong><span style=\"line-height: 1.5em;\">\u6240\u9700\u6642\u9593\u77ed\u3002\u53ef\u505a\u70ba\u6297\u9ad4\u914d\u7f6e\u6db2\uff0c\u5927\u5e45\u6e1b\u5c11blocking\u6642\u9593\u3002<\/span>\n<ul>\n<li>\u7528\u9014\uff1aWestern blot\u3002<\/li>\n<li>\u6240\u9700\u6642\u9593\uff1a5-10min\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><span style=\"font-size: medium; color: #008000;\"><b>\u4f7f\u7528\u65b9\u6cd5<\/b><\/span><\/p>\n<ol>\n<ol>\n<li>\u53d6\u51faBlotting membrane\uff0c\u5c07transfer buffer \u6d17\u4e7e\u6de8\u3002<\/li>\n<li>\u52a0\u5165Blocking buffer\uff0c\u6df9\u904emembrane\u5f8c\u5747\u52fb\u6416\u64fa\u6df7\u5408\u3002<\/li>\n<li>\u52a0\u5165\u914d\u88fd\u597d\u7684\u6297\u9ad4\u6db2\uff0c\u9032\u884c\u5f8c\u7e8c\u6b65\u9a5f\u3002<\/li>\n<\/ol>\n<\/ol>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\">\n<tbody>\n<tr>\n<td width=\"100\">\n<p align=\"center\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n<td width=\"476\">\n<p align=\"center\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/p>\n<\/td>\n<td width=\"100\">\n<p align=\"center\"><strong>\u5305\u88dd<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">JK92-W001<\/td>\n<td valign=\"top\" width=\"476\">Fast Blocking Buffer (in PBS)<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">500ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">JK92-W002<\/td>\n<td valign=\"top\" width=\"476\">Fast Blocking Buffer (in TBS)<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">500ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">JK92-W003<\/td>\n<td valign=\"top\" width=\"476\">Protein Free Blocking Buffer (in PBS)<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">500ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">JK92-W004<\/td>\n<td valign=\"top\" width=\"476\">Protein Free Blocking Buffer (in TBS)<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">500ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000\u50b3\u7d71\u4e0a\u4f7f\u7528\u812b\u8102\u725b\u5976\u6216BSA\u6eb6\u6db2\uff0c\u963b\u65b7\u975e\u7279\u7570\u6027\u6297\u539f\u7d50\u5408\u4f4d\u3002\u5e38\u61c9\u7528\u65bcWB\u3001ELISA\u3001IHC\u7b49\u5206\u6790\u3002 \u7522\u54c1\u7a2e\u985e [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":783,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89,135,3],"tags":[4678],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/779"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=779"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/779\/revisions"}],"predecessor-version":[{"id":7385,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/779\/revisions\/7385"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/783"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=779"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=779"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=779"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}