{"id":7782,"date":"2016-11-18T13:49:32","date_gmt":"2016-11-18T05:49:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7782"},"modified":"2022-07-25T11:34:15","modified_gmt":"2022-07-25T03:34:15","slug":"bsa%e7%a7%bb%e9%99%a4%e8%a9%a6%e5%8a%91%e7%9b%92-abselect-bsa-removal-kit-%e8%b2%a8%e8%99%9f820-0100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7782","title":{"rendered":"BSA\u79fb\u9664\u8a66\u5291\u76d2 |AbSelect BSA Removal kit \u8ca8\u865f820-0100"},"content":{"rendered":"

BSA<\/strong>\u79fb\u9664\u8a66\u5291\u76d2\u00a0| \u6297\u9ad4\u6fc3\u7e2e\u53ca\u7d14\u5316\u7cfb\u5217\u5546\u54c1 <\/strong>\u00a0(\u4ea6\u53ef\u7528\u65bcGlycine\u53caTris \u79fb\u9664)\u00a0<\/a><\/span><\/strong><\/span>AbSelect BSA Removal\u00a0kit \u8ca8\u865f820-0100<\/a><\/span><\/p>\n

Innova Bioseiences\u63d0\u4f9b\u4e00\u7cfb\u7d71\u6297\u9ad4\u7d14\u5316\u5957\u7d44\uff0c\u6bcf\u7d44\u5957\u7d44\u5305\u62ec\u6297\u9ad4\u7d14\u5316\u4e4bbuffer\u53caresin\uff0c\u6709\u6548\u7d14\u5316\u4e0d\u540c\u4f86\u6e90\u4e4b\u6297\u9ad4\u542b\u91cf (\u00a0\u6297\u9ad4\u6fc3\u5ea60.03mg\/ml ~ 10mg\/ml, \u6bcf\u6b21\u53cd\u61c9\u9ad4\u7a4d100ul) \u3002\u5176\u4e2dAbSelect BSA\u53bb\u9664\u8a66\u5291\u76d2\u7c21\u55ae\u4e00\u500b\u6b65\u9a5f\uff0c10\u5206\u9418\u65b9\u6cd5\uff0c\u6709\u6548\u5730\u5f9e\u6297\u9ad4\u5206\u96e2BSA\uff0c\u53ef\u4ee5\u7528\u65bc\u4efb\u4f55\u6297\u9ad4subtype\u6216\u4e0d\u540c\u4e4b\u7269\u7a2e\u3002AbSelect BSA\u53bb\u9664\u8a66\u5291\u76d2\u7684\u512a\u9ede\uff1b\u4f7f\u7528\u65b9\u4fbf\u53ea\u9700\u898110\u5206\u9418\uff1b\u96e2\u5fc3\u6a5f\u662f\u552f\u4e00\u9700\u8981\u7684\u8a2d\u5099\uff0c\u53ef\u7528\u65bc\u6fc3\u7e2e\u548cBuffer exchange\u4e4b\u529f\u80fd\u3002<\/p>\n

\u7522\u54c1\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805: 100\u00b5l\u00a0 \u6297\u9ad4 (BSA concentration is 0.5%) , \u52a0\u5165 80\u00b5l of the AbSelectTM BSA Removal Buffer \u53cd\u61c9\u3002\u82e5\u6297\u9ad4\u91cf\u4f4e\u65bc50\u00b5g, lower limit for seeing a clearly visible pellet.<\/p>\n

\"alt\"<\/p>\n

\u6297\u9ad4\u4fdd\u5b58\u6db2\u4e2d\uff0c\u901a\u5e38\u9ad8\u6fc3\u5ea6\u4e4b\u86cb\u767d\u8cea\uff0c\u4f8b\u5982\u725b\u8840\u6e05\u767d\u86cb\u767d\uff08BSA\uff09\u5230\u7d14\u5316\u7684\u6297\u9ad4\u4e2d\uff0c\u505a\u70ba\u6709\u6548\u7684\u6297\u9ad4\u7a69\u5b9a\u5291\u3002\u7136\u800c\uff0c\u7576\u6297\u9ad4\u9810\u9032\u884c\u4e0b\u6e90\u6a19\u8a8c\u5be6\u9a57\u6642\uff0cBSA\u8b8a\u6210\u963b\u7919\uff0c\u6703\u76f4\u63a5\u8207\u6297\u9ad4\u7af6\u722d\u9644\u8457\u5230\u6a19\u8a18\u4e0a\uff0c\u5e72\u64fe\u9175\u7d20\u578bHRP\u3001Biotin\u3001streptavidin\u53caFITC\u87a2\u5149\u7b49\u4e4b\u6297\u9ad4\u6a19\u8a8c\u53cd\u61c9\uff0c\u56e0\u6b64\u964d\u4f4e\u4e86\u6297\u9ad4\u6a19\u8a8c\u7684\u6548\u7387\u3002\u5728\u9032\u884c\u6a19\u8a18\u6280\u8853\u4e4b\u5be6\u9a57\u524d\uff0c\u9032\u4e00\u6b65\u79fb\u9664\u6eb6\u6db2\u4e2d\u6b98\u7559\u4e4bamino group\u3002\u5e38\u898b\u7684\u5546\u696dBSA\u53bb\u9664\u6280\u8853\u53ef\u80fd\u6d89\u53ca\u8a31\u591a\u8cbb\u529b\u7684\u6b65\u9a5f\u3002 AbSelect BSA\u53bb\u9664\u8a66\u5291\u76d2\u7c21\u55ae\u7684\u4e00\u500b\u6b65\u9a5f\uff0c10\u5206\u9418\u65b9\u6cd5\uff0c\u6709\u6548\u5730\u5f9e\u6297\u9ad4\u5206\u96e2BSA\uff0c\u53ef\u4ee5\u7528\u65bc\u4efb\u4f55\u6297\u9ad4subtype\u6216\u4e0d\u540c\u4e4b\u7269\u7a2e\u3002<\/p>\n

\u200b<\/span>\u200b<\/span><\/span><\/span><\/span>DIY<\/strong>\u6297\u9ad4\u6a19\u8a8c\u87a2\u5149<\/strong>(FITC,PE,Cy3,Cy5,Cy7 dye)<\/strong>\u6ce8\u610f\u4e8b\u9805<\/p>\n

\u8acb\u78ba\u8a8d\u6297\u9ad4\u7684\u4fdd\u5b58\u689d\u4ef6\u3002\u50b3\u7d71\u5e02\u552e\u7684\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44\uff0c\u5728\u4f7f\u7528\u524d\u7686\u9700dialysis\u7d14\u5316\uff0c\u5c07\u6297\u9ad4\u4fdd\u5b58\u7684\u7269\u8cea\u79fb\u9664\u3002Lightning-link\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44\u5728\u5927\u90e8\u4efd\u7684\u4fdd\u5b58\u689d\u4ef6\u4e0b\uff0c\u4e0d\u9700\u8981\u518ddialysis\u7684\u6b65\u9a5f\uff0c\u4f8b\u5982\u4e00\u822csalts (e.g. NaCl)\u3001sugars(e.g. sucrose)\u3001\u53cachelators (e.g. EDTA)\u4e0d\u6703\u5f71\u97ff\u6a19\u8a8c\u7684\u6d3b\u6027\u3002\u5927\u90e8\u7684\u5e02\u552e\u7684\u6297\u9ad4\u4fdd\u5b58\u57281%BSA\u30010.5%Azide\u53ca0.5% Gelatin \u8f15\u5fae\u6216\u5e7e\u4e4e\u4e0d\u6703\u5f71\u97ff\u6a19\u8a8c\u7684\u6d3b\u6027\u3002\u5728\u5c11\u6578\u60c5\u6cc1\u4e0b\uff0c\u82e5\u662f\u60a8\u8cfc\u8cb7\u7684\u6297\u9ad4\u4fdd\u5b58\u5728amino acids(e.g. glycine)\u3001blockers(e.g. ethanolamine)\u53cathiols(DTT, mercaptoethanol)\u60c5\u6cc1\u4e0b\uff0c\u8a18\u5f97\u7d14\u5316\u60a8\u7684\u6297\u9ad4\uff0c\u4f7f\u7528abselect\u00a0\u6297\u9ad4\u7d14\u5316\u7cfb\u5217\u5546\u54c1\uff0c\u907f\u514d\u6291\u5236\u6a19\u8a8c\u6d3b\u6027!<\/p>\n

\u6297\u9ad4\u7d14\u5316\u76f8\u95dc\u5546\u54c1<\/span><\/strong>\u00a0TCS\u6297\u9ad4\u7d14\u5316\u7cfb\u7d71 \uff08Mouse IgG\u00a0\uff09<\/a>\u00a0|\u00a0BSA Removal\u00a0kit\u00a0<\/a>\u00a0|\u00a0\u6297\u9ad4\u7d14\u5316 (\u8179\u6c34\/\u514d\u75ab\u8840\u6e05 )\u00a0<\/a>\u00a0|\u00a0\u6297\u9ad4\u6fc3\u7e2e\u548cClean-up\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n\u5546\u54c1\u5167\u5bb9<\/td>\n<\/tr>\n
(861-0010)<\/td>\nAbSelect Antibody Concentration and Clean-Up Kit <\/a><\/td>\n<\/tr>\n
(842-0005)<\/td>\nAbSelect Rat TCS Purification System <\/a><\/td>\n<\/tr>\n
(820-0100)<\/td>\nAbSelect BSA Removal Kit <\/a><\/td>\n<\/tr>\n
(830-0010)<\/td>\nAbSelect Mouse Antibody Purification System <\/a><\/td>\n<\/tr>\n
(860-0010)<\/td>\nAbSelect Antibody Purification System <\/a><\/td>\n<\/tr>\n
(890-0010)<\/td>\nAbSelect G Antibody Purification System <\/a><\/td>\n<\/tr>\n
(832-0500)<\/td>\nAbSelect Mouse TCS Purification System <\/a><\/td>\n<\/tr>\n
(862-0500)<\/td>\nAbSelect TCS Antibody Purification System <\/a><\/td>\n<\/tr>\n
(863-0500)<\/td>\nAbSelect Serum Antibody Purification System <\/a><\/td>\n<\/tr>\n
(893-0500)<\/td>\nAbSelect G Serum Antibody Purification System <\/a><\/td>\n<\/tr>\n
(851-0024)<\/td>\nProtein A Agarose <\/a><\/td>\n<\/tr>\n
(895-0024)<\/td>\nProtein G Agarose <\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

BSA\u79fb\u9664\u8a66\u5291\u76d2\u00a0| \u6297\u9ad4\u6fc3\u7e2e\u53ca\u7d14\u5316\u7cfb\u5217\u5546\u54c1 \u00a0(\u4ea6\u53ef\u7528\u65bcGlycine\u53caTris \u79fb\u9664)\u00a0AbSelect […]<\/p>\n","protected":false},"author":3,"featured_media":7786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[151],"tags":[4182],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7782"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7782"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7782\/revisions"}],"predecessor-version":[{"id":21540,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7782\/revisions\/21540"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7786"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7782"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7782"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7782"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}