{"id":7778,"date":"2016-11-18T10:47:03","date_gmt":"2016-11-18T02:47:03","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7778"},"modified":"2023-04-24T12:45:53","modified_gmt":"2023-04-24T04:45:53","slug":"atpase%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-innova-biosciences%e8%b2%a8%e8%99%9f601-0120-%e5%90%abpicolorlock%ef%bc%89","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7778","title":{"rendered":"ATPase\u5206\u6790\u5957\u7d44 |Innova biosciences\u8ca8\u865f601-0120 (\u542bPiColorLock\uff09 (abcam ab270551)"},"content":{"rendered":"

Colorimetric ATPase\u5206\u6790\u5957\u7d44<\/span>\u00a0|\u8ca8\u865f601-0120\uff08\u542bPiColorLock\uff09 (abcam\u00a0ab270551)\u00a0<\/strong><\/a><\/p>\n

601-0120 High Throughput Colorimetric ATPase Assays<\/span><\/strong><\/span><\/p>\n

 <\/p>\n

\"\"<\/p>\n

\"picolorlock_web\"<\/p>\n

Innova Biosciences \u63d0\u4f9b\u4e4bATPase\u5206\u6790\u5957\u7d44\uff0c\u53ef\u63a2\u8a0e\u9175\u7d20\u6d3b\u6027\u662f\u5426\u626e\u6f14transport\u3001signal transduction\u3001protein biosynthesis\u53caCell differentiation\u4e4b\u89d2\u8272\u3002\u61c9\u7528\u65bcATPase\u6d3b\u6027\u5206\u6790\uff0c\u63d0\u4f9bhigh-throughput screen \u5927\u91cfATPase inhibitor\u3002\u53ef\u5efa\u7acb\u5feb\u901f\u85e5\u7269\u7be9\u9078\u7cfb\u7d71\u3002\u63a1\u7528PiColorLock\u2122<\/strong><\/span>\u8d85\u9ad8\u9748\u654f\u5ea6Malachite green\u78f7\u9178\u6839\u5448\u8272\u65b9\u6cd5\uff0c\u5206\u6790ATPase\u9175\u7d20\u6d3b\u6027\uff0c\u514b\u670d\u50b3\u7d71\u5448\u8272\u65b9\u6cd5\u3001\u4f4e\u9748\u654f\u5ea6\u9650\u5236\u3002ATPase\u9175\u7d20\u5206\u6790\u5957\u7d44\uff0c\u5305\u62ecstabilizing solution\uff0c\u53ef\u907f\u958bacid labile substrate\u6240\u9020\u6210\u7684\u9ad8\u80cc\u666f\u503c\uff0c\u5176\u80cc\u666f\u503c\u5f88\u4f4e\uff081mM\uff09\u3002\u5176\u5206\u6790\u6ce2\u6bb5\u70ba600\uff0d660nm\uff0c\u907f\u958b\u4e00\u822ccolored compounds\u4e4b\u5e72\u64fe\u3002Pi-dye\u8907\u5408\u7269\u6975\u70ba\u7a69\u5b9a\uff0c\u5176\u8a0a\u865f\u5f37\u5ea6\u53ef\u7dad\u6301\u6578\u5c0f\u6642\u4e4b\u4e45\u3002\u9069\u7528\u65bc\u5927\u90e8\u4efd\u7684assay buffer\u3002\u86cb\u767d\u8cea\u6fc3\u5ea6\u9ad8\u6642\uff0c\u4e0d\u6703\u6291\u5236\u984f\u8272\u7684\u5f62\u6210\u3002<\/p>\n

PiColorLock\u2122<\/span><\/strong>\u78f7\u9178\u6839\u6aa2\u6e2c\u8a66\u5291\uff0c\u70ba\u6aa2\u6e2c\u7121\u6a5f\u78f7\u9178\u6839\uff08Pi\uff09\u4e4b\u542b\u91cf\uff1b\u4f7f\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\u3002\u53ef\u7528\u65bc\u6e2c\u91cf\u7522\u751fPi\u7684\u4efb\u4f55\u9176\uff0c\u5305\u62ecATPases\u3001GTPases, phosphatases\u3001\u71b1\u4f11\u514b\u86cb\u767d\u53caDNA\u89e3\u65cb\u86cb\u767d\u3002PiColorLock\u2122\u7684\u7368\u7279\u914d\u65b9\u63d0\u9ad8\u4e86\u6e2c\u5b9a\u7dda\u6027\uff0c\u52d5\u614b\u7bc4\u570d\u548c\u984f\u8272\u7a69\u5b9a\u6027\uff1b\u4e5f\u53ef\u4ee5\u7528PiColorLock\u8207\u4e0d\u7a69\u5b9a\u7684\u5e95\u7269\uff08\u4f8b\u5982ATP\uff0cGTP\uff09\u4e00\u8d77\u4f7f\u7528\uff0c\u6240\u8ff0\u5e95\u7269\u8207\u5176\u5b83\u9178\u6027\u67d3\u6599\u57fa\u6aa2\u6e2c\u8a66\u5291\u7522\u751f\u9ad8\u7684\u975e\u9176\u80cc\u666f\u4fe1\u865f\u3002\u6b64\u7522\u54c1\u4e0d\u5177\u653e\u5c04\u6027\u61c9\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\uff0c\u70ba\u9ad8\u9748\u654f\u7684\u78f7\u9178\u6839\u5075\u6e2c\u8a66\u5291\uff0c\u78ba\u4fdd\u7a69\u5b9a\u7684\u7da0\u8272dye-phosphate\u8907\u5408\u7269\u7522\u751f\uff0c\u907f\u514d\u767c\u751f\u53d7\u8cea\u975e\u9175\u7d20\u6c34\u89e3\u5e72\u64fe\u9020\u6210\u7684\u80cc\u666f\u96dc\u8a0a\u3002\u5075\u6e2c\u7bc4\u570d\u5ee3\uff0c\u53ef\u6e1b\u5c11\u6a23\u54c1\u7a00\u91cb\u7684\u5fc5\u8981\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Features<\/td>\nBenefits<\/td>\n<\/tr>\n
Colorimetric assay<\/td>\nSimple, scalable and non-radioactive<\/td>\n<\/tr>\n
Compatible with almost any assay buffer<\/td>\nFlexibility over a wide range of existing assays<\/td>\n<\/tr>\n
Stable reagent formulation<\/td>\nLong shelf life<\/td>\n<\/tr>\n
Unique accelerator<\/td>\nSpeeds up color development<\/td>\n<\/tr>\n
Unique stabilizer<\/td>\nSuppresses non enzymatic backgrounds with acid-labile substrates (ATP or GTP)<\/td>\n<\/tr>\n
PiColorLock\u2122-Pi complex is very stable<\/td>\nNo precipitation, results can be measured over several hours \u2013 ideal for HTS<\/td>\n<\/tr>\n
Wide linear range<\/td>\nNo inhibition of color development by high concentrations of protein<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\"<\/p>\n

Background stability<\/strong><\/span><\/div>\n
\n

ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock\u2122 provides baseline readings.<\/p>\n

\"\"<\/p>\n

Dynamic range<\/strong><\/span><\/div>\n
\n

Dynamic Range for ATPase Assay Kit (ab270551).<\/strong><\/p>\n

PiColorLockTM<\/sup>\u00a0has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors\u2019 products are linear over a much narrower range of concentrations.<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u578b\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
303-0125
\n303-0500<\/td>\n		PiColorLock Gold Phosphate Detection System\uff08\u9748\u654f\u5ea64\uff0d40uM\uff09<\/td>\n\u6700\u591a1560 assays
\n\u6700\u591a6250 assays<\/td>\n<\/tr>\n
601-0120
\n601-0121
\n601-0122<\/td>\n		High Throughput Colorimetric ATPase Assays<\/td>\n192 assays
\n480 assays
\n5 \u00d7 480 assays<\/td>\n<\/tr>\n
\u95dc\u806f\u5546\u54c1<\/strong><\/span><\/td>\n<\/tr>\n
601-9999<\/td>\n10mM Lyophilized ATP<\/td>\n0.5ml<\/td>\n<\/tr>\n
602-0120
\n602-0121
\n602-0122<\/td>\n		High Throughput Colorimetric GTPase Assays<\/td>\n192 assays
\n480 assays
\n5 \u00d7 480 assays<\/td>\n<\/tr>\n
501-0015<\/td>\nPiBind resin\uff0d\u5feb\u901f\u79fb\u9664\u6a23\u54c1\u4e2d\u6c61\u67d3\u7684\u78f7\u9178\u6839\uff08Pi\uff09<\/td>\n5g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Colorimetric ATPase\u5206\u6790\u5957\u7d44\u00a0|\u8ca8\u865f601-0120\uff08\u542bPiColorLock\uff09 (abca […]<\/p>\n","protected":false},"author":3,"featured_media":7780,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,181],"tags":[4183],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7778"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7778"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7778\/revisions"}],"predecessor-version":[{"id":41582,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7778\/revisions\/41582"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7780"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7778"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}