{"id":7727,"date":"2016-11-16T12:48:32","date_gmt":"2016-11-16T04:48:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7727"},"modified":"2022-07-25T11:35:31","modified_gmt":"2022-07-25T03:35:31","slug":"minute-%e7%b4%b0%e8%83%9e%e8%b3%aa%e8%88%87%e6%a0%b8%e8%9b%8b%e7%99%bd%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-cytoplasmic-nuclear-extraction-kit-%e8%b2%a8%e8%99%9fsm-003","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7727","title":{"rendered":"Minute\u2122 \u7d30\u80de\u8cea\u8207\u6838\u86cb\u767d\u8403\u53d6 |Cytoplasmic & Nuclear Extraction Kit invent\u8ca8\u865fSC-003"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>:<\/strong> Minute\u2122 \u00a0<\/strong>\u6838\u8cea\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/strong> | Cytoplasmic & Nuclear Extraction Kit <\/strong>\u8ca8\u865f<\/strong>SC-003<\/strong><\/p>\n

Minute\u2122\u7d30\u80de\u8cea\u8207\u6838\u86cb\u767d\u5206\u96e2\u8a66\u5291\u76d2 (Cytoplasmic & Nuclear protein extraction kit)\uff1b\u7121\u6d17\u6ecc\u5291\u548cEDTA\uff09\u7684\u6838\u8cea\u5206\u96e2\u8a66\u5291\u76d2\uff1b\u7d50\u5408\u7368\u7279\u7684\u5c08\u5229\u6280\u8853\u6a5f\u5236\uff0c\u7d30\u80de\/\u7d44\u7e54\u53ea\u9700\u901a\u904eFilter\u5373\u5b8c\u6210\u8cea\u819c\u86cb\u767d\u7684\u8403\u53d6\u3002\u5feb\u901f\u5206\u96e2\u5929\u7136\u7d30\u80de\u8cea\u548c\u6838\u86cb\u767d; \u53ef\u61c9\u7528\u65bc\u57f9\u990a\u7684\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u6216\u7d44\u7e54\u3002\u7531\u65bc\u4f7f\u7528\u86cb\u767d\u8cea\u63d0\u53d6\u6ffe\u7b52\uff0c\u53ef\u4ee5\u5728\u4e0d\u523015\u5206\u9418\u5167\u5b8c\u6210\u7d30\u80de\u8cea\u548c\u6838\u86cb\u767d\u7684\u5206\u96e2\u3002\u5728\u8a72\u904e\u7a0b\u4e2d\uff0c\u7d30\u80de\u819c\u7834\u88c2\u4e0d\u5fc5\u4f7f\u7528\u50b3\u7d71\u7684\u5747\u8cea\u5316\u6216\u8d85\u97f3\u6ce2\u8655\u7406\u3001\u4e0d\u9700\u8981\u8d85\u901f\u96e2\u5fc3\u3002\u8655\u7406\u91cf\uff1a1\u00d7106<\/sup>\uff0d50\u00d7106<\/sup> cells\/sample\u3002\u6642\u9593\uff1a\uff1c45 min\u3002Yield\uff1a1-3 mg\/ml\u3002\"Compined-F\"<\/strong><\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u7cfb\u5217\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300eColumn Filter\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00 \u5206\u9418<\/strong>\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981vortex\u3001transfer\u3001spin \u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

\"Cytoplasmic<\/p>\n

\n\n\n\n\n\n\n\n
Item<\/td>\nAmount<\/td>\nPart #<\/td>\n<\/tr>\n
Cytoplasmic Extraction Buffer<\/td>\n25 ml<\/td>\nSC-003-L<\/td>\n<\/tr>\n
Nuclear Extraction Buffer<\/td>\n25 ml<\/td>\nSC-003-N<\/td>\n<\/tr>\n
Protein Extraction Filter Cartridges<\/td>\n50<\/td>\nP-001<\/td>\n<\/tr>\n
Collection Tubes with Cap<\/td>\n50<\/td>\nP-002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

 <\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Fast Protein Extraction<\/strong><\/td>\n<\/tr>\n
Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
SD-001\/SN-002<\/a><\/td>\nTotal Protein Extraction Kit (for Animal Cultured Cells and Tissues)<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
SN-006<\/a><\/td>\nDetergent-Free Protein Extraction Kit (for Animal Cultured Cells and Tissues)<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
SD-008\/SN-009<\/a><\/td>\nTotal Protein Extraction Kit for Plant Tissues<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
SN-010<\/a><\/td>\nDetergent-Free Plant Protein Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
MS-026<\/a><\/td>\nTotal Protein Extraction Kit for Mass Spectrometry<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
AT-022<\/a><\/td>\nTotal Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n
SB-004<\/a><\/td>\nBacterial Total Protein Extraction Kits<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
YT-015<\/a><\/td>\nTotal Protein Extraction Kit for Microbes with Thick Cell Walls<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
YD-016<\/a><\/td>\nDetergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
HD-021<\/a><\/td>\nProtein Extraction Kit for Hair and Nails<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n
FE-025<\/a><\/td>\nProtein Extraction Kit for Fixed and Embedded Tissues<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sub-Cellular Structure Fractionation\/Isolation<\/strong><\/td>\n<\/tr>\n
Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
SC-003<\/a><\/td>\nCytoplasmic & Nuclear Extraction Kits<\/a><\/td>\n50 Preps<\/td>\n<\/tr>\n
SM-005<\/a><\/td>\nPlasma Membrane Protein Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
NE-013<\/a><\/td>\nNuclear Envelope Protein Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
MP-007<\/a><\/td>\nMitochondria Isolation Kit For Mammalian Cells And Tissues<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
AF-023<\/a><\/td>\nAdipose Tissue Fractionation Kit<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n
NI-024<\/a><\/td>\nDetergent-free nuclei isolation kit<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n
YM-017<\/a><\/td>\nYeast Mitochondria Enrichment Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
MM-018<\/a><\/td>\nPlant Microsomal Membrane Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
CP-011<\/a><\/td>\nChloroplast Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n
Specialty Kits<\/strong><\/td>\n<\/tr>\n
Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
PN-019<\/a><\/td>\nProtein\/Nucleic Acid Extraction Kit from Gels<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
SC-012<\/a><\/td>\nSingle Cell Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
HP-014<\/a><\/td>\nHistone\/DNA Binding Protein Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n
Protein Analysis Reagents<\/strong><\/td>\n<\/tr>\n
Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
WA-001<\/a><\/td>\nHRP Substrate Kit<\/a><\/td>\n30 ml\/30 ml<\/td>\n<\/tr>\n
WA-002<\/a><\/td>\nAntibody Enhancer<\/a><\/td>\n30 ml<\/td>\n<\/tr>\n
WA-003<\/a><\/td>\nWestern Blot Stripping Solution<\/a><\/td>\n250 ml<\/td>\n<\/tr>\n
WA-004<\/a><\/td>\nPonceau S Stain<\/a><\/td>\n250 ml<\/td>\n<\/tr>\n
WA-005<\/a><\/td>\nPeroxidase Suppressor<\/a><\/td>\n30 ml<\/td>\n<\/tr>\n
WA-006<\/a><\/td>\nHigh Efficiency Protein Precipitation kit<\/a><\/td>\n30 ml\/30 ml<\/td>\n<\/tr>\n
WA-007<\/a><\/td>\nRBC Lysis Buffer<\/a><\/td>\n250 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7522\u54c1\u540d\u7a31: Minute\u2122 \u00a0\u6838\u8cea\u86cb\u767d\u8403\u53d6\u5957\u7d44 | Cytoplasmic & Nuclear Ext […]<\/p>\n","protected":false},"author":3,"featured_media":7728,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[4193],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7727"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7727"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7727\/revisions"}],"predecessor-version":[{"id":9980,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7727\/revisions\/9980"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7728"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7727"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7727"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7727"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}