{"id":7718,"date":"2016-11-15T15:09:44","date_gmt":"2016-11-15T07:09:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7718"},"modified":"2024-05-17T15:04:54","modified_gmt":"2024-05-17T07:04:54","slug":"biotin%e6%8a%97%e9%ab%94%e8%9b%8b%e7%99%bd%e8%b3%aa%e6%a8%99%e8%aa%8c%e5%a5%97%e7%b5%84-type-a-innovabiosciences%e8%b2%a8%e8%99%9f370-0010","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7718","title":{"rendered":"Biotin\u6297\u9ad4\u86cb\u767d\u8cea\u6a19\u8a8c\u5957\u7d44 | (Type A) INNOVABIOSCIENCES\u8ca8\u865f#370-0010"},"content":{"rendered":"

Lightning-Link\u00ae Biotin (Type A)<\/strong>\u00a0| <\/strong>\u6297\u9ad4<\/strong>\u86cb\u767d\u8cea<\/strong>\u6a19\u8a8c\u5957\u7d44<\/strong><\/span> \u8ca8\u865f<\/strong>#370-0010<\/strong>\u5305\u88dd<\/strong>3x100UG<\/strong><\/span><\/p>\n

\u53ea\u9700\u4e00\u500b\u6b65\u9a5f\u4e0d\u9808Dialysis\u6216desalt\u6b65\u9a5f\u3002\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u8fd1\u4e4e100%\u3002<\/span><\/p>\n

 <\/p>\n

\u985e\u5225:Biotin \u6297\u9ad4\/\u86cb\u767d\u8cea\/\u80dc\u80bd\uff08amino group\uff09\u6a19\u8a8c<\/strong><\/p>\n

\u7522\u54c1\u654d\u8ff0:<\/strong> Biotin <\/strong>\u9175\u7d20\u6297\u9ad4\u6a19\u8a8cdirectly labeling kit (\u5feb\u901f\u578b\u8ca8\u865f#370-0010)\uff0c\u9996\u9078\u82f1\u570binnova biosciences\u516c\u53f8\u5c08\u5229\u7814\u767c\u4e4blightning-link\u6297\u9ad4\u87a2\u5149\u6a19\u8a8c\u5957\u7d44\u3002\u5e02\u9762\u4e0aantibody labeling kit\uff0c\u5728pH8~9\u74b0\u5883\u4e0b\u505a\u53cd\u61c9\u3002\u5982\u6b64\u5730\u74b0\u5883\u6613\u9020\u6210\u6297\u9ad4\u6d3b\u6027\u5931\u6548\uff0c\u5373\u4f7f\u7167\u8457\u539f\u5ee0\u63d0\u4f9b\u7684Protocol\u4e0b\u9032\u884c\u53cd\u61c9\uff0c\u6297\u9ad4\u56de\u6536\u7684\u6548\u80fd\u4e5f\u5f88\u5dee; \u592a\u591a\u6297\u9ad4\u672a\u88ab\u6a19\u8a8c\uff0c\u6240\u4ee5\u5fc5\u9808\u7d93\u7531Dialysis\u53cadesalt\u6b65\u9a5f\u79fb\u53bb\u672a\u7d50\u5408\u7684\u6297\u9ad4\u3002\u5982\u6b64\u4e00\u4f86\u6d6a\u8cbb\u4e86\u73cd\u8cb4\u7684\u6297\u9ad4, \u6297\u9ad4\u7d50\u5408\u6548\u80fd\u4e5f\u4e0d\u7406\u60f3\u3002Lightning-link\u5546\u54c1\u514b\u670d\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u4e0d\u7406\u60f3\u7684\u554f\u984c\uff0c\u5c08\u5229\u7814\u767c\u6539\u826f\uff0c\u6297\u9ad4\u6a19\u8a8c\u6548\u80fd\u8fd1\u4e4e100%\uff0c\u662f\u6a19\u8a8c\u7684\u9996\u9078\u5546\u54c1\u3002\u6a19\u8a8c\u6297\u9ad4\u7bc4\u570d10ug-1mg\uff0c\u5927\u91cf\u6a19\u8a8c\u8acb\u4f86\u96fb\u6d3d\u8a6202-86609496<\/a><\/p>\n

\u751f\u7269\u6a19\u8a8c\u7684\u5c08\u5bb6<\/strong>innova<\/strong> biosciences<\/strong><\/p>\n

\u82f1\u570bINNOVA BIOSCIENCES\uff0c\u4ee5\u751f\u7269\u7d50\u5408\u70ba\u4e3b\u8981\u4e8b\u696d\u3002\u65bc1996\u5e74\u5c08\u5229\u7814\u767c\uff0c\u4e2d\u6027\u74b0\u5883\u4e0b\u9032\u884c\u6297\u9ad4\u7d50\u5408\u53cd\u61c9\uff0c\u53ea\u8981\u4e00\u500b\u6b65\u9a5f\uff0c30\u79d2hands on time\u3002\u5176\u6297\u9ad4\u56de\u6536\u6548\u80fd\u8fd1\u4f3c100%\u3002\u4e0d\u9808\u518d\u505aDialysis\u6216desalt\u7684\u6b65\u9a5f\u3002Lightning-Link \u53ea\u8981\u4e00\u500b\u6b65\u9a5f\uff0c\u5373\u53ef\u5c07\u86cb\u767d\u8cea\/\u6297\u9ad4\/\u80dc\u80bd\u505a\u6a19\u8a8c\uff0c\u5177\u5c08\u5229\u7814\u767cQuencher reagent\u5316\u5b78\u53cd\u61c9\u5fb9\u5e95\u4e2d\u6b62\u6b98\u9918\u53cd\u61c9\u53ca\u505c\u6b62\u975e\u5c08\u4e00\u6027\u7d50\u5408\uff0c\u56e0\u6b64\u7121\u9808desalt\u53cadialysis\uff0c\u6297\u9ad4\u6a19\u8a8c\u56de\u6536\u7387100%\u3002<\/p>\n

Lightning-link<\/strong>\u6297\u9ad4\u6a19\u8a8c\u539f\u7406<\/strong><\/p>\n

Lightning-Link\u4ee5\u5171\u50f9\u9375\u7d50\uff0c\u8fa8\u8996amino groups\uff0c\u6027\u8cea\u6975\u70ba\u7a69\u5b9a\uff0c\u4e00\u6b21\u53cd\u61c9\u5f8c\uff0c\u53ef\u51b7\u51cd\u4fdd\u5b58\uff0c\u7bc0\u7701\u5be6\u9a57\u6642\u9593\u3002\"\"<\/p>\n

The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin<\/a><\/strong> proteins, including streptavidin<\/strong><\/a> and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA.\u00a0Our biotinylation kits are optimised to create high quality conjugates and minimise assay background. \u00a0Lightning-Link\u00ae Biotin has been optimised for two separate applications:\u00a0<\/strong>Type A –\u00a0<\/strong>intended for assays in which a streptavidin-labeled detection reagent will be used,\u00a0Type B –\u00a0<\/strong>optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).<\/p>\n

Lightning-link<\/strong>\u6297\u9ad4\u6a19\u8a8c\u61c9\u7528<\/strong><\/p>\n

\u53ef\u4ee5\u61c9\u7528\u5728ELISA. immunofluorescent. Flow. confocol. Western Blot\u7b49\u6aa2\u8a3a\u53ca\u5206\u5b50\u75c5\u6bd2\u4e4b\u7814\u7a76!!\u76f4\u63a5\u5728\u4e00\u6297\u4e0a\u505a\u6a19\u8a8c (\u53ef\u6a19\u8a8c\u87a2\u5149\u86cb\u767d \u87a2\u5149dye HRP Biotin\u7b49) \uff0c\u514b\u670d\u4e8c\u6297\u6240\u9020\u6210\u7684\u80cc\u666f\u503c\u904e\u9ad8\u53caHost\u4f86\u6e90\u4e0d\u540c\u6240\u9020\u6210Crosstalk\u7684\u73fe\u8c61\u3002Lightning-link\u6297\u9ad4\u6a19\u8a8cV.S.\u4fdd\u5b58\u6db2\u00a0<\/strong>\u6297\u9ad4\u4fdd\u5b58\u65bcAzide\u3001Gelatin\u53caBSA\u6eb6\u6db2\u4e0b\uff0c\u6703\u5e72\u64fe\u4e00\u822c\u7d50\u5408\u7684\u6548\u679c\uff0c\u6240\u4ee5\u6703\u518d\u7d93\u904e\u7d14\u5316\u6b65\u9a5f\uff0c\u6d6a\u8cbb\u8a31\u591a\u6297\u9ad4\u3002Lightning-Link\u514b\u670dAzide\u3001Gelatin\u53caBSA\u5e72\u64fe\u7d50\u5408\u7684\u6548\u7387\uff0c\u7701\u4e0b\u60a8\u5bf6\u8cb4\u7684\u6297\u9ad4\u3002\u5225\u65bc\u5176\u5b83\u7d50\u5408\u7684\u65b9\u5f0f\uff0cLightning-Link\u7121\u9808\u5728High pH\u7684\u6eb6\u6db2\u4e0b\u64cd\u4f5c\uff0c\u6240\u4ee5\u53ea\u8981\u662f\u5177\u6709amino groups (lysin\/a-amino)\u7684\u86cb\u767d\u8cea\/\u80dc\u80bd\/\u6297\u9ad4\uff0c\u90fd\u53ef\u53cd\u61c9\u3002<\/p>\n

\u66f4\u591a\u6297\u9ad4\u6a19\u8a8c\u76f8\u95dc<\/span><\/strong>\u00a0 InnovaGold\u5948\u7c73\u91d1\u6a19\u8a8c\u5feb\u7be9\u8a66\u5291<\/a> | Lightning-link\u9175\u7d20\u578b\u6297\u9ad4\u6a19\u8a8c<\/a> | Abselect\u6297\u9ad4\u7d14\u5316 <\/a>| SBA\u55ae\u682a\u6297\u9ad4\u5206\u578bELISA<\/a> | Lightning-link\u87a2\u5149\u86cb\u767d\u6297\u9ad4\u6a19\u8a8c<\/a> | Thunder-link\u6838\u9178\u6297\u9ad4\u6a19\u8a8c<\/a><\/span><\/strong><\/p>\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
370-0010<\/td>\nLightning-Link\u00ae\u00a0Biotin<\/td>\n3×100\u03bcg<\/td>\n<\/tr>\n
717-0010<\/td>\nLightning-Link\u00ae\u00a0Avidin<\/td>\n3×100\u03bcg<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6297\u9ad4\u6a19\u8a18\u6cd5\u6ce8\u610f\u4e8b\u9805-\u7de9\u885d\u6db2\u548c\u6dfb\u52a0\u5291\u3001\u6297\u9ad4\u6fc3\u5ea6\u548c\u7d14\u5ea6<\/span><\/strong><\/span><\/p>\n

<\/p>\n

\u6297\u9ad4\u7531\u80fa\u57fa\u9178\u7d44\u6210\uff1b\u8cf4\u6c28\u9178\u5074\u93c8\uff08\u5e36\u6709 -NH2 \u672b\u7aef\uff0c\u4f2f\u80fa\uff09\u901a\u5e38\u7528\u65bc\u5c07\u6a19\u8a18\u7269\u5171\u50f9\u9023\u63a5\u5230\u6297\u9ad4\u5206\u5b50\u3002\u53ea\u6709\u56db\u7a2e\u5e38\u898b\u7684\u6a19\u8a18\u65b9\u6cd5\uff0c\u5176\u4e0d\u540c\u4e4b\u8655\u5728\u65bc\u53cd\u61c9\u6027\u6a19\u8a18\u7684\u5275\u5efa\u3002<\/p>\n

\u6297\u9ad4\u6a19\u8a18\u7684\u56db\u7a2e\u4e3b\u8981\u5316\u5b78\u65b9\u6cd5\u7e3d\u7d50\u5982\u4e0b\uff1a<\/p>\n

    \n
  1. NHS \u916f\uff1a<\/strong>\u5c0d\u65bc\u87a2\u5149\u67d3\u6599\u6a19\u7c64\uff0c\u901a\u5e38\u6703\u8cfc\u8cb7\u5e36\u6709\u5167\u5efa NHS \u916f\uff08\u4e5f\u7a31\u70ba\u300c\u7425\u73c0\u91af\u4e9e\u80fa\u916f\u300d\uff09\u7684\u6d3b\u5316\u5f62\u5f0f\u7684\u6a19\u7c64\u3002\u6d3b\u5316\u7684\u67d3\u6599\u53ef\u4ee5\u5728\u9069\u7576\u7684\u689d\u4ef6\u4e0b\u8207\u6297\u9ad4\uff08\u6240\u6709\u6297\u9ad4\u90fd\u5177\u6709\u591a\u500b\u8cf4\u6c28\u9178\u57fa\u5718\uff09\u53cd\u61c9\u3002\u5728\u5c07\u6a19\u8a18\u7684\u6297\u9ad4\u7528\u65bc\u514d\u75ab\u6e2c\u5b9a\u4e4b\u524d\uff0c\u900f\u904e\u5e7e\u7a2e\u53ef\u80fd\u7684\u65b9\u6cd5\uff08\u901a\u5e38\u662f\u67f1\u5c64\u6790\u6cd5\uff09\u4e4b\u4e00\u53bb\u9664\u904e\u91cf\u7684\u6d3b\u6027\u67d3\u6599\u3002<\/li>\n
  2. \u7570\u96d9\u529f\u80fd\u8a66\u5291\uff1a<\/strong>\u5982\u679c\u6a19\u8a18\u662f\u86cb\u767d\u8cea\u5206\u5b50\uff08\u4f8b\u5982HRP\u3001\u9e7c\u6027\u78f7\u9178\u9176\u6216\u85fb\u7d05\u86cb\u767d\uff09\uff0c\u5247\u6297\u9ad4\u6a19\u8a18\u904e\u7a0b\u6703\u56e0\u70ba\u6297\u9ad4\u548c\u6a19\u8a18\u5177\u6709\u591a\u500b\u80fa\u800c\u8b8a\u5f97\u8907\u96dc\u3002\u5728\u9019\u7a2e\u60c5\u6cc1\u4e0b\uff0c\u901a\u5e38\u4fee\u98fe\u4e00\u500b\u5206\u5b50\uff08\u4f8b\u5982\u6297\u9ad4\uff09\u4e0a\u7684\u4e00\u4e9b\u8cf4\u6c28\u9178\u4ee5\u5275\u5efa\u65b0\u7684\u53cd\u61c9\u57fa\u5718\uff08X\uff09\uff0c\u4e26\u4fee\u98fe\u6a19\u7c64\u4e0a\u7684\u8cf4\u6c28\u9178\u4ee5\u5275\u5efa\u53e6\u4e00\u500b\u53cd\u61c9\u57fa\u5718\uff08Y\uff09\u3002 \u300c\u7570\u96d9\u529f\u80fd\u8a66\u5291\u300d\u7528\u65bc\u5f15\u5165 Y \u57fa\u5718\uff0c\u96a8\u5f8c\u7576\u6297\u9ad4\u548c\u6a19\u8a18\u6df7\u5408\u6642\uff0cY \u57fa\u5718\u8207 X \u57fa\u5718\u767c\u751f\u53cd\u61c9\uff0c\u5f9e\u800c\u7522\u751f\u7570\u4e8c\u805a\u9ad4\u7db4\u5408\u7269\u3002\u9019\u500b\u4e3b\u984c\u6709\u5f88\u591a\u8b8a\u5316\uff0c\u60a8\u6703\u5728\u6587\u737b\u4e2d\u627e\u5230\u6578\u767e\u500b\u95dc\u65bc\u4f7f\u7528\u7570\u96d9\u529f\u80fd\u8a66\u5291\u4f86\u5275\u5efa\u6a19\u8a18\u6297\u9ad4\u548c\u5176\u4ed6\u6a19\u8a18\u751f\u7269\u5206\u5b50\u7684\u4f8b\u5b50\u3002<\/li>\n
  3. \u78b3\u4e8c\u4e9e\u80fa\uff1a<\/strong>\u9019\u4e9b\u8a66\u5291\uff08EDC \u662f\u4e00\u500b\u975e\u5e38\u5e38\u898b\u7684\u4f8b\u5b50\uff09\u7528\u65bc\u5728\u542b\u80fa\u5206\u5b50\u548c\u542b\u7fa7\u57fa\u5206\u5b50\u4e4b\u9593\u5efa\u7acb\u5171\u50f9\u9023\u63a5\u3002\u78b3\u4e8c\u4e9e\u80fa\u6703\u6d3b\u5316\u7fa7\u57fa\uff0c\u7136\u5f8c\u6d3b\u5316\u7684\u4e2d\u9593\u9ad4\u88ab\u80fa\uff08\u4f8b\u5982\u7531\u6297\u9ad4\u4e0a\u7684\u8cf4\u6c28\u9178\u6b98\u57fa\u63d0\u4f9b\uff09\u653b\u64ca\u3002\u78b3\u4e8c\u4e9e\u80fa\u901a\u5e38\u7528\u65bc\u5c07\u6297\u9ad4\u8207\u7fa7\u5316\u9846\u7c92\uff08\u4f8b\u5982\u4e73\u81a0\u9846\u7c92\u3001\u78c1\u73e0\uff09\u4ee5\u53ca\u5176\u4ed6\u7fa7\u5316\u8868\u9762\uff08\u4f8b\u5982\u5fae\u5b54\u677f\u6216\u6676\u7247\u8868\u9762\uff09\u7db4\u5408\u3002\u78b3\u4e8c\u4e9e\u80fa\u5f88\u5c11\u7528\u65bc\u5c07\u67d3\u6599\u6216\u86cb\u767d\u8cea\u6a19\u8a18\u9644\u8457\u5230\u6297\u9ad4\u4e0a\uff0c\u5118\u7ba1\u5b83\u5011\u5728 NHS \u6d3b\u5316\u67d3\u6599\u7684\u751f\u7522\u4e2d\u5f88\u91cd\u8981\uff08\u898b\u4e0a\u6587\uff09\u3002<\/li>\n
  4. \u9ad8\u7898\u9178\u9209\uff1a<\/strong>\u9019\u7a2e\u5316\u5b78\u7269\u8cea\u4e0d\u80fd\u8207\u7d55\u5927\u591a\u6578\u6a19\u8a18\u4e00\u8d77\u4f7f\u7528\uff0c\u4f46\u5b83\u662f\u4e00\u7a2e\u975e\u5e38\u91cd\u8981\u7684\u8a66\u5291\uff0c\u56e0\u70ba\u5b83\u9069\u7528\u65bc\u6700\u53d7\u6b61\u8fce\u7684\u8a3a\u65b7\u9176 HRP\u3002\u9ad8\u7898\u9178\u9e7d\u6703\u6d3b\u5316 HRP \u5206\u5b50\u4e0a\u7684\u78b3\u6c34\u5316\u5408\u7269\u93c8\u4ee5\u7522\u751f\u919b\u57fa\uff0c\u8a72\u919b\u57fa\u80fd\u5920\u8207\u6297\u9ad4\u5206\u5b50\u4e0a\u7684\u96e2\u80fa\u9178\u53cd\u61c9\u3002\u7531\u65bc HRP \u672c\u8eab\u542b\u6709\u5f88\u5c11\u7684\u96e2\u80fa\u9178\uff0c\u56e0\u6b64\u76f8\u5c0d\u5bb9\u6613\u7522\u751f\u6297\u9ad4-HRP \u7db4\u5408\u7269\uff0c\u800c\u7121\u9700\u986f\u8457\u7684 HRP \u805a\u5408\u3002<\/li>\n<\/ol>\n

    \u60a8\u53ef\u4ee5\u5f9e\u4efb\u4f55\u6709\u95dc\u6297\u9ad4\u6a19\u8a18\u7684\u5c08\u696d\u66f8\u7c4d\u4e2d\u627e\u5230\u6709\u95dc\u9019\u56db\u7a2e\u65b9\u6cd5\u7684\u66f4\u8a73\u7d30\u8aaa\u660e\u3002<\/p>\n

    \u7de9\u885d\u6db2\u548c\u6dfb\u52a0\u5291 – \u6297\u9ad4\u6a19\u8a18\u7684\u95dc\u9375\u6ce8\u610f\u4e8b\u9805(Buffers and Additives – Key Considerations for Antibody labeling)<\/strong><\/span><\/p>\n

    \u91cd\u8981\u7684\u662f\u8981\u8a18\u4f4f\uff0c\u60a8\u7684\u6297\u9ad4\u5c07\u542b\u6709\u6297\u9ad4\u4ee5\u5916\u7684\u7269\u8cea\uff1b\u81f3\u5c11\u7de9\u885d\u6db2\u548c\/\u6216\u9e7d\uff0c\u4ee5\u53ca\u53ef\u80fd\u7684\u5176\u4ed6\u86cb\u767d\u8cea\u548c\u6dfb\u52a0\u5291\u3002\u7576\u6297\u9ad4\u6700\u521d\u88ab\u7d14\u5316\u548c\u914d\u88fd\u6642\uff0c\u6df7\u5408\u7269\u8207\u6a19\u8a18\u65b9\u6cd5\u7684\u517c\u5bb9\u6027\u53ef\u80fd\u4e0d\u662f\u95dc\u9375\u8003\u616e\u56e0\u7d20\uff1b\u6709\u6642\u5728\u9032\u884c\u6a19\u8a18\u53cd\u61c9\u4e4b\u524d\u9700\u8981\u91cd\u65b0\u7d14\u5316\u6297\u9ad4\u3002\u7d14\u5316(Purification)\u53ef\u80fd\u6d89\u53ca\u53bb\u9664\u7a69\u5b9a\u86cb\u767d(stabilizing proteins)\uff08\u4f8b\u5982 BSA\uff09\u6216\u53bb\u9664\u4f4e\u5206\u5b50\u91cf\u7269\u8cea(low molecular weight substances)\uff0c\u4f8b\u5982\u758a\u6c2e\u5316\u9209(sodium azide)\u3001tris \u7de9\u885d\u6db2(tris buffer )\u6216\u7518\u6c28\u9178(glycine)\u3002\u4e0d\u540c\u7684\u6a19\u8a18\u65b9\u6cd5\u53ef\u80fd\u6703\u53d7\u5230\u4e0d\u540c\u7a0b\u5ea6\u7684\u4e0d\u540c\u6dfb\u52a0\u5291\u7684\u8ca0\u9762\u5f71\u97ff\uff0c\u4f46\u5982\u524d\u6240\u8ff0\uff0c\u5927\u591a\u6578\u6a19\u8a18\u65b9\u6cd5\u5229\u7528\u6297\u9ad4\u4e0a\u7684\u8cf4\u6c28\u9178\u6b98\u57fa(lysine residues )\uff0c\u56e0\u6b64\u5728\u6297\u9ad4\u6a19\u8a18\u904e\u7a0b\u4e2d\u901a\u5e38\u61c9\u907f\u514d\u4f7f\u7528\u5177\u6709\u4f2f\u80fa(primary amines )\u7684\u7269\u8cea\u3002\u4f8b\u5982\uff0c\u7518\u6c28\u9178(glycine)\u901a\u5e38\u7528\u65bc\u5f9e\u6297\u539f\u89aa\u548c\u67f1\u4e0a\u6d17\u812b\u6297\u9ad4(elute antibodies)\uff0c\u4e26\u4e14\u4ee5\u9019\u7a2e\u65b9\u5f0f\u7d14\u5316\u7684\u6297\u9ad4\u61c9\u5728\u7528\u65bc\u6a19\u8a18\u53cd\u61c9\u4e4b\u524d\u9032\u884c\u900f\u6790(dialysed)\u3002\u4eba\u5011\u61c9\u8a72\u7279\u5225\u6ce8\u610f\u900f\u6790(dialysis )\uff0c\u5982\u679c\u66f4\u63db\u7de9\u885d\u6db2\u5169\u5230\u4e09\u6b21\uff0c\u53bb\u9664\u4e0d\u9700\u8981\u7684\u4f4e\u5206\u5b50\u91cf\u7269\u8cea\u6703\u66f4\u6709\u6548\u3002\u9019\u4e26\u4e0d\u610f\u5473\u8457\u60a8\u5fc5\u9808\u88fd\u4f5c\u4e09\u500d\u7684\u7de9\u885d\u6db2\uff0c\u6070\u6070\u76f8\u53cd\uff0c\u5118\u7ba1\u900f\u6790\u904e\u7a0b\u6240\u9700\u7684\u7e3d\u6642\u9593\u6703\u66f4\u9577\u3002\u4f8b\u5982\uff0c\u8a08\u7b97\u8868\u660e\uff0c\u91dd\u5c0d 3 x 1L \u9ad4\u7a4d\u7684\u900f\u6790\u6bd4\u91dd\u5c0d\u55ae\u4e00 5L \u9ad4\u7a4d\u7684\u900f\u6790\u66f4\u6709\u6548\u3002\u901a\u5e38\u5efa\u8b70\u4f7f\u7528 PBS\u3001MES \u6216\u78b3\u9178\u6c2b\u9e7d\u4f5c\u70ba\u9032\u884c\u7db4\u5408\u53cd\u61c9\u7684\u5408\u9069\u7de9\u885d\u6db2\uff1b\u6700\u4f73\u7de9\u885d\u6db2\u6839\u64da\u6a19\u8a18\u53cd\u61c9\u7684 pH \u8981\u6c42\u4f86\u6c7a\u5b9a\u3002<\/p>\n

    \u6297\u9ad4\u6fc3\u5ea6\u548c\u7d14\u5ea6 (Antibody Concentration and Purity)\u00a0<\/strong><\/span><\/p>\n

    \u5c0d\u65bc\u5927\u591a\u6578\u6a19\u8a18\u53cd\u61c9\uff0c\u6297\u9ad4\u9700\u8981\u76f8\u7576\u7d14\u6de8\uff08\u4f8b\u5982>90%\uff0c\u512a\u9078>95%\uff09\u4e26\u4e14\u6fc3\u5ea6>0.5mg\/ml\u3002\u8a31\u591a\u5e02\u552e\u6297\u9ad4\u90fd\u4ee5\u9069\u5408\u6a19\u8a18\u7684\u5f62\u5f0f\u63d0\u4f9b\uff0c\u4f46\u60a8\u61c9\u8a72\u610f\u8b58\u5230\u60c5\u6cc1\u4e26\u975e\u7e3d\u662f\u5982\u6b64\u3002\u4f8b\u5982\uff0c\u6297\u9ad4\u53ef\u4ee5\u4ee5\u96dc\u4ea4\u7624\u7d44\u7e54\u57f9\u990a\u4e0a\u6e05\u6db2(TCS)\u3001\u8179\u6c34\u6216\u7c97\u8840\u6e05\u7684\u5f62\u5f0f\u51fa\u552e\u3002 TCS \u901a\u5e38\u542b\u6709\u8a31\u591a\u5176\u4ed6\u86cb\u767d\u8cea\u548c\u57f9\u990a\u7269\u71df\u990a\u7269\u8cea\uff08\u4f8b\u5982\u80fa\u57fa\u9178\uff09\uff0c\u9019\u662f\u7279\u5225\u6709\u554f\u984c\u7684\u3002\u5982\u679c TCS \u662f\u60a8\u7684\u8d77\u9ede\uff0c\u5247\u6216\u591a\u6216\u5c11\u5fc5\u9808\u9032\u884c\u7d14\u5316\uff08\u4f8b\u5982\u5728\u86cb\u767d A \u67f1\u4e0a\uff09\u3002\u8179\u6c34\u548c\u7c97\u8840\u6e05\u7684\u6297\u9ad4\u6fc3\u5ea6\u6bd4TCS\u9ad8\uff0c\u4f46\u9019\u4e9b\u6750\u6599\u4e0d\u7d14\u4e14\u542b\u6709\u9ad8\u6fc3\u5ea6\u7684\u80fa\u57fa\u9178\uff1b\u901a\u5e38\u9700\u8981\u9032\u4e00\u6b65\u7d14\u5316\u6297\u9ad4\u3002<\/p>\n

    \u6b64\u6642\uff0c\u63d0\u4f9b\u4e0a\u8ff0\u5167\u5bb9\u7684\u7e3d\u7d50\u53ef\u80fd\u5f88\u6709\u7528\uff1a\u60a8\u73fe\u5728\u4e86\u89e3 (i) \u76f4\u63a5\u6aa2\u6e2c\u548c\u9593\u63a5\u6aa2\u6e2c\u4e4b\u9593\u7684\u4e3b\u8981\u5dee\u7570\uff1b (ii) \u6297\u9ad4\u6a19\u8a18\u7684\u56db\u7a2e\u4e3b\u8981\u65b9\u6cd5\uff1b (iii) \u7de9\u885d\u6db2\u914d\u65b9\u7684\u91cd\u8981\u6027\uff1b (iv) \u6297\u9ad4\u6fc3\u5ea6\u3002<\/p>\n

    \u4e8b\u5be6\u4e0a\uff0c\u5982\u4eca\uff0c\u7531\u65bc\u6297\u9ad4\u6a19\u8a18\u6280\u8853\u7684\u9032\u6b65\uff0c\u4efb\u4f55\u6703\u4f7f\u7528\u624b\u6301\u5f0f\u79fb\u6db2\u7ba1\u7684\u4eba\u90fd\u53ef\u4ee5\u8f15\u9b06\u88fd\u4f5c\u6a19\u8a18\u6297\u9ad4\u3002\u4ee5\u4e0b\u8a0e\u8ad6 Lightning-Link \u4e00\u6b65\u5f0f\u6297\u9ad4\u6a19\u8a18\u65b9\u6cd5<\/p>\n

    Lightning-Link – \u4e16\u754c\u4e0a\u6700\u7c21\u55ae\u7684\u6297\u9ad4\u6a19\u8a18\u8a66\u5291\u76d2<\/strong><\/span><\/p>\n

    \u6297\u9ad4\u6a19\u8a18\u904e\u7a0b\u7684\u7c21\u5316\uff08\u6700\u986f\u8457\u7684\u662f\u6d88\u9664\u67f1\u5206\u96e2\u6b65\u9a5f\uff09\u907f\u514d\u4e86\u591a\u5e74\u4f86\u56f0\u64fe\u50b3\u7d71\u7a0b\u5e8f\u7684\u8a31\u591a\u554f\u984c\u2014\u2014\u6750\u6599\u640d\u5931\u3001\u67f1\u5c64\u6790\u904e\u7a0b\u4e2d\u7684\u6a23\u54c1\u7a00\u91cb\u3001\u6279\u6b21\u9593\u5dee\u7570\u4ee5\u53ca\u64f4\u5927\u898f\u6a21\u7684\u56f0\u96e3\u3002<\/p>\n

    Lightning-Link \u6d41\u7a0b\u5982\u5716 1 \u6240\u793a\u3002\u5c0f\u74f6\u5167\u5bb9\u7269\u7684\u6eb6\u89e3\u6703\u6d3b\u5316\u4ecb\u5c0e\u6297\u9ad4\u6a19\u8a18\u53cd\u61c9\u7684\u5316\u5b78\u7269\u8cea\u3002\u7531\u65bc\u6c92\u6709\u7d14\u5316\u6216\u5206\u96e2\u6b65\u9a5f\uff08\u53cd\u61c9\u526f\u7522\u7269\u5b8c\u5168\u662f\u826f\u6027\u7684\uff09\uff0c\u6297\u9ad4\u56de\u6536\u7387\u63a5\u8fd1 100%\u3002\u6b64\u5916\uff0c\u4f7f\u7528\u9019\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\u53ef\u4ee5\u5728\u4e0d\u5230\u4e09\u5341\u79d2\u7684\u6642\u9593\u5167\u5efa\u7acb\u6a19\u8a18\u53cd\u61c9\u3002<\/p>\n

    \u5716 1\uff1aLightning-Link \u6297\u9ad4\u6a19\u8a18\u6d41\u7a0b<\/strong><\/span><\/p>\n

    \"Lightning-Link<\/strong><\/p>\n

    \u5118\u7ba1\u6297\u9ad4\u6a19\u8a18\u7a0b\u5e8f\u975e\u5e38\u7c21\u55ae\uff0c\u4f46\u5316\u5b78\u65b9\u6cd5\u537b\u5f88\u8907\u96dc\u2014\u2014\u50c5\u5141\u8a31\u5728\u6eab\u548c\u4e14\u53d7\u63a7\u7684\u904e\u7a0b\u4e2d\u5f62\u6210\u6297\u9ad4\u6a19\u8a18\u7db4\u5408\u7269\u3002\u7528 Lightning-Link \u6a19\u8a18\u7684\u6297\u9ad4\u8868\u73fe\u51fa\u7684\u6027\u80fd\u7279\u5fb5\u8207\u900f\u904e\u8cbb\u529b\u7684\u591a\u6b65\u9a5f\u50b3\u7d71\u7a0b\u5e8f\u88fd\u5099\u7684\u6297\u9ad4\u76f8\u540c\u6216\u66f4\u597d – \u8acb\u53c3\u898b\u4e0b\u5716 2\u3002<\/p>\n

    \u5716 2\uff1a\u4f7f\u7528\u6a19\u6e96\u65b9\u6cd5\u8207 Lightning-Link \u88fd\u5099\u7684\u7db4\u5408\u7269\u7684 ELISA<\/strong><\/span><\/p>\n

    \"\u4f7f\u7528\u6a19\u6e96\u54c1\u8207<\/strong><\/p>\n

    Lightning-Link \u65b9\u6cd5\u7684\u7c21\u55ae\u6027\u610f\u5473\u8457\u53ef\u4ee5\u8f15\u9b06\u653e\u5927\uff08\u6216\u7e2e\u5c0f\uff09\u5f85\u6a19\u8a18\u7684\u6297\u9ad4\u91cf\u3002\u7121\u8ad6\u60a8\u662f\u6a19\u8a18\u5c11\u91cf\u6297\u9ad4\u9084\u662f\u5927\u91cf\u6297\u9ad4\uff0c\u64cd\u4f5c\u6642\u9593\u90fd\u4fdd\u6301\u4e0d\u8b8a \u2013 \u53ea\u9700 30 \u79d2\u3002\u7531\u65bc\u64f4\u5927\u898f\u6a21\u4e0d\u6703\u7522\u751f\u65b0\u7684\u6280\u8853\u554f\u984c\uff0c\u56e0\u6b64\u5c0f\u898f\u6a21\u88fd\u5099\u7684\u8a66\u9a57\u7db4\u5408\u7269\u7684\u6027\u80fd\u8207\u5927\u898f\u6a21\u88fd\u5099\u7684\u8a66\u9a57\u7db4\u5408\u7269\u7684\u6027\u80fd\u57fa\u672c\u76f8\u540c\uff08\u53c3\u898b\u4e0b\u5716 3\uff09\u3002\u5c0d\u65bc\u4efb\u4f55\u65b0\u6297\u9ad4\uff0c\u6216\u5728\u6297\u9ad4\u6975\u5176\u6709\u50f9\u503c\u4e14\u4f9b\u61c9\u6709\u9650\u7684\u60c5\u6cc1\u4e0b\uff0cLightning-Link \u8a66\u5291\u76d2\u5141\u8a31\u4f7f\u7528\u5c11\u81f3 10\u03bcg \u7684\u6297\u9ad4\u9032\u884c\u6a19\u8a18\u53cd\u61c9\u3002\u5176\u4ed6\u5e38\u898f\u5305\u88dd\u898f\u683c\u5305\u62ec 100ug\u30011mg \u548c 5mg\u3002<\/p>\n

    \u5716 3\uff1a\u4f7f\u7528 Lightning-Link \u9032\u884c\u4e0d\u540c\u5c3a\u5ea6\u6a19\u8a18\u7684\u6297\u9ad4\u7684 ELISA<\/strong><\/span><\/p>\n

    \"\u4f7f\u7528<\/strong><\/p>\n

    \u5118\u7ba1\u73fe\u5728\u9032\u884c\u6297\u9ad4\u6a19\u8a18\u53cd\u61c9\u7684\u5be6\u969b\u64cd\u4f5c\u975e\u5e38\u7c21\u55ae\uff1b\u5982\u4e0a\u6240\u8ff0\uff0c\u5728\u9032\u884c\u6a19\u8a18\u53cd\u61c9\u4e4b\u524d\uff0c\u4ecd\u6709\u5fc5\u8981\u8003\u616e\u6297\u9ad4\u88fd\u5099\u4e2d\u9084\u5b58\u5728\u4ec0\u9ebc\u3002\u7136\u800c\uff0cLightning-Link \u5c0d\u6297\u9ad4\u88fd\u5291\u4e2d\u7684\u8a31\u591a\u6dfb\u52a0\u5291\u5177\u6709\u986f\u8457\u7684\u8010\u53d7\u6027\u3002<\/p>\n

    \u758a\u6c2e\u5316\u9209\u5ee3\u6cdb\u7528\u4f5c\u5e02\u552e\u6297\u9ad4\u4e2d\u7684\u6297\u83cc\u5291\uff0c\u5176\u542b\u91cf\u901a\u5e38\u70ba 0.02% \u6216 0.1%\uff0c\u6703\u5c0d\u67d0\u4e9b\u6a19\u8a18\u65b9\u6cd5\u9020\u6210\u56b4\u91cd\u5e72\u64fe\u3002\u5e78\u904b\u7684\u662f\uff0cLightning-Link<\/strong>\u65b9\u6cd5\u53d7\u6fc3\u5ea6\u70ba 0.1% \u6216\u66f4\u4f4e\u7684\u758a\u6c2e\u5316\u9209\uff08\u5716 4\uff09\u7684\u5f71\u97ff\u6975\u5c0f\u3002<\/p>\n

    \u5716 4\uff1a\u5728\u6709\/\u6c92\u6709\u758a\u6c2e\u5316\u9209\u7684\u60c5\u6cc1\u4e0b\u4f7f\u7528\u6a19\u8a18\u7684\u6297\u9ad4\u9032\u884c ELISA<\/strong><\/span><\/p>\n

    \"\u5728\u6709\/\u6c92\u6709\u758a\u6c2e\u5316\u9209\u7684\u60c5\u6cc1\u4e0b\u4f7f\u7528\u6a19\u8a18\u7684\u6297\u9ad4\u9032\u884c<\/strong><\/p>\n

    \u5e02\u552e\u6297\u9ad4\u53e6\u4e00\u7a2e\u5e38\u898b\u7684\u6dfb\u52a0\u5291\u662f BSA\uff0c\u7528\u4f5c\u7a69\u5b9a\u5291\u3002\u96d6\u7136\u4eba\u5011\u9810\u671f BSA \u7b49\u5206\u5b50\u6703\u5c0d\u6a19\u8a18\u53cd\u61c9\u7522\u751f\u56b4\u91cd\u5e72\u64fe\uff0c\u4f46 BSA \u5c0d Lightning-Link \u7684\u5f71\u97ff\u975e\u5e38\u6709\u9650\u3002\u4e8b\u5be6\u4e0a\uff0c\u5f9e\u6975\u5c11\u91cf\u6297\u9ad4\u4e2d\u53bb\u9664 BSA \u7684\u53ef\u80fd\u597d\u8655\u53ef\u80fd\u6703\u88ab\u4efb\u4f55\u5617\u8a66\u7d14\u5316\u904e\u7a0b\u4e2d\u6297\u9ad4\u7684\u640d\u5931\u6240\u62b5\u6d88\u3002\u4f8b\u5982\uff0c\u5c0d\u65bc 1% BSA\uff0c\u76f8\u7576\u65bc\u6bd4 1mg\/ml \u6297\u9ad4\u6469\u723e\u904e\u91cf 20 \u500d\uff0c\u53ea\u9700\u5c07 BSA \u5b58\u5728\u4e0b\u6a19\u8a18\u7684\u6297\u9ad4\u7a00\u91cb 1\/3000\uff08\u800c\u4e0d\u662f 1\/\uff09\u5373\u53ef\u7372\u5f97\u6b63\u5e38 ELISA \u5438\u5149\u5ea6\u4e0d\u542bBSA \u7684\u53cd\u61c9\u70ba10,000\uff08\u898b\u57165\uff09\u3002<\/p>\n

    \u5716 5\uff1a\u5728 BSA \u5b58\u5728\u4e0b\u88fd\u5099\u7684\u6a19\u8a18\u6297\u9ad4\u7684 ELISA<\/span><\/strong><\/span><\/p>\n

    \"BSA<\/strong><\/p>\n

    \"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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