{"id":7535,"date":"2016-11-10T13:04:06","date_gmt":"2016-11-10T05:04:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7535"},"modified":"2024-12-10T11:07:56","modified_gmt":"2024-12-10T03:07:56","slug":"%e8%86%a0%e9%ab%94%e8%9b%8b%e7%99%bd%e8%b3%aa%e5%8f%8a%e6%a0%b8%e9%85%b8%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-invent%e8%b2%a8%e8%99%9fpn-019","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7535","title":{"rendered":"\u81a0\u9ad4\u86cb\u767d\u8cea\u53ca\u6838\u9178\u8403\u53d6\u5957\u7d44 |MALD-MS\u5206\u6790\u86cb\u767d\u6a23\u54c1\u524d\u8655\u7406\u7d14\u5316\u8a66\u5291 Invent\u8ca8\u865fPN-019"},"content":{"rendered":"

Minute\u2122<\/strong>\u81a0\u9ad4\u86cb\u767d\u8cea\u53ca\u6838\u9178<\/strong>\u8403\u53d6\u5957\u7d44<\/strong> | <\/strong>Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels| \u8ca8\u865f<\/strong>PN-019<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u88ab\u52d5elution(Passive elution)\u548c\u96fb\u6d17\u812b(electro elution)\u662f\u5f9e\u805a\u4e19\u70ef\u9170\u80fa\/\u74ca\u8102\u7cd6\u51dd\u81a0(polyacrylamide\/agarose gels)\u4e2d\u63d0\u53d6\u86cb\u767d\u8cea\u548c\/\u6216\u6838\u9178\u7684\u6700\u5e38\u898b\u7684\u65b9\u6cd5\u3002\u901a\u904e\u88ab\u52d5\u6d17\u812b\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u901a\u5e38\u9700\u8981overnight incubation \u3002elution\u86cb\u767d\u88ab\u986f\u8457\u7a00\u91cb\u4e26\u9700\u8981\u9032\u4e00\u6b65\u6fc3\u7e2e\u3002\u901a\u904e\u96fb\u6cf3elution\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u6bd4\u88ab\u52d5elution\u66f4\u5feb\uff0830\u5206\u9418\u81f32\u5c0f\u6642\uff09\uff0c\u4f46\u662f\u5176\u9700\u8981\u5c0d\u65bc\u8f03\u5927\u86cb\u767d\u8cea\uff08> 70Kda\uff09\u8f03\u4e0d\u6709\u6548\u5730\u8d77\u4f5c\u7528\u7684\u7279\u6b8aelution\u88dd\u7f6e\u3002\u96fb\u7de9\u885d\u6db2\u901a\u5e38\u542b\u6709\u53ef\u80fd\u5e72\u64fe\u4e0b\u6e38\u61c9\u7528\u7684\u6fc3\u5ea6\u7684\u6d17\u6ecc\u5291\u548c\u5176\u5b83\u5316\u5b78\u54c1\u3002Invent\u63d0\u4f9b\u4e86\u4e00\u500b\u5feb\u901f\uff0c\u7121\u5100\u5668\u7684\u86cb\u767d\u8cea\/\u6838\u9178\u63d0\u53d6\u8a66\u5291\u76d2\u3002\u86cb\u767d\u8cea\/\u6838\u9178\u53ef\u4ee5\u5728<10\u5206\u9418\u5167\u4ee5\u9ad8\u7522\u7387\u5f9e\u51dd\u81a0\u4e2d\u63d0\u53d6\u3002\u6d17\u812b\u7de9\u885d\u6db2 (elution buffers) \u53ef\u4ee5\u662f\u542b\u6d17\u6ecc\u5291\u7684\u7de9\u885d\u6db2\u6216\u7d14\u6c34\uff0c\u53d6\u6c7a\u65bc\u51dd\u81a0\u67d3\u8272\u7684\u65b9\u6cd5\uff0c\u548c\u4e0b\u6e38\u61c9\u7528: \u5982\u679c\u67d3\u5291 (\u00a0stain ) \u4e2d\u5305\u62ec\u56fa\u5b9a\u5291 (fixing agent)\uff0c\u4f8b\u5982\u7532\u9187 (methanol) \u548c\u4e59\u9178 (acetic acid )\uff0c\u5efa\u8b70\u4f7f\u7528\u542b0.1-0.5\uff05SDS (0.1-0.5% SDS) \u6216\u9178\u4e0d\u7a69\u5b9a\u8868\u9762\u6d3b\u6027\u5291 (acid labile surfactants) \u7684\u6d17\u812b\u7de9\u885d\u6db2(elution buffers) ; \u00a0\u5982\u679c\u51dd\u81a0\u662f\u672a\u67d3\u8272\u6216\u8ca0\u67d3 (negatively stained) \u7684\u86cb\u767d\u8cea\u53ef\u7528ddH 2 O\u6216\u542b\u6709\u7532\u9178\/\u6c34\/ 2-\u4e19\u9187\uff081\uff1a3\uff1a2v \/ v \/ v\uff09\u4e4b\u6d17\u812b\u7de9\u885d\u6db2 (elution buffer )\u3002Elution\u7684\u9ad4\u7a4d\u572810\u81f3200\u03bcL\u4e4b\u9593\u3002\u591a\u500b\u51dd\u81a0\u584a\u53ef\u4ee5\u5728\u55ae\u500b\u7ba1\u4e2d\u8655\u7406\uff0c\u4e26\u4e14\u6700\u7d42\u86cb\u767d\u8cea\u6fc3\u5ea6\u53ef\u76f8\u5c0d\u63d0\u9ad8\u3002\u63d0\u53d6\u7684\u86cb\u767d\u8cea\u53ef\u7528\u65bcMALD-MS\u5206\u6790 (MALD-MS analysis) \u3001\u52d5\u7269\u514d\u75ab(immunization )\u3001\u86cb\u767d\u8cea – \u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528 (protein-protein interaction) \u548c\u86cb\u767d\u8cea – \u6838\u9178\u76f8\u4e92 (protein-nucleic acid interaction )\u4f5c\u7528\u7814\u7a76\u7b49\u3002\u6838\u9178\u53ef\u7528\u65bcPCR\uff0cCloning\u548csequencing\u7b49\u3002<\/p>\n

\"\"<\/p>\n

 <\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc. Extracted nucleic acid can be used for PCR, cloning, and sequencing etc.<\/p>\n

\"\"<\/p>\n

 <\/p>\n

\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n
Item<\/td>\nAmount<\/td>\n<\/tr>\n
Filter Cartridges<\/td>\n20<\/td>\n<\/tr>\n
Microfuge Tube<\/td>\n20<\/td>\n<\/tr>\n
Extraction Powder<\/td>\n2g<\/td>\n<\/tr>\n
Micro-pestles<\/td>\n5<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1<\/strong> <\/span>\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |\u81a0\u9ad4\u86cb\u767d\u8cea\u53ca\u6838\u9178<\/strong>\u8403\u53d6\u5957\u7d44<\/strong><\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Minute\u2122\u81a0\u9ad4\u86cb\u767d\u8cea\u53ca\u6838\u9178\u8403\u53d6\u5957\u7d44 | Minute\u2122 Protein\/Nucleic Acid Extr […]<\/p>\n","protected":false},"author":3,"featured_media":7536,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[4247],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7535"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7535"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7535\/revisions"}],"predecessor-version":[{"id":50499,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7535\/revisions\/50499"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7536"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7535"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7535"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7535"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}