{"id":738,"date":"2014-02-06T15:31:52","date_gmt":"2014-02-06T07:31:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=738"},"modified":"2025-01-20T15:51:30","modified_gmt":"2025-01-20T07:51:30","slug":"safeview","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=738","title":{"rendered":"SafeView\u2122 \u6838\u9178\u5b89\u5168\u67d3\u5291 |ABM good\u8ca8\u865f#G108\/G108-G\u53ef\u66ff\u4ee3EtBr\u7684\u6838\u9178\u67d3\u5291"},"content":{"rendered":"<p style=\"text-align: justify;\">\u3000\u3000SafeView\u2122\u70ba\u4e00\u53ef\u66ff\u4ee3EtBr\u7684\u6838\u9178\u67d3\u5291\uff0c\u70ba\u65b0\u4e00\u4ee3\u5b89\u5168\u6027\u87a2\u5149\u67d3\u5291\uff0c\u9748\u654f\u5ea6\u8207EtBr\u76f8\u7576\u3002SafeView\u2122\u7cfb\u5217\u7522\u54c1\u7d93Ames-test\u6aa2\u6e2c\uff0c\u4e0d\u5177\u81f4\u764c\u6027\u3002Agarose\u6216polyacrylamide gel\u7686\u53ef\u4f7f\u7528\u3002<\/p>\n<p style=\"text-align: justify;\"><img decoding=\"async\" src=\"https:\/\/www.ibiantech.com\/wp-content\/uploads\/2017\/03\/safegreen-4-01.jpg\" alt=\"\u67e5\u770b\u4f86\u6e90\u5716\u7247\" width=\"1043\" data-reactid=\"28\" data-bm=\"9\" \/><\/p>\n<p><span style=\"color: #008000; font-size: medium;\"><strong>\u61c9\u7528<\/strong><\/span><\/p>\n<p style=\"padding-left: 30px;\">In gel staining\u3001Gel extraction\u3001Ligation\u3001Transformation\u3001Transfection\u7b49\u5be6\u9a57\u3002<\/p>\n<p><span style=\"color: #008000; font-size: medium;\"><b style=\"line-height: 1.5em;\">SafeView<sup>TM<\/sup><\/b><b style=\"line-height: 1.5em;\">\u7cfb\u5217\u7522\u54c1\uff1a<\/b><\/span><\/p>\n<ul>\n<li><strong>SafeView\u2122\u00a0\uff0dEtBr\u66ff\u4ee3\u54c1\u3002\u4f7f\u7528\u65b9\u6cd5\u8207EtBr\u985e\u4f3c\uff0c\u7121\u9808\u66f4\u6539\u4f7f\u7528\u7fd2\u6163\uff0c\u53ef\u4f7f\u7528\u85cd\u5149\/UV\u5075\u6e2c\u3002<\/strong><\/li>\n<\/ul>\n<p style=\"padding-left: 30px;\">\uff0dSafeView\u2122 Classic\uff0d\u4f5c\u70ba<strong>\u5167\u67d3<\/strong>\u4f7f\u7528\u3002<span style=\"line-height: 1.5em;\">dsDNA\u8207ssDNA\u53ef\u5448\u73fe\u7da0\u8272\u87a2\u5149\u3001RNA\u5448\u73fe\u7d05\u8272\u87a2\u5149\u3002<\/span><br \/>\n\uff0dSafeView\u2122 FireRed\uff0d\u4f5c\u70ba<strong>\u5167\u67d3<\/strong>\u4f7f\u7528\u3002dsDNA\u3001ssDNA\u3001\u4ee5\u53caRNA\u5448\u73fe\u7d05\u8272\u87a2\u5149\u3002<br \/>\n\uff0dSafeView Plus\u2122\uff0d\u4f5c\u70ba<strong>\u5916\u67d3<\/strong>\u4f7f\u7528\u3002<span style=\"line-height: 1.5em;\">\u4e0d\u9700\u9000\u67d3\u6b65\u9a5f\u3002\u9748\u654f\u5ea6\u66f4\u4f73\u3002<\/span><\/p>\n<ul>\n<li><strong>SafeView\u2122\u00a0dyes\uff08Safe-Green\u3001Red\u3001White\u3001Pack\u2122\uff09\uff0d\u53d6\u4ee3DNA Loading dye\uff0c\u4e26\u4f7f\u7528UV\u5075\u6e2c\u3002\u3002<\/strong><\/li>\n<\/ul>\n<ol>\n<li style=\"list-style-type: none;\">\n<ol>\n<li>\u53ef\u5075\u6e2cagarose\u53capolyacrylamide gels\u4e2d\u7684dsDNA\u53cassDNA\u3002<\/li>\n<li>\u53ef\u642d\u914d\u4e0d\u540c\u6ce2\u9577\u6ffe\u7247\u9032\u884cDNA\u5075\u6e2c\u3002<\/li>\n<li>Safe-Green\u53ef\u4f7f\u7528\u85cd\u5149\/UV\u5075\u6e2c\u3002<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p><b style=\"color: #008000; font-size: medium; line-height: 1.5em;\">\u4f7f\u7528\u65b9\u5f0f<\/b><\/p>\n<ul>\n<li>SafeView\u2122 Classic\u3001FireRed\uff1a100ml agarose\u6eb6\u6db2\u4e2d\u52a0\u51655\u03bcl SafeView\u2122 \u5f8c\u9032\u884c\u88fd\u81a0\u3002<\/li>\n<li>SafeView Plus\u2122\uff1aDNA\u96fb\u6cf3\u5b8c\u6210\u5f8c\uff0c\u5c07\u81a0\u9ad4\u6ce1\u5165\u542b\u6709SafeView Plus\u2122\u7684\u96fb\u6cf3\u7de9\u885d\u6db2\uff081:10,000\u7a00\u91cb\uff09\uff0c\u5ba4\u6eab\u4e0b\u53cd\u61c910-40\u5206\u9418\u5373\u53ef\u9032\u884c\u5075\u6e2c\u3002<\/li>\n<li>SafeView\u2122 dyes\uff1a\u5c07maker\/\u6a23\u672c\u4e2d\u52a0\u5165SafeView\u2122 dye\uff081:5\u7a00\u91cb\uff09\u5f8c\u9032\u884cDNA\u96fb\u6cf3\u5373\u53ef\u3002<\/li>\n<\/ul>\n<p><span style=\"color: #008000; font-size: medium;\"><strong><span style=\"line-height: 1.5em;\">\u7d50\u679c\u5448\u73fe<\/span><\/strong><\/span><\/p>\n<p>A.\u00a0SafeView\u2122 Classic\uff08\u984f\u8272\u5448\u73fe\u9700\u642d\u914d\u6ffe\u7247\uff09\u3000B\u3001C.\u00a0SafeView\u2122 dyes<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-large wp-image-756\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/02\/safeview_result-1024x323.png\" alt=\"safeview_result\" width=\"1024\" height=\"323\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/02\/safeview_result-1024x323.png 1024w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/02\/safeview_result-300x94.png 300w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/02\/safeview_result.png 1347w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"276\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u6ce2\u9577\uff08Ex\/Em\uff09<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u9748\u654f\u5ea6\uff08ng\uff09<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"676\"><strong>SafeView\u2122 Classic\uff0d\u5167\u67d3<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G108<\/td>\n<td width=\"276\">SafeView\u2122 Classic<\/td>\n<td style=\"text-align: center;\" width=\"100\">\n<p>490nm\/ 635nm<\/p>\n<p>490nm\/520nm<\/td>\n<td style=\"text-align: center;\" width=\"100\">0.1\uff0d0.3<\/td>\n<td style=\"text-align: center;\" width=\"100\">1ml<\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"676\"><strong>SafeView Plus\u2122\uff0d\u5916\u67d3<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G468<\/td>\n<td width=\"276\">SafeView Plus\u2122<\/td>\n<td style=\"text-align: center;\" width=\"100\">290nm\/520nm<\/td>\n<td style=\"text-align: center;\" width=\"100\">0.05\uff0d0.1<\/td>\n<td style=\"text-align: center;\" width=\"100\">1ml<\/td>\n<\/tr>\n<tr>\n<td colspan=\"5\" width=\"676\"><strong>SafeView\u2122 dyes<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G108-G<\/td>\n<td width=\"276\">Safe-Green\u2122<\/td>\n<td style=\"text-align: center;\" width=\"100\">490nm\/525nm<\/td>\n<td style=\"text-align: center;\" width=\"100\">0.2\uff0d0.6<\/td>\n<td style=\"text-align: center;\" width=\"100\">1ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G108-R<\/td>\n<td width=\"276\">Safe-Red\u2122<\/td>\n<td style=\"text-align: center;\" width=\"100\">490nm\/630nm<\/td>\n<td style=\"text-align: center;\" width=\"100\">0.3\uff0d0.8<\/td>\n<td style=\"text-align: center;\" width=\"100\">1ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G108-W<\/td>\n<td width=\"276\">Safe-White\u2122<\/td>\n<td style=\"text-align: center;\" width=\"100\">370nm\/470nm<\/td>\n<td style=\"text-align: center;\" width=\"100\">0.2\uff0d0.5<\/td>\n<td style=\"text-align: center;\" width=\"100\">1ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G108-P<\/td>\n<td width=\"276\">Safe-Pack\u2122<\/td>\n<td colspan=\"3\" width=\"300\">\u53c3\u7167G108-G\u3001R\u3001W<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2017\/09\/tag2-e1505358209440-300x79.jpg\" alt=\"HOT\" width=\"104\" height=\"27\" \/><strong>\u00a0<span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/wp-admin\/post.php?post=5337&amp;action=edit\">\u9ef4\u6f3f\u83cc\u6c61\u67d3<\/a>\u00a0|<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?news=annexin-v-%E7%B4%B0%E8%83%9E%E5%87%8B%E4%BA%A1%E7%B3%BB%E5%88%97%E5%95%86%E5%93%81-%E6%AA%A2%E6%B8%ACapoptosis%E6%97%A9%E6%9C%9F%E7%B4%B0%E8%83%9E%E8%86%9C%E8%AE%8A%E5%8C%96\">\u00a0\u7d30\u80de\u51cb\u4ea1<\/a>Annexin-V\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?news=cell-damage-oxidative-stress%E7%B4%B0%E8%83%9E%E6%90%8D%E5%82%B7%E8%88%87%E6%B0%A7%E5%8C%96%E5%A3%93%E5%8A%9B%E7%B3%BB%E5%88%97%E5%95%86%E5%93%81\">\u6297\u6c27\u5316\u5206\u6790<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?news=%E3%80%90%E9%A3%9F%E5%AE%89%E6%AA%A2%E6%B8%AC%E3%80%91%E6%97%A5%E6%9C%AC%E9%BE%9C%E7%94%B2%E8%90%ACkikkoman-pd-30-%E6%89%8B%E6%8C%81%E5%BC%8F-atp-%E5%BE%AE%E7%94%9F%E7%89%A9%E5%86%B7%E5%85%89%E5%84%80\">\u98df\u5b89\u6aa2\u6e2c<\/a> (\u5fae\u751f\u7269\u6aa2\u6e2c)\u00a0<\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000SafeView\u2122\u70ba\u4e00\u53ef\u66ff\u4ee3EtBr\u7684\u6838\u9178\u67d3\u5291\uff0c\u70ba\u65b0\u4e00\u4ee3\u5b89\u5168\u6027\u87a2\u5149\u67d3\u5291\uff0c\u9748\u654f\u5ea6\u8207EtBr\u76f8\u7576\u3002SafeVi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":739,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[473,88,132,95,3],"tags":[4841],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/738"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=738"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/738\/revisions"}],"predecessor-version":[{"id":50580,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/738\/revisions\/50580"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/739"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=738"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=738"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=738"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}