{"id":7379,"date":"2016-11-02T16:47:39","date_gmt":"2016-11-02T08:47:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7379"},"modified":"2022-07-25T14:41:42","modified_gmt":"2022-07-25T06:41:42","slug":"protein-free-blocking-buffer%e9%ab%98%e8%83%8c%e6%99%af%e5%b0%88%e7%94%a8blocking-%e8%b2%a8%e8%99%9fjk92-w003","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7379","title":{"rendered":"Protein Free Blocking Buffer|\u9ad8\u80cc\u666f\u5c08\u7528blocking \u8ca8\u865f#JK92-W003"},"content":{"rendered":"

\u9ad8\u80cc\u666f\u5c08\u7528blocking\u00a0 | \u8ca8\u865fJK92-W003<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39:\u00a0<\/strong>Protein Free Blocking Buffer\uff1a<\/strong>\u4e0d\u542b\u86cb\u767d\u6210\u4efd\uff0c\u9069\u7528\u65bc\u80cc\u666f\u8f03\u9ad8\u7684sample or blotting\u3002\u53ef\u61c9\u7528\u65bc<\/span>\uff1aWestern blot\u3001ELISA\u3001Protein array\u7b49\u5be6\u9a57\u3002\u6240\u9700\u6642\u9593\uff1a15-30min\u3002\u50b3\u7d71\u4e0a\u4f7f\u7528\u812b\u8102\u725b\u5976\u6216BSA\u6eb6\u6db2\uff0c\u963b\u65b7\u975e\u7279\u7570\u6027\u6297\u539f\u7d50\u5408\u4f4d\u3002\u5e38\u61c9\u7528\u65bcWB\u3001ELISA\u3001IHC\u7b49\u5206\u6790\u3002T-Pro\u00a0Protein free\u963b\u65b7\u7de9\u885d\u6db2(protein free blocking buffer) \u542b\u6709\u5c08\u6709\u5316\u5408\u7269\uff0c\u53ef\u61c9\u7528\u65bc\u5728ELISA\u3001Western\u5370\u8de1\u3001\u9663\u5217\u548c\u5176\u4ed6\u514d\u75ab\u5316\u5b78\u61c9\u7528\u4e2d\uff1b\u963b\u65b7\u904e\u91cf\u7d50\u5408\u4f4d\u9ede\u3002 \u8a72\u963b\u65b7\u7de9\u885d\u6db2\u6e1b\u5c11\u6216\u6d88\u9664\u4e86\u50b3\u7d71\u86cb\u767d\u8cea\u963b\u65b7\u8a66\u5291\u9047\u5230\u7684\u8a31\u591a\u554f\u984c\uff0c\u4f8b\u5982\u4ea4\u53c9\u53cd\u61c9\u6027\u548c\u4f86\u81ea\u7cd6\u57fa\u5316\u7684\u5e72\u64fe\u3002 \u6b64\u5916\uff0cT-Pro\u86cb\u767d\u8cea\u81ea\u7531\u963b\u65b7\u7de9\u885d\u6db2\u8207\u6297\u9ad4\u548c\u6297\u751f\u7269\u7d20\u86cb\u767d\/\u751f\u7269\u7d20\u7cfb\u7d71\u517c\u5bb9\u3002<\/span><\/p>\n

\u7522\u54c1\u7a2e\u985e<\/strong><\/span><\/p>\n

Protein Free Blocking Buffer\uff1a<\/strong>\u4e0d\u542b\u86cb\u767d\u6210\u4efd\uff0c\u9069\u7528\u65bc\u80cc\u666f\u8f03\u9ad8\u7684sample or blotting\u3002<\/span><\/p>\n

\u7528\u9014\uff1aWestern blot\u3001ELISA\u3001Protein array\u7b49\u5be6\u9a57\u3002<\/p>\n

\u6240\u9700\u6642\u9593\uff1a15-30min\u3002<\/p>\n

Fast Blocking Buffer\uff1a<\/strong>\u6240\u9700\u6642\u9593\u77ed\u3002\u53ef\u505a\u70ba\u6297\u9ad4\u914d\u7f6e\u6db2\uff0c\u5927\u5e45\u6e1b\u5c11blocking\u6642\u9593\u3002<\/span><\/p>\n

\u7528\u9014\uff1aWestern blot\u3002\u6240\u9700\u6642\u9593\uff1a5-10min\u3002<\/p>\n

\u4f7f\u7528\u65b9\u6cd5<\/b><\/span><\/p>\n

    \n
      \n
    1. \u53d6\u51faBlotting membrane\uff0c\u5c07transfer buffer \u6d17\u4e7e\u6de8\u3002<\/li>\n
    2. \u52a0\u5165Blocking buffer\uff0c\u6df9\u904emembrane\u5f8c\u5747\u52fb\u6416\u64fa\u6df7\u5408\u3002<\/li>\n
    3. \u52a0\u5165\u914d\u88fd\u597d\u7684\u6297\u9ad4\u6db2\uff0c\u9032\u884c\u5f8c\u7e8c\u6b65\u9a5f\u3002<\/li>\n<\/ol>\n<\/ol>\n\n\n\n\n\n\n\n
      \n

      \u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n

      		\n

      \u7522\u54c1\u540d\u7a31<\/strong><\/p>\n<\/td>\n

      		\n

      \u5305\u88dd<\/strong><\/p>\n<\/td>\n<\/tr>\n

      JK92-W001<\/td>\nFast Blocking Buffer (in PBS)<\/td>\n500ml<\/td>\n<\/tr>\n
      JK92-W002<\/td>\nFast Blocking Buffer (in TBS)<\/td>\n500ml<\/td>\n<\/tr>\n
      JK92-W003<\/td>\nProtein Free Blocking Buffer (in PBS)<\/td>\n500ml<\/td>\n<\/tr>\n
      JK92-W004<\/td>\nProtein Free Blocking Buffer (in TBS)<\/td>\n500ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      \u9ad8\u80cc\u666f\u5c08\u7528blocking\u00a0 | \u8ca8\u865fJK92-W003 \u7522\u54c1\u4ecb\u7d39:\u00a0Protein Free Blockin […]<\/p>\n","protected":false},"author":3,"featured_media":7382,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89],"tags":[4294],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7379"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7379"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7379\/revisions"}],"predecessor-version":[{"id":15292,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7379\/revisions\/15292"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7382"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7379"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7379"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}