{"id":7262,"date":"2016-10-26T13:38:13","date_gmt":"2016-10-26T05:38:13","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7262"},"modified":"2023-09-25T11:46:35","modified_gmt":"2023-09-25T03:46:35","slug":"atp%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-atp-colorimetricfluorometric-assay-kit-%e8%b2%a8%e8%99%9fk354-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7262","title":{"rendered":"ATP\u5448\u8272\u5206\u6790\u5957\u7d44 | ATP Colorimetric\/Fluorometric Assay Kit \u8ca8\u865fK354-100 (Abcam ab83355)"},"content":{"rendered":"

\u5448\u8272\u87a2\u5149\u5206\u6790!!<\/span>\u00a0\u00a0<\/strong>\u00a0<\/strong>ATP<\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong>ATP Colorimetric\/Fluorometric Assay Kit | \u8ca8\u865fK354-100<\/strong><\/a>\u00a0Abcam ab83355<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39: Biovision <\/strong>\u63d0\u4f9b\u4e00\u7cfb\u5217<\/strong>ADP\/ATP<\/strong>\u51b7\u5149\u53ca\u5316\u5b78\u5448\u8272\u5206\u6790\u5957\u7d44\uff1b\u5176\u4e2d<\/strong>ATP<\/strong>\u5448\u8272\u87a2\u5149<\/strong>\u5206\u6790\u5957\u7d44<\/strong>\uff1b\u5176\u5229\u7528\u7518\u6cb9\u7684\u78f7\u9178\u5316\u7522\u751f\uff0c\u53ef\u4ee5\u5448\u8272\uff08\u03bbmax = 570nm\uff09\u6216\u87a2\u5149\u6e2c\u5b9a\uff08Ex \/ Em = 535\/587 nm\uff09\u5169\u7a2e\u65b9\u6cd5\u6e2c\u91cfATP\u4e4b\u542b\u91cf\u3002<\/p>\n

\"\"<\/p>\n

ATP\u662f\u751f\u547d\u7cfb\u7d71\u7684\u4e3b\u8981\u80fd\u91cf\u3002ATP\u50c5\u5728\u7c92\u7dda\u9ad4\u4e2d\u5f62\u6210\uff0c\u591a\u7a2e\u907a\u50b3\u75be\u75c5\u53ef\u5f71\u97ff\u7dda\u7c92\u9ad4\u4e2d\u7684ATP\u5f62\u6210\u3002ATP\u6e2c\u5b9a\uff08\u4f8b\u5982BioVision Kit 254-200\uff09\u6aa2\u6e2c\u98db\u79d2\u4ee5\u4e0b\u7684ATP\uff0c\u9019\u4e9b\u8a66\u5291\u76d2\u9700\u8981\u5177\u6709\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100\u5668\u3002BioVision\u65b0\u958b\u767c\u7684ATP\u6bd4\u8272\u548c\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2\u8a2d\u8a08\uff0c\u63d0\u4f9b\u7c21\u55ae\u7684\u65b9\u6cd5\uff0c\u4ee5\u5448\u8272\uff08\u03bbmax = 570nm\uff09\u6216\u87a2\u5149\u6e2c\u5b9a\uff08Ex \/ Em = 535\/587 nm\uff09\u65b9\u6cd5\u3002 \u8a72\u6e2c\u5b9a\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u4f4e\u81f350 picomol\u03bcM\u7684ATP\u542b\u91cf\u3002<\/p>\n

\"ATP<\/p>\n

Maiti AK et al (2018) used ATP assay kit ab83355 to measure mitochondrial ATP generation in an in vitro<\/em>\u00a0mouse intestinal model treated with cytokines in the presence and absence of VIP (vasoactive intestinal peptide). VIP was induced by\u00a0C. rodentium<\/em>\u00a0infection and cytokines.<\/p>\n

\"ATP<\/p>\n

he chart shows a comparison of ATP levels of HepG2 treated with\u00a00, 10 and 100 \u00b5M for 48 hours,\u00a0DRH2O2 W1\u00a0<\/sup>(damage recovered cells using hydrogen peroxide with a recovery time of one week) HepG2 cells and MDA-MB-231 cells treated with 0, 10 and 100 \u00b5M of NaHS for 48 hours. Data is shown as percent of ATP levels in untreated cells.\u00a0ATP levels were determined using ATP assay kit (ab83355).<\/p>\n

\"ATP<\/p>\n

MCF-7 cells\u00a0are transfected with vector, osteopontin-a, osteopontin-c or osteopontin -a\u00a0plus -c. Cells are plated on poly-HEMA and seeded at 4 x 105\u00a0<\/sup>cells per well and incubated for two days under standard culture conditions. ATP levels are measured using ATP assay kit (ab83355)<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
\u51b7\u5149\u5448\u8272\u5206\u6790<\/strong><\/td>\n<\/tr>\n
\u00a0K255-200<\/td>\n\u00a0ApoSENSOR\u2122 ADP\/ATP Ratio Bioluminescence Assay Kit<\/td>\n200 assays<\/td>\n<\/tr>\n
\u00a0K254-200
\nK254-1000<\/td>\n		\u00a0ApoSENSOR\u2122 ATP Cell Viability Bioluminescence Assay Kit<\/td>\n200 assays
\n1000 assays<\/td>\n<\/tr>\n
\u00a0K791-100
\nK791-1000<\/td>\n		\u00a0StayBrite\u2122 Highly Stable ATP Bioluminescence Assay Kit\u2014\u9577\u6548\u578b\u51b7\u5149ATP\u5206\u6790<\/td>\n100 assays
\n1000 assays<\/td>\n<\/tr>\n
\u5316\u5b78\u5448\u8272\u5206\u6790<\/strong><\/td>\n<\/tr>\n
\u00a0K356-100<\/td>\n\u00a0ADP Colorimetric Assay Kit II<\/td>\n100 assays<\/td>\n<\/tr>\n
\u00a0K355-100<\/td>\n\u00a0ADP Colorimetric\/Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
\u00a0K354-100<\/td>\n\u00a0ATP Colorimetric\/Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

ab83355 has been referenced in 198 publications.<\/span><\/p>\n

\n
Takasu, C. et al., (2017) Treatment with dimethyl fumarate ameliorates liver ischemia\/reperfusion injury,\u00a0World J Gastroenterol, Oct.2017, 23(25): 4508\u20134516.<\/a><\/div>\n<\/div>\n
\n
Wu J et al., (2017) Redox imbalance and mitochondrial abnormalities in the diabetic lung,\u00a0Redox Biol, 2017, 11:51-59<\/a><\/div>\n<\/div>\n
\n
Johnstone M et al., (2017) Characterization of the Pro-Inflammatory Cytokine IL-1\u03b2 on Butyrate Oxidation in Colorectal Cancer Cells,\u00a0J Cellular Biochem, 2017, 118(6):1614-1621<\/a><\/div>\n<\/div>\n
\n
Andres-Hernando, Ana et al. (2017) Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice,\u00a0Nat Commun. 2017 Feb 13;8:14181.<\/a><\/div>\n<\/div>\n
\n
Rahn et al., Zebrafish lacking functional DNA polymerase gamma survive to juvenile stage, despite rapid and sustained mitochondrial DNA depletion, altered energetics and growth.\u00a0\u00a0Nucleic Acids Res.,\u00a0Oct 2015; 10.1093\/nar\/gkv1139.<\/a><\/div>\n<\/div>\n
\"\u540c\u8cea\u5546\u54c17\"<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"

\u5448\u8272\u87a2\u5149\u5206\u6790!!\u00a0\u00a0\u00a0ATP\u5448\u8272\u5206\u6790\u5957\u7d44ATP Colorimetric\/Fluorometric Assay […]<\/p>\n","protected":false},"author":3,"featured_media":6976,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,3],"tags":[4319],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7262"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7262"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7262\/revisions"}],"predecessor-version":[{"id":45819,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7262\/revisions\/45819"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6976"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7262"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7262"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7262"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}