{"id":7101,"date":"2016-10-14T16:35:26","date_gmt":"2016-10-14T08:35:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7101"},"modified":"2025-12-31T10:21:15","modified_gmt":"2025-12-31T02:21:15","slug":"sod%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-superoxide-dismutase-sod-activity-assay%e8%b2%a8%e8%99%9fk335-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7101","title":{"rendered":"SOD\u6d3b\u6027\u5206\u6790\u5957\u7d44 | Superoxide Dismutase (SOD) Activity Assay\u8ca8\u865fK335-100 Abcam ab65354"},"content":{"rendered":"

Superoxide Dismutase (SOD) <\/strong>\u6d3b\u6027\u5206\u6790\u5957\u7d44 |\u00a0<\/strong>\u8ca8\u865fK335-100\u00a0<\/a>\u00a0\u00a0K335\u5408\u4f75\u70baAbcam ab65354<\/span><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

 <\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u6c27\u5316\u76f8\u95dc\u5206\u6790\u5957\u7d44\uff1b\u5176\u4e2dSOD\u6d3b\u6027\u5206\u6790\u5957\u7d44\uff0c\u53ef\u9078\u64c7\u5438\u6536\u5149Absorbance (450 nm)\u5075\u6e2c\uff1b\u53ef\u7528\u65bc\u7d30\u80de\u7d44\u7e54supernatants\u3001\u5c3f\u6db2\u3001\u8840\u6f3f\u3001\u6216\u8840\u6e05\u4e4b\u6db2\u9ad4\u578b\u5f0f\u7684\u6a23\u54c1\u4f86\u6e90\u3002\u64cd\u4f5c\u6642\u9593\u5927\u7d0430\u5206\u9418\u3002\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u662f\u6700\u91cd\u8981\u7684\u6297\u6c27\u5316\u9176 (antioxidative enzymes)\u4e4b\u4e00\u3002 \u5b83\u50ac\u5316\u8d85\u6c27\u9670\u96e2\u5b50(\u00a0superoxide anion)\u5206\u89e3\u70ba\u904e\u6c27\u5316\u6c2b(\u00a0hydrogen peroxide\u00a0)\u548c\u5206\u5b50\u6c27(molecular oxygen)\u3002SOD\u6e2c\u5b9a\u8a66\u5291\u76d2\u5229\u7528WST-1\uff0c\u5176\u5728\u7528\u8d85\u6c27\u5316\u7269\u9670\u96e2\u5b50(\u00a0superoxide anion)\u9084\u539f\u6642\u7522\u751f\u6c34\u6eb6\u6027\u67d3\u6599\u3002 \u8d85\u6c27\u9670\u96e2\u5b50\u9084\u539f\u7684\u901f\u7387\u8207\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08\u00a0xanthine oxidase, XO\uff09\u6d3b\u6027\u7dda\u6027\u76f8\u95dc\uff0c\u4e26\u4e14\u88abSOD\u6291\u5236\u3002 SOD\u7684\u6291\u5236\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u5448\u8272\u6cd5\u6e2c\u5b9a\u3002<\/p>\n

\"Functional<\/p>\n

Superoxide dismutase (SOD) is one of the most important . It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. The sensitive SOD assay kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD (below). Therefore, the inhibition activity of SOD can be determined by a colorimetric method.<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n

\"Biochemical<\/p>\n

\u76f8\u95dc\u7522\u54c1<\/span> \u89f8\u9176Catalase\u6d3b\u6027\u5206\u6790<\/a> \uff5c \u9aa8\u9ad3\u904e\u6c27\u5316\u9176(Myeloperoxidase) \u5448\u8272\u5206\u6790<\/a> \uff5cHydrogen Peroxide\u5206\u6790\u5957\u7d44 <\/a>\u00a0| \u7e3d\u6297\u6c27\u5316\u7269\u5448\u8272\u5206\u6790\u5957\u7d44<\/a><\/span><\/p>\n

Superoxide Dismutase (SOD)<\/strong>\u6d3b\u6027<\/strong>\u5206\u6790\u5957\u7d44 |<\/strong> K335-100<\/strong>\u5167\u5bb9\u7269(Abcam ab65354)<\/strong>
\n\u2022 WST Solution
\n\u2022 SOD Enzyme Solution
\n\u2022 SOD Assay Buffer
\n\u2022 SOD Dilution Buffer<\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/th>\nK335-100(Abcam ab65354)<\/td>\n<\/tr>\n
Size<\/th>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/th>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Applications<\/th>\nKit contains the necessary reagents for convenient measurement of activity of Superoxide Dismutase (SOD) by colorimetric method.<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 Simple one-step procedure; takes around than 30 minutes
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 WST Solution
\n\u2022 SOD Enzyme Solution
\n\u2022 SOD Assay Buffer
\n\u2022 SOD Dilution Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
<\/div>\n
\"K335\"<\/a><\/strong><\/div>\n
<\/div>\n
Madhusmita Dhupal, et al., ( 2018) Immunotoxicity of titanium dioxide nanoparticles via simultaneous induction of apoptosis and multiple toll-like receptors signaling through ROS-dependent SAPK\/JNK and p38 MAPK activation,\u00a0International journal of Nanomedicine, Oct. 2018, 30425486<\/a><\/div>\n<\/div>\n
\n
Jingsi Zhang, et al., (2018) Jia-Jian-Di-Huang-Yin-Zi decoction exerts neuroprotective effects on dopaminergic neurons and their microenvironment,\u00a0Scientific Reports, Jun. 2018, 29959371<\/a><\/div>\n<\/div>\n
\n
Lin, CH. et al., (2017) Activation of AMPK is neuroprotective in the oxidative stress by advanced glycosylation end products in human neural stem cells,\u00a0Experimental Cell Research, Oct.2017, 359( 2): 367-373<\/a><\/div>\n<\/div>\n
\n
Baysal, M et al., (2017) Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress,\u00a0PLoS One, 2017, 12(4): e0175990<\/a><\/div>\n<\/div>\n
\n
Ga, Yoon Na et al. (2017) The relationship between circulating neutrophil gelatinase-associated lipocalin and early alteration of metabolic parameters is associated with dietary saturated fat intake in non-diabetic Korean women,\u00a0Endocr J. 2017 Mar 31;64(3):303-314.<\/a><\/div>\n
\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"<\/div>\n
<\/div>\n
\"smart\"<\/a>\"Spectrumlab<\/a>\"\u71b1\u92b7\u5546\u54c13\"\"0100-01\"<\/a><\/div>\n
<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K671-100<\/td>\n\u00a0Ascorbic Acid Colorimetric Assay Kit II (FRASC)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K661-100<\/td>\n\u00a0Ascorbic Acid Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K773-100<\/td>\n\u00a0Catalase Activity Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K620-100<\/td>\n\u00a0Ethanol Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K504-100<\/td>\n\u00a0EZCell\u2122 Glutathione Detection Kit (Blue Fluorescence)\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K207-100<\/td>\n\u00a0EZCell\u2122 Intracellular Nitric Oxide Synthase (NOS) Detection Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K264-100<\/td>\n\u00a0Glutathione (GSH\/GSSG\/Total) Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K261-100<\/td>\n\u00a0Glutathione Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K251-100<\/td>\n\u00a0Glutathione Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K762-100<\/td>\n\u00a0Glutathione Peroxidase Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K761-200<\/td>\n\u00a0Glutathione Reductase Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K263-100<\/td>\n\u00a0GST Colorimetric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K260-100<\/td>\n\u00a0GST Fluorometric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K336-100<\/td>\n\u00a0HAT (H4) Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K334-100<\/td>\n\u00a0HAT Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K332-100<\/td>\n\u00a0HAT Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K331-100<\/td>\n\u00a0HDAC Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K330-100<\/td>\n\u00a0HDAC Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K340-100<\/td>\n\u00a0HDAC Inhibitor Drug Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K265-200<\/td>\n\u00a0Hydrogen Peroxide Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K712-100<\/td>\n\u00a0Inosine Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K339-100<\/td>\n\u00a0InSitu HDAC Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K204-200<\/td>\n\u00a0Intracellular Hydrogen Peroxide Detection Kit (cell-based)<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K739-100<\/td>\n\u00a0Lipid Peroxidation (MDA) Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K744-100<\/td>\n\u00a0Myeloperoxidase (MPO) Colorimetric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K745-100<\/td>\n\u00a0Myeloperoxidase (MPO) Fluorometric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K746-200<\/td>\n\u00a0Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K747-100<\/td>\n\u00a0Myeloperoxidase (MPO) Peroxidation Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K262-200<\/td>\n\u00a0Nitric Oxide Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K252-200<\/td>\n\u00a0Nitric Oxide Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K205-100<\/td>\n\u00a0Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric) <\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K206-100<\/td>\n\u00a0Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K208-100<\/td>\n\u00a0Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4926-100<\/td>\n\u00a0NQO1 (human intracellular) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K772-100<\/td>\n\u00a0Peroxidase Activity Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K840-100<\/td>\n\u00a0Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K335-100<\/td>\n\u00a0Superoxide Dismutase (SOD) Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K763-100<\/td>\n\u00a0Thioredoxin Reductase Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K274-100<\/td>\n\u00a0Total Antioxidant Capacity (TAC) Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K608-100<\/td>\n\u00a0Uric Acid Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K710-100<\/td>\n\u00a0Xanthine Oxidase Activity Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>\u4ee3\u8b1d\u76f8\u95dc\u5546\u54c1\u00a0<\/strong><\/span>\u78b3\u6c34\u5316\u5408\u7269\u548c\u8102\u8cea\u4ee3\u8b1d<\/a> | \u80fa\u57fa\u9178\u8207\u7121\u6a5f\u96e2\u5b50\u4ee3\u8b1d<\/a> | \u7cd6\u9175\u89e3\u9014\u5f91\u548c\u4e2d\u4ecb\u4ee3\u8b1d<\/a>\u00a0|\u6c27\u5316\u50b7\u5bb3\u8207\u8840\u6e05\u6210\u5206\u5206\u6790<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Superoxide Dismutase (SOD) \u6d3b\u6027\u5206\u6790\u5957\u7d44 |\u00a0\u8ca8\u865fK335-100\u00a0\u00a0\u00a0K335\u5408\u4f75 […]<\/p>\n","protected":false},"author":3,"featured_media":6324,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,517],"tags":[4350],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7101"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7101"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7101\/revisions"}],"predecessor-version":[{"id":54329,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7101\/revisions\/54329"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6324"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7101"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7101"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7101"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}