{"id":7068,"date":"2016-10-13T14:14:44","date_gmt":"2016-10-13T06:14:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7068"},"modified":"2022-07-25T16:22:19","modified_gmt":"2022-07-25T08:22:19","slug":"divaa%e6%b4%bb%e9%ab%94%e8%a1%80%e7%ae%a1%e6%96%b0%e7%94%9f%e5%a5%97%e7%b5%84-trevigen%e8%b2%a8%e8%99%9f3450-048-sk","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7068","title":{"rendered":"DIVAA\u6d3b\u9ad4\u8840\u7ba1\u65b0\u751f\u5957\u7d44 | Trevigen\u8ca8\u865f3450-048-SK"},"content":{"rendered":"<p style=\"text-align: left;\"><strong>Trevigen\u00a0Directed In Vivo Angiogenesis Assay\uff08DIVAA\uff09\u6d3b\u9ad4\u8840\u7ba1\u65b0\u751f\u8a66\u9a57\u5957\u7d44 | \u8ca8\u865f3450-048-SK<\/strong><\/p>\n<p>\u53d6\u4ee3\u50b3\u7d71 plug assay\u81ea\u9ad4\u5438\u6536\u554f\u984c\uff1b\u5e02\u552e\u5b9a\u91cf\u4e14\u5177\u91cd\u8986\u6027\u7684invivo\u8840\u7ba1\u65b0\u751f\u5957\u7d44<\/p>\n<p>\u6d3b\u9ad4\u65b0\u8840\u7ba1\u8a66\u9a57\u5957\u7d44DIVAA\uff0c\u662f\u5e02\u9762\u4e0a\u552f\u4e00\u53ef\u4ee5\u5b9a\u91cf\u4e14\u5177\u91cd\u8986\u6027\u7684\u6d3b\u9ad4\u5167\u8840\u7ba1\u65b0\u751f\u5957\u7d44\u3002\u53d6\u4ee3\u50b3\u7d71Plug assay\u81ea\u9ad4\u5438\u6536\u554f\u984c\u3002\u6b64\u5957\u7d44\u5229\u7528\u8840\u7ba1\u65b0\u751f\u8a66\u9a57\u7ba1(angioreactor)\uff0c\u5c07\u5f85\u6e2c\u85e5\u7269\u8207BME\u6df7\u5408\u7269\u586b\u81f3\u8a66\u9a57\u7ba1\uff0c\u4e26\u690d\u5165\u8001\u9f20\u9ad4\u5167\u9032\u884c\u8840\u7ba1\u65b0\u751f\u8a66\u9a57\u3002\u8207\u50b3\u7d71\u7684Matrigel plug\u65b9\u6cd5\u76f8\u6bd4\uff0cBME\u6df7\u5408\u7269\u4e0d\u6703\u88ab\u81ea\u9ad4\u5438\u6536\u3002\u56e0\u6b64\uff0c\u9664\u4e86\u5b9a\u6027\u5916\u4ea6\u53ef\u540c\u6642\u5b9a\u91cf\u8840\u7ba1\u65b0\u751f\u7684\u7a0b\u5ea6\uff0c\u8a66\u9a57\u7d50\u679c\u518d\u73fe\u6027\u9ad8\u3001\u4eba\u70ba\u8aa4\u5dee\u7a0b\u5ea6\u5c0f\u7b49\u512a\u9ede\u662f\u505a\u6d3b\u9ad4\u8840\u7ba1\u65b0\u751f\u7684\u6700\u4f73\u9078\u64c7\u3002\u5be6\u9a57\u7d50\u679c\u5df2\u520a\u767b\u5728Cell\u3001American J Pathol\u3001J Biol Chem\u3001Blood\u7b49\u671f\u520a\uff0c\u7686\u7372\u5f97\u826f\u597d\u56de\u61c9\u3002<img decoding=\"async\" loading=\"lazy\" class=\"aligncenter\" title=\"\u827e\u7f8e\u6377\u63a8\u8350Cultrex\u00ae DIVAA\u4f53\u5185\u8840\u7ba1\u5f62\u6210\u5206\u6790\u7cfb\u7edf\" src=\"http:\/\/www.bio-review.com\/wp-content\/uploads\/2012\/03\/divaa-system.jpg\" alt=\"\u827e\u7f8e\u6377\u63a8\u8350Cultrex\u00ae DIVAA\u4f53\u5185\u8840\u7ba1\u5f62\u6210\u5206\u6790\u7cfb\u7edf\" width=\"420\" height=\"256\" \/><strong>\u8840\u7ba1\u65b0\u751f\u4ecb\u7d39<\/strong><strong>:<\/strong> \u8840\u7ba1\u751f\u6210\uff08Angiogenesis)\u662f\u6307\u7531\u5df2\u5b58\u5728\u7684\u8840\u7ba1\u7db2\u7684\u5167\u76ae\u7d30\u80de\u5206\u5316\u800c\u5f62\u6210\u65b0\u7684\u8840\u7ba1\u3002\u8840\u7ba1\u751f\u6210\u904e\u7a0b\uff1a\u5305\u62ec\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u7684\u9077\u79fb\u548c\u589e\u6b96\u3001\u8840\u7ba1 \u57fa\u5e95\u819c\u7684\u964d\u89e3\u3001\u86cb\u767d\u6eb6\u89e3\u9176\u7684\u8868\u9054\u53ca\u5176\u5c0d\u7d30\u80de\u5916\u57fa\u8cea\u7684\u964d\u89e3\u3001\u65b0\u5f62\u6210\u7d30\u80de\u5916\u57fa\u8cea\u7684\u91cd\u6392\u548c\u5167\u76ae\u7d30\u80de\u8840\u7ba1\u58c1\u7684\u5f62\u6210\u3002\u8840\u7ba1\u751f\u6210\u7684\u904e\u7a0b\u5305\u62ec\u4e86\uff0c\u5728\u5408\u9069\u7684\u689d\u4ef6\u4e0b\uff0c\u5167\u76ae\u7d30\u80de\u901a\u904e\u81ea\u6211\u7d44\u88dd\u80fd\u5f62\u6210\u5fae\u8840\u7ba1\u6216\u5c0f\u7ba1\u72c0\u7d50\u69cb\u3002\u6297\u8840\u7ba1\u751f\u6210\u5df2\u7d93\u6210\u70ba\u6cbb\u7642\u816b\u7624\u8f49\u79fb\u3001\u7cd6\u5c3f\u75c5\u8996\u7db2\u819c\u75c5\u8b8a\u3001\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e\u7b49\u75be\u75c5\u7684\u91cd\u8981\u7b56\u7565\u4e4b\u4e00\u3002<\/p>\n<p><strong>Trevigen<\/strong><strong>\u5c08\u5229\u7684DIVAA\u7522\u54c1<\/strong>: \u662f\u4e16\u754c\u4e0a\u7b2c\u4e00\u500b\u7528\u65bc\u9ad4\u5167\u5b9a\u91cf\u6aa2\u6e2c\u8840\u7ba1\u767c\u751f\u7684\u6aa2\u6e2c\u7cfb\u7d71\uff0c\u5be6\u9a57\u5177\u6709\u53ef\u91cd\u8907\u6027\u3002\u5c07\u542b\u670920 \u03bcl Trevigen&#8217;s basement membrane extract (BME)\u7684\u53ef\u79fb\u690d\u77fd\u6a39\u8102\u67f1\uff08\u8840\u7ba1\u53cd\u61c9\u5668\u3001angioreactors\uff0c\u4e26\u6839\u64da\u7814\u7a76\u9700\u8981\u52a0\u5165\u6216\u4e0d\u52a0\u5165\u8840\u7ba1\u751f\u6210\u8abf\u7bc0\u56e0\u6578\uff09\uff0c\u690d\u5165\u88f8\u9f20\u76ae\u4e0b\u3002\u79fb\u690d\u5f8c9\u5929\uff0c\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u9577\u5165BME\uff0c\u4e26\u751f\u6210\u8840\u7ba1\u3002\u7814\u7a76\u4eba\u54e1\u53ef\u6839\u64da\u5be6\u9a57\u9700\u8981\uff0c\u5728BME\u4e2d\u52a0\u5165\u4e0d\u540c\u7684\u8840\u7ba1\u751f\u6210\u8abf\u7bc0\u56e0\u6578\uff08\u4fc3\u9032\u6216\u6291\u5236\u8840\u7ba1\u767c\u751f\u56e0\u6578\uff1a\u5982FGF-2\u3001VEGF\u3001MMP2\u3001 adrenomedullin\u3001TIMP-2\u548cCD97\u7b49\uff09\u89c0\u5bdf\u5176\u5c0d\u9ad4\u5167\u8840\u7ba1\u767c\u751f\u4f5c\u7528\u7684\u5f71\u97ff\u3002DIVAA\u53ef\u70ba\u9ad4\u5167\u8840\u7ba1\u751f\u6210\u7684\u53ef\u518d\u751f\u548c\u53ef\u5b9a\u91cf\u5206\u6790\u63d0\u4f9b\u6a19\u6e96\u5316\u6aa2\u6e2c\u65b9\u6848\u3002\u8840\u7ba1\u53cd\u61c9\u5668\u907f\u514d\u4e86\u7531\u65bc\u5c0f\u9f20\u57fa\u5e95\u819c\u63d0\u53d6\u7269\u5438\u6536\u503c\u7522\u751f\u7684\u932f\u8aa4\u3002\u6b64\u5916\uff0c\u8840\u7ba1\u53cd\u61c9\u5668\u50c5\u9700\u5c11\u91cf\u6750\u6599\uff0c\u7bc0\u7701\u4e86BME\u548c\u6e2c\u8a66\u5316\u5408\u7269\u7684\u4f7f\u7528\uff0c\u6bcf\u53ea\u5c0f\u9f20\u9ad4\u5167\u53ef\u4ee5 \u690d\u5165\u591a\u90544\u500b\u53cd\u61c9\u5668\uff0c\u63d0\u4f9b\u7d71\u8a08\u610f\u7fa9\u3002<\/p>\n<p><strong>Cultrex\u00ae DIVAA Starter Kit<\/strong>\u8a66\u5291\u76d2<\/p>\n<p>\u5167\u5305\u542b\u4e8648\u500b\u8840\u7ba1\u53cd\u61c9\u5668\uff08\u4e00\u7a2e\u53ef\u5728\u9ad4\u5167\u79fb\u690d\u7684\u77fd\u6a39\u8102 \u67f1\uff09\uff0c\u4ee5\u53caTrevigen\u5c08\u5229\u7684BME\u57fa\u8cea\uff08\u5177\u6709\u6975\u4f4e\u7684\u7d30\u80de\u6bd2\u6027\uff0c\u7d93\u56b4\u683c\u7684\u7121\u7d30\u83cc\/\u771f\u83cc\/\u652f\u539f\u9ad4\u7b49\u9a57\u8b49\uff0c\u5167\u6bd2\u7d20\u542b\u91cf\u6975\u4f4e\uff09\uff0c\u4ee5\u53ca\u8840\u7ba1\u751f\u6210\u6240\u9700\u7684\u5fc5\u8981\u751f\u9577 \u56e0\u6578\u548c\u5176\u5b83\u8a66\u5291\uff0c\u53ef\u9032\u884c48\u6b21\u5206\u6790\u3002\u5728\u8d77\u59cb\u8a66\u5291\u76d2\u5167\u9084\u5305\u62ec\u4e86\u7368\u7279\u7684Angiorack\u652f\u67b6\u8a2d\u8a08\uff0c\u65b9\u4fbf\u5728\u521d\u59cb\u5b89\u88dd\u6642\u5c0d\u8840\u7ba1\u53cd\u61c9\u5668\u7684\u5b9a\u4f4d\u56fa\u5b9a\u3002<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td width=\"110\"><strong>Catalog #<\/strong><\/td>\n<td><strong>Component \/ Size<\/strong><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=240\">3450-048-01<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=240\">DIVAA Angioreactor \/ 48 reactors<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=241\">3450-048-02<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=241\">DIVAA BME \/ 6 x 200uL<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=242\">3450-048-03<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=242\">DIVAA Wash Buffer \/ 25 ml<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=243\">3450-048-04<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=243\">DIVAA FGF-2 Positive Control \/ 100 ng\/10 uL<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=244\">3450-048-05<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=244\">DIVAA Cell Sperse \/ 15 ml<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=246\">3450-048-08<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=246\">Heparin Solution \/ 10 \u03bcl 2mg\/ml<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=247\">3450-048-09<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=247\">AngioRack \/ 48 positions<\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=248\">3450-048-B10<\/a><\/td>\n<td><a href=\"https:\/\/www.trevigen.com\/itemComponent.php?itemsKey=248\">DIVAA FGF-2 (1.8 \u00aeg)\/VEGF(600 ng) \/ 60 \u03bcl<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><span style=\"text-align: justify; color: #008000;\">\u512a\u9ede\uff1a<\/span><\/strong><\/p>\n<ul>\n<li>\u7531\u7f8e\u570b\u570b\u5bb6\u885b\u751f\u7814\u7a76\u9662\uff08NIH\uff09William Stetler-Stevenson, MD, Ph.D.\u6240\u7814\u767c\u3002<\/li>\n<li>Cultrex<sup>\u00ae<\/sup> BME \u6fc3\u5ea6\u9ad8\u65bcMatrigel\uff0c\u5728\u9ad4\u5167\u5be6\u9a57\u6548\u679c\u66f4\u70ba\u986f\u8457\u3002<\/li>\n<li>\u5229\u7528\u8a66\u9a57\u7ba1\u5305\u8986Cultrex<sup>\u00ae<\/sup> BME\u4fbf\u65bc\u690d\u5165\uff0c\u4f5c\u70baPlug Assay\u7684\u53e6\u4e00\u7a2e\u9078\u64c7\u3002<\/li>\n<li>\u9ad4\u7a4d\u56fa\u5b9a\u4fbf\u65bc\u5b9a\u91cf\uff0c\u518d\u73fe\u6027\u9ad8\u3002<\/li>\n<li>\u8001\u9f20\u9ad4\u5167\u6700\u591a\u53ef\u690d\u5165\u56db\u652f\u8a66\u9a57\u7ba1\uff0c\u6bcf\u652f\u8a66\u9a57\u7ba1\u50c5\u970020 \u03bcl\u6a23\u54c1\u3002<\/li>\n<\/ul>\n<div id=\"attachment_2195\" style=\"width: 530px\" class=\"wp-caption aligncenter\"><img aria-describedby=\"caption-attachment-2195\" decoding=\"async\" loading=\"lazy\" class=\" wp-image-2195\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Trevigen_DIVAA_operation.gif\" alt=\"Trevigen_DIVAA_operation\" width=\"520\" height=\"185\" \/><p id=\"caption-attachment-2195\" class=\"wp-caption-text\">1. Angioreactor\u690d\u5165\u6d3b\u9ad4\u4e2d\u3002 2. Angioreactor\u53d6\u51fa\u793a\u610f\u5716 \u30023. \u5176\u4e2dBME\u7684\u8840\u7ba1\u65b0\u751f\u73fe\u8c61\u3002<\/p><\/div>\n<table class=\"alignleft\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\"><strong>\u7522\u54c1\u578b\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3450-048-SK<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae DIVAA Starter Kit<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">48 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3450-048-K<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae DIVAA Activation Kit<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">48 Wells<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3450-048-IK<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae DIVAA Inhibition Kit<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">48 Wells<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3450-048-DA<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae DIVAA Angioreactor and BME<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">48 Wells<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3450-048-FL<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae DIVAA FITC-Lectin &amp; Buffer<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">48 Wells<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" valign=\"top\" width=\"568\">\n<p style=\"text-align: center;\" align=\"center\"><span style=\"color: #333333;\"><strong>Cultrex\u00ae Angiogenesis\u76f8\u95dc\u7522\u54c1<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3470-096-K<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae In Vitro Angiogenesis Assay Tube Formation Kit<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">96 Wells<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" valign=\"top\" width=\"100\">3471-096-K<\/td>\n<td valign=\"top\" width=\"375\">Cultrex\u00ae In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit<\/td>\n<td style=\"text-align: center;\" valign=\"top\" width=\"81\">96 tests<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Trevigen\u00a0Directed In Vivo Angiogenesis Assay\uff08DIVAA\uff09\u6d3b\u9ad4\u8840\u7ba1 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2189,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[125],"tags":[4358],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7068"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7068"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7068\/revisions"}],"predecessor-version":[{"id":7077,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7068\/revisions\/7077"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2189"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7068"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7068"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7068"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}