{"id":7031,"date":"2016-10-12T11:49:50","date_gmt":"2016-10-12T03:49:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7031"},"modified":"2024-05-13T17:48:38","modified_gmt":"2024-05-13T09:48:38","slug":"i%e5%9e%8b%e8%86%a0%e5%8e%9ftype-i-collagen-applied-cell-extracellular-matrix%e8%b2%a8%e8%99%9fg422","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7031","title":{"rendered":"I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix\u8ca8\u865fG422"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7031\"><strong><span style=\"font-size: 12pt;\"><span style=\"color: #33cccc;\">A<\/span><\/span><\/strong><span style=\"font-size: 12pt; color: #33cccc;\"><strong>bmgood<\/strong><strong>\u7d30\u80de\u57f9\u990a\u5546\u54c1<\/strong> <strong>|Applied Cell Extracellular Matrix<\/strong><strong>\u8ca8\u865f<\/strong><strong>G422 <\/strong><strong>|<\/strong><strong>\u5305\u88dd<\/strong><strong>25ml<\/strong><\/span><\/a><\/span><\/p>\n<p>\u5728\u7d30\u80de\u751f\u7269\u5b78\u4e2d\uff0c\u7d93\u7531\u7d30\u80de\u7c98\u9644(cell adhesion)\u523a\u6fc0\uff0c\u5f71\u97ff\u7d30\u80de\u5206\u5316(cell differentiation)\u3001\u7d30\u80de\u9031\u671f(cell cycle)\u3001\u7d30\u80de\u5b58\u6d3b(cell cycle)\u7b49signal\u3002 \u56e0\u6b64\uff0cadhesion\u5728in vitro\u9ad4\u5916\u7814\u7a76\u4e2d\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002ABM\u7d30\u80de\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix)\u4e3b\u8981\u7531\u5f9e\u5927\u9f20\u5c3e\u8171(rat tail tendon)\u5206\u96e2\u7684I\u578b\u81a0\u539f\u7d44\u6210(type I collagen)\u3002I\u578b\u81a0\u539f(type I collagen)\u662f\u81a0\u539f\u7684\u6700\u8c50\u5bcc\u7684\u5f62\u5f0f\uff0c\u800c\u8207\u5176\u5b83\u81a0\u539f\u6709\u6240\u4e0d\u540c\uff0c\u975e\u4f4e\u8cf4\u6c28\u9178\u7fa5\u5316\u548c\u4f4e\u78b3\u6c34\u5316\u5408\u7269\u6240\u7d44\u6210\u3002\u7d30\u80de\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix)\u9069\u7528\u65bc\u56fa\u9ad4\u51dd\u81a0\u6216cultrure dish\u7684\u5857\u5c64\uff1b\u5176\u7279\u9ede\u70ba\u589e\u5f37\u7d30\u80de\u8cbc\u9644\uff0c\u53ef\u61c9\u7528\u65bc\u5ee3\u6cdb\u7684\u7d30\u80de\u682a\uff0c\u4f8b\u5982\u809d\u7d30\u80de(hepatocytes)\uff0c\u6210\u7e96\u7dad\u7d30\u80de(fibroblasts)\u548c\u4e0a\u76ae\u7d30\u80de(epithelial cells)\u4e2d\u7684\u9644\u8457\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/G422.jpg\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u7522\u54c1\u7279\u9ede<\/strong><\/span><\/p>\n<p>-\u7121\u83cc\u578b\u5f0f\u5177\u9ad8\u7d14\u5ea6 4mg\/ml of pure collagen (&gt;95%).<br \/>\n-stored in 20 mM acetic acid (pH ~3.0).<br \/>\n-direct addition to culture wares for coating<br \/>\n-simple and rapid plate coating procedure.<br \/>\n-enhance cell attachment in a variety of primary cells.<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u4f7f\u7528\u65b9\u6cd5\u53ca\u7528\u91cf<\/strong><\/span><\/p>\n<p>1. \u5728\u751f\u7269\u5b89\u5168\u6ac3\u4e2d\uff0c\u5c07\u6240\u9700\u9ad4\u7a4d\u7684Applied Cell\u7d30\u80de\u5916\u57fa\u8cea\u7f6e\u65bc\u5f85\u5305\u8986\u7684\u677f\u5b54\u6216\u57f9\u990a\u76bf\u7684\u8868\u9762\u4e0a\uff1b\u78ba\u4fdd\u5b54\/\u57f9\u990a\u76bf\u7684\u6574\u500b\u5e95\u90e8\u90fd\u88ab\u6eb6\u6db2\u8986\u84cb\u3002\u8acb\u53c3\u95b1\u8868 1 \u4ee5\u4e86\u89e3\u9ad4\u7a4d\u6307\u5357\u3002<br \/>\n2. \u5728\u751f\u7269\u5b89\u5168\u6ac3\u4e2d\u6253\u958b\u84cb\u5b50\uff0c\u5728\u5ba4\u6eab\u4e0b\u57f9\u990a 1 \u5c0f\u6642\u3002<br \/>\n3. \u5f9e\u677f\u5b54\u6216\u57f9\u990a\u76bf\u4e2d\u5438\u51fa\u6eb6\u6db2\u3002\u5c07\u677f\u5b54\u6216\u57f9\u990a\u76bf\u518d\u653e\u7f6e 1 \u5c0f\u6642\uff0c\u8b93\u8868\u9762\u4e7e\u71e5\u3002<br \/>\n4. \u5857\u5c64\u8868\u9762\u53ef\u7acb\u5373\u4f7f\u7528\uff0c\u6216\u5728 4 &#8211; 8<sup>oC<\/sup> \u4e0b\u4fdd\u5b58\u9577\u9054 2 \u9031\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/05\/2-I-%E5%9E%8B%E8%86%A0%E5%8E%9Ftype-I-collagen-Applied-Cell-Extracellular-Matrix%E8%B2%A8%E8%99%9FG422.png\" width=\"788\" height=\"186\" \/><\/p>\n<p><strong><span style=\"color: #800000; font-size: 12pt;\">Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1\u00a0(immortalized reagenet)\u00a0Ready-to-use<\/span><\/strong><\/p>\n<p>Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n<p><strong><span style=\"color: #ff0000;\">HOT!!<\/span><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5545\"> \u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5582\">DAPI\u67d3\u6838<\/a> | <\/span><\/strong><span style=\"color: #3366ff;\"><strong><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5564\">\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a><\/strong><strong> | <\/strong><strong><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5567\">\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a><\/strong><strong> | <\/strong><strong><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5575\">\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Abmgood\u7d30\u80de\u57f9\u990a\u5546\u54c1 |Applied Cell Extracellular Matrix\u8ca8\u865fG422  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":7032,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453,458],"tags":[4368],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7031"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7031"}],"version-history":[{"count":9,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7031\/revisions"}],"predecessor-version":[{"id":49423,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7031\/revisions\/49423"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7032"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7031"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7031"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7031"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}