{"id":7027,"date":"2016-10-12T10:56:19","date_gmt":"2016-10-12T02:56:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7027"},"modified":"2023-06-20T16:40:05","modified_gmt":"2023-06-20T08:40:05","slug":"%e4%b8%8d%e6%9c%bd%e5%b0%8f%e8%86%a0%e8%b3%aa%e7%b4%b0%e8%83%9e-sv40-large-t-human-microglia-sv40%e8%b2%a8%e8%99%9f-t0251","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7027","title":{"rendered":"\u4e0d\u673d\u5c0f\u81a0\u8cea\u7d30\u80de-SV40 Large T | Human Microglia – SV40\u8ca8\u865f T0251"},"content":{"rendered":"

A<\/strong>bmgood\u4e0d\u673d<\/strong>\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)-Immortalized Human Microglia<\/strong><\/span><\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong> |\u8ca8\u865f T0251 1×106<\/sup> cells \/ 1.0 ml<\/strong><\/a><\/span><\/p>\n

\u4e0d\u673d<\/strong>\u5c0f\u81a0\u8cea\u7d30\u80de\u00a0 |\u8ca8\u865fT0251 |\u5305\u88dd1×106<\/sup>\u00a0cells \/ 1.0 ml \u00a0Immortalized Human Microglia – SV40<\/p>\n

\u5c0f\u81a0\u8cea\u7d30\u80de(Microglia cells)\u662f\u5927\u8166\u548c\u810a\u9ad3\u7684\u5e38\u99d0\u5de8\u566c\u7d30\u80de\uff0c\u5b83\u5011\u662f\u795e\u7d93\u7cfb\u7d71\u4e2d\u4e3b\u52d5\u514d\u75ab\u9632\u79a6\u7684\u7b2c\u4e00\u7a2e\u4e5f\u662f\u4e3b\u8981\u5f62\u5f0f\u3002\u6c38\u751f\u5316\u4eba\u5c0f\u81a0\u8cea\u7d30\u80de\u4fc2\u4f86\u6e90\u65bc\u539f\u4ee3\u4eba\u5c0f\u81a0\u8cea\u7d30\u80de\uff08>99% \u7d14\u5ea6\uff09\uff0c\u4e26\u4fdd\u7559\u4e86\u5c0f\u81a0\u8cea\u7d30\u80de\u6a19\u8a18\u7269 CD68 \u548c NGF\u3002\u9019\u4e9b\u7d30\u80de\u9069\u7528\u65bc\u7814\u7a76\u5065\u5eb7\u548c\u75be\u75c5\u4e2d\u7684\u4eba\u985e\u5c0f\u81a0\u8cea\u7d30\u80de( human microglia )\u3002<\/p>\n

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\u5c0f\u81a0\u8cea\u7d30\u80deImmortalized Human Microglia\u7d30\u80de\u682a | Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen | \u4f86\u6e90Brain \u3002Microglia cells\u5c0f\u81a0\u8cea\u7d30\u80de\u662f\u5927\u8166\u548c\u810a\u9ad3\u5de8\u566c\u7d30\u80de\uff0c\u5728\u795e\u7d93\u7cfb\u7d71\u4e2d\u4e3b\u8981\u7684\u514d\u75ab\u9632\u79a6\u5f62\u5f0f\u3002\u4e0d\u673d\u4e4b\u4eba\u5c0f\u81a0\u8cea\u7d30\u80de – SV40\u7d30\u80de\u682a\uff0c\u6e90\u81ea\u521d\u4ee3\u4eba\u985e\u5c0f\u795e\u7d93\u81a0\u8cea\u7d30\u80de\uff08> 99\uff05\u7d14\u5ea6\uff09\uff0c\u4e26\u4e14\u901a\u904eRT-PCR\u8b49\u5be6\u4fdd\u7559\u5c0f\u81a0\u8cea\u7d30\u80de\u7279\u7570\u6027\u6a19\u8a18\u7269\uff1b\u4f8b\u5982NGF\uff0cCD68\u548cTREM2\u3002Expression Profile: NGF, Iba1, TREM2, CD11b, CD68, Sall1, CD45\u3002\u9019\u4e9b\u7d30\u80de\u63d0\u4f9b\u4eba\u985e\u5c0f\u81a0\u8cea\u7d30\u80de\u7684\u5065\u5eb7\u548c\u75be\u75c5\u4e4b\u7814\u7a76\u6a21\u5f0f\u3002<\/p>\n

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Microglia cells\u5c0f\u81a0\u8cea\u7d30\u80de\u57f9\u990a\u689d\u4ef6 Use of PriCoat\u2122 T25 Flasks (G299<\/a>) or Applied Cell Extracellular Matrix (G422<\/a>) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.<\/strong>\u00a0The base medium for this cell line is Prigrow III medium available in ABM, Cat. No.TM003<\/a>. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and 1% Penicillin\/Streptomycin (G255<\/a>). This cell line needs to be cultured in collagen coated flasks. Atmosphere: air, 95%; Carbon dioxide (CO2<\/sub>), 5%. Temperature: 37.0\u00b0C.<\/p>\n

\u5fc5\u9700\u8cfc\u8cb7PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u57f9\u990a\u6db2PriGrow III (TM003)\u3002<\/p>\n

\u00a0\"\"<\/div>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002<\/p>\n

\"cell-biology-tab-final<\/p>\n

\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong><\/span><\/p>\n

\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002 Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0<\/strong>(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106<\/sup>\u53ca109<\/sup>\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

ABM<\/strong>\u63d0\u4f9b\u4ee5\u4e0b\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u5de5\u5177\uff1a<\/strong><\/p>\n