{"id":7010,"date":"2016-10-11T14:44:55","date_gmt":"2016-10-11T06:44:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7010"},"modified":"2023-03-13T12:47:57","modified_gmt":"2023-03-13T04:47:57","slug":"gpx%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-glutathione-peroxidase-assay-%e8%b2%a8%e8%99%9fk762-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7010","title":{"rendered":"GPx\u5448\u8272\u5206\u6790\u5957\u7d44 |Glutathione Peroxidase Assay \u8ca8\u865fK762-100 (Abcam ab102530)"},"content":{"rendered":"

\u00a0GPx\u5448\u8272\u5206\u6790\u5957\u7d44Glutathione Peroxidase Activity Colorimetric Assay Kit<\/span><\/a>\u00a0 \u00a0<\/span><\/span>\u8ca8\u865fK762-100\u00a0 (Abcam ab102530)<\/span><\/p>\n

0.5 mU\/ml<\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u6c27\u5316\u76f8\u95dc\u5206\u6790\u5957\u7d44\uff1b\u5176\u4e2dGPx\u5448\u8272\u5206\u6790\u5957\u7d44\uff0c\u4ee5\u5448\u8272Colorimetric \u00a0(340 nm)\u5075\u6e2c\uff1b\u53ef\u7528\u65bc\u7d30\u80de\u7d44\u7e54lysate\u7d30\u80de\u57f9\u990a\u6db2\u3001\u5c3f\u6db2\u3001\u8840\u6f3f\u3001\u6db2\u9ad4\u578b\u5f0f\u7684\u4f86\u6e90\u3002\u64cd\u4f5c\u6642\u9593\u5927\u7d0430-40\u5206\u9418\u3002\u9748\u654f\u5ea60.5 mU\/ml of GPx in samples<\/p>\n

\"\"<\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k788 \u03bb= 570 nm\uff09<\/span><\/p>\n

GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\uff08Glutathione Peroxidase, GPx\uff0cEC 1.11.1.9\uff09\u5728\u4fdd\u8b77\u751f\u7269\u514d\u53d7\u6c27\u5316\u640d\u50b7(oxidative damage)\u4e2d\uff0c\u626e\u6f14\u91cd\u8981\u4f5c\u7528\u3002 GPx\uff08Glutathione Peroxidase, GPx\uff09\u5c07\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione, GSH\uff09\u8f49\u5316\u70ba\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08oxidized glutathione , GSSG\uff09\uff0c\u540c\u6642\u5c07\u8102\u8cea\u6c2b\u904e\u6c27\u5316\u7269(\u00a0lipid hydroperoxides) \u9084\u539f\u70ba\u5176\u76f8\u61c9\u7684\u9187(alcohols)\u6216\u5c07\u6e38\u96e2\u904e\u6c27\u5316\u6c2b(free hydrogen peroxide )\u9084\u539f\u70ba\u6c34\u3002 \u5df2\u7d93\u5728\u4e0d\u540c\u7684\u7d30\u80de\u4f4d\u7f6e\u548c\u4e0d\u540c\u7684\u5e95\u7269\u7279\u7570\u6027\u4e2d\u767c\u73fe\u4e86\u5e7e\u7a2e\u540c\u529f\u9176\u3002 \u4f4e\u6c34\u5e73\u7684GPx\u5df2\u7d93\u8207\u81ea\u7531\u57fa\u76f8\u95dc\u7684\u75be\u75c5\u76f8\u95dc(free radical related disorders)\u3002 \u5728BioVision\u7684\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176(Glutathione Peroxidase, GPx)\u6d3b\u6027\u6e2c\u5b9a\u4e2d\uff0cGPx (Glutathione Peroxidase, GPx)\u9084\u539f\u67af\u70ef\u6c2b\u904e\u6c27\u5316\u7269(Cumene Hydroperoxid)\uff0c\u540c\u6642\u5c07GSH\uff08reduced glutathione, GSH\uff09\u6c27\u5316\u6210GSSG\uff08oxidized glutathione , GSSG\uff09\u3002 \u6240\u7522\u751f\u7684GSSG\uff08oxidized glutathione , GSSG\uff09\u88ab\u9084\u539f\u70baGSH\uff08reduced glutathione, GSH\uff09\uff0c\u901a\u904eGR (glutathione reductase) \u6d88\u8017NADPH\u3002 NADPH\u7684\u964d\u4f4e\uff08\u5728340nm\u5bb9\u6613\u6e2c\u91cf\uff09\u8207GPx(Glutathione Peroxidase, GPx)\u6d3b\u6027\u6210\u6bd4\u4f8b\u3002 \u8a72\u6e2c\u5b9a\u53ef\u7528\u65bc\u6e2c\u91cf\u8840\u6f3f\uff0c\u7d05\u7d30\u80de\u88c2\u89e3\u7269\uff0c\u7d44\u7e54\u52fb\u6f3f\u548c\u7d30\u80de\u88c2\u89e3\u7269\u4e2d\u7684\u6240\u6709\u7a40\u80f1\u7518\u80bd\u4f9d\u8cf4\u6027\u904e\u6c27\u5316\u7269\u9176\uff0c\u6a23\u54c1\u4e2d\u7684GPx(Glutathione Peroxidase, GPx)\u7684\u6aa2\u6e2c\u9748\u654f\u5ea6\u70ba\u301c0.5mU \/ ml\u3002<\/p>\n

\"GSH_GSSG\"<\/p>\n

GPx<\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44 | K762-100<\/strong>\u5167\u5bb9\u7269<\/strong>
\n\u2022 GPx Assay Buffer
\n\u2022 NADPH (lyophilized)
\n\u2022 Glutathione Reductase
\n\u2022 Glutathione (GSH, lyophilized)
\n\u2022 Cumene Hydroperoxide
\n\u2022 GPx Positive Control (lyophilized);<\/p>\n

\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"<\/p>\n

\n
Zhou Zhou, Adipose\u2010Specific Lipin\u20101 Overexpression Renders Hepatic Ferroptosis and Exacerbates Alcoholic Steatohepatitis in Mice.\u00a0Hepatol Commun, May 2019; 31061954.<\/a><\/div>\n<\/div>\n
\n
Long Chen, LncRNA GAS5 regulates redox balance and dysregulates the cell cycle and apoptosis in malignant melanoma cells.\u00a0J Cancer Res Clin Oncol, Dec 2018; 30569211.<\/a><\/div>\n<\/div>\n
\n
Jesmin Ara, et al., (2018) Hydrogen Water Drinking Exerts Antifatigue Effects in Chronic Forced Swimming Mice via Antioxidative and Anti-Inflammatory Activities,\u00a0BioMed Research International, Apr. 2018, 29850492<\/a><\/div>\n<\/div>\n
\n
Musabayane, C. T. et al., (2017) Kidney Function in P Berghei-Infected Sprague-Dawley Rats Following Treatment with Transdermally Delivered Syzygium-aromaticum Derived Oleanolic Acid,\u00a0J Endocrinology and Thyroid Res, Apr.2017, 1(3)<\/a><\/div>\n<\/div>\n
\n
Sibiya, HP et al., (2017) Kidney Function in P. Berghei-Infected Sprague-Dawley Rats Following Treatment with Transdermally Delivered Syzygium-aromaticum Derived Oleanolic Acid,\u00a0J Endocrinol Thyroid Res, 2017, 1(3): 555565<\/a><\/div>\n
<\/div>\n
\"K762\"<\/a><\/div>\n
<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K671-100<\/td>\n\u00a0Ascorbic Acid Colorimetric Assay Kit II (FRASC)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K661-100<\/td>\n\u00a0Ascorbic Acid Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K773-100<\/td>\n\u00a0Catalase Activity Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K620-100<\/td>\n\u00a0Ethanol Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K504-100<\/td>\n\u00a0EZCell\u2122 Glutathione Detection Kit (Blue Fluorescence)\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K207-100<\/td>\n\u00a0EZCell\u2122 Intracellular Nitric Oxide Synthase (NOS) Detection Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K264-100<\/td>\n\u00a0Glutathione (GSH\/GSSG\/Total) Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K261-100<\/td>\n\u00a0Glutathione Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K251-100<\/td>\n\u00a0Glutathione Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K762-100<\/td>\n\u00a0Glutathione Peroxidase Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K761-200<\/td>\n\u00a0Glutathione Reductase Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K263-100<\/td>\n\u00a0GST Colorimetric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K260-100<\/td>\n\u00a0GST Fluorometric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K336-100<\/td>\n\u00a0HAT (H4) Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K334-100<\/td>\n\u00a0HAT Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K332-100<\/td>\n\u00a0HAT Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K331-100<\/td>\n\u00a0HDAC Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K330-100<\/td>\n\u00a0HDAC Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K340-100<\/td>\n\u00a0HDAC Inhibitor Drug Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K265-200<\/td>\n\u00a0Hydrogen Peroxide Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K712-100<\/td>\n\u00a0Inosine Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K339-100<\/td>\n\u00a0InSitu HDAC Activity Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K204-200<\/td>\n\u00a0Intracellular Hydrogen Peroxide Detection Kit (cell-based)<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K739-100<\/td>\n\u00a0Lipid Peroxidation (MDA) Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K744-100<\/td>\n\u00a0Myeloperoxidase (MPO) Colorimetric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K745-100<\/td>\n\u00a0Myeloperoxidase (MPO) Fluorometric Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K746-200<\/td>\n\u00a0Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K747-100<\/td>\n\u00a0Myeloperoxidase (MPO) Peroxidation Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K262-200<\/td>\n\u00a0Nitric Oxide Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K252-200<\/td>\n\u00a0Nitric Oxide Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K205-100<\/td>\n\u00a0Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K206-100<\/td>\n\u00a0Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K208-100<\/td>\n\u00a0Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4926-100<\/td>\n\u00a0NQO1 (human intracellular) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K772-100<\/td>\n\u00a0Peroxidase Activity Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K840-100<\/td>\n\u00a0Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K335-100<\/td>\n\u00a0Superoxide Dismutase (SOD) Activity Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K763-100<\/td>\n\u00a0Thioredoxin Reductase Activity Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K274-100<\/td>\n\u00a0Total Antioxidant Capacity (TAC) Colorimetric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K608-100<\/td>\n\u00a0Uric Acid Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K710-100<\/td>\n\u00a0Xanthine Oxidase Activity Colorimetric\/Fluorometric Assay Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n

\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0GPx\u5448\u8272\u5206\u6790\u5957\u7d44Glutathione Peroxidase Activity Colorimetric […]<\/p>\n","protected":false},"author":3,"featured_media":6786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[122],"tags":[4373],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7010"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7010"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7010\/revisions"}],"predecessor-version":[{"id":40008,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7010\/revisions\/40008"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6786"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7010"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7010"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7010"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}