{"id":7008,"date":"2016-10-11T14:09:29","date_gmt":"2016-10-11T06:09:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7008"},"modified":"2022-07-25T16:40:38","modified_gmt":"2022-07-25T08:40:38","slug":"gshgssg%e8%9e%a2%e5%85%89%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-glutathione-gshgssgtotal-%e8%b2%a8%e8%99%9fk264-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=7008","title":{"rendered":"GSH\/GSSG\u87a2\u5149\u5206\u6790\u5957\u7d44 |Glutathione (GSH\/GSSG\/Total) \u8ca8\u865fK264-100"},"content":{"rendered":"

Superoxide Dismutase (SOD)\u00a0<\/strong>\u6d3b\u6027\u5206\u6790<\/strong>\u00a0<\/strong><\/a>|\u00a0Catalase<\/strong>\u00a0<\/strong>\u89f8\u9176\u6d3b\u6027\u5206\u6790<\/strong><\/a>\u00a0|\u00a0\u7e3d\u6297\u6c27\u5316\u7269\u5448\u8272\u5206\u6790<\/strong>\u00a0<\/a>\u00a0|\u00a0\u7a40\u80f1\u7518\u80bd\u67d3\u8272\u6aa2\u6e2c<\/strong><\/a>\u00a0|\u00a0Hydrogen Peroxide Detection Kit<\/strong>\u00a0<\/strong>\u5206\u6790<\/strong><\/a>\u00a0|\u00a0GST<\/strong>\u00a0<\/strong>\u5448\u8272\u5206\u6790<\/strong><\/a><\/span><\/p>\n

GSH\/GSSG<\/strong>\u87a2\u5149\u5206\u6790\u5957\u7d44<\/strong>Glutathione (GSH\/GSSG\/Total) Fluorometric Assay Kit<\/strong> \u00a0 <\/a>\u00a0|\u00a0<\/span><\/span>\u8ca8\u865fK264-100<\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u6c27\u5316\u76f8\u95dc\u5206\u6790\u5957\u7d44\uff1b\u5176\u4e2dGSH\/GSSG\u87a2\u5149\u5206\u6790\u5957\u7d44\uff0c\u4ee5\u87a2\u5149Fluorescence (Ex\/Em 340\/420 nm)\u5075\u6e2c\uff1b\u53ef\u7528\u65bc\u7d30\u80de\u7d44\u7e54lysate\u3001\u7d30\u80de\u57f9\u990a\u6db2\u3001\u5c3f\u6db2\u3001\u8840\u6f3f\u3001\u6216\u8840\u6e05\u4e4b\u6db2\u9ad4\u578b\u5f0f\u7684\u4f86\u6e90\u3002\u64cd\u4f5c\u6642\u9593\u5927\u7d0460-120\u5206\u9418\u3002\u9748\u654f\u5ea62-400 ng\/\u00b5l\uff0c\u53ef\u6e2c\u91cfGSH\u3001GSSG\u3001\u6216total glutathione.<\/p>\n

\"\"<\/p>\n

\"k264\"<\/a><\/p>\n

\u7a40\u80f1\u7518\u80bd (Glutathione ) \u662f\u4e3b\u8981\u7684\u7d30\u80de\u5167\u4f4e\u5206\u5b50\u91cf\u786b\u9187 (\u00a0thiol )\uff0c\u5728\u7d44\u7e54\u548c\u7d30\u80de\u4e2d\u5c0d\u6297\u6c27\u5316\u58d3\u529b (oxidative stress) \u7684\u7d30\u80de\u9632\u79a6\u4e2d\uff0c\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002\u5e02\u552e\u7684\u7a40\u80f1\u7518\u80bd\u6aa2\u6e2c\u8a66\u5291\u76d2\u4f8b\u5982DTNB-\u9176\u5faa\u74b0\u7a40\u80f1\u7518\u80bd\u6e2c\u5b9a\u8a66\u5291\u76d2(DTNB-enzyme cycling glutathione assay kit)\u6216\u57fa\u65bc\u55ae\u6c2f\u806f\u82ef\u7684\u6e2c\u5b9a\u8a66\u5291\u76d2(Monochlorobimane based assay kit)\u5e7e\u4e4e\u7121\u6cd5\u5340\u5206\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08\u00a0reduced glutathione, GSH; FW\uff1a307\uff09\u548c\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08oxidized glutathione , GSSG; FW\uff1a612\uff09\u3002 BioVision\u7684\u7a40\u80f1\u7518\u80bd\u6aa2\u6e2c\u8a66\u5291\u76d2 (BioVision\u2019s Glutathione Detection Kit , K264) \u70ba\u55ae\u7368\u6aa2\u6e2cGSH\uff0cGSSG\u548c\u7e3d\u7a40\u80f1\u7518\u80bd (total glutathione ) \u3002\u63d0\u4f9b\u4e86\u4e00\u500b\u7368\u7279\uff0c\u53ca\u65b9\u4fbf\u7684\u6aa2\u6e2c\u5de5\u5177\u3002\u5728\u6e2c\u5b9a\u4e2d\uff0cOPA\u8207GSH\uff08\u4e0d\u662fGSSG\uff09\u53cd\u61c9\uff0c\u7522\u751f\u87a2\u5149\uff0c\u56e0\u6b64\u53ef\u4ee5\u7279\u7570\u6027\u5730\u5b9a\u91cfGSH\u3002\u6dfb\u52a0\u9084\u539f\u5291\u5c07GSSG\u8f49\u5316\u70baGSH\uff0c\u56e0\u6b64\u53ef\u4ee5\u6e2c\u5b9a\uff08GSH + GSSG\uff09\u3002\u70ba\u4e86\u7279\u7570\u6027\u5730\u6e2c\u91cfGSSG\uff0c\u52a0\u5165GSH quacher\u4ee5\u9664\u53bbGSH\uff0c\u9632\u6b62\u8207OPA\u7684\u53cd\u61c9\uff08\u800cGSSG\u4e0d\u53d7\u5f71\u97ff\uff09\u3002\u7136\u5f8c\u52a0\u5165\u9084\u539f\u5291\u4ee5\u7834\u58de\u904e\u91cf\u7684quacher\u4e26\u5c07GSSG\u8f49\u5316\u70baGSH\u3002\u56e0\u6b64\uff0cGSSG\u53ef\u4ee5\u88ab\u7279\u7570\u6027\u5b9a\u91cf\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u7368\u7279\u7684\u7a0b\u5e8f\u548c\u7de9\u885d\u6db2\u914d\u65b9\uff0c\u4ee5\u6d88\u9664\u86cb\u767d\u8cea\u786b\u9187\u4e4b\u5e72\u64fe\uff0c\u548c\u7a69\u5b9a\u6eb6\u6db2\u4e2d\u7684GSH\u548cGSSG\u3002<\/p>\n

GSH\/GSSG<\/strong>\u87a2\u5149\u5206\u6790\u5957\u7d44 | K264-100<\/strong>\u5167\u5bb9\u7269<\/strong>
\n\u2022 Glutathione Assay Buffer
\n\u2022 PCA (Perchloric Acid, 6N)
\n\u2022 KOH (<6N)
\n\u2022 OPA Probe (o-phthalaldehyde )
\n\u2022 Reducing Agent Mix
\n\u2022 GSH Quencher
\n\u2022 GSH Standard (FW: 307)<\/p>\n

GSH\/GSSG<\/strong>\u87a2\u5149\u5206\u6790\u5957\u7d44<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/th>\nK264-100<\/td>\n<\/tr>\n
Size<\/th>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/th>\nFluorescence (Ex\/Em 340\/420 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/th>\nMammalian<\/td>\n<\/tr>\n
Applications<\/th>\nThe kit provides unique formula and buffers and procedures for detecting the GSH, GSSG, and total glutathione individually<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 Simple procedure; takes only ~1-2 hours
\n\u2022 Fast and convenient
\n\u2022 The assay is easy to perform and detects 2-400 ng\/\u00b5l of GSH, GSSG or total glutathione.<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 Glutathione Assay Buffer
\n\u2022 PCA (Perchloric Acid, 6N)
\n\u2022 KOH (<6N)
\n\u2022 OPA Probe (o-phthalaldehyde )
\n\u2022 Reducing Agent Mix
\n\u2022 GSH Quencher
\n\u2022 GSH Standard (FW: 307)<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4ee3\u8b1d\u76f8\u95dc\u5546\u54c1<\/span>\u00a0<\/strong>\u78b3\u6c34\u5316\u5408\u7269\u548c\u8102\u8cea\u4ee3\u8b1d<\/a> | \u80fa\u57fa\u9178\u8207\u7121\u6a5f\u96e2\u5b50\u4ee3\u8b1d<\/a> | \u7cd6\u9175\u89e3\u9014\u5f91\u548c\u4e2d\u4ecb\u4ee3\u8b1d<\/a>\u00a0|\u6c27\u5316\u50b7\u5bb3\u8207\u8840\u6e05\u6210\u5206\u5206\u6790<\/a><\/span><\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

\n
Lee, Geum-Hwa et al. (2017) Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress,\u00a0BMC Res Notes. 2017 Feb 1;10(1):77.<\/a><\/div>\n<\/div>\n
\n
Charitou et al., FOXOs support the metabolic requirements of normal and tumor cells by promoting IDH1 expression.\u00a0EMBO Rep.,\u00a0Apr 2015; 16: 456 – 466.<\/a><\/div>\n<\/div>\n
\n
Odkhuu et al., Involvement of redox balance in\u00a0in\u00a0vitro<\/em>\u00a0osteoclast formation of RAW 264.7 macrophage cells in response to LPS.Innate Immunity,\u00a0Feb 2015; 21: 194 – 202.<\/a><\/div>\n<\/div>\n
\n
Ishaq et al., Atmospheric gas plasma\u2013induced ROS production activates TNF-ASK1 pathway for the induction of melanoma cancer cell apoptosis.\u00a0Mol. Biol. Cell,\u00a0May 2014; 25: 1523 – 1531.<\/a><\/div>\n<\/div>\n
\n
Chowdhury et al., Disuccinyl Betulin Triggers Metacaspase-Dependent Endonuclease G-Mediated Cell Death in Unicellular Protozoan Parasite\u00a0Leishmania donovani.\u00a0<\/em>Antimicrob. Agents Chemother.,\u00a0Apr 2014; 58: 2186 – 2201.<\/a><\/div>\n<\/div>\n

 <\/p>\n

\u00a0<\/span><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Superoxide Dismutase (SOD)\u00a0\u6d3b\u6027\u5206\u6790\u00a0|\u00a0Catalase\u00a0\u89f8\u9176\u6d3b\u6027\u5206\u6790\u00a0|\u00a0\u7e3d\u6297\u6c27 […]<\/p>\n","protected":false},"author":3,"featured_media":6786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[183,122,518],"tags":[4374],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7008"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7008"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7008\/revisions"}],"predecessor-version":[{"id":29689,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7008\/revisions\/29689"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6786"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7008"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7008"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7008"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}