{"id":657,"date":"2014-10-18T16:53:42","date_gmt":"2014-10-18T08:53:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=657"},"modified":"2022-07-27T16:54:36","modified_gmt":"2022-07-27T08:54:36","slug":"comet_assay","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=657","title":{"rendered":"\u3010DNA damage\u3011Comet assay SCGE \u55ae\u7d30\u80de\u96fb\u6cf3 \/ \u5f57\u661f\u5206\u6790\u2014Trevigen"},"content":{"rendered":"

\"Innova_product_banner01\"\u3000\u3000\u7f8e\u570bTREVIGEN\u516c\u53f8\u6210\u7acb\u65bc1992\u5e74\u3002\u81f4\u529b\u65bc\u7814\u767c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u3001DNA\u65b7\u640d\u8207\u4fee\u5fa9\u3001\u9ad4\u5167\u8840\u7ba1\u65b0\u751f\u5206\u6790\u5957\u7d44\uff08In vivo angiogenesis assay, DIVAA\uff09\u3001\u9ad4\u5916\u8840\u7ba1\u65b0\u751f\u5206\u6790\u5957\u7d44\uff08in vitro angiogenesis assay\uff09\u3001\u6167\u661f\u5206\u6790\u6cd5\uff08Comet assay Kit\uff09\u3001\u7d30\u80de\u79fb\u884c\/\u7d30\u80de\u4fb5\u8972\uff08Cell migration and Cell invasion assay\uff09\u3001\u57fa\u5e95\u819c\u8403\u53d6\u7269\uff08Basement Membrane Extract\uff09\u3001\u4ee5\u53caPAR\u3001PARP\u3001PARG\u6240\u9700\u7528\u5230\u7684\u6297\u9ad4\u8207\u8a66\u5291\u3002<\/span><\/span><\/p>\n

\u3000\u3000\u55ae\u7d30\u80de\u51dd\u81a0\u96fb\u6cf3\uff08Single Cell Gel Electrophoresis Assay\uff09<\/strong><\/span>\u662f\u4e00\u7a2e\u5feb\u901f\u3001 \u7c21\u6613\u6027\u3001\u53ca\u9ad8\u9748\u654f\u5ea6\u6aa2\u6e2c\u55ae\u7368\u7d30\u80deDNA\u53d7\u640d\u7684\u65b9\u6cd5\u3002\u5229\u7528LM agarose\u81a0\u9ad4\u96fb\u6cf3\u5206\u96e2\u4e0d\u540c\u5927\u5c0f\u7247\u6bb5\u7684DNA\uff0c\u5075\u6e2c\u7d30\u80deDNA\u65b7\u88c2\u7684\u60c5\u6cc1\u3002\u65b7\u88c2\u4e4bDNA\u7247\u6bb5\u5728\u96fb\u6cf3\u5f8c\u6703\u79fb\u52d5\u81f3\u7d30\u80de\u6838\u5916\uff0c\u7d93\u7531\u6838\u9178\u67d3\u8272\u5f8c\uff0c\u6703\u5f62\u6210\u62d6\u5c3e\u7684\u73fe\u8c61\uff0c\u6545\u53c8\u540d\u70ba\u5f57\u661f\u5206\u6790\u6cd5\uff08COMET assay\uff09<\/span><\/strong>\uff1b\u82e5\u7d30\u80deDNA\u672a\u640d\u5bb3\u5247\u79fb\u52d5\u6162\uff0c\u4e14\u6703\u7559\u5728\u6838\u8cea\u9ad4\u5167\u3002\u53ef\u85c9\u7531\u5206\u6790DNA\u5206\u5e03\u7684\u60c5\u6cc1\uff0c\u5224\u65b7\u7d30\u80de\u53d7\u640d\u7684\u56b4\u91cd\u5ea6\u3002<\/span><\/p>\n

\u7279\u6027\uff1a<\/strong><\/span><\/p>\n

    \n
      \n
    1. \u4f9d\u4f7f\u7528\u7de9\u885d\u6db2\u4e4b\u4e0d\u540c\uff0c\u5206\u5225\u91dd\u5c0d\u96d9\u80a1\uff08double strand\uff09\u8207\u55ae\u80a1\uff08single strand\uff09DNA\u65b7\u88c2\u9032\u884c\u5075\u6e2c\u3002<\/li>\n
    2. \u7de9\u885d\u6db2\u53ef\u4f7f\u7528\u4e2d\u6027\uff08Neutral\uff09\u6216\u9e7c\u6027\uff08Alkaline\uff09\u5169\u7a2e\u4e0d\u540c\u7684\u7de9\u885d\u6db2\uff0c\u9032\u884c\u591a\u7a2eDNA\u65b7\u88c2\u5206\u6790\u3002<\/li>\n
    3. \u9664\u61c9\u7528\u5728DNA damage\u3001\u7d30\u80de\u51cb\u4ea1\u5916\uff0c\u4ea6\u53ef\u5206\u6790\u85e5\u7406\u6bd2\u6027\u3001Sperm DNA fragmentation\uff08in vitro fertilization\uff09\u3002<\/li>\n<\/ol>\n<\/ol>\n

      \u64cd\u4f5c\u6d41\u7a0b\uff1a<\/strong><\/span><\/p>\n

      \"comet_procedure\"<\/p>\n

        \n
          \n
        1. \u5f85\u6e2c\u7d30\u80de\u8207\u4f4e\u7194\u9ede\u74ca\u8102\u81a0 \uff08LM Agarose\uff09\uff0c\u572837o<\/sup>C\u4e0b\u5747\u52fb\u6df7\u5408\u3002<\/li>\n
        2. \u5c07\u7d30\u80de\u653e\u7f6e\u5728CometSlide\u2122\u4e0a\u3002<\/li>\n
        3. \u7d66\u4e88\u7d30\u80deLysis Solution\uff0c\u79fb\u9664\u7d30\u80de\u819c\u8207DNA\u4e0a\u7684histones\u3002<\/li>\n
        4. \u52a0\u5165\u8a66\u5291\u4f7fDNA\u8b8a\u6027\u3001\u89e3\u65cb\u3002<\/li>\n
        5. \u6a23\u54c1\u4f7f\u7528\u6838\u9178\u67d3\u5291\u9032\u884c\u67d3\u8272\uff0c\u4e26\u4f7f\u7528\u87a2\u5149\u986f\u5fae\u93e1\uff0c\u89c0\u6e2c\u96fb\u6cf3\u5f8c\u65b7\u88c2DNA\u79fb\u52d5\u7684\u60c5\u5f62\u3002\u00a0<\/span><\/li>\n<\/ol>\n<\/ol>\n

          \"Comet_electrophoresis_unit\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
          \n

          \u5546\u54c1\u578b\u865f<\/strong><\/p>\n<\/td>\n

          		\n

          \u7522\u54c1\u540d\u7a31<\/strong><\/p>\n<\/td>\n

          		\n

          \u5305\u88dd\u898f\u683c<\/strong><\/p>\n<\/td>\n<\/tr>\n

          \n

          Comet Assay\u5206\u6790\u8a66\u5291\u7d44<\/strong><\/p>\n<\/td>\n<\/tr>\n

          \n

          4250-050-K<\/p>\n<\/td>\n

          		CometAssay\u00ae Kit\uff0825X2 well slides\uff09<\/td>\n\n

          50 samples<\/p>\n<\/td>\n<\/tr>\n

          \n

          4252-040-K<\/p>\n<\/td>\n

          		CometAssay\u00ae Kit\uff082X20 well slides\uff09<\/td>\n\n

          40 samples<\/p>\n<\/td>\n<\/tr>\n

          \n

          4253-096-K<\/p>\n<\/td>\n

          		CometAssay\u00ae Kit\uff081X96 well slide\uff09<\/td>\n\n

          96 samples<\/p>\n<\/td>\n<\/tr>\n

          \n

          Comet Assay\u5c08\u7528\u73bb\u7247<\/strong><\/p>\n<\/td>\n<\/tr>\n

          \n

          4250-050-03
          \n4250-200-03<\/p>\n<\/td>\n

          		CometSlides\u2122 \u5c08\u7528\u73bb\u7247\uff082 Well\uff09<\/td>\n\n

          25 \u7247
          \n100 \u7247<\/p>\n<\/td>\n<\/tr>\n

          \n

          4252-040-01
          \n4252-200-01
          \n4252-500-01
          \n4252-02K-01<\/p>\n<\/td>\n

          		CometAssay\u00ae HT \u5c08\u7528\u73bb\u7247\uff0820 Well\uff09<\/td>\n\n

          2 \u7247
          \n10 \u7247
          \n25 \u7247
          \n100 \u7247<\/p>\n<\/td>\n<\/tr>\n

          \n

          4253-096-03<\/p>\n<\/td>\n

          		CometSlide\u2122 \u5c08\u7528\u73bb\u7247\uff0896 Well\uff09<\/td>\n\n

          1 \u7247<\/p>\n<\/td>\n<\/tr>\n

          \n

          Comet Assay \u5c08\u7528\u8a66\u5291<\/strong><\/p>\n<\/td>\n<\/tr>\n

          \n

          4250-010-01<\/p>\n<\/td>\n

          		CometAssay Lysis Solution<\/td>\n\n

          100 ml<\/p>\n<\/td>\n<\/tr>\n

          \n

          4250-050-02<\/p>\n<\/td>\n

          		CometAssay\u00ae LM Agarose<\/td>\n\n

          15 ml<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

          \u66f4\u591a\u76f8\u95dc\u5546\u54c1<\/span> \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a> | Agarose | \u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> | CMOS\u87a2\u5149\u76f8\u6a5f<\/a> |Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

          \u3000\u3000\u7f8e\u570bTREVIGEN\u516c\u53f8\u6210\u7acb\u65bc1992\u5e74\u3002\u81f4\u529b\u65bc\u7814\u767c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u3001DNA\u65b7\u640d\u8207\u4fee\u5fa9\u3001\u9ad4\u5167\u8840\u7ba1\u65b0\u751f\u5206\u6790\u5957\u7d44\uff08 […]<\/p>\n","protected":false},"author":3,"featured_media":4256,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[515,183],"tags":[4776],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/657"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=657"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/657\/revisions"}],"predecessor-version":[{"id":6413,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/657\/revisions\/6413"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4256"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=657"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=657"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=657"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}