{"id":6560,"date":"2016-09-13T13:30:48","date_gmt":"2016-09-13T05:30:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6560"},"modified":"2022-07-26T11:45:37","modified_gmt":"2022-07-26T03:45:37","slug":"%ce%b2-actin%e8%82%8c%e5%8b%95%e8%9b%8b%e7%99%bd%e6%8a%97%e9%ab%94-loading-control%e5%a4%9a%e6%a0%aa%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f3598r-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6560","title":{"rendered":"GAPDH\u6297\u9ad4 | loading contro\u591a\u682a\u6297\u9ad4 \u8ca8\u865f3777R-100"},"content":{"rendered":"

Biovision \u63d0\u4f9b\u4e00\u7cfb\u5217\uff0cloading control\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\uff1b\u91dd\u5c0d GAPDH\u3001\u03b1-tubulin\u3001\u03b1-actin\u3001\u03b2-actin \u7b49\u55ae\/\u591a\u682a\u5167\u63a7\u6297\u9ad4\uff0c\u53ef\u505a\u70baStandardize\/Normalize\u7684\u4f9d\u64da\u3002<\/p>\n

Beta-Actin\u55ae\u682a\u6297\u9ad4<\/a> | GAPDH\u591a\u682a\u6297\u9ad4<\/a> | Tubulin\u591a\u682a\u6297\u9ad4<\/a> | VDAC\/Porin\u591a\u682a\u6297\u9ad4<\/a> | Histone H3\u591a\u682a\u6297\u9ad4<\/a><\/p>\n

GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) <\/strong>loading control<\/strong>\u591a\u682a\u6297\u9ad4<\/strong> | GAPDH Antibody | 3777R-100<\/strong><\/p>\n

\u7d30\u80de\u8cea\u6a19\u8a8c ( Cytoplasmic marker ) \u5728\u5927\u90e8\u5206\u7d30\u80de\u53ca\u7d44\u7e54\u4e2d\u7a69\u5b9a\u4e14\u6301\u7e8c\u9ad8\u5ea6\u8868\u73fe.\u514d\u75ab\u539f\u70ba\u5408\u6210\u4e4b GADPH (\u80fa\u57fa317)\u80dc\u80bd\u3001\u53cd\u61c9\u7269\u7a2ehuman, mouse, rat\uff0c\u53ef\u61c9\u7528\u65bcWestern blot\u8a66\u9a57\u3002<\/p>\n

\"GAPDH<\/p>\n

\u7269\u7a2e\u4f86\u6e90: Rabbit \u591a\u682a\u6297\u9ad4Polyclonal \u540c\u578b\u5c0d\u7167\u7d44Isotype: Rabbit IgG<\/p>\n

\u514d\u75ab\u4f86\u6e90 Synthetic peptide surrounding amino acid 317of rat GADPH<\/p>\n

\u7269\u7a2e\u53cd\u61c9: human, mouse, rat<\/p>\n

\u61c9\u7528 Western blot, Western blotting (0.5-4 \u00b5g\/ml). However, the optimal concentrations should be determined individually. The antibody recognizes over 100 kDa band corresponding to NIF-1 in samples from human origin. Reactivity to other species has not been tested.<\/p>\n

\"\u672a\u547d\u540d-14\"\u00a0\u03b2-actin<\/strong>\u808c\u52d5\u86cb\u767d\u6297\u9ad4<\/strong> | <\/strong>\u7d30\u80de\u8cea\u6a19\u8a8c ( Cytoplasmic marker ) <\/strong>loading control<\/strong>\u591a\u682a<\/strong>\u6297\u9ad4<\/strong> |<\/strong> \u8ca8\u865f<\/strong>3598R-100\u00a0<\/strong><\/p>\n

\u7d30\u80de\u8cea\u6a19\u8a8c ( Cytoplasmic marker ). \u03b2-Actin\u662f\u4e00\u7a2e\u5b58\u5728\u6240\u6709\u771f\u6838\u7d30\u80de ( \u7dda\u87f2\u7cbe\u5b50\u4f8b\u5916 ) \u4e2d\u7403\u5f62\u86cb\u767d\u8cea\uff0c\u6700\u9ad8\u5ea6\u4fdd\u7559 ( conserved ) \u86cb\u767d\u4e4b\u4e00. \u808c\u52d5\u86cb\u767d\u662f\u5728\u6240\u6709\u771f\u6838\u7d30\u80de\u4e2d\u8868\u9054\uff0c\u4e26\u4e14\u662f\u7d30\u80de\u9aa8\u67b6\u7684\u4e3b\u8981\u6210\u5206\u3002\u81f3\u5c11\u516d\u7a2e\u985e\u578b\u7684\u808c\u52d5\u86cb\u767d\u7684\u5b58\u5728\u65bc\u54fa\u4e73\u52d5\u7269\u7d44\u7e54\u4e2d\uff0c\u4e26\u5206\u6210\u4e09\u985e\u3002\u963f\u723e\u6cd5\u52d5\u86cb\u767dAlpha actin\u7684\u8868\u9054\u88ab\u9650\u5236\u5728\u5404\u7a2e\u985e\u578b\u7684\u808c\u8089\u548c\u5b83\u8abf\u7bc0contactile\u96fb\u4f4d\u70ba\u808c\u7d30\u80de\uff0c\u800c\u03b2\u548c\u03b3\u808c\u52d5\u86cb\u767d\uff0c\u4e5f\u88ab\u7a31\u70ba\u7d30\u80de\u8cea\u808c\u52d5\u86cb\u767d\uff0c\u5728\u975e\u808c\u8089\u7d30\u80de\u4e2d\u8868\u9054\uff0c\u63a7\u5236\u7d30\u80de\u7d50\u69cb\u548c\u904b\u52d5\u6027\u3002<\/p>\n

\"Beta-Actin<\/p>\n

\u7269\u7a2e\u4f86\u6e90: mouse \u55ae\u682a\u6297\u9ad4monoclonal \u540c\u578b\u5c0d\u7167\u7d44Isotype: Mouse IgG2b\u00a0\u514d\u75ab\u4f86\u6e90\u03b2-Actin N-terminal peptide-KLH conjugates.<\/p>\n

\u7269\u7a2e\u53cd\u61c9: Species Reactivity: Human, mouse, rat, rabbit, and chicken\u00a0Application: Western blot\u61c9\u7528 Western blot: ~1:1000 \u2013 1:5000. 1 mg\/ml\u00a0Concentration: 1 mg\/ml\u00a0<\/strong><\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Tubulin<\/strong>\u591a\u682a\u6297\u9ad4<\/strong> | Tubulin Antibody<\/strong> loading control<\/strong> | 3708-100<\/strong>\u898f\u683c<\/strong>100ug<\/strong><\/p>\n

Tubulin\u662f\u4e00\u7a2e\u7d30\u80de\u8cea\u6a19\u8a8c ( Cytoplasmic marker )\u86cb\u767d\uff0c\u70ba\u7d50\u69cb\u86cb\u767d\u5fae\u7ba1 ( Microtubules )\u7684\u6210\u5206. \u9ad8\u5ea6\u4fdd\u7559 ( highly conserved ) \u7684\u86cb\u767d\u8cea\u3002\u5fae\u7ba1( Microtubules )\u662f\u4e00\u7a2e\u7d30\u80de\u9aa8\u67b6\u3002\u7403\u72c0\u5fae\u7ba1\u86cb\u767d\u4e9e\u55ae\u4f4d\u662f\u5fae\u7ba1\u548c\u03b1\u548c\u03b2\u5fae\u7ba1\u86cb\u767d\u7570\u6e90\u4e8c\u805a\u9ad4\u5f62\u5f0f\u7684\u5fae\u7ba1\u86cb\u767d\u4e9e\u55ae\u4f4d\uff0c\u5b58\u5728\u65bc\u6240\u6709\u771f\u6838\u7d30\u80de\u4e2d\uff0c\u03b1\u3001\u03b2 & \u03b3 tubulins \u5e38\u88ab\u4f7f\u7528\u70ba loading control\uff0c\u53c3\u8207\u8a31\u591a\u7d30\u80de\u7684\u904b\u52d5\u3002<\/p>\n

\"Tubulin<\/p>\n

\u7269\u7a2e\u4f86\u6e90: Rabbit \u591a\u682a\u6297\u9ad4Polyclonal \u540c\u578b\u5c0d\u7167\u7d44Isotype: Rabbit IgG<\/p>\n

\u514d\u75ab\u4f86\u6e90 Synthetic peptide corresponding to the C-terminus of human \u03b2-Tubulin<\/p>\n

\u7269\u7a2e\u53cd\u61c9: human, mouse, rat<\/p>\n

\u61c9\u7528 Western blot, Western blotting (0.5-4 \u00b5g\/ml). However, the optimal concentrations should be determined individually. HeLa cell or Jurkat cell lysate can be used as a positive control. The antibody recognizes 55 kDa \u03b2-tubulin in samples of human, mouse, and rat origins. Reactivity to other species has not been determined.\u00a0Concentration: 0.5 mg\/ml<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0VDAC\/Porin<\/strong>\u591a\u682a\u6297\u9ad4<\/strong> |<\/strong> VDAC\/Porin Antibody<\/strong> loading control<\/strong> | 3594-100<\/strong>\u898f\u683c<\/strong>100ug<\/strong><\/p>\n

\u7c92\u7dda\u9ad4\u6a19\u8a8c ( Mitochondrial marker )\u3002\u96fb\u58d3\u4f9d\u8cf4\u6027\u9670\u96e2\u5b50\u901a\u9053\uff08VDAC\u6216\u7dda\u7c92\u9ad4\u5b54\u86cb\u767d\uff09I\u662f\u5916\u819c\u7dda\u7c92\u9ad4\u86cb\u767d\u3002VDAC\u86cb\u767d\u662f\u4ee5\u901a\u904e\u8a72\u817a\u560c\u5464\u6838\u82f7\u9178\u901a\u904e\u7dda\u7c92\u9ad4\u5916\u819c\u4e3b\u8981\u7684\u5b54\u3002Immunogen: Synthetic peptide surrounding amino acid 189 of human VDAC<\/p>\n

\"VDAC\/Porin<\/p>\n

\u7269\u7a2e\u4f86\u6e90: Rabbit \u591a\u682a\u6297\u9ad4Polyclonal \u540c\u578b\u5c0d\u7167\u7d44Isotype: Rabbit IgG<\/p>\n

\u514d\u75ab\u4f86\u6e90 Synthetic peptide surrounding amino acid 189 of human VDAC<\/p>\n

\u7269\u7a2e\u53cd\u61c9: human, mouse, rat, bovine, rabbit<\/p>\n

\u61c9\u7528 Western blot, Immunohistochemistry, Western blotting (0.5-4 \u00b5g\/ml) and Immunohistochemistry (2.5 \u00b5g\/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~31 kDa VDAC\/Porin from samples of human, mouse, rat, bovine, pig, and rabbit origins. Concentration: 0.5 mg\/ml<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Histone H3 Antibody<\/strong>\u591a\u682a\u6297\u9ad4<\/strong> |<\/strong> Histone H3 Antibody<\/strong> loading control<\/strong> | 3623-100<\/strong>\u898f\u683c<\/strong>100ug<\/strong><\/p>\n

\u6297\u9ad4\u4ecb\u7d39:\u00a0\u6838\u5c0f\u9ad4\u662f\u7531\u56db\u500b\u6838\u5fc3\u7d44\u86cb\u767d\uff08H2A\uff0cH2B\uff0cH3\u548cH4\uff09\u7684\u548c\u662f\u67d3\u8272\u8cea\u7684\u4e3b\u8981\u7d44\u6210\u90e8\u5206\u3002\u6838\u5fc3\u7d44\u86cb\u767d\u7684N\u672b\u7aef\u5c3e\u90e8\u5728\u7d30\u80de\u63a5\u53d7\u8a0a\u606f\u523a\u6fc0\u5f8c\uff0c\u7d93\u7531\u8f49\u9304\u5f8c\u4fee\u98fe\uff0c\u5305\u62ec\u4e59\u9170\u5316\uff0c\u78f7\u9178\u5316\u548c\u7532\u57fa\u5316\u3002\u4e26\u76f4\u63a5\u5f71\u97ff\u57fa\u56e0\u8868\u9054\u3002<\/p>\n

\u7269\u7a2e\u4f86\u6e90: Rabbit \u591a\u682a\u6297\u9ad4Polyclonal \u540c\u578b\u5c0d\u7167\u7d44Isotype: Rabbit IgG<\/p>\n

\u514d\u75ab\u4f86\u6e90 Synthetic peptide near the C-terminus of human Histone H3<\/p>\n

\u7269\u7a2e\u53cd\u61c9: human, mouse, rat<\/p>\n

\u61c9\u7528 Western blot, Immunohistochemistry Application & Usage: Western blotting (0.5-4 \u00b5g\/ml)and Immunohistochemistry (20 \u00b5g\/ml). However, the optimal conditions should be determined individually. The antibody detects 17-20 kDa histone H3 protein. It does not cross-react with other histones.<\/p>\n

\"Histone<\/p>\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0loading control\u55ae\u591a\u682a\u6297\u9ad4<\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a03662-100<\/td>\n\u00a0Beta-Actin Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a03850-100<\/td>\n\u00a0Beta-Actin Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a03638-100<\/td>\n\u00a0Cox-4 Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a03777-100<\/td>\n\u00a0GAPDH Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5l<\/td>\n<\/tr>\n
\u00a03623-100<\/td>\n\u00a0Histone H3 Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a03708-100<\/td>\n\u00a0Tubulin Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a03594-100<\/td>\n\u00a0VDAC\/Porin Antibody<\/a><\/strong><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Primary<\/a>\u00a0and\u00a0Secondary Antibodies<\/a>\u4e00\u7d1a\u6297\u9ad4\u8207\u4e8c\u7d1a\u6297\u9ad4\u00a0| Phospho-specific Antibodies<\/a>\u78f7\u9178\u5316\u6297\u9ad4 | Tag\/<\/a>Marker<\/a>\/Loading Control Antibodies<\/a>\u00a0| IHC\/ChIP\/WB\/ELISA Tested Antibodies<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Biovision \u63d0\u4f9b\u4e00\u7cfb\u5217\uff0cloading control\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\uff1b\u91dd\u5c0d GAPDH\u3001\u03b1-tubuli […]<\/p>\n","protected":false},"author":3,"featured_media":6494,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[90,476,3],"tags":[4485],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6560"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6560"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6560\/revisions"}],"predecessor-version":[{"id":8585,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6560\/revisions\/8585"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6494"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6560"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6560"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6560"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}