{"id":6469,"date":"2016-09-08T23:00:57","date_gmt":"2016-09-08T15:00:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6469"},"modified":"2025-01-20T16:10:09","modified_gmt":"2025-01-20T08:10:09","slug":"2xyt-agar-m13%e5%99%ac%e8%8f%8c%e9%ab%94%e7%94%9f%e9%95%b7%e4%b9%8b%e5%9f%b9%e9%a4%8a%e7%93%8a%e8%84%82%e5%87%9d%e8%86%a0-%e8%b2%a8%e8%99%9flab180","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6469","title":{"rendered":"2xYT Agar M13\u566c\u83cc\u9ad4\u751f\u9577\u4e4b\u57f9\u990a\u74ca\u8102\u51dd\u81a0 |\u8ca8\u865fLAB180"},"content":{"rendered":"

2xYT Agar\u00a0M13\u566c\u83cc\u9ad4\u751f\u9577\u4e4b\u57f9\u990a\u74ca\u8102\u51dd\u81a0 |<\/strong>\u8ca8\u865f<\/strong>LAB180<\/strong><\/a><\/span><\/p>\n

2\u00d7YT\u57f9\u990a\u74ca\u8102\u51dd\u81a0\uff0c\u63d0\u4f9b\u7d72\u72c0\u55ae\u93c8\u566c\u83cc\u9ad4\u5bbf\u4e3b\u7d30\u80de\u7684\u751f\u9577\uff0c\u4f8b\u5982M13\u566c\u83cc\u9ad4\u3002An agar version of 2xYT broth, for the growth of host cells of filamentous single stranded bacteriophages e.g. the M13 phage.<\/p>\n

\"2xYT<\/a><\/p>\n

2xYT Medium<\/strong>\u00a0\u00a0<\/a>(LAB179)<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Tryptone Soy Broth TSB: <\/strong>\u7d30\u83cc\u548c\u771f\u83cc\u751f\u9577\u4e4b\u57f9\u990a\u57fa | \u8ca8\u865fLAB004-A<\/strong><\/p>\n

\u9069\u7528\u65bc\u7d30\u83cc\u548c\u771f\u83cc\u7684\u4e00\u7a2e\u901a\u7528\u578b\u7684\u71df\u990a\u57f9\u990a\u57fa\u3002\u70baHarmonised\u85e5\u5178\uff08USP\/ EP\/ JP\uff09\u4e2d\uff0c\u85e5\u7269\u7522\u54c1\u7684\u7121\u83cc\u6e2c\u8a66\u8981\u6c42\u4e4b\u57f9\u990a\u57fa\u3002\u4ea6\u5ee3\u6cdb\u7528\u65bc\u8840\u57f9\u990a\u57fa\uff0c\u65bc\u9ad8\u6c34\u5e73\u4e4b\u78b3\u6c34\u5316\u5408\u7269\u4e0b\u53ef\u8fc5\u901f\u589e\u9577\u548c\u96a8\u5f8c\u9178\u751f\u7522\u5f8c\u96a8\u5f8c\u6b7b\u4ea1\u3002The medium is widely used for blood cultures although the high carbohydrate level may cause rapid growth and subsequent death of acid-producing organisms<\/p>\n

\"Tryptone<\/a>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 \"Tryptone<\/a><\/p>\n

Tryptone Soy Agar (TSA)<\/strong>\u00a0\u00a0 <\/a>\u00a0 Tryptone Soy Broth without dextrose (TSB)<\/strong> <\/a><\/p>\n

Plate count agar<\/strong>\uff0c<\/strong>PCA<\/strong>\u57f9\u990a\u76bf\u8a08\u6578\u57f9\u990a\u57fa<\/strong>\\<\/strong>\u7e3d\u751f\u83cc\u6578\u57f9\u990a\u57fa<\/strong> | <\/strong>\u8ca8\u865f<\/strong>LAB149-A<\/strong><\/p>\n

\u570b\u969b\u6a19\u6e96PCA\uff0c\u7528\u65bc\u7e3d\u7d30\u83cc\u4e2d\u7684\u6e2c\u5b9a\uff08GB2008\u3001SN\u6a19\u6e96\uff09\u3002\u5546\u54c1\u5316\u7d30\u83cc\u7528\u57f9\u990a\u57faPCA\uff1a\u4e3b\u8981\u7528\u65bc\u7d30\u83cc\u57f9\u990a\uff1b\u6216\u4ea6\u53ef\u9078\u53d6\u80f0\u86cb\u767d\u5927\u8c46\u74ca\u8102\u57f9\u990a\u57fa\uff08Tryptone soy agar\uff0cTSA\uff09\u4e8c\u8005\u4e4b\u4e00\u57f9\u990a\u7d30\u83cc\u3002<\/p>\n

LAB M\u00a0 <\/strong>\u7c89\u672b\u57f9\u990a\u57fa | \u82f1\u570b\u539f\u88dd\u9032\u53e3<\/strong><\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Harlequin\u2122<\/span>\u5448\u8272\u57f9\u990a\u57fa\u7cfb\u5217\u5546\u54c1<\/p>\n

LAB M\u4f4d\u65bc\u82f1\u570b\uff0c\u662f\u57f9\u990a\u57fa\u8001\u54c1\u724c\u516c\u53f8\uff0c\u53e3\u7891\u597d\u54c1\u8cea\u4f73\uff0c\u63a1\u7528\u5bc6\u5c01\u5c01\u53e3\u819c\uff0c\u539f\u88dd\u9032\u53e3\u57f9\u990a\u57fa\u4e0d\u6613\u53d7\u6f6e\uff0c\u4ea6\u6c92\u6709\u5206\u88dd\u9810\u6df7\u4e4b\u7591\u616e\u3002Harlequin\u2122<\/span>\u5448\u8272\u57f9\u990a\u57fa\u7cfb\u5217\u5546\u54c1\u8207\u5404\u5f0f\u57f9\u990a\u57fa\u6dfb\u52a0\u7269\uff0c\u63d0\u4f9b\u5feb\u901f\u548c\u6e96\u78ba\u7684\u65b9\u6cd5\u5206\u96e2\u8207\u5224\u8b80\u76ee\u6a19\u5fae\u751f\u7269\u3002\u96d9\u8272\u5224\u8b80\uff0c\u7c21\u6613\u5feb\u901f\uff1b\u5728\u98df\u54c1\u5b89\u5168\u53ca\u81e8\u5e8a\u8a3a\u65b7\u7686\u7b26\u5408\u76f8\u95dc\u898f\u7bc4\uff08ISO 9001:2000\u548cISO13485\uff0c\u4ee5\u53ca\u6b50\u6d32IVD\u6307\u4ee4CE\u6a19\u8a8c\uff09\u3002\"New\u00a0:\u00a0CXCR4<\/p>\n

\"HAL008\"\"PIN001-web\"\"PIN005-web\"<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u5206\u751f\u5c08\u7528\u57f9\u990a\u57faLB Broth\u8207LB Agar\uff0c\u65b9\u4fbf\u9032\u884cDNA Cloning\u3001Yeast\u57f9\u990a\u7b49\u5be6\u9a57\uff0c\u662f E. Coli \u8868\u73fe\u7cfb\u7d71\u6700\u4f73\u7684\u9078\u64c7\uff01<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Tryptone <\/b>\u916a\u86cb\u767d<\/strong>(casein)<\/strong>\u6c34\u89e3\u7269<\/strong> | <\/strong>\u8ca8\u865f<\/strong>MC005-A<\/strong><\/p>\n

Tryptone\u5e38\u898b\u65bc\u5fae\u751f\u7269\u57f9\u990a\u8207\u767c\u9175\u4f7f\u7528\uff0c\u4e5f\u662f\u7f8e\u570b\u85e5\u5178\u898f\u7bc4\u7684\u6a19\u6e96\u57f9\u990a\u57fa\u6210\u5206\u4e4b\u3127\u3002Tryptone\u662f\u916a\u86cb\u767d(casein)\u7d93\u7531\u80f0\u81df\u9175\u7d20\u6c34\u89e3(pancreatic digest)\u800c\u6210\u7684\u71df\u990a\u6210\u5206\uff0c\u4e5f\u662f\u4e00\u822c\u6240\u8b02\u80f0\u6c34\u89e3\u86cb\u767d\u7684\u901a\u7528\u540d\u7a31\u3002\u916a\u86cb\u767d\u662f\u725b\u5976\u4e2d\u4e3b\u8981\u7684\u86cb\u767d\u8cea\u6210\u5206\uff0c\u5bcc\u542b\u5404\u7a2e\u80fa\u57fa\u9178\u6c2e\u6e90\uff0c\u5c24\u5176\u662f\u8272\u80fa\u9178(tryptophan)\u6bd4\u4f8b\u76f8\u7576\u9ad8\u3002Tryptone\u7684\u7528\u9014\u5f88\u5ee3\uff0c\u662fLB medium\u3001Terrific broth\u3001Tryptic soy medium (TSB)\u8207Fluid Thioglycollate Medium (FTM)\u7b49\u5e38\u7528\u57f9\u990a\u57fa\u7684\u4e3b\u8981\u6210\u5206\uff0c\u4e5f\u662f\u6297\u751f\u7d20\u3001\u80de\u5916\u86cb\u767d\u8cea(extra-cellular protein)\u3001\u5e72\u64fe\u7d20(interferon)\u8207\u75ab\u82d7\u6bd2\u7d20\u7b49\u767c\u9175\u88fd\u7a0b\u4e2d\u91cd\u8981\u7684\u57f9\u990a\u539f\u6599\u3002<\/p>\n

\"\"<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a> Yeast extract | <\/strong>\u9175\u6bcd\u8403\u53d6\u7269<\/strong>(yeast extract) |<\/strong>\u8ca8\u865f<\/strong>MC001-A<\/strong><\/p>\n

\u53ef\u4ee5\u63d0\u9ad8 E. Coli DH5\u03b1 \u5bc6\u5ea6\u9054\u50b3\u7d71 LB broth \u3002Yeast extract \u63d0\u4f9b\u4e86\u8c50\u5bcc\u7684\u71df\u990a\u6210\u5206\uff0cSaccharomyces\u9175\u6bcd\u7d30\u80de\u7d93\u904e\u81ea\u52d5\u5206\u89e3(autolysate)\u5f8c\uff0c\u9664\u4e86\u5bcc\u542b\u5404\u5f0f\u5fae\u751f\u7269\u4ee5\u53ca\u52d5\u7269\u7d30\u80de\u751f\u9577\u6240\u9700\u7684\u80fa\u57fa\u9178\u6c2e\u6e90\u5916\uff0c\u9084\u6709\u542b\u91cf\u6975\u9ad8\u7684\u53ef\u6eb6\u6027\u7dad\u751f\u7d20B\u7fa4\u3002\u5305\u62ec vitamins\u3001amino acids\u3001peptides \u8207 carbohydrates \u7b49\u3002\u5176\u9ad8\u71df\u990a\u50f9\u503c\u4e5f\u662f\u80ce\u725b\u8840\u6e05 FBS \u6700\u4f73\u66ff\u4ee3\u7269\uff0c\u662f\u751f\u7269\u88fd\u7a0b\u4e2d\u6975\u5177\u91cd\u8981\u6027\u7684\u975e\u52d5\u7269\u6027\u86cb\u767d\u4e4b\u4f86\u6e90\u3002\u56e0\u6b64\u57f9\u990a\u57fa\u914d\u65b9\u3001\u5fae\u751f\u7269\u767c\u9175\u539f\u6599\u3001\u8840\u6e05\u66ff\u4ee3\u7269\u3001\u6297\u9ad4\u751f\u7522\u8207\u86cb\u767d\u8cea\u85e5\u7269\u7b49\u90fd\u63a1\u7528yeast extract\u4f5c\u70ba\u71df\u990a\u4f86\u6e90\u3002<\/p>\n

\"Yeast<\/p>\n

\u9ef4\u83cc\u53ca\u9175\u6bcd\u83cc\u57f9\u990a\uff0c\u4f7f\u7528\u6c99\u6c0f\u8461\u8404\u7cd6\u57f9\u990a\u57fa\uff08Sabouraud Dextrose Agar\uff0cSDA\uff09 \u6216\u99ac\u9234\u85af\u8461\u8404\u7cd6\u57f9\u990a\u57fa\uff08Potato dextrose agar\uff0cPDA\uff09\u3002Potato Dextrose Agar \u00a0| \u99ac\u9234\u85af\u8461\u8404\u7cd6\u6d0b\u83dc\u57f9\u990a\u57fa |\u8ca8\u865fLAB098-A<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
LAB004-A<\/td>\nTryptone Soy Broth USP (Soybean Casein Digest Medium)<\/td>\n500g<\/td>\n<\/tr>\n
LAB011-A<\/td>\nTryptone Soy Agar USP (Soybean Casein Digest Medium)<\/td>\n500g<\/td>\n<\/tr>\n
LAB028-A<\/td>\nBlood Agar Base<\/td>\n500g<\/td>\n<\/tr>\n
LAB061-A<\/td>\nEosin Methylene Blue Agar (Levine)<\/td>\n500g<\/td>\n<\/tr>\n
LAB093-A<\/td>\nMRS Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB094-A<\/td>\nMRS Broth<\/td>\n500g<\/td>\n<\/tr>\n
LAB098-A<\/td>\nPotato Dextrose Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB108-A<\/td>\nPseudomonas Agar Base<\/td>\n500g<\/td>\n<\/tr>\n
LAB149-A<\/td>\nPlate Count Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB168-A<\/td>\nL.B. Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB169-A<\/td>\nLB Broth<\/td>\n500g<\/td>\n<\/tr>\n
LAB196-A<\/td>\nLauryl Tryptose Broth<\/td>\n500g<\/td>\n<\/tr>\n
MC001-A<\/td>\nYeast Extract Powder<\/td>\n500g<\/td>\n<\/tr>\n
MC003-A<\/td>\nSoy Peptone<\/td>\n500g<\/td>\n<\/tr>\n
MC005-A<\/td>\nTryptone<\/td>\n500g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u66f4\u591a\u76f8\u95dc\u5546\u54c1<\/span> \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| Agarose |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> |Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>| NEOGEN\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

2xYT Agar\u00a0M13\u566c\u83cc\u9ad4\u751f\u9577\u4e4b\u57f9\u990a\u74ca\u8102\u51dd\u81a0 |\u8ca8\u865fLAB180 2\u00d7YT\u57f9\u990a\u74ca\u8102\u51dd\u81a0\uff0c\u63d0\u4f9b\u7d72\u72c0\u55ae\u93c8\u566c\u83cc […]<\/p>\n","protected":false},"author":3,"featured_media":6470,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[475,88],"tags":[4506],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6469"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6469"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6469\/revisions"}],"predecessor-version":[{"id":50587,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6469\/revisions\/50587"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6470"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6469"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6469"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6469"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}