{"id":6466,"date":"2016-09-08T16:42:09","date_gmt":"2016-09-08T08:42:09","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6466"},"modified":"2022-07-26T13:58:13","modified_gmt":"2022-07-26T05:58:13","slug":"cd55-crispr%e5%9f%ba%e5%9b%a0%e5%89%94%e9%99%a4%e7%b4%b0%e8%83%9e-mcf7-%e8%b2%a8%e8%99%9fk0403257","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6466","title":{"rendered":"CD55 CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de (MCF7) |\u8ca8\u865fK0403257"},"content":{"rendered":"<p><strong>CD5<\/strong><strong>5 <\/strong><strong>CRISP<\/strong><strong>R<\/strong><strong>\u57fa\u56e0\u5254\u9664\u7d30\u80de<\/strong><strong> (<\/strong><strong>MCF7)<\/strong><strong> |<\/strong><strong>\u8ca8\u865f<\/strong><strong>K0403257| <\/strong><strong>\u5305\u88dd<\/strong><strong>1&#215;10<sup>6<\/sup> cells \/ 1.0 ml<\/strong><\/p>\n<p>CD55 CRISPR Knock Out MCF7 Cell Line (Human)This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.<\/p>\n<p>\u4eba\u985eMCF7 CD55 knock out\u7d30\u80de\u682a | Puromycin (0.5\u03bcg\/ml) | \u7d30\u80de\u57f9\u990a\u689d\u4ef6DMEM, 10%FBS and 1%P\/S, 37\u00b0C, 5% CO2 | <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/nuccore\/NM_001114752\">NM_001114752<\/a> <a href=\"https:\/\/www.abmgood.com\/CD55-CRISPR-K0403257.html\">pLenti-U6-sgRNA-PGK-Neo<\/a><\/p>\n<table width=\"88%\">\n<tbody>\n<tr>\n<td width=\"67%\"><strong>Add-On Services<sup>^<\/sup><\/strong><\/td>\n<td width=\"18%\"><strong>Quantity<\/strong><\/td>\n<td width=\"13%\"><strong>Cat. No.<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Cell Line of Your Choice for Gene Editing<sup>#<\/sup><\/td>\n<td>1 Cell Line<\/td>\n<td>C141<\/td>\n<\/tr>\n<tr>\n<td>WT Control Cell Line Expressing Cas9 for Comparison<\/td>\n<td>1 Cell Line<\/td>\n<td>C142<\/td>\n<\/tr>\n<tr>\n<td>Additional Clones<\/td>\n<td>1 Clone<\/td>\n<td>C143<\/td>\n<\/tr>\n<tr>\n<td>Additional Vials of Delivered Clones<\/td>\n<td>1 Vial<\/td>\n<td>C144<\/td>\n<\/tr>\n<tr>\n<td>Validation Service by Western Blot (Up to 10 Clones)<sup>##<\/sup><\/td>\n<td>1 Service<\/td>\n<td>C145<\/td>\n<\/tr>\n<tr>\n<td>Off-Target Analysis by Whole Genome Sequencing<\/td>\n<td>Per Clone<\/td>\n<td>C146<\/td>\n<\/tr>\n<tr>\n<td>Additional rounds of selection and screening by Sanger Sequencing<\/td>\n<td>1 Screening<\/td>\n<td>C147<\/td>\n<\/tr>\n<tr>\n<td>STR Profiling of WT and Knock-out<\/td>\n<td>1 Service<\/td>\n<td>C287<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?cat=443\">\u7d30\u80de\u6c61\u67d3\u9632\u6cbb\u5546\u54c1<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?cat=57\">\u6162\u75c5\u6bd2\u611f\u67d3\u7cfb\u7d71<\/a> |\u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=5370\">G418\u7be9\u9078\u578b\u6297\u751f\u7d20<\/a> |<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CD55 CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de (MCF7) |\u8ca8\u865fK0403257| \u5305\u88dd1&#215;106 cell [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":6459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[4507],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6466"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6466"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6466\/revisions"}],"predecessor-version":[{"id":6467,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6466\/revisions\/6467"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6466"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6466"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6466"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}