{"id":6414,"date":"2016-09-06T13:52:33","date_gmt":"2016-09-06T05:52:33","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6414"},"modified":"2022-07-26T14:02:44","modified_gmt":"2022-07-26T06:02:44","slug":"dnase-i-%e5%8e%bb%e6%b0%a7%e6%a0%b8%e7%b3%96%e6%a0%b8%e9%85%b8%e9%85%b6i-deoxyribonuclease-i-2000-u","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6414","title":{"rendered":"DNase I \u53bb\u6c27\u6838\u7cd6\u6838\u9178\u9176I | Deoxyribonuclease I 2000 U"},"content":{"rendered":"

DNase I \u00a0\u53bb\u6c27\u6838\u7cd6\u6838\u9178\u9176I | 2000 U (1.0 ml) 2U\/ul | \u8ca8\u865fG028<\/p>\n

\u7522\u54c1\u4ecb\u7d39: DNase I\uff0c\u5373Deoxyribonuclease I\uff0c\u4e2d\u6587\u540d\u7a31\u70ba\u53bb\u6c27\u6838\u7cd6\u6838\u9178\u9176I\uff0c\u662f\u4e00\u7a2e\u53ef\u4ee5\u6d88\u5316\u55ae\u93c8\u6216\u96d9\u93c8DNA\u7522\u751f\u55ae\u53bb\u6c27\u6838\u82f7\u9178\u6216\u55ae\u93c8\u6216\u96d9\u93c8\u7684\u5be1\u53bb\u6c27\u6838\u82f7\u9178\u7684\u6838\u9178\u5167\u5207\u9176\u3002DNase I\u6c34\u89e3\u55ae\u93c8\u6216\u96d9\u93c8DNA\u5f8c\u7684\u7522\u7269\uff0c5’\u7aef\u70ba\u78f7\u9178\u57fa\u5718\uff0c3’\u7aef\u70ba\u7fa5\u57fa\u3002<\/p>\n

DNase I (2000U) | Applied Bioilogical Materials Inc. <\/p>\n

\"\"<\/p>\n

\u6d3b\u6027\u5b9a\u7fa9\uff1a<\/strong>37\u210310\u5206\u9418\u5167\uff0c\u5c07\u80fd\u5920\u5b8c\u5168\u964d\u89e31\u03bcg pBR322\u8cea\u7c92DNA\u6240\u9700\u7684\u9176\u91cf\u5b9a\u7fa9\u70ba1\u500b\u6d3b\u6027\u55ae\u4f4d\u3002<\/p>\n

\u7d14\u5ea6\uff1a<\/strong>\u4e0d\u542b\u5176\u5b83DNA40mM Tris-HCl(pH8.0)\uff0c10mM MgSO4\uff0c1mM CaCl2\uff0c1\u03bcg of pBR322 DNA\u3002\u5167\u5207\u9176\u548c\u5916\u5207\u9176\uff0c\u4e0d\u542bRNA\u9176\u3002<\/p>\n

\u9176\u5132\u5b58\u6eb6\u6db2\uff1a<\/strong>10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, and 50% (v\/v) Glycerol.\u2022 Removes DNA from protein preparations and RNA samples<\/p>\n

\u5931\u6d3b\u6216\u6291\u5236\uff1a\u52a0\u5165EDTA\u81f3\u7d42\u6fc3\u5ea6\u70ba2.5mM\u5f8c\uff0c65\u2103\u52a0\u71b110\u5206\u9418\u53ef\u4f7fDNase I\u5931\u6d3b\u3002\u915a\u6c2f\u4eff\u62bd\u63d0\u4e5f\u53ef\u4ee5\u4f7fDNase I\u5931\u6d3b\u3002\u91d1\u5c6c\u96e2\u5b50\u87af\u5408\u5291\uff0c\u9054\u5230\u6beb\u6469\u723e\/\u5347\u6fc3\u5ea6\u7684\u92c5\u96e2\u5b50\uff0c0.1%\u7684SDS\uff0cDTT\u3001\u5df0\u57fa\u4e59\u9187\u7b49\u9084\u539f\u5291\uff0c50-100mM\u4ee5\u4e0a\u9e7d\u6fc3\u5ea6\u5747\u5c0dDNase I\u6709\u986f\u8457\u6291\u5236\u4f5c\u7528\u3002<\/p>\n

\u66f4\u591a\u76f8\u95dc\u5546\u54c1<\/span><\/strong> \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a> | Agarose | \u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> | CMOS\u76f8\u6a5f<\/a> |Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nQuantity<\/strong><\/td>\nCat No.<\/strong><\/td>\n<\/tr>\n
2-Mercaptoethanol<\/a><\/td>\n100 ml<\/td>\nCH044<\/td>\n<\/tr>\n
2-Mercaptoethanol<\/a><\/td>\n200ml (2 x 100ml)<\/td>\nCH045<\/td>\n<\/tr>\n
3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs<\/a><\/td>\n50 Tabs<\/td>\nCH027<\/td>\n<\/tr>\n
3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs<\/a><\/td>\n100 Tabs<\/td>\nCH028<\/td>\n<\/tr>\n
Actinomycin D<\/a><\/td>\n5.0 mg<\/td>\nCH001<\/td>\n<\/tr>\n
Agarose<\/a><\/td>\n100 g<\/td>\nG060-1<\/td>\n<\/tr>\n
Agarose<\/a><\/td>\n500 g<\/td>\nG060-2<\/td>\n<\/tr>\n
Agarose II, Low Gelling Temperature<\/a><\/td>\n25 g<\/td>\nCH002<\/td>\n<\/tr>\n
Agarose II, Low Gelling Temperature<\/a><\/td>\n100 g<\/td>\nCH003<\/td>\n<\/tr>\n
Albumin, Bovine 20mg\/ml solution<\/a><\/td>\n1.0 ml<\/td>\nCH006<\/td>\n<\/tr>\n
Albumin, Bovine 20mg\/ml solution<\/a><\/td>\n5.0 ml<\/td>\nCH007<\/td>\n<\/tr>\n
Albumin, Bovine pH 5.1 +\/-0.2, Salt Free Form<\/a><\/td>\n25 g<\/td>\nCH004<\/td>\n<\/tr>\n
Albumin, Bovine pH 5.1 +\/-0.2, Salt Free Form<\/a><\/td>\n100 g<\/td>\nCH005<\/td>\n<\/tr>\n
Ammonium Persulfate (APS)<\/a><\/td>\n25 g<\/td>\nCH008<\/td>\n<\/tr>\n
Ammonium Persulfate (APS)<\/a><\/td>\n100 g<\/td>\nCH009<\/td>\n<\/tr>\n
Aprotinin<\/a><\/td>\n10 mg<\/td>\nCH010<\/td>\n<\/tr>\n
Aprotinin<\/a><\/td>\n50 mg<\/td>\nCH011<\/td>\n<\/tr>\n
B-Galactosidase<\/a><\/td>\n1.0 kU<\/td>\nCH036<\/td>\n<\/tr>\n
B-Galactosidase<\/a><\/td>\n5.0 kU<\/td>\nCH037<\/td>\n<\/tr>\n
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate<\/a><\/td>\n100 mg<\/td>\nCH012<\/td>\n<\/tr>\n
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate<\/a><\/td>\n500 mg<\/td>\nCH013<\/td>\n<\/tr>\n
BCIP, 5-Bromo-4-Chloro-3-Indolyl Phosphate Toluidine Salt<\/a><\/td>\n1.0 g<\/td>\nCH014<\/td>\n<\/tr>\n
BCIP\/NBT<\/a><\/td>\n100 ml<\/td>\nCH015<\/td>\n<\/tr>\n
Beef Extract Powder<\/a><\/td>\n50 g<\/td>\nCH016<\/td>\n<\/tr>\n
Beef Extract Powder<\/a><\/td>\n100 g<\/td>\nCH017<\/td>\n<\/tr>\n
Beef Extract Powder<\/a><\/td>\n500 g<\/td>\nCH018<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n5.0 g<\/td>\nG114<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n25 g<\/td>\nCH019<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n50 g<\/td>\nCH020<\/td>\n<\/tr>\n
Bromophenol Blue<\/a><\/td>\n100 g<\/td>\nCH021<\/td>\n<\/tr>\n
Bromophenol Blue, Sodium Salt<\/a><\/td>\n50 g<\/td>\nCH022<\/td>\n<\/tr>\n
Bromophenol Blue, Sodium Salt<\/a><\/td>\n100 g<\/td>\nCH023<\/td>\n<\/tr>\n
Coomassie Brilliant Blue R-250<\/a><\/td>\n10 g<\/td>\nCH024<\/td>\n<\/tr>\n
Coomassie Brilliant Blue R-250<\/a><\/td>\n25 g<\/td>\nCH025<\/td>\n<\/tr>\n
Coomassie Brilliant Blue R-250<\/a><\/td>\n50 g<\/td>\nCH026<\/td>\n<\/tr>\n
Cumate<\/a><\/td>\n500 \u03bcl<\/td>\nCH065<\/td>\n<\/tr>\n
DNA Loading Dye<\/a><\/td>\n6X; 3 x 1.0 ml<\/td>\nG030<\/td>\n<\/tr>\n
DNA Loading Green Dye<\/a><\/td>\n6X; 3 x 1.0 ml<\/td>\nG924<\/td>\n<\/tr>\n
DNA, Fish Sperm, Sodium Salt<\/a><\/td>\n5.0 g<\/td>\nCH029<\/td>\n<\/tr>\n
DNA, Fish Sperm, Sodium Salt<\/a><\/td>\n10 g<\/td>\nCH030<\/td>\n<\/tr>\n
DNA, Fish Sperm, Sodium Salt<\/a><\/td>\n25 g<\/td>\nCH031<\/td>\n<\/tr>\n
DNase I<\/a><\/td>\n2000 U (1.0 ml)<\/td>\nG028<\/td>\n<\/tr>\n
EDTA, Disodium Salt, Dihydrate<\/a><\/td>\n500 g<\/td>\nCH032<\/td>\n<\/tr>\n
EDTA, Disodium Salt, Dihydrate<\/a><\/td>\n1.0 kg<\/td>\nCH033<\/td>\n<\/tr>\n
EDTA, Disodium Salt, Dihydrate<\/a><\/td>\n2.5 kg<\/td>\nCH034<\/td>\n<\/tr>\n
Glycerol<\/a><\/td>\n500 ml<\/td>\nG115<\/td>\n<\/tr>\n
Glycerol<\/a><\/td>\n100 ml<\/td>\nCH038<\/td>\n<\/tr>\n
Glycogen<\/a><\/td>\n20 mg\/ml; 1.0 ml<\/td>\nG024<\/td>\n<\/tr>\n
Hepes, Sodium Salt<\/a><\/td>\n25 g<\/td>\nCH039<\/td>\n<\/tr>\n
Hepes, Sodium Salt<\/a><\/td>\n100 g<\/td>\nCH040<\/td>\n<\/tr>\n
Hepes, Sodium Salt<\/a><\/td>\n500 g<\/td>\nCH041<\/td>\n<\/tr>\n
IPTG<\/a><\/td>\n1.0 g<\/td>\nG116<\/td>\n<\/tr>\n
LB Agar<\/a><\/td>\n500 g<\/td>\nG247<\/td>\n<\/tr>\n
LB Broth<\/a><\/td>\n500 g<\/td>\nG246<\/td>\n<\/tr>\n
Neutral Red<\/a><\/td>\n5 x 1.0ml<\/td>\nG035<\/td>\n<\/tr>\n
Nitroblue Tetrazolium Chloride<\/a><\/td>\n500 mg<\/td>\nCH046<\/td>\n<\/tr>\n
Nitroblue Tetrazolium Chloride<\/a><\/td>\n1.0 g<\/td>\nCH047<\/td>\n<\/tr>\n
Oligo(dT)18 Primer<\/a><\/td>\n1.0OD, 500 \u03bcl, 10 \u03bcM<\/td>\nG140<\/td>\n<\/tr>\n
Pepstatin<\/a><\/td>\n5.0 mg<\/td>\nCH048<\/td>\n<\/tr>\n
Polybrene<\/a><\/td>\n1.0 ml; 0.8 mg\/ml<\/td>\nG062<\/td>\n<\/tr>\n
Proteinase K<\/a><\/td>\n1.0 ml<\/td>\nG029<\/td>\n<\/tr>\n
Random Primer (6N)<\/a><\/td>\n1.0OD, 500 \u03bcl, 10 \u03bcM<\/td>\nG139<\/td>\n<\/tr>\n
RNase A<\/a><\/td>\n25 mg<\/td>\nG117<\/td>\n<\/tr>\n
RNase A Solution<\/a><\/td>\n1.0 ml; 10 mg\/ml<\/td>\nG118<\/td>\n<\/tr>\n
RNaseOFF Ribonuclease Inhibitor<\/a><\/td>\n4,000 U (100 \u00b5l)<\/td>\nG138<\/td>\n<\/tr>\n
Sodium Azide<\/a><\/td>\n250 g<\/td>\nCH052<\/td>\n<\/tr>\n
Tetracycline, Hydrochloride<\/a><\/td>\n25 g<\/td>\nCH054<\/td>\n<\/tr>\n
Tetracycline, Hydrochloride<\/a><\/td>\n100 g<\/td>\nCH055<\/td>\n<\/tr>\n
TRIS<\/a><\/td>\n500 g<\/td>\nCH058<\/td>\n<\/tr>\n
TRIS<\/a><\/td>\n1kg (2 x 500g [CH058])<\/td>\nCH059<\/td>\n<\/tr>\n
TRIS<\/a><\/td>\n5kg (10 x 500g [CH058])<\/td>\nCH060<\/td>\n<\/tr>\n
Triton X-100, T-Octylphenoxypolyethoxyethanol<\/a><\/td>\n1L (2 x 500ml)<\/td>\nCH061<\/td>\n<\/tr>\n
Triton X-100, T-Octylphenoxypolyethoxyethanol<\/a><\/td>\n4.0 L<\/td>\nCH062<\/td>\n<\/tr>\n
TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate<\/a><\/td>\n1.0 L<\/td>\nCH063<\/td>\n<\/tr>\n
TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate<\/a><\/td>\n4.0 L<\/td>\nCH064<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

DNase I \u00a0\u53bb\u6c27\u6838\u7cd6\u6838\u9178\u9176I | 2000 U (1.0 ml) 2U\/ul | \u8ca8\u865fG028 \u7522\u54c1\u4ecb\u7d39 […]<\/p>\n","protected":false},"author":3,"featured_media":2310,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88,132],"tags":[4516],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6414"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6414"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6414\/revisions"}],"predecessor-version":[{"id":6416,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6414\/revisions\/6416"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2310"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6414"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6414"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6414"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}