{"id":6273,"date":"2016-09-01T23:39:37","date_gmt":"2016-09-01T15:39:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6273"},"modified":"2022-07-26T14:14:20","modified_gmt":"2022-07-26T06:14:20","slug":"annexin-v%e7%b4%b0%e8%83%9e%e5%87%8b%e4%ba%a1%e6%97%a9%e6%9c%9f%e6%aa%a2%e6%b8%ac-annexin-v-fitc-apoptosis-kit","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6273","title":{"rendered":"Annexin V\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u6aa2\u6e2c |Annexin V-FITC Apoptosis kit"},"content":{"rendered":"

\"BioVisionAnnexin V<\/strong>\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u6aa2\u6e2c\u5957\u7d44<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K101-400\u00a0<\/strong><\/a><\/span><\/p>\n

Annexin V-FITC Apoptosis 400<\/strong>\u500b\u5206\u6790 \u5177\u6709\u4e0d\u540c\u87a2\u5149\u4e4b\u9078\u64c7 (FITC, -Cy3, -Cy5, -EGFP, -PE, -Biotin) <\/strong><\/strong><\/p>\n

\u7d30\u80de\u51cb\u4ea1\u662f\u6307\u70ba\u7dad\u6301\u6709\u6a5f\u9ad4\u5167\u74b0\u5883\u7a69\u5b9a\uff0c\u7531\u57fa\u56e0\u63a7\u5236\u7684\u7d30\u80de\u81ea\u4e3b\u7684\u6709\u5e8f\u7684\u6b7b\u4ea1\u3002\u6b63\u5e38\u60c5\u6cc1\u4e0b\uff0c\u4efb\u4f55\u7d30\u80de\u5728\u5f62\u6210\u904e\u7a0b\u4e2d\u767c\u751f\u7684\u7570\u5e38\u90fd\u6703\u901a\u904e\u51cb\u4ea1\u6d88\u9664\u3002\u4f8b\u5982\uff0c\u9ad4\u5167\u7684\u764c\u7d30\u80de\u589e\u9577\u70ba\u816b\u7624\u7684\u904e\u7a0b\u6703\u53d7\u7d30\u80de\u51cb\u4ea1\u7684\u5f15\u5c0e\u800c\u88ab\u6291\u5236\u3002\u7136\u800c\uff0c\u5728\u6291\u764c\u57fa\u56e0p53\u51fa\u73fe\u554f\u984c\u6642\uff0c\u51cb\u4ea1\u5c31\u4e0d\u6703\u8a98\u5c0e\u767c\u751f\uff0c\u5f9e\u800c\u5c0e\u81f4\u764c\u7d30\u80de\u7684\u4e0d\u65b7\u589e\u9577\u3002\u7d30\u80de\u51cb\u4ea1\u53ef\u4ee5\u901a\u904e\u7d30\u80de\u5f62\u614b\u7684\u8b8a\u5316\u6216\u751f\u7269\u5316\u5b78\u7684\u8b8a\u5316\u4f86\u6aa2\u6e2c\u3002\u76ee\u524d\u5e38\u7528\u7684\u6307\u6a19\u6709caspase\u6d3b\u6027\u5206\u6790\u3001DNA\u7247\u6bb5\u5206\u6790\u3001\u53ca\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS)\u7684\u5916\u7ffb\u7b49\u3002Annexin V\u7d30\u80de\u67d3\u8272\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u7684\u7d30\u80de\u819c\u8b8a\u5316\u3002\u5728\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\uff0c\u819c\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS)\u7531\u8102\u819c\u5167\u5074\u7ffb\u5411\u5916\u5074\u3002Annexin V\u662f\u4e00\u7a2eCa\u96e2\u5b50\u4f9d\u8cf4\u6027\u78f7\u8102\u7d50\u5408\u86cb\u767d\uff0c\u53ef\u4ee5\u8207\u78f7\u8102\u91af\u7d72\u6c28\u9178phosphatidylserine (PS) \u7279\u7570\u7d50\u5408\u3002\u7528\u7da0\u8272\u87a2\u5149FITC\u6a19\u8a18\u7684Annexin V\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u53ef\u4ee5\u6aa2\u6e2c\u5230\u7d30\u80de\u51cb\u4ea1\u7684\u767c\u751f\u3002\u7898\u5316\u4e19\u5576(Propidium Iodide, PI)\u662f\u4e00\u7a2e\u6838\u9178\u67d3\u6599\uff0cPI\u53ea\u80fd\u900f\u904e\u51cb\u4ea1\u665a\u671f\u548c\u6b7b\u7d30\u80de\u7684\u7d30\u80de\u819c\uff0c\u56e0\u6b64Annexin V\u548cPI\u7d50\u5408\u4f7f\u7528\uff0c\u53ef\u4ee5\u5340\u5206\u51cb\u4ea1\u65e9\u665a\u671f\u7684\u7d30\u80de\u53ca\u6b7b\u7d30\u80de\u3002\u53e6\u5177\u6709\u4e0d\u540c\u87a2\u5149\u4e4b\u9078\u64c7 (<\/strong>FITC, -Cy3, -Cy5, -EGFP, -PE, -Biotin)<\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u819c\u806f\u86cb\u767dV-EGFP\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\u57fa\u65bc\u89c0\u5bdf\u5230\uff0c\u5728\u958b\u59cb\u51cb\u4ea1\u5f8c\u4e0d\u4e45\uff0c\u5927\u591a\u6578\u7d30\u80de\u985e\u578b\u5c07\u819c\u78f7\u8102\u78f7\u8102\u9170\u7d72\u6c28\u9178\uff08PS\uff09\u5f9e\u8cea\u819c\u7684\u5167\u8868\u9762\u8f49\u79fb\u5230\u7d30\u80de\u8868\u9762\u3002 \u4e00\u65e6\u5728\u7d30\u80de\u8868\u9762\u4e0a\uff0cPS\u53ef\u4ee5\u901a\u904e\u7528\u589e\u5f37\u7684\u7da0\u8272\u87a2\u5149\u86cb\u767d\uff08EGFP\uff09\u878d\u5408\u7684\u819c\u806f\u86cb\u767dV\uff08\u4e00\u7a2e\u5c0dPS\u5177\u6709\u5f37\u70c8\u7684\u5929\u7136\u89aa\u548c\u529b\u7684\u86cb\u767d\uff09\u9032\u884c\u67d3\u8272\u800c\u5bb9\u6613\u5730\u6aa2\u6e2c\u5230\u3002 \u4e00\u6b65\u67d3\u8272\u7a0b\u5e8f\u53ea\u970010\u5206\u9418\u3002 \u6b64\u5916\uff0c\u8a72\u6e2c\u5b9a\u53ef\u4ee5\u76f4\u63a5\u5728\u6d3b\u7d30\u80de\u4e0a\u9032\u884c\u3002 \u6aa2\u6e2c\u53ef\u4ee5\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u7528FITC\u904e\u6ffe\u5668\u7684\u87a2\u5149\u986f\u5fae\u93e1\u9032\u884c\u5206\u6790\u3002 EGFP\u6bd4\u5176\u4ed6\u87a2\u5149\u8a66\u5291\u66f4\u4eae\uff0c\u66f4\u7a69\u5b9a\u3002 \u7576\u9032\u884c\u819c\u806f\u86cb\u767dV-EGFP\u548cPI\u67d3\u8272\u6642\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u5340\u5206\u51cb\u4ea1\u8207\u58de\u6b7b\u3002<\/p>\n\n\n\n\n\n\n\n
Detection Method<\/th>\nFlow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy<\/td>\n<\/tr>\n
Species Reactivity<\/th>\nMammalian<\/td>\n<\/tr>\n
Applications<\/th>\nDetect early\/middle stages of apoptosis; differentiate apoptosis from necrosis.<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 Simple one step staining procedure in 10 minutes
\n\u2022 Fast and convenient
\n\u2022 EGFP is a bright and photo-stable green fluorescent protein and the kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 Annexin V-EGFP
\n\u2022 1X Binding Buffer
\n\u2022 Propidium Iodide (PI)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K101-100<\/td>\n\u00a0Annexin V-FITC Apoptosis Kit<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K101-25<\/td>\n\u00a0Annexin V-FITC Apoptosis Kit<\/a><\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
\u00a0K101-400<\/td>\n\u00a0Annexin V-FITC Apoptosis Kit<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a0K201-100<\/td>\n\u00a0Annexin V-FITC Apoptosis Kit Plus<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K201-25<\/td>\n\u00a0Annexin V-FITC Apoptosis Kit Plus<\/a><\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
\u00a0K201-400<\/td>\n\u00a0Annexin V-FITC Apoptosis Kit Plus<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a01001-1000<\/td>\n\u00a0Annexin V-FITC Reagent<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a01001-200<\/td>\n\u00a0Annexin V-FITC Reagent<\/a><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
K102-100<\/td>\n\u00a0Annexin V-Cy3 Apoptosis Kit<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K102-25<\/td>\n\u00a0Annexin V-Cy3 Apoptosis Kit<\/a><\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
\u00a0K102-400<\/td>\n\u00a0Annexin V-Cy3 Apoptosis Kit<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a0K202-100<\/td>\n\u00a0Annexin V-Cy3 Apoptosis Kit Plus<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K202-25<\/td>\n\u00a0Annexin V-Cy3 Apoptosis Kit Plus<\/a><\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
\u00a0K202-400<\/td>\n\u00a0Annexin V-Cy3 Apoptosis Kit Plus<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a01002-1000<\/td>\n\u00a0Annexin V-Cy3 Reagent<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a01002-200<\/td>\n\u00a0Annexin V-Cy3 Reagent<\/a><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K104-100<\/td>\n\u00a0Annexin V-EGFP Apoptosis Kit<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K104-25<\/td>\n\u00a0Annexin V-EGFP Apoptosis Kit<\/a><\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
\u00a0K104-400<\/td>\n\u00a0Annexin V-EGFP Apoptosis Kit<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a01004-1000<\/td>\n\u00a0Annexin V-EGFP Reagent<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a01004-200<\/td>\n\u00a0Annexin V-EGFP Reagent<\/a><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a01056-1<\/td>\n\u00a0Propidium Iodide <\/a><\/td>\n\u00a01 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Annexin V\u7d30\u80de\u51cb\u4ea1\u65e9\u671f\u6aa2\u6e2c\u5957\u7d44 | \u8ca8\u865fK101-400\u00a0 Annexin V-FITC Apopto […]<\/p>\n","protected":false},"author":3,"featured_media":6274,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[190,84,3],"tags":[4542],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6273"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6273"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6273\/revisions"}],"predecessor-version":[{"id":14277,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6273\/revisions\/14277"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6274"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6273"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6273"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6273"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}