{"id":6238,"date":"2016-09-01T15:22:35","date_gmt":"2016-09-01T07:22:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6238"},"modified":"2022-07-26T14:38:29","modified_gmt":"2022-07-26T06:38:29","slug":"absolute-antibody%e7%b3%bb%e5%88%97-anti-vaccinia-l1-protein-m12b9-fab-%e6%8a%97%e9%ab%94","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6238","title":{"rendered":"Absolute Antibody\u7cfb\u5217 | Anti-Vaccinia L1 protein [M12b9 Fab] \u6297\u9ad4"},"content":{"rendered":"

Fc Silent\u2122 Antibodies: Turning down the background noise<\/strong><\/p>\n

Fc Silent\u00a0<\/strong>\u6297\u9ad4<\/strong> | <\/strong>\u53ea\u8981<\/strong>\u8a0a<\/strong>\u865f\u4e0d\u8981\u96dc<\/strong>\u8a0a (Engineered Fc Domain)<\/strong><\/span><\/p>\n

Absolute Antibody \u63d0\u4f9b\u4e00\u7cfb\u5217\u6297\u9ad4\u5546\u54c1\uff0c\u5176\u4e2dFc Silent\u2122 Antibodies\u7cfb\u5217\uff1b\u80fd\u6709\u6548\u964d\u4f4e\u975e\u7279\u7570\u6027Fc\u7d50\u5408\u3002Fc Silent\u2122\u6280\u8853\uff0c\u70ba\u61c9\u7528\u751f\u7269\u5de5\u7a0b\u6280\u8853\uff0c\u5c07Fc receptors (Fc\u03b3R, FcR)\u9ede\u7a81\u8b8a\uff0c\u9032\u800c\u4e2d\u6b62\u6297\u9ad4\u8207ADCC\u7684\u529f\u80fd\uff1b\u6709\u6548\u964d\u4f4eFc\u6240\u9020\u6210\u7684\u80cc\u666f\u8a0a\u865f\u3002<\/p>\n

Fc Silent\u2122\u6297\u9ad4\u61c9\u7528\u65bc\u514d\u75ab\u7d30\u80de\u6642flow cytometry\u3001immunohistochemistry (IHC)\u8cc7\u6599\u986f\u793a\uff0c\u6709\u6548\u964d\u4f4e\u975e\u7279\u7570\u6027\u4e4bFc\u7d50\u5408\uff0c\u6240\u9020\u6210\u7684\u80cc\u666f\u503c\u3002(\u8a02\u8cfc\u6297\u9ad4\u524d\uff0c\u8acb\u78ba\u8a8d\u9078\u64c7\u6297\u9ad4\u662f\u5426\u6709Fc Silent\u2122\u4fee\u98fe\u6a19\u8a8c)<\/p>\n

\"f480_silent_post\"<\/p>\n

\n

Anti-Vaccinia L1 protein [M12b9 Fab] \u6297\u9ad4 | \u7522\u54c1\u63cf\u8ff0<\/p>\n

Recombinant monoclonal antibody to Vaccinia L1 protein. Manufactured using AbAb\u2019s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma M12b9 Fab.<\/p>\n\n\n\n\n\n\n\n
\u6297\u9ad4\u540d\u7a31<\/td>\nAnti-Vaccinia L1 protein [M12b9 Fab]<\/td>\n<\/tr>\n
\u6297\u9ad4\u8ca8\u865f<\/td>\nAb00775-2.0 \/ Ab00775-2.3 \/ Ab00775-1.6 \/ Ab00775-23.0<\/td>\n\u7279\u7570\u6027This fab is specific for a conformational epitope on the vaccina virus L1 protein. L1 is a myristoylated, transmembrane protein found on the surface of the mature virion that plays a key role in the formation of infectious virus. L1 is conserved in all orthopoxviruses, and its sequence is almost identical among vaccinia virus, Variola virus and monkeypox virus.<\/td>\n<\/tr>\n
This antibody was prepared by intranasally infecting BALB\/c mice with an attenuated vaccinia virus (VACV) mutant, followed by a boost with an intravenous injection of UV-inactivated WT VACV.<\/td>\n<\/tr>\n
\u514d\u75ab\u539f<\/td>\nThis Fab was generated by papain digestion of purified M12B9, a murine anti-vaccinia L1 mAb, which was raised by immunizing mice with live vaccinia virus (VACV) and refolded L1 protein.<\/td>\n<\/tr>\n
\u61c9\u7528\u6ce8\u610f<\/td>\nThis Fab forms a stable complex with L1 protein, and consequently has been used in X-ray crystallography studies to determine the complex’s structure and the mode of Fab binding to L1 (Kaever, 2014). This Fab has been shown to bind to four long loops that connect the 4-helix bundle at the tip of L1, in an orientation that is very similar to that of the antibody 7D11. In addition, this Fab has been used in biolayer interferometry (BLI)-based assays.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n
Product<\/a><\/strong><\/td>\nExpected Species Reactivity<\/a><\/strong><\/td>\nIsotype\/Format<\/a><\/strong><\/td>\nModification<\/a><\/strong><\/td>\n<\/tr>\n<\/thead>\n
Ab00775-2.0 Anti-Vaccinia L1 protein [M12b9 Fab]<\/a><\/td>\nVaccinia virus<\/td>\nMouse IgG2a<\/td>\n<\/td>\n<\/tr>\n
Ab00775-2.3 Anti-Vaccinia L1 protein [M12b9 Fab]<\/a><\/td>\nVaccinia virus<\/td>\nMouse IgG2a<\/td>\nFc Silent\u2122<\/a><\/td>\n<\/tr>\n
Ab00775-1.6 Anti-Vaccinia L1 protein [M12b9 Fab]<\/a><\/td>\nVaccinia virus<\/td>\nMouse Fab fragment<\/td>\nHis-Tagged<\/td>\n<\/tr>\n
Ab00775-23.0 Anti-Vaccinia L1 protein [M12b9 Fab]<\/a><\/td>\nVaccinia virus<\/td>\nRabbit IgG<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Fc Silent\u2122 Antibodies: Turning down the background nois […]<\/p>\n","protected":false},"author":3,"featured_media":6231,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[4552],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6238"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6238"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6238\/revisions"}],"predecessor-version":[{"id":6239,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6238\/revisions\/6239"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6231"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6238"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6238"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6238"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}