{"id":6230,"date":"2016-09-01T14:38:19","date_gmt":"2016-09-01T06:38:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6230"},"modified":"2022-07-26T14:38:46","modified_gmt":"2022-07-26T06:38:46","slug":"absolute-antibody%e7%b3%bb%e5%88%97-anti-a14-bb5-2vaccinia-virus","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6230","title":{"rendered":"Absolute Antibody\u7cfb\u5217 | Anti-A14 Vaccinia virus"},"content":{"rendered":"

Fc Silent\u2122 Antibodies: Turning down the background noise<\/strong><\/p>\n

Fc Silent\u00a0<\/strong>\u6297\u9ad4<\/strong> | <\/strong>\u53ea\u8981<\/strong>\u8a0a<\/strong>\u865f\u4e0d\u8981\u96dc<\/strong>\u8a0a (Engineered Fc Domain)<\/strong><\/span><\/p>\n

Absolute Antibody \u63d0\u4f9b\u4e00\u7cfb\u5217\u6297\u9ad4\u5546\u54c1\uff0c\u5176\u4e2dFc Silent\u2122 Antibodies\u7cfb\u5217\uff1b\u80fd\u6709\u6548\u964d\u4f4e\u975e\u7279\u7570\u6027Fc\u7d50\u5408\u3002Fc Silent\u2122\u6280\u8853\uff0c\u70ba\u61c9\u7528\u751f\u7269\u5de5\u7a0b\u6280\u8853\uff0c\u5c07Fc receptors (Fc\u03b3R, FcR)\u9ede\u7a81\u8b8a\uff0c\u9032\u800c\u4e2d\u6b62\u6297\u9ad4\u8207ADCC\u7684\u529f\u80fd\uff1b\u6709\u6548\u964d\u4f4eFc\u6240\u9020\u6210\u7684\u80cc\u666f\u8a0a\u865f\u3002<\/p>\n

Fc Silent\u2122\u6297\u9ad4\u61c9\u7528\u65bc\u514d\u75ab\u7d30\u80de\u6642flow cytometry\u3001immunohistochemistry (IHC)\u8cc7\u6599\u986f\u793a\uff0c\u6709\u6548\u964d\u4f4e\u975e\u7279\u7570\u6027\u4e4bFc\u7d50\u5408\uff0c\u6240\u9020\u6210\u7684\u80cc\u666f\u503c\u3002(\u8a02\u8cfc\u6297\u9ad4\u524d\uff0c\u8acb\u78ba\u8a8d\u9078\u64c7\u6297\u9ad4\u662f\u5426\u6709Fc Silent\u2122\u4fee\u98fe\u6a19\u8a8c)<\/p>\n

\"f480_silent_post\"<\/p>\n

\n

Anti-A14 [BB5.2] \u6297\u9ad4 | \u7522\u54c1\u63cf\u8ff0:<\/p>\n

Recombinant monoclonal antibody to A14. Manufactured using AbAb\u2019s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BB5.2.<\/p>\n\n\n\n\n\n\n\n
\u6297\u9ad4\u540d\u7a31<\/td>\n\n

Recombinant monoclonal antibody to A14.<\/p>\n

Vaccinia virus<\/td>\n

\u76f8\u7570\u540d\u7a31Vaccinia virus A14 phosphoprotein; virion membrane protein<\/td>\n<\/tr>\n
\u6297\u9ad4\u8ca8\u865f<\/td>\nAb00733-1.1 Anti-A14 [BB5.2]<\/td>\n\u7279\u7570\u6027This antibody is specific for the vaccinia virus A14 phosphoprotein, which is expressed on the membranes of intracellular mature virions.<\/td>\n<\/tr>\n
Recombinant monoclonal antibody to A14. Manufactured using AbAb\u2019s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BB5.2.<\/td>\n<\/tr>\n
\u514d\u75ab\u539f<\/td>\nThis antibody was produced by infecting a BALB\/c mouse intranasally with WR.K1L\u2013C7L\u2013, a highly attentuated vaccinia virus. The mouse was subsequently challenged with intranasal inoculation of of wild type VACV, followed by intravenous injection with UV-inactivated VACV<\/td>\n<\/tr>\n
\u61c9\u7528\u6ce8\u610f<\/td>\nA14 was among one of a group of VAVC proteins that elicited strong polyclonal antibody responses, resulting in multiple clones of hybridomas being generated against the same protein, all of which were analysed with IF, IP and WB. These clones have not, as of yet, been individually described in published literatu<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u66f4\u591a\u76f8\u95dc\u6297\u9ad4Primary Antibodies<\/p>\n

\n
\n
\n
\n
\n
\n\n\n\n\n\n\n\n
Product Name page<\/a><\/th>\nExpected Species Reactivity<\/a><\/th>\nApplications<\/a><\/th>\n

<\/tr>\n<\/thead>\n

Anti-A14 [BB5.2]<\/a><\/td>\nVaccinia virus<\/td>\nWB; IP; IF<\/td>\n

<\/tr>\n

Anti-Vaccinia A33 glycoprotein [A2C7]<\/a><\/td>\nVaccinia virus<\/td>\nIF; neutralization<\/td>\n

<\/tr>\n

Anti-Vaccinia L1 protein [M12b9 Fab]<\/a><\/td>\nVaccinia virus<\/td>\ncrystallization; BLI binding assay<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/article>\n<\/div>\n<\/div>\n<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Fc Silent\u2122 Antibodies: Turning down the background nois […]<\/p>\n","protected":false},"author":3,"featured_media":6231,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[90,136,3],"tags":[4554],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6230"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6230"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6230\/revisions"}],"predecessor-version":[{"id":6246,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6230\/revisions\/6246"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6231"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6230"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6230"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}