{"id":6152,"date":"2016-08-31T14:11:26","date_gmt":"2016-08-31T06:11:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6152"},"modified":"2022-07-26T14:43:27","modified_gmt":"2022-07-26T06:43:27","slug":"lentivirus-%e7%b4%94%e5%8c%96%e8%88%87%e6%bf%83%e5%ba%a6%e5%a5%97%e7%b5%84-plus-%e6%8f%90%e9%ab%98%e7%97%85%e6%af%92%e7%b4%94%e5%ba%a6%e5%8f%8a%e5%8a%9b%e5%83%b9-%e8%b2%a8%e8%99%9flv998","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6152","title":{"rendered":"Lentivirus \u7d14\u5316\u8207\u6fc3\u5ea6\u5957\u7d44 plus |\u63d0\u9ad8\u75c5\u6bd2\u7d14\u5ea6\u53ca\u529b\u50f9 \u8ca8\u865fLV998"},"content":{"rendered":"<p><strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6152\">\u3010\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<sup> ++plus<\/sup>\u3011Ultra-Pure Lentivirus Purification Kit \u6162\u75c5\u6bd2\u7d14\u5316\u53ca\u6fc3\u5ea6\u5957\u7d44<\/a><\/span>\uff0c\u6709\u6548\u63d0\u9ad8\u75c5\u6bd2\u7d14\u5ea6\u53ca\u529b\u50f9 (Cat. No. LV998)<\/strong><\/p>\n<p>\u91cd\u7d44\u6162\u75c5\u6bd2lentivirus\uff0c\u5ee3\u6cdb\u61c9\u7528\u65bc\u7d30\u80de\u4e4b\u8f49\u5c0e\u3002\u8f49\u67d3\u5f8c\u7684\u75c5\u6bd2\u4e0a\u6e05\u6db2\u529b\u50f9\uff0c\u7d0410<sup>6<\/sup> CFU\u83cc\u843d\u5f62\u6210\u55ae\u4f4d\uff08\u83cc\u843d\u5f62\u6210\u55ae\u4f4d\uff09\/ ml\u3002\u5728\u9ad8\u6548\u80fd\u75c5\u6bd2\u529b\u50f9\u53ca\u9ad4\u5167\u57fa\u56e0\u50b3\u905e\u4e2d\uff0c\u8981\u6c42\u8f03\u9ad8\u7684\u75c5\u6bd2\u7d14\u5ea6\u53ca\u529b\u50f9\uff0c\u4e14\u65bc\u6d3b\u9ad4\u8a66\u9a57\u4e2d\uff0c\u907f\u514d\u75c5\u6bd2\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\uff0c\u6240\u9020\u6210\u4e4b\u6c61\u67d3\u53ef\u80fd\u6027\u3002\u56e0\u6b64\uff0c\u75c5\u6bd2\u4e0a\u6e05\u9700\u8981\u9032\u4e00\u6b65\u6fc3\u7e2e\u53ca\u7d14\u5316\u3002Abmgood\u63d0\u4f9b\u4e00\u5957\u75c5\u6bd2\u7d14\u5316\u5957\u7d44\u3002\u50b3\u7d71\u7d14\u5316\u75c5\u6bd2\uff0c\u7d93\u7531\u8d85\u901f\u96e2\u5fc3\u6fc3\u7e2e\u3002\u96d6\u7136\u8a72\u65b9\u6cd5\u6548\u679c\u5f88\u597d\uff0c\u4f46\u9700\u8981\u7279\u5b9a\u7684\u7684\u8d85\u901f\u96e2\u5fc3\u8a2d\u5099\uff0c\u4e14\u6280\u8853\u8981\u6c42\u9ad8\u3002\u6b64\u5916\uff0c\u4ee5\u6fc3\u7e2e\u7684\u75c5\u6bd2\u4e0a\u6e05\u7e3d\u9ad4\u7a4d\u662f\u6709\u9650\u7684\u8d85\u901f\u96e2\u5fc3\u7ba1\u7684\u5927\u5c0f\u3002\u5831\u544a\u6307\u51fa\uff0c\u8d85\u901f\u96e2\u5fc3\u904e\u7a0b\u4e2d\uff0c\u6703\u9020\u6210\u4e00\u4e9b\u4e0d\u5229\u7684\u7269\u7406\u6548\u61c9\uff0c\u6703\u5f71\u97ff\u75c5\u6bd2\u9846\u7c92\u7684\u751f\u7269\u6d3b\u6027\u3002<\/p>\n<p>Abmgood\u767c\u5c55 Ultra-Pure Lentivirus Purification Kit (Cat. No.LV998).<\/p>\n<p>\u91dd\u5c0d\u75c5\u6bd2\u6fc3\u7e2e\u6280\u8853\u53ca\u7d14\u5ea6\u505a\u6539\u826f\u3002\u63d0\u4f9b\u4e00\u500b\u5feb\u901f\u4e14\u6709\u6548\uff0c\u7d14\u5316\u53ca\u6fc3\u7e2e\u75c5\u6bd2\u7684\u7522\u54c1\u3002\u5176\u8a2d\u8a08\u539f\u7406\uff0c\u70ba\u5229\u7528\u96e2\u5b50\u4ea4\u63db\u7684\u7d50\u5408\u548c\u7368\u7279\u7de9\u885d\u6db2\uff0c\u6700\u5927\u9650\u5ea6\u6e1b\u5c11\u75c5\u6bd2\u9846\u7c92\u7684\u640d\u58de\uff0c\u4e14\u63d0\u9ad8\u75c5\u6bd2\u529b\u50f9\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"83\">LV999<\/td>\n<td width=\"261\"><a href=\"http:\/\/www.abmgood.com\/PEG-Lentivirus-Purification-Concentration.html\" target=\"_blank\">Speedy Lentivirus Purification<\/a><\/td>\n<td style=\"text-align: center;\" width=\"100\">100ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"83\">LV900<\/td>\n<td width=\"261\"><a href=\"http:\/\/www.abmgood.com\/High-Titer-Lentivirus-Calculation.html\" target=\"_blank\">qPCR Lentivirus Titration(Titer) Kit<\/a><\/td>\n<td style=\"text-align: center;\" width=\"100\">100 Reactions<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"83\">LV998<\/td>\n<td width=\"261\"><a href=\"http:\/\/www.abmgood.com\/Ultra-Lentivirus-Purification-Concentration.html\" target=\"_blank\">Ultra-Pure Lentivirus Purification Kit<\/a>\uff0d\u53ef\u7d14\u5316\u75c5\u6bd2\u6fc3\u5ea6\u905410<sup>11<\/sup>\u75c5\u6bd2\u9846\u7c92<\/td>\n<td style=\"text-align: center;\" width=\"100\">1 Kit<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" rowspan=\"2\" width=\"83\">G171<br \/>\nG172<br \/>\nG173<\/td>\n<td rowspan=\"2\" width=\"261\"><a href=\"http:\/\/www.abmgood.com\/Lentivirus-Purification-Concentration.html\" target=\"_blank\">PuRetro\u2122 Lentivirus Purification Kit<\/a><\/td>\n<td style=\"text-align: center;\" rowspan=\"2\" width=\"100\">2 Standard Units<br \/>\n5 Standard Units<br \/>\n2 Giga Units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"attachment-266x266 alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/08\/\u672a\u547d\u540d-14.jpg\" alt=\"\u672a\u547d\u540d-14\" width=\"68\" height=\"23\" \/><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6146\">\u3010<\/a><\/span><\/strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6146\"><strong>\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316\u3011<\/strong> <strong>ABM Speedy Lentivirus Purification kit <\/strong><strong>\u6162<\/strong><strong>\u75c5\u6bd2<\/strong><strong>\u4f4e<\/strong><strong>\u901f30<\/strong><strong>\u5206<\/strong><strong>\u7d14\u5316\u5957\u7d44 (100<\/strong><strong>\u500d\u6fc3\u7e2e<\/strong><strong>)<\/strong><strong> \u00a0(Cat. No. LV999)<\/strong><\/a><\/span><\/p>\n<p>\u50b3\u7d71\u7684\u75c5\u6bd2\u7d14\u5316\u65b9\u6cd5\uff0c\u5927\u591a\u5229\u7528\u8d85\u9ad8\u901f\u96e2\u5fc3(ultracentrifuge)\uff0c\u642d\u914d\u6fc3\u5ea6\u68af\u5ea6(CsCl)\u7b49\u65b9\u6cd5\u9032\u884c\u75c5\u6bd2\u5206\u96e2\uff0c\u9700\u8981\u7d14\u719f\u6280\u8853\u4e14\u904e\u7a0b\u8cbb\u6642\u3002Speedy Lentivirus purification kti\u75c5\u6bd2\u7d14\u5316\u5957\u7d44\u53ef\u514d\u9664\u4f7f\u7528\u8d85\u9ad8\u901f\u96e2\u5fc3\uff0c\u66f4\u7c21\u55ae\u3001\u5feb\u901f\u53d6\u5f97\u9ad8\u6fc3\u5ea6\u75c5\u6bd2\u6db2\u300230\u5206\u9418\u5167\u5feb\u901f\u7d14\u5316\u75c5\u6bd2\u3001\u53ef\u9054\u6fc3\u7e2e\u6548\u679c\u7d04100x\u3002<\/p>\n<p>\u200b<img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/ABM_Speedy.gif\" alt=\"ABM_Speedy\" width=\"251\" height=\"118\" \/><\/p>\n<p>293 cells \u611f\u67d3\u7d14\u5316\u524d\uff08A\uff09\u8207\u7d14\u5316\u5f8c\uff08B\uff09\u7684lentivirus 48hr\u5f8c\u7684\u7d50\u679c<\/p>\n<p>&nbsp;<\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"attachment-266x266 alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/08\/\u672a\u547d\u540d-14.jpg\" alt=\"\u672a\u547d\u540d-14\" width=\"68\" height=\"23\" \/><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6156\">\u3010<\/a><\/span><\/strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6156\"><strong>\u75c5\u6bd2<\/strong><strong>RT-QPCR<\/strong><strong>\u5b9a\u91cf\u3011<\/strong><strong>ABM qPCR Lentivirus Titration(Titer) Kit<\/strong> <strong>Lentivirus<\/strong><strong>\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf) (Cat. No. LV900)<\/strong><\/a><\/span><\/p>\n<p>Abmgood\u63d0\u4f9b\u5feb\u901f\u4e14\u65b9\u4fbf\u4e4bLentivirus\u75c5\u6bd2\u529b\u50f9\u5b9a\u91cf\u5957\u7d44\u3002\u5176\u7d50\u5408\u4e00\u500b\u6b65\u9a5f\u5feb\u901fRNA\u8403\u53d6\u53caQPCR\u6280\u8853 (SYBR system)\uff0c2\u500b\u5c0f\u6642\u5b8c\u6210\u5b9a\u91cf\u3002\u5957\u7d44\u5167\u9644\u6709reagent mix, \u53caprimer\u3002primer\u4ee5HIV based vetors\u70ba\u8a2d\u8a08\u7684\u5075\u6e2c\u65b9\u5f0f\u3002\u5feb\u901f\u5b8c\u6210\u75c5\u6bd2\u529b\u50f9\u5b9a\u91cf\u5206\u6790\u3002\u4e0d\u6703\u6709NTC\u91cf\u5316\u554f\u984c\uff0c\u5b8c\u5168\u6392\u9664NTC\u8a0a\u865f\u3002\u7cbe\u78ba\u5b9a\u91cfLentivirus titer\u4e4b\u65b9\u6cd5\u3002\u6a19\u6e96\u54c1STD\u70balentivirus\u75c5\u6bd2\u3002Standard\u6fc3\u5ea6\u70ba\u00a05x 10^7 IU\/ml. Standard 2\u00a0\u6fc3\u5ea6\u70ba6.25&#215;10^6 IU\/ml.\u00a0\u5169\u8005standards\u00a0\u76f8\u5dee\u00a02^3,\u00a0\u76f8\u7576\u65bcCt\u00a0\u503c3\u6b21\u65b9\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/viralexp\/images\/titerkit.png\" alt=\"\" width=\"385\" height=\"103\" \/><\/p>\n<ol>\n<li>One-Step qPCR\uff0c2\u5c0f\u6642\u5167\u53ef\u5b8c\u6210\u5b9a\u91cf\u3002<\/li>\n<li>Primer\u7d93\u7279\u6b8a\u8a2d\u8a08\uff0c\u89e3\u6c7aprimer dimer\u5e72\u64fe\u3002<\/li>\n<li>\u9644\u6709\u75c5\u6bd2\u6fc3\u5ea6\u6a19\u6e96\u6db2\u5169\u7d44\uff0c\u6e96\u78ba\u5b9a\u91cf\u75c5\u6bd2\u6fc3\u5ea6\u3002<\/li>\n<li>\u642d\u914d<u><a href=\"http:\/\/biopioneer.com.tw\/?p=2197\" data-cke-saved-href=\"http:\/\/biopioneer.com.tw\/?p=2197\">ABM qPCR Mastermix<\/a><\/u>\uff0c\u6548\u679c\u66f4\u4f73\u3002<\/li>\n<\/ol>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"attachment-266x266 alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/08\/\u672a\u547d\u540d-14.jpg\" alt=\"\u672a\u547d\u540d-14\" width=\"68\" height=\"23\" \/>\u3010<strong>\u75c5\u6bd2<\/strong><strong>\u5feb\u7be9\u5b9a\u91cf<\/strong><strong>\u3011<\/strong><\/strong>\u00a0<strong>Instant Lentivirus Titration Card<\/strong><strong>\u6162\u75c5\u6bd2\u5b9a\u91cf\u5361\/<\/strong><strong>\u5feb\u7be9\u5f0f\u6aa2\u6e2c<\/strong><strong>(Cat. No. G614\/G615)<\/strong><\/p>\n<p><strong>\u00a0<img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/viralexp\/images\/Lenti-Card-figure-1.jpg\" alt=\"\" width=\"325\" height=\"255\" \/><\/strong><\/p>\n<p>\u4f7f\u7528\u6a6b\u5411\u6d41\u65b9\u6cd5\uff08lateral flow immunoassay\uff09\u5075\u6e2clentiviral capsule protein\u9032\u884c\u5b9a\u91cf\u3002\u6bd4\u8d77\u50b3\u7d71infection based assays\u97003-4\u5929\uff0cInstant Lentivirus Titration Card \u53ea\u898110\u5206\u9418\u5373\u53ef\u5f97\u5230\u5b9a\u91cf\u7d50\u679c\u3002\u7c21\u55ae\u64cd\u4f5c\uff0c10\u5206\u9418\u7372\u5f97\u7d50\u679c\uff0d\u4e0d\u9700\u984d\u5916\u8403\u53d6\u6b65\u9a5f\uff1b\u76ee\u6e2c\u89c0\u5bdf\u7d50\u679c\u984f\u8272\u8b8a\u5316\u00a0\u7528\u9014\uff1a\u8a55\u4f30\u57f9\u990a\u6db2\u4e2d\u9069\u7576\u7684\u75c5\u6bd2\u6fc3\u5ea6\u3002\u9748\u654f\u5ea6\u53ef\u90541\u00d710<sup>5<\/sup>\u00a0IU\/mL\u75c5\u6bd2\u6fc3\u5ea6\uff08\u62167.7 pg\/ml\u7684p24 viral capsid protein\uff09<\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"attachment-266x266 alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/08\/\u672a\u547d\u540d-14.jpg\" alt=\"\u672a\u547d\u540d-14\" width=\"68\" height=\"23\" \/><\/strong><\/p>\n<p><a style=\"color: #800080;\" href=\"http:\/\/biopioneer.com.tw\/?p=6146\">Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | <a style=\"color: #800080;\" href=\"http:\/\/biopioneer.com.tw\/?p=6152\">Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| <a style=\"color: #800080;\" href=\"http:\/\/biopioneer.com.tw\/?p=6159\">Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | <a style=\"color: #800080;\" href=\"http:\/\/biopioneer.com.tw\/?p=6156\">Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316 ++plus\u3011Ultra-Pure Lentivirus Purification Kit  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":6153,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[454,57,116,456],"tags":[4569],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6152"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6152"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6152\/revisions"}],"predecessor-version":[{"id":10615,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6152\/revisions\/10615"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6153"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6152"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6152"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6152"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}