{"id":6146,"date":"2016-08-31T13:42:31","date_gmt":"2016-08-31T05:42:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6146"},"modified":"2025-07-01T13:56:05","modified_gmt":"2025-07-01T05:56:05","slug":"lentivirus%e6%85%a2%e7%97%85%e6%af%92-%e4%bd%8e%e9%80%9f30%e5%88%86%e7%b4%94%e5%8c%96%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9flv999","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=6146","title":{"rendered":"Lentivirus\u6162\u75c5\u6bd2\u7d14\u5316 |\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44 \u8ca8\u865fLV999"},"content":{"rendered":"

Lentivirus\u6162\u75c5\u6bd2\u7d14\u5316 |\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44 \u8ca8\u865fLV999 <\/span><\/strong><\/span><\/a><\/span><\/p>\n

\u91cd\u7d44\u6162\u75c5\u6bd2\u8f09\u9ad4(Recombinant lentiviral vectors) \u5df2\u88ab\u8b49\u660e\u662f\u7a69\u5b9a\u57fa\u56e0\u8f49\u79fb(stable gene transfer)\u7684\u6709\u529b\u5de5\u5177\u9ad4\u5916(in vitro)\u548c\u9ad4\u5167(in vivo)\u5206\u88c2(dividing)\u548c\u975e\u5206\u88c2\u7d30\u80de(non-dividing cells)\u3002\u901a\u904e\u591a\u5e74\u7684\u7d93\u9a57\u8207\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors)\uff0cabmgood \u958b\u767c\u4e86\u81ea\u5df1\u5c08\u6709\u7684 pLenti-combo \u5305\u88dd\u6df7\u5408\u7269(pLenti-combo packaging mix)\u548c\u4e00\u7a2e\u5feb\u901f\u751f\u7522\u91cd\u7d44\u6162\u75c5\u6bd2\u8f09\u9ad4(recombinant lentiviral vectors)\u7684\u6709\u6548\u65b9\u6848\uff0c\u5176\u6ef4\u5ea6(titers)\u9ad8\u9054107<\/sup>\u570b\u969b\u55ae\u4f4d\/\u6beb\u5347\u3002\u5feb\u6377\u3001\u7c21\u55ae\u7d14\u5316(purify)\u548c\u6fc3\u7e2e(concentrate)\u6162\u75c5\u6bd2\u9846\u7c92\u8fd1100 \u500d\u3002<\/p>\n

<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

\u3010<\/strong>\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316\u3011<\/strong> \u6162<\/strong>\u75c5\u6bd2<\/strong>\u4f4e<\/strong>\u901f30<\/strong>\u5206<\/strong>\u7d14\u5316\u5957\u7d44 (100<\/strong>\u500d\u6fc3\u7e2e<\/strong>)<\/strong> \u00a0(Cat. No. LV999)<\/strong><\/p>\n

\u50b3\u7d71\u7684\u75c5\u6bd2\u7d14\u5316\u65b9\u6cd5\uff0c\u5927\u591a\u5229\u7528\u8d85\u9ad8\u901f\u96e2\u5fc3(ultracentrifuge)\uff0c\u642d\u914d\u6fc3\u5ea6\u68af\u5ea6(CsCl)\u7b49\u65b9\u6cd5\u9032\u884c\u75c5\u6bd2\u5206\u96e2\uff0c\u9700\u8981\u7d14\u719f\u6280\u8853\u4e14\u904e\u7a0b\u8cbb\u6642\u3002Speedy Lentivirus purification KIT\u5feb\u901f\u6162\u75c5\u6bd2\u7d14\u5316\u662f\u5e36\u6b63\u96fb\u7684\u57fa\u8cea\u6eb6\u6db2\uff0c\u8207\u5e36\u8ca0\u96fb\u7684\u6162\u75c5\u6bd2\u7d50\u5408\uff0c\u5f62\u6210\u6c89\u6fb1\uff0c\u53ef\u901a\u904e\u4f4e\u901f\u96e2\u5fc3\u6c89\u6fb1\u3002\u53ef\u76f4\u63a5\u61c9\u7528\u65bcFBS \u53cacalcium\u542b\u91cf\u4e4b\u75c5\u6bd2\u6db2\u6fc3\u7e2e\uff0c\u4e0d\u5f71\u97ff\u5176 \u7d14\u5316\u4e4b\u6548\u80fd\u3002 Speedy Lentivirus purification KIT\u75c5\u6bd2\u7d14\u5316\u5957\u7d44\u53ef\u514d\u9664\u4f7f\u7528\u8d85\u9ad8\u901f\u96e2\u5fc3\uff0c\u66f4\u7c21\u55ae\u3001\u5feb\u901f\u53d6\u5f97\u9ad8\u6fc3\u5ea6\u75c5\u6bd2\u6db2\u300230\u5206\u9418\u5167\u5feb\u901f\u7d14\u5316\u75c5\u6bd2\u3001\u53ef\u9054\u6fc3\u7e2e\u6548\u679c\u7d04100x (10X\u4f7f\u7528, 100ML\u5305\u88dd)<\/span>\u3002<\/p>\n

What is the mechanism of purification for the Speedy Lentivirus<\/p>\n

\u5feb\u901f\u6162\u75c5\u6bd2\u7d14\u5316\u7684\u7d14\u5316\u6a5f\u5236(mechanism)\u662f\u4ec0\u9ebc\uff1f Speedy \u6162\u75c5\u6bd2\u7d14\u5316\u662f\u4e00\u7a2e\u5e36\u6b63\u96fb\u8377\u7684\u57fa\u8cea\u6eb6\u6db2(positively charged matrix solution)\uff0c\u8207\u5e36\u8ca0\u96fb\u8377\u7684\u6162\u75c5\u6bd2\u7d50\u5408\uff0c\u5f62\u6210\u6c89\u6fb1(precipitation)\uff0c\u53ef\u900f\u904e\u96e2\u5fc3\u6c89\u6fb1\u3002<\/p>\n

<\/pre>\n
293 cells \u611f\u67d3\u7d14\u5316\u524d\uff08A\uff09\u8207\u7d14\u5316\u5f8c\uff08B\uff09\u7684lentivirus 48hr\u5f8c\u7684\u7d50\u679c<\/span><\/strong><\/p>\n
\n
\u5f9e\u57f9\u990a\u7269\u4e2d\u6536\u96c6\u6162\u75c5\u6bd2\u4e0a\u6e05\u6db2 (lentiviral supernatant)\u3002\u6536\u96c6\u7684\u4e0a\u6e05\u6db2 (supernatant) \u4ee52500 g \u96e2\u5fc310 \u5206\u9418\uff0c\u6c89\u6fb1\u7d30\u80de\u788e\u7247(cell debris); \u4e26\u901a\u904e 0.45 \u03bcm \u6ce8\u5c04\u5668\u904e\u6ffe\u5668(syringe filter)\u904e\u6ffe(filter)\u3002<\/p>\n
    \n
  1. \u5c07\u4e0a\u6b65\u904e\u6ffe\u5f8c\u7684\u75c5\u6bd2\u4e0a\u6e05\u6db2\uff0c\u6bcf 45 ml \u75c5\u6bd2\u4e0a\u6e05\u52a0\u5165 5 ml Speedy Lentivirus Purification\uff0c\u5012\u7f6e(inversion)\u6eb6\u6db2\u4e26\u5fb9\u5e95\u6df7\u5408\u3002(\u6b64\u6b65\u9a5f\u9700\u53cd\u61c9\u5b8c\u6574\uff0c\u6216\u65bc4\u5ea6C O\/N)<\/span><\/li>\n
  2. 4\u00b0C \u2265 5000g \u96e2\u5fc3 10 \u5206\u9418\u4ee5\u6536\u96c6\u75c5\u6bd2\u9846\u7c92(pellet)\u3002<\/li>\n
  3. \u5b8c\u5168\u5012\u51fa\u4e0a\u6e05\u6db2\u3002\u6b64\u6642Pellet\u70ba\u75c5\u75c5\u9846\u7c92; \u5c0f\u5fc3\u4e14\u4e0d\u8981\u7834\u58de\u75c5\u6bd2\u9846\u7c92(pellet)\u3002<\/li>\n
  4. \u52a0\u5165 0.45 ml\u00a0– 4.5 ml \u542b\u8840\u6e05\u57f9\u990a\u57fa\u4e2d\u4ee5\u5b8c\u5168\u91cd\u65b0\u61f8\u6d6e\u75c5\u6bd2\u9846\u7c92(pellet)\u3002 (\u5177\u9ad4\u53d6\u6c7a\u65bc\u75c5\u6bd2\u9700\u8981\u6fc3\u5ea6)<\/li>\n
  5. \u7acb\u5373\u5206\u88dd\u91cd\u65b0\u61f8\u6d6e\u7684\u75c5\u6bd2\u4e26\u5132\u5b58\u5728 -70\u00b0C\u3002(\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd)<\/li>\n<\/ol>\n
    293T \u7d30\u80de\u682a\u88ab\u5ee3\u6cdb\u7528\u4f5c\u6162\u75c5\u6bd2\u751f\u7522\u7684\u6700\u4f73\u7d30\u80de\u682a\u3002\u8f49\u67d3\u6642293T\u7d30\u80de\u7684\u5065\u5eb7\u72c0\u6cc1\u662f\u6162\u75c5\u6bd2\u751f\u7522\u6210\u529f\u7684\u95dc\u9375\u56e0\u7d20\u3002\u4f7f\u7528\u300c\u4e0d\u5065\u5eb7\u300d\u7d30\u80de\u6703\u5c0d\u8f49\u67d3\u6548\u7387\u7522\u751f\u8ca0\u9762\u5f71\u97ff\uff0c\u5c0e\u81f4\u6162\u75c5\u6bd2\u5eab\u5b58\u6ef4\u5ea6\u8f03\u4f4e\u3002\u70ba\u4e86\u7372\u5f97\u6700\u4f73\u6162\u75c5\u6bd2\u751f\u7522\u6548\u679c\uff0c\u8acb\u5728\u8f49\u67d3\u524d\u6309\u7167\u4ee5\u4e0b\u6307\u5357\u57f9\u990a 293T \u7d30\u80de\uff1a\u00a0 \u78ba\u4fdd\u7d30\u80de\u6d3b\u529b\u5927\u65bc 90%\u3002\u00a0 \u5728\u901a\u904e\u4e4b\u524d\u4e0d\u8981\u8b93\u7d30\u80de\u904e\u5ea6\u751f\u9577\u3002 \u4f7f\u7528\u50b3\u4ee3\u6b21\u6578\u5c11\u65bc16 \u4ee3\u7684\u7d30\u80de\u3002 \u78ba\u4fdd293T \u7d30\u80de\u6c92\u6709Mycoplasma\u6c61\u67d3\u3002<\/p>\n<\/div>\n
    Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n\n\n\n\n\n\n\n
    \u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
    LV999<\/td>\nSpeedy Lentivirus Purification<\/a><\/td>\n100ml<\/td>\n<\/tr>\n
    LV900<\/td>\nqPCR Lentivirus Titration(Titer) Kit<\/a><\/td>\n100 Reactions<\/td>\n<\/tr>\n
    LV998<\/td>\nUltra-Pure Lentivirus Purification Kit<\/a>\uff0d\u53ef\u7d14\u5316\u75c5\u6bd2\u6fc3\u5ea6\u90541011<\/sup>\u75c5\u6bd2\u9846\u7c92<\/td>\n1 Kit<\/td>\n<\/tr>\n
    G171
    \nG172
    \nG173<\/td>\n    		PuRetro\u2122 Lentivirus Purification Kit<\/a><\/td>\n2 Standard Units
    \n5 Standard Units
    \n2 Giga Units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
     <\/p>\n","protected":false},"excerpt":{"rendered":"
    Lentivirus\u6162\u75c5\u6bd2\u7d14\u5316 |\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44 \u8ca8\u865fLV999 \u91cd\u7d44\u6162\u75c5\u6bd2\u8f09\u9ad4(Recombinant  […]<\/p>\n","protected":false},"author":3,"featured_media":2474,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,57,116,456],"tags":[4570],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6146"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6146"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6146\/revisions"}],"predecessor-version":[{"id":51923,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6146\/revisions\/51923"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2474"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6146"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6146"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}