{"id":5976,"date":"2016-08-22T11:32:04","date_gmt":"2016-08-22T03:32:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=5976"},"modified":"2023-06-17T18:41:12","modified_gmt":"2023-06-17T10:41:12","slug":"ldh%e4%b9%b3%e9%85%b8%e8%84%ab%e6%b0%ab%e9%85%b6%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-ldh-cytotoxicity-colorimetric-assay-kit","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=5976","title":{"rendered":"LDH\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790 | LDH-Cytotoxicity Colorimetric Assay Kit\u8ca8\u865fK311-400 (abcam ab65391)"},"content":{"rendered":"

\u00a0<\/strong>LDH<\/strong>\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong>LDH-Cytotoxicity Colorimetric Assay Kit<\/strong><\/a>\u00a0 \u00a0(abcam ab65391)<\/span><\/p>\n

LDH\u4e73\u9178\u812b\u6c2b\u9176 (Cytotoxicity) \u5448\u8272\u5206\u6790\u5957\u7d44 \u8ca8\u865fK311-400<\/a><\/span> \u00a0| 100\u500b\u5206\u6790<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u7d93\u7531\u7d30\u80de\u819c\u7834\u640d\uff0c\u53ef\u8a55\u4f30\u7d30\u80de\u662f\u5426\u6b7b\u4ea1 (Cell death)\u6216\u7522\u751f\u7d30\u80de\u6bd2\u6027 (cytotoxicity)\u53cd\u61c9\uff1b\u7576\u7d30\u80de\u819c\u7834\u640d\u6642\uff0c\u539f\u672c\u5b58\u5728\u65bc\u7d30\u80de\u8cea\u5167\u7684\u4e73\u9178\u812b\u6c2b\u8122 (lactate dehydrogenase, LDH) \u5373\u6703\u88ab\u91cb\u653e\u81f3\u7d30\u80de\u57f9\u990a\u6db2\u4e2d\u3002\u85c9\u7531\u52a0\u5165\u53ef\u8207 LDH \u53cd\u61c9\u5f62\u6210\u6c34\u6eb6\u6027\u7d05\u8272\u7d50\u6676\u7269\u8cea\u7684\u5448\u8272\u8a66\u5291\uff0c\u5373\u53ef\u6e2c\u5f97\u7d30\u80de\u7684\u6b7b\u4ea1\u7387\u3002\u7522\u54c1\u539f\u7406: LDH\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u5076\u806f\u7684\u9176\u53cd\u61c9\u6e2c\u5b9a\uff1aLDH\u5c07\u4e73\u9178\u6c27\u5316\u6210\u4e19\u916e\u9178\uff0c\u7136\u5f8c\u5176\u8207\u56db\u5511\u9393\u9e7dINT (tetrazolium salt INT) \u53cd\u61c9\u3002\u5728\u57f9\u990a\u7269\u4e0a\u6e05\u6db2\u4e2d\u7522\u751f\u7684formazan\u7684\u91cf\u589e\u52a0\u76f4\u63a5\u8207\u88c2\u89e3\u7d30\u80de\u6578\u91cf\u7684\u589e\u52a0\u76f8\u95dc\u3002<\/p>\n

\"\"<\/p>\n

\"\u7522\u54c1\u7279\u9ede2\"<\/p>\n

LDH\u4e73\u9178\u812b\u6c2b\u9176 (Lactate Dehydrogenase, LDH) \u591a\u5b58\u65bc\u7d44\u7e54\u5668\u5b98\u5982\u809d\u81df\u3001\u5fc3\u81df\u3001\u814e\u81df\u3001\u808c\u8089\u53ca\u7d05\u8840\u7403\u4e2d\u3002\u82e5\u6578\u503c\u504f\u9ad8\uff0c\u8868\u793a\u53ef\u80fd\u60a3\u6709\u5fc3\u808c\u6897\u585e\u3001\u80ba\u6813\u585e\u3001\u809d\u81df\u640d\u50b7\u3001\u808c\u8089\u767c\u80b2\u4e0d\u826f\u3001\u767d\u8840\u75c5\u3001\u8ca7\u8840\u6216\u764c\u75c7\u7b49\u3002 Biovision\u63d0\u4f9bLDH\u5206\u6790\u5957\u7d44\uff0c\u907f\u95a7\u653e\u5c04\u7dda\u5206\u6790\u65b9\u6cd5\uff0c\u4ee5\u5448\u8272Absorbance ( 490 – 500 nm)<\/span><\/span>\u5075\u6e2c\uff1b\u53ef\u7528\u65bc\u8cbc\u9644\u578b<\/span><\/span>\u6216\u61f8\u6d6e\u578b\u7d30\u80de<\/span><\/span>\u57f9\u990a\u6db2\u3002\u64cd\u4f5c\u6642\u9593\u5927\u7d0430-60\u5206\u9418\u3002<\/p>\n

<\/p>\n

Functional studies – ab65391 LDH-Cytotoxicity Assay Kit (400 assays)<\/span><\/strong><\/div>\n
\n

LDH release measured at various cell amounts<\/p>\n<\/div>\n

HOT\u76f8\u95dc\u5546\u54c1<\/span><\/strong>|WST1<\/strong>\u7d30\u80de\u6d3b\u6027\u5206\u6790<\/strong>(<\/strong>\u5448\u8272<\/strong>)<\/strong>\u5957\u7d44<\/strong><\/a>|\u7d30\u80de\u8001\u5316\u5206\u6790\u5957\u7d44<\/strong><\/a>| \u751f\u7269\u51b7\u5149\u7d30\u80de\u6bd2\u6027\u5206\u6790\u5957\u7d44<\/strong><\/a>\u00a0| \u00a0LDH\u7d30\u80de\u6bd2\u6027\u5206\u6790\u5957\u7d44<\/a><\/strong>| \u6b7b\u6d3b\u7d30\u80de\u67d3\u8272<\/a><\/strong>|<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0K350-100<\/td>\n\u00a0Adenylate Kinase (AK) Activity Assay Kit (Colorimetric\/Fluorometric)<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K511-500<\/td>\n\u00a0Bacterial Counting Colorimetric Assay Kit\u00a0<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K511-2500<\/td>\n\u00a0Bacterial Counting Colorimetric Assay Kit\u00a0<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K315-100<\/td>\n\u00a0Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD\/CFSE)<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4230-100<\/td>\n\u00a0Chloramphenicol (CAP) ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4231-100<\/td>\n\u00a0Furazolidone ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K311-400<\/td>\n\u00a0LDH-Cytotoxicity Colorimetric Assay Kit<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a0K313-500<\/td>\n\u00a0LDH-Cytotoxicity Colorimetric Assay Kit II<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K314-500<\/td>\n\u00a0PicoProbe\u2122 LDH-Cytotoxicity Fluorometric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7d30\u80de\u6d3b\u6027<\/strong> | <\/strong>\u589e\u6b96\u76f8\u95dc\u5546\u54c1<\/strong><\/p>\n

Cytotoxicity Assays\u7d30\u80de\u6bd2\u6027\u5206\u6790<\/a> | Cell proliferation\u80de\u6d3b\u6027\u5206\u6790<\/a> | Live-Dead cell staining<\/a> | ADP & ATP Assay Kits<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0LDH\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790\u5957\u7d44LDH-Cytotoxicity Colorimetric Assay Kit\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":5979,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[123],"tags":[4597],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5976"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5976"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5976\/revisions"}],"predecessor-version":[{"id":5978,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5976\/revisions\/5978"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5979"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5976"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5976"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5976"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}