{"id":5963,"date":"2016-08-19T15:15:54","date_gmt":"2016-08-19T07:15:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=5963"},"modified":"2022-07-26T17:38:01","modified_gmt":"2022-07-26T09:38:01","slug":"calcein-am-%e6%b4%bb%e7%b4%b0%e8%83%9e%e8%9e%a2%e5%85%89%e6%9f%93%e5%8a%91-%e8%b2%a8%e8%99%9f1755-50-biovision","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=5963","title":{"rendered":"Calcein AM \u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291 | \u8ca8\u865f1755-50 Biovision"},"content":{"rendered":"

\u5546\u54c1: <\/strong>Calcein AM<\/strong> \u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/strong> | <\/strong>\u8ca8\u865f<\/strong>1755-50 |<\/strong> \u898f\u683c<\/strong>50<\/strong>ug<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> \u6d3b\u7d30\u80de\u67d3\u5291 \u201cCalcein AM\u201d, \u7528\u65bc\u6d3b\u7d30\u80de\u89c0\u5bdf\u53ca\u7d30\u80de\u4fb5\u5165\u53ca\u8f49\u79fb\u8a66\u5291\uff1b\u5206\u5b50\u578b\u5f0fC\u2084\u2086H\u2084\u2086N\u2082O\u2082\u2083 | \u5206\u5b50\u91cfMolecular Weight: 994.86 | \u7d14\u5ea6Purity: <\/strong>\u226595% by HPLC | DMSO (5 mg\/ml)\u3002Calcein-AM\u662f\u4e00\u7a2e\u53ef\u5c0d\u6d3b\u7d30\u80de\u9032\u884c\u87a2\u5149\u6a19\u8a18\u7684\u7d30\u80de\u67d3\u8272\u8a66\u5291\uff0cCalcein-AM\u7531\u65bc\u5728Calcein(\u9223\u9ec3\u7da0\u7d20)\u7684\u57fa\u790e\u4e0a\u52a0\u5f37\u4e86\u758f\u6c34\u6027\uff0c\u56e0\u6b64\u80fd\u5920\u8f15\u6613\u7a7f\u900f\u6d3b\u7d30\u80de\u819c\u3002\u7576\u5176\u9032\u5165\u5230\u7d30\u80de\u8cea\u5f8c\uff0c\u916f\u9176\u6703\u5c07\u5176\u6c34\u89e3\u70baCalcein(\u9223\u9ec3\u7da0\u7d20)\u7559\u5728\u7d30\u80de\u5167,\u767c\u51fa\u5f37\u7da0\u8272\u87a2\u5149\u3002\u8207\u5176\u4ed6\u540c\u985e\u8a66\u5291\uff08\u5982BCECF-AM\u548cCarboxy-fluorescein diacetate\uff09\u76f8\u6bd4\uff0cCalcein-AM\u662f\u6700\u9069\u5408\u4f5c\u70ba\u87a2\u5149\u63a2\u91dd\u53bb\u67d3\u6d3b\u7d30\u80de\u7684\uff0c\u56e0\u70ba\u5b83\u7684\u7d30\u80de\u6bd2\u6027\u5f88\u4f4e\u3002Calcein(\u9223\u9ec3\u7da0\u7d20)\u4e0d\u6703\u6291\u5236\u4efb\u4f55\u7684\u7d30\u80de\u529f\u80fd\u5982\u589e\u6b96\u6216\u6dcb\u5df4\u7403\u7684\u8da8\u5316\u6027\u3002\u53e6\u5916\uff0c\u4f7f\u7528\u4e86Calcein(\u9223\u9ec3\u7da0\u7d20)\u7684\u6d3b\u6027\u6aa2\u6e2c\u7684\u5be6\u9a57\u7d50\u679c\u662f\u5341\u5206\u53ef\u4fe1\u4e26\u4e14\u8207\u6a19\u6e96\u768451<\/sup>Cr-\u91cb\u653e\u6cd5\u6240\u5f97\u7d50\u679c\u76f8\u4e00\u81f4\u7684\u3002\u82e5\u9700\u5957\u7d44\u5305\u88dd\u8acb\u8a02\u8cfcK305\u00a0Calcein AM<\/strong>\u7d30\u80de\u6d3b\u529b\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong><\/a><\/p>\n

\"\"<\/p>\n

Calcein AM<\/strong> \u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/strong> | <\/strong>\u7528\u65bc\u7d30\u80de\u6d3b\u6027\u5206\u6790\u4e4b\u5efa\u8b70<\/strong>\u914d\u88fd<\/strong><\/p>\n

Calcein AM\u5206\u5b50\u91cf\u70ba995 g\/mole , \u7a31\u53d650ug\u7684calcein AM\u53ef\u4ee5\u6eb6\u572850ul\u7684DMSO (1755-5<\/a><\/span>\u70ba50ug\u5305\u88dd; 1755-250<\/a><\/span>\u70ba5x50ug\u5305\u88dd)\uff1b\u6700\u5f8c\u6fc3\u5ea6\u70ba1Mm\u53d61ul\u7684Calcein AM \u6eb6\u57281X \u76841000ul dissociation solution =1uM (1000ul)\u4ee596well \u70ba\u4f8b\uff0c\u52a0\u5165100ul\u76841uM Calcein AM,\u5927\u7d04\u53ef\u4ee5\u4f7f\u7528500\u6b21\u3002Calcein-AM\u7684ester\u90e8\u4f4d\u9047\u5230\u6fd5\u6c23\u6703\u5206\u89e3\uff0c\u4f7f\u7528\u5f8c\u8acb\u5728-20\u2103\u4e0b\u5bc6\u9589\u4fdd\u5b58\uff0c\u6eb6\u65bcDMSO\u5f8c\u5efa\u8b702\u500b\u6708\u5167\u4f7f\u7528\u5b8c\u3002\u8a66\u5291\u5957\u7d44\u5305\u88dd\u8acb\u6309\u6b64\u00a0Calcein AM<\/strong>\u7d30\u80de\u6d3b\u529b\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong><\/a><\/p>\n

Note: The final concentration of the Calcein AM will need to be empirically determined for different cell types and\/or experimental conditions. Ranges of 1 – 10 M have been reported.<\/p>\n

Calcein AM<\/strong> \u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/strong> | <\/strong>\u6b7b\u6d3b\u7d30\u80de\u96d9\u91cd\u67d3\u8272<\/strong><\/p>\n

Calcein(\u9223\u9ec3\u7da0\u7d20)\u7684\u6fc0\u767c\u548c\u767c\u5c04\u6ce2\u9577\u5206\u5225\u70ba490 nm\u548c515 nm\u3002Calcein-AM\u548c\u7898\u5316\u4e19\u5576\uff08PI\uff09\u6eb6\u6db2\uff0c\u5b83\u5011\u5206\u5225\u53ef\u5c0d\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u67d3\u8272\u3002Calcein-AM\u7684\u4e59\u9178\u7532\u57fa\u916f\u89aa\u8102\u6027\u5f88\u9ad8\uff0c\u4f7f\u5176\u53ef\u900f\u904e\u7d30\u80de\u819c\uff0c\u901a\u904e\u6d3b\u7d30\u80de\u5167\u7684\u916f\u9176\u4f5c\u7528\uff0cCalcein-AM\u80fd\u812b\u53bbAM\u57fa\uff0c\u7522\u751f\u7684Calcein(\u9223\u9ec3\u7da0\u7d20)\u80fd\u767c\u51fa\u5f37\u7da0\u8272\u87a2\u5149\uff0c\u56e0\u6b64Calcein-AM\u50c5\u5c0d\u6d3b\u7d30\u80de\u67d3\u8272\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u4f5c\u70ba\u6838\u67d3\u8272\u67d3\u6599\u7684PI\u4e0d\u80fd\u7a7f\u904e\u6d3b\u7d30\u80de\u7684\u7d30\u80de\u819c\u3002\u5b83\u7a7f\u904e\u6b7b\u7d30\u80de\u819c\u7684\u7121\u5e8f\u5340\u57df\u800c\u5230\u9054\u7d30\u80de\u6838\uff0c\u4e26\u5d4c\u5165\u7d30\u80de\u7684DNA\u96d9\u87ba\u65cb\u5f9e\u800c\u7522\u751f\u7d05\u8272\u87a2\u5149\uff08\u6fc0\u767c\uff1a535 nm\uff0c\u767c\u5c04\uff1a617 nm\uff09\uff0c\u56e0\u6b64PI\u50c5\u5c0d\u6b7b\u7d30\u80de\u67d3\u8272\u3002\u7531\u65bcCalcein(\u9223\u9ec3\u7da0\u7d20)\u548cPI-DNA\u90fd\u53ef\u88ab490 nm\u6fc0\u767c\uff0c\u56e0\u6b64\u53ef\u7528\u87a2\u5149\u986f\u5fae\u93e1\u540c\u6642\u89c0\u5bdf\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u3002\u7528545 nm\u6fc0\u767c\uff0c\u50c5\u53ef\u89c0\u5bdf\u5230\u6b7b\u7d30\u80de\u3002\u6839\u64da\u4ee5\u4e0a\u7279\u9ede\uff0cCalcein-AM\u548c\u7898\u5316\u4e19\u5576\uff08PI\uff09\u7d93\u5e38\u88ab\u7d50\u5408\u7528\u4f86\u4f5c\u70ba\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u7684\u96d9\u91cd\u67d3\u8272\u3002<\/p>\n

BIOVISION and TREVIGEN\u2122 Calcein AM Fluorescent Dye can be used to fluorescently label viable cells for a variety of assays such as tumor cell invasion, endothelial cell migration, and endothelial cell tubulogenesis.<\/p>\n

Calcein AM<\/strong> \u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/strong> | <\/strong>\u76f8\u95dc\u5546\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n
\u00a0Catalog#<\/a><\/td>\n\u00a0Product Name<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a01755-1000<\/td>\n\u00a0Calcein AM\u00a0<\/a><\/td>\n\u00a01 mg<\/td>\n<\/tr>\n
\u00a01755-50<\/td>\n\u00a0Calcein AM<\/a><\/td>\n\u00a050 \u00b5g<\/td>\n<\/tr>\n
\u00a01755-250<\/td>\n\u00a0Calcein AM<\/a><\/td>\n\u00a05 x 50 \u00b5g<\/td>\n<\/tr>\n
\u00a0K305-1000<\/td>\n\u00a0EZViable\u2122 Calcein AM Cell Viability Assay Kit (Fluorometric)<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u672a\u547d\u540d-14\"\u00a0<\/b>|<\/b><\/span>\u66f4\u591a<\/strong>P<\/strong>robe<\/strong>\u76f8\u95dc\u5546\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a02752-100<\/td>\n\u00a0EZSolution\u2122 DAF-2<\/a><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a02752-500<\/td>\n\u00a0EZSolution\u2122 DAF-2<\/a><\/td>\n\u00a0500 \u00b5g<\/td>\n<\/tr>\n
\u00a02752-1000<\/td>\n\u00a0EZSolution\u2122 DAF-2<\/a><\/td>\n\u00a01 mg<\/td>\n<\/tr>\n
\u00a02753-100<\/td>\n\u00a0EZSolution\u2122 DAF-2 diacetate<\/a><\/td>\n\u00a0100 \u00b5g<\/td>\n<\/tr>\n
\u00a02753-500<\/td>\n\u00a0EZSolution\u2122 DAF-2 diacetate<\/a><\/td>\n\u00a0500 \u00b5g<\/td>\n<\/tr>\n
\u00a02753-1000<\/td>\n\u00a0EZSolution\u2122 DAF-2 diacetate<\/a><\/td>\n\u00a01 mg<\/td>\n<\/tr>\n
2760-1<\/td>\n\u00a02-NBDG<\/a><\/td>\n\u00a01 mg<\/td>\n<\/tr>\n
\u00a02760-5<\/td>\n\u00a02-NBDG<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a09565-10<\/td>\n\u00a05-Ethynyl-2′-deoxyuridine<\/a><\/td>\n\u00a010 mg<\/td>\n<\/tr>\n
\u00a09565-50<\/td>\n\u00a05-Ethynyl-2′-deoxyuridine<\/a><\/td>\n\u00a050 mg<\/td>\n<\/tr>\n
\u00a02810-1G<\/td>\n\u00a0BCA disodium salt<\/a><\/td>\n\u00a01 g<\/td>\n<\/tr>\n
\u00a02810-5G<\/td>\n\u00a0BCA disodium salt<\/a><\/td>\n\u00a05 g<\/td>\n<\/tr>\n
\u00a02810-100G<\/td>\n\u00a0BCA disodium salt<\/a><\/td>\n\u00a0100 g<\/td>\n<\/tr>\n
\u00a02810-500G<\/td>\n\u00a0BCA disodium salt<\/a><\/td>\n\u00a0500 g<\/td>\n<\/tr>\n
\u00a02594-1<\/td>\n\u00a0BODIPY-Acetylene Reagent<\/a><\/td>\n\u00a01 mg<\/td>\n<\/tr>\n
\u00a02594-5<\/td>\n\u00a0BODIPY-Acetylene Reagent<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a0B1029-5<\/td>\n\u00a0Coumarin Boronic acid\u00a0<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a0B1029-25<\/td>\n\u00a0Coumarin Boronic acid\u00a0<\/a><\/td>\n\u00a025 mg<\/td>\n<\/tr>\n
\u00a02699-5<\/td>\n\u00a0CRANAD-2<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a02699-25<\/td>\n\u00a0CRANAD-2<\/a><\/td>\n\u00a025 mg<\/td>\n<\/tr>\n
\u00a02744-500<\/td>\n\u00a0Filipin III<\/a><\/td>\n\u00a0500 \u00b5g<\/td>\n<\/tr>\n
\u00a02744-1000<\/td>\n\u00a0Filipin III<\/a><\/td>\n\u00a01 mg<\/td>\n<\/tr>\n
\u00a09695-5<\/td>\n\u00a0Fluorescent sensor, ANQ\u00a0<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a09695-25<\/td>\n\u00a0Fluorescent sensor, ANQ\u00a0<\/a><\/td>\n\u00a025 mg<\/td>\n<\/tr>\n
\u00a01689-05<\/td>\n\u00a0Jasplakinolide, jaspis johnstoni<\/a><\/td>\n\u00a050 \u00b5g<\/td>\n<\/tr>\n
\u00a01130-5<\/td>\n\u00a0JC-1<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a02664-5<\/td>\n\u00a0Methoxy-X04<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a02664-25<\/td>\n\u00a0Methoxy-X04<\/a><\/td>\n\u00a025 mg<\/td>\n<\/tr>\n
\u00a02808-250<\/td>\n\u00a0MTS Reagent <\/a><\/td>\n\u00a0250 mg<\/td>\n<\/tr>\n
\u00a02808-1000<\/td>\n\u00a0MTS Reagent <\/a><\/td>\n\u00a01 g<\/td>\n<\/tr>\n
\u00a02809-1G<\/td>\n\u00a0MTT Reagent<\/a><\/td>\n\u00a01 g<\/td>\n<\/tr>\n
\u00a02809-5G<\/td>\n\u00a0MTT Reagent<\/a><\/td>\n\u00a05 x 1 g<\/td>\n<\/tr>\n
\u00a02710-5<\/td>\n\u00a0NIAD-4<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a02710-25<\/td>\n\u00a0NIAD-4<\/a><\/td>\n\u00a025 mg<\/td>\n<\/tr>\n
\u00a01572-5<\/td>\n\u00a0OxiRed\u2122 Probe<\/a><\/td>\n\u00a05 mg<\/td>\n<\/tr>\n
\u00a01572-10-5<\/td>\n\u00a0OxiRed\u2122 Probe<\/a><\/td>\n\u00a010×5 mg<\/td>\n<\/tr>\n
\u00a01572-20-5<\/td>\n\u00a0OxiRed\u2122 Probe<\/a><\/td>\n\u00a020×5 mg<\/td>\n<\/tr>\n
\u00a02198-10<\/td>\n\u00a0WST-1<\/a><\/td>\n\u00a010 mg<\/td>\n<\/tr>\n
\u00a02198-30<\/td>\n\u00a0WST-1<\/a><\/td>\n\u00a030 mg<\/td>\n<\/tr>\n
\u00a02814-100<\/td>\n\u00a0WST-3<\/a><\/td>\n\u00a0100 mg<\/td>\n<\/tr>\n
\u00a02814-500<\/td>\n\u00a0WST-3<\/a><\/td>\n\u00a0500 mg<\/td>\n<\/tr>\n
\u00a02814-1000<\/td>\n\u00a0WST-3<\/a><\/td>\n\u00a01 g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u672a\u547d\u540d-14\"\u00a0<\/b>|<\/b><\/span> Nitric Oxide (NO) Probes<\/strong><\/a> | Other Probes<\/strong><\/a> | Zinc Probes<\/strong><\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: Calcein AM \u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291 | \u8ca8\u865f1755-50 | \u898f\u683c50ug \u7522\u54c1\u4ecb\u7d39: \u6d3b\u7d30\u80de\u67d3\u5291 […]<\/p>\n","protected":false},"author":3,"featured_media":5627,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[180],"tags":[4599],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5963"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5963"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5963\/revisions"}],"predecessor-version":[{"id":18460,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5963\/revisions\/18460"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5627"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5963"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5963"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}