{"id":54780,"date":"2026-04-25T13:21:14","date_gmt":"2026-04-25T05:21:14","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=54780"},"modified":"2026-04-25T13:21:29","modified_gmt":"2026-04-25T05:21:29","slug":"vegfa%e8%a1%80%e7%ae%a1%e5%85%a7%e7%9a%ae%e7%94%9f%e9%95%b7%e5%9b%a0%e5%ad%90a-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-uscn%e8%b2%a8%e8%99%9fusea143hu","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=54780","title":{"rendered":"VEGFA\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50A ELISA\u8a66\u5291\u76d2 | USCN\u8ca8\u865fUSEA143Hu"},"content":{"rendered":"

VEGFA\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50A ELISA\u8a66\u5291\u76d2 | ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) USCN\u8ca8\u865fUSEA143Hu<\/span><\/span><\/a><\/p>\n

ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)<\/span><\/p>\n

\u5546\u54c1:<\/strong> \u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50A (Vascular Endothelial Growth Factor A, VEGFA)<\/span> ELISA\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) Double-antibody Sandwich \u8ca8\u865fUSEA143Hu\uff0c\u61c9\u7528\u7269\u7a2eHomo sapiens (Human)\u00a0 \uff1b\u5075\u6e2c\u7bc4\u570d15.6-1,000pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6 6.2pg\/mL\u3002\u53ef\u61c9\u7528\u65bcserum, plasma and other biological fluids\u751f\u7269\u6a23\u54c1\u4e4b\u6aa2\u6e2c\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002USCN\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/strong><\/a><\/span><\/p>\n

<\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

VEGFA\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50A ELISA\u5957\u7d44\u5167\u5bb9<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Pre-coated, ready to use 96-well strip plate<\/td>\n1<\/td>\nPlate sealer for 96 wells<\/td>\n4<\/td>\n<\/tr>\n
Standard<\/td>\n2<\/td>\nStandard Diluent<\/td>\n1\u00d720mL<\/td>\n<\/tr>\n
Detection Reagent A<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent A<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
Detection Reagent B<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent B<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n1\u00d79mL<\/td>\nStop Solution<\/td>\n1\u00d76mL<\/td>\n<\/tr>\n
Wash Buffer (30 \u00d7 concentrate)<\/td>\n1\u00d720mL<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

VEGFA\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50A\u76f8\u95dc\u4e4b\u6297\u9ad4 | \u86cb\u767d\u8cea | ELISA | CLIA\u7522\u54c1<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nOrganism species: Homo sapiens (Human)<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
URPA143Hu02<\/td>\nRecombinant Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
URPA143Hu01<\/td>\nRecombinant Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
UEPA143Hu61<\/td>\nEukaryotic Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
UAPA143Hu01<\/td>\nActive Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
UPAA143Hu01<\/td>\nPolyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nIHC<\/td>\n<\/tr>\n
UPAA143Hu02<\/td>\nPolyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB; IHC<\/td>\n<\/tr>\n
ULAA143Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nIHC<\/td>\n<\/tr>\n
ULAA143Hu81<\/td>\nFITC-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB; IHC; ICC; IF.<\/td>\n<\/tr>\n
URAA143Hu21<\/td>\nRecombinant Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB; IF; ICC; IHC; IP; FCM.<\/td>\n<\/tr>\n
UMAA143Hu24<\/td>\nMonoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UMAA143Hu26<\/td>\nMonoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UMAA143Hu21<\/td>\nMonoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UFAA143Hu02<\/td>\nAnti-Vascular Endothelial Growth Factor A (VEGFA) Monoclonal Antibody<\/a><\/td>\nFCM<\/td>\n<\/tr>\n
ULAA143Hu76<\/td>\nBiotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
ULMA143Hu<\/td>\nMultiplex Assay Kit for Vascular Endothelial Growth Factor A (VEGFA) ,etc. by FLIA (Flow Luminescence Immunoassay)<\/a><\/td>\nFLIA Kit for Antigen Detection.<\/td>\n<\/tr>\n
UHEA143Hu<\/td>\nHigh Sensitive ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
USEA143Hu<\/td>\nELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
UMEA143Hu<\/td>\nMini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
USCA143Hu<\/td>\nCLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n
UPSA143Hu01<\/td>\nAntibody Pair for Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.<\/td>\n<\/tr>\n
UKSA143Hu01<\/td>\nELISA Kit DIY Materials for Vascular Endothelial Growth Factor A (VEGFA)<\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Assay procedure summary of the ELISA Kit for VEGFA\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50A<\/span><\/strong><\/h3>\n
\n

1. Prepare all reagents, samples and standards;
\n2. Add 100\u00b5L standard or sample to each well. Incubate 1 hours at 37\u00b0C;
\n3. Aspirate and add 100\u00b5L prepared Detection Reagent A. Incubate 1 hour at 37\u00b0C;
\n4. Aspirate and wash 3 times;
\n5. Add 100\u00b5L prepared Detection Reagent B. Incubate 30 minutes at 37\u00b0C;
\n6. Aspirate and wash 5 times;
\n7. Add 90\u00b5L Substrate Solution. Incubate 10-20 minutes at 37\u00b0C;
\n8. Add 50\u00b5L Stop Solution. Read at 450nm immediately.<\/p>\n<\/div>\n

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\"\"<\/span><\/strong>\u4e09\u660e\u6cbbELISA<\/span><\/span><\/h3>\n<\/div>\n