{"id":54515,"date":"2026-03-24T19:24:44","date_gmt":"2026-03-24T11:24:44","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=54515"},"modified":"2026-03-24T19:25:10","modified_gmt":"2026-03-24T11:25:10","slug":"%e8%85%a6%e6%ba%90%e6%80%a7%e7%a5%9e%e7%b6%93%e7%87%9f%e9%a4%8a%e5%9b%a0%e5%ad%90%ef%bc%88bdnf%ef%bc%89elisa%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fusea011ra","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=54515","title":{"rendered":"\u8166\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff08BDNF\uff09ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2| \u8ca8\u865fUSEA011Ra"},"content":{"rendered":"

\u8166\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff08BDNF\uff09ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2| \u8ca8\u865fUSEA011Ra<\/span><\/span><\/span><\/a><\/p>\n

ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) \u5075\u6e2c\u9748\u654f\u5ea631.2-2,000pg\/mL<\/span><\/p>\n

BDNF\u662f\u4e00\u7a2e13kDa\uff0c119\u500b\u6c28\u57fa\u9178\uff08aa\uff09\u6b98\u57fa\u7684\u975e\u7cd6\u57fa\u5316\u591a\u80bd\uff0c\u5176\u4e3b\u8981\u7d50\u69cb\u5728\u6240\u6aa2\u6e2c\u7684\u6240\u6709\u54fa\u4e73\u52d5\u7269\u4e2d\u4fdd\u5b88\u3002 \u6700\u521d\u4f5c\u70ba247aa\u6b98\u57fa\u524d\u80bd\u5408\u6210\uff0c\u5c07BDNF\u5206\u5b50\u5206\u70ba18\u500b\u6c28\u57fa\u9178\u6b98\u57fa\u4fe1\u865f\u5e8f\u5217\uff0c110\u500b\u6c28\u57fa\u9178\u6b98\u57fa\u548c119\u500b\u6c28\u57fa\u9178\u6b98\u57fa\u6210\u719f\u7247\u6bb5\u3002\u8207\u5176\u4ed6\u795e\u7d93\u71df\u990a\u56e0\u5b50\u985e\u4f3c\uff0c\u5b58\u5728N-\u672b\u7aef\u53ef\u9078\u5730\u526a\u63a5\u7684\u53ef\u80fd\u6027\uff0c\u7522\u751f\u5177\u6709\u4e0d\u540c\u529f\u80fd\u7279\u6027\u7684\u66f4\u9577\u7684\u524d\u671f\uff08\u4f46\u76f8\u540c\u7684\u6210\u719f\u5340\u6bb5\uff09\u3002 \u4f5c\u70ba\u6210\u719f\u5206\u5b50\uff0cBDNF\u5728\u6c28\u57fa\u9178\u6c34\u5e73\u4e0a\u8207NGF\u76f8\u540c52\uff05\uff0c\u4f5c\u70ba\u975e\u5171\u50f9\u9023\u63a5\u7684\u540c\u578b\u4e8c\u805a\u9ad4\u5728\u6eb6\u6db2\u4e2d\u5b58\u5728\uff0c\u4e26\u4e14\u542b\u6709\u88ab\u8a8d\u70ba\u5f62\u6210\u4e09\u500b\u93c8\u5167\u4e8c\u786b\u9375\u7684\u516d\u500b\u534a\u80f1\u6c28\u9178\u6b98\u57fa\u3002<\/p>\n

\u5546\u54c1:<\/strong> \u8166\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff08BDNF\uff09ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/strong>(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/u>| Double-antibody Sandwich\u8ca8\u865fUSEA011Ra\uff0c\u61c9\u7528\u7269\u7a2eRattus norvegicus (Rat)\uff1b\u61c9\u7528\u7269\u7a2eRattus norvegicus (Rat)\uff1b\u5075\u6e2c\u7bc4\u570d31.2-2,000pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea613.3pg\/mL. \u53ef\u61c9\u7528\u65bcserum, plasma, tissue homogenates, cell lysates, cell culture supernates\u53ca\u5404\u7a2e\u9ad4\u6db2\u4e4b\u6a23\u54c1\u6aa2\u6e2c\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002USCN\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002<\/p>\n

<\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\u8166\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff08BDNF\uff09ELISA\u8a66\u5291\u76d2\u5957\u7d44\u5167\u5bb9<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Pre-coated, ready to use 96-well strip plate<\/td>\n1<\/td>\nPlate sealer for 96 wells<\/td>\n4<\/td>\n<\/tr>\n
Standard<\/td>\n2<\/td>\nStandard Diluent<\/td>\n1\u00d720mL<\/td>\n<\/tr>\n
Detection Reagent A<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent A<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
Detection Reagent B<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent B<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n1\u00d79mL<\/td>\nStop Solution<\/td>\n1\u00d76mL<\/td>\n<\/tr>\n
Wash Buffer (30 \u00d7 concentrate)<\/td>\n1\u00d720mL<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Assay procedure summary of the ELISA Kit for \u8166\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff08BDNF\uff09 <\/span><\/strong><\/h3>\n
\n

1. Prepare all reagents, samples and standards;
\n2. Add 100\u00b5L standard or sample to each well. Incubate 1 hours at 37\u00b0C;
\n3. Aspirate and add 100\u00b5L prepared Detection Reagent A. Incubate 1 hour at 37\u00b0C;
\n4. Aspirate and wash 3 times;
\n5. Add 100\u00b5L prepared Detection Reagent B. Incubate 30 minutes at 37\u00b0C;
\n6. Aspirate and wash 5 times;
\n7. Add 90\u00b5L Substrate Solution. Incubate 10-20 minutes at 37\u00b0C;
\n8. Add 50\u00b5L Stop Solution. Read at 450nm immediately.<\/p>\n<\/div>\n

\u8166\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff08BDNF\uff09ELISA\u8a66\u5291\u76d2 \u76f8\u95dc\u4e4b\u6297\u9ad4 | \u86cb\u767d\u8cea | ELISA | CLIA\u7522\u54c1<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nOrganism species: Rattus norvegicus (Rat)<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
UAPA011Ra01<\/td>\nActive Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
URPA011Ra01<\/td>\nRecombinant Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
UPAA011Ra01<\/td>\nPolyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UFAA011Ra01<\/td>\nAnti-Brain Derived Neurotrophic Factor (BDNF) Polyclonal Antibody<\/a><\/td>\nFCM<\/td>\n<\/tr>\n
UMAA011Ra25<\/td>\nMonoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UMAA011Ra22<\/td>\nMonoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UMAA011Ra23<\/td>\nMonoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UMAA011Ra24<\/td>\nMonoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
UMAA011Ra27<\/td>\nMonoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nICC\/IF<\/td>\n<\/tr>\n
UMAA011Ra21<\/td>\nMonoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nTesting in progress<\/td>\n<\/tr>\n
USEA011Ra<\/td>\nELISA Kit for Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
ULMA011Ra<\/td>\nMultiplex Assay Kit for Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay)<\/a><\/td>\nFLIA Kit for Antigen Detection.<\/td>\n<\/tr>\n
UKSA011Ra01<\/td>\nELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF)<\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n

\"\"<\/span><\/strong>\u4e09\u660e\u6cbbELISA<\/span><\/span><\/h3>\n<\/div>\n