{"id":5431,"date":"2016-08-04T17:01:05","date_gmt":"2016-08-04T09:01:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=5431"},"modified":"2023-10-24T11:16:16","modified_gmt":"2023-10-24T03:16:16","slug":"human-igg1-kappa%e7%b4%94%e5%8c%96%e4%b9%8b%e5%85%8d%e7%96%ab%e7%90%83%e8%9b%8b%e7%99%bd%e7%94%a2%e5%93%81-isotype-control","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=5431","title":{"rendered":"Human IgG1 Kappa | \u7d14\u5316\u4e4b\u514d\u75ab\u7403\u86cb\u767d\u7522\u54c1 Isotype control\u8ca8\u865f0151K-01"},"content":{"rendered":"
\u540c\u578b\u5c0d\u7167(Isotype control)-Human IgG1 Kappa<\/span><\/span><\/strong><\/span><\/pre>\n
\u540c\u578b\u5c0d\u7167(Isotype control)\u76ee\u7684<\/strong>\u78ba\u5b9a\u4e00\u6297\u7684\u7d50\u5408\u662f\u5426\u7279\u7570\u6027\uff1b\u800c\u4e0d\u662f\u975e\u7279\u7570\u6027\u7684Fc\u53d7\u9ad4\u6216\u8207\u5176\u4ed6\u86cb\u767d\u7684\u76f8\u4e92\u4f5c\u7528\u6240\u9020\u6210\u7684Signal\u3002\u9084\u53ef\u4ee5\u7528\u4f86\u7af6\u722d\u6027\u7684\u7d50\u5408\u6297\u9ad4\uff0c\u8207\u529f\u80fd\u963b\u65b7\u6297\u9ad4\u767c\u63ee\u540c\u6a23\u7684\u529f\u80fd\u3002\u540c\u578b\u5c0d\u7167(Isotype control)\u61c9\u7528\u65bc\u6d41\u6c0f\u7d30\u80de\u5100\u6297\u9ad4\u5206\u578bcontrol\u3001ELISA\u6297\u9ad4\u5206\u578bcontrol\u3001FLISA\u6297\u9ad4\u5206\u578bcontrol \u3001IHC\u6297\u9ad4\u5206\u578bcontrol \u3001IP\u6297\u9ad4\u5206\u578bcontrol \u3001invitro \u6297\u9ad4\u5206\u578bcontrol \u3001invivo\u6297\u9ad4\u5206\u578bcontrol\u3002\u4f7f\u7528Isotype control\u7684\u539f\u5247: \u540c\u578b\u5c0d\u7167\/\u5206\u578b\u5c0d\u7167\u8981\u8207\u4e00\u6297\u7684\u4f86\u6e90\uff0cIg\u5206\u578b\u548c\u6a19\u8a18\u5b8c\u5168\u4e00\u81f4\u3002\u540c\u578b\u5c0d\u7167\/\u5206\u578b\u5c0d\u7167\uff08Isotype Control\uff09\uff1a\u4f7f\u7528\u8207\u4e00\u6297\u76f8\u540c\u7a2e\u5c6c\u4f86\u6e90\u3001\u76f8\u540c\u4e9e\u578b\u3001\u76f8\u540c\u5291\u91cf\u548c\u76f8\u540c\u7684\u514d\u75ab\u7403\u86cb\u767d\u53ca\u4e9e\u578b\u7684\u514d\u75ab\u7403\u86cb\u767d\uff0c\u7528\u65bc\u6d88\u9664\u7531\u65bc\u6297\u9ad4\u975e\u7279\u7570\u6027\u7d50\u5408\u5230\u7d30\u80de\u8868\u9762\u800c\u7522\u751f\u7684\u80cc\u666f\u67d3\u8272\u3002\u5982\u679c\u4e00\u6297\u662f\u591a\u6297\uff0c\u53ef\u4ee5\u7528an normal serum\uff08\u8207\u4e00\u6297\u76f8\u540c\u7684\u6b63\u5e38\u8840\u6e05\uff09\uff0c\u6b64\u65b9\u6cd5\u5e38\u5728IHC\u67d3\u8272\u4e2d\u5e38\u88ab\u7528\u65bc\u8ca0\u5c0d\u7167\u7d44\uff1b\u5047\u82e5\u591a\u91cd\u87a2\u5149\u67d3\u8272\uff0c\u7531\u65bc\u87a2\u5149\u6a19\u8a18\u55ae\u6297\u7684\u4f86\u6e90\u4e0d\u540c\uff0c\u61c9\u9078\u7528\u76f8\u540c\u4f86\u6e90\u7684\u672a\u6a19\u8a18\u55ae\u6297\u4f5c\u70ba\u540c\u578b\u5c0d\u7167\u4f86 \u8abf\u6574\u80cc\u666f\u67d3\u8272\u3002\u4f8b\u5b50\u8aaa\u660e: \u6bd4\u5982\u6aa2\u6e2c\u4e00\u6297\u70ba\u55ae\u6297\u7684mouse anti rat CD11b\uff0cclone OX-42 purified IgG\uff0c\u90a3\u9ebc\u5b83\u7684isotype \u662fmouse IgG2a\uff0c \u6240\u4ee5\u53ef\u4ee5\u7528purified\uff08\u7d14\u5316\u7684\uff09 mouse IgG2a\u4f86\u505aOX-42\u7684\u540c\u578b\u5c0d\u7167\uff08Isotype Control\uff09\u3002<\/p>\n
Human IgG1<\/sub> Kappa <\/span><\/strong>Isotype control-<\/strong>\u7d14\u5316\u4e4b\u514d\u75ab\u7403\u86cb\u767d\u7522\u54c1\u00a0<\/strong>\u8ca8\u865f<\/strong>0151k (human) | <\/strong>\u898f\u683c<\/strong>0.5mg \/1ml<\/strong><\/span><\/a><\/span><\/p>\n
\"SB\"<\/a><\/p>\n
\u5206\u578b\u5c0d\u7167\u7d44Human IgG1<\/sub> Kappa Isotype control | \u7522\u54c1\u61c9\u7528\u6d41\u6c0f\u7d30\u80de\u5100(flow cytometry)\u6297\u9ad4\u5206\u578bcontrol\u3001ELISA\u6297\u9ad4\u5206\u578bcontrol\u3001FLISA\u6297\u9ad4\u5206\u578bcontrol \u3001IHC\u6297\u9ad4\u5206\u578bcontrol \u3001IP(immunoprecipitation)\u6297\u9ad4\u5206\u578bcontrol \u3001invitro \u6297\u9ad4\u5206\u578bcontrol \u3001invivo\u6297\u9ad4\u5206\u578bcontrol\u3001Fc\u4ecb\u5c0e\u7684\u523a\u6fc0(Fc mediated stimulation)\u3001\u8868\u9762\u7b49\u96e2\u5b50\u9ad4\u5171\u632f\u4e2d\u7684\u56fa\u5b9a\u5316( immobilization in surface plasmon resonance)\u3002<\/p>\n
<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Isotype<\/th>\nHuman IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Source<\/th>\nHuman myeloma serum<\/td>\n<\/tr>\n
Conjugate<\/th>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/th>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Clonality<\/th>\nPurified from myeloma serum<\/td>\n<\/tr>\n
Concentration<\/th>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/th>\n1.0 mL<\/td>\n<\/tr>\n
Recommended Storage<\/th>\n2-8\u00b0C<\/td>\n<\/tr>\n
Applications<\/th>\nQuality tested applications for relevant formats include –
\nELISA\u00a01,2<\/sup>
\nFLISAOther referenced applications for relevant formats include –
\nFlow Cytometry\u00a08,9<\/sup>
\nImmunoprecipitation\u00a03<\/sup>
\nIn vitro<\/i>\u00a0control\u00a04-6<\/sup>
\nIn vivo<\/i>\u00a0control\u00a01,7<\/sup>
\nStimulation (Fc mediated)\u00a010<\/sup>
\nBlocking\u00a06<\/sup>
\nSurface Plasmon Resonance\u00a01<\/sup><\/td>\n<\/tr>\n
RRID Number<\/th>\nAB_2794079<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n
\"\"<\/p>\n
\u7d30\u80de\u514d\u75ab\u5c08\u5bb6\u6975\u529b\u63a8\u85a6SouthernBiotech\u9ad8\u54c1\u8ceaIsotype control<\/strong><\/span><\/p>\n
\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8ceaIsotype control-\u514d\u75ab\u7403\u86cb\u767d\u7cfb\u5217\u7522\u54c1\uff0c\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002\u00a0<\/strong><\/p>\n
H<\/strong>uman<\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong> | <\/strong>\u63d0\u4f9b\u7d14\u5316\u4e4b<\/strong>IgG1<\/sub> Kappa<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
\u00a0Cat. No.<\/td>\nProduct Description<\/td>\nClone<\/td>\nSize<\/td>\n<\/tr>\n
0151K-01 <\/a><\/td>\nHuman IgG1<\/sub> Kappa-UNLB<\/td>\n<\/td>\n0.5 mg<\/td>\n<\/tr>\n
0151K-04 <\/a><\/td>\nHuman IgG1<\/sub> Kappa-AP<\/td>\n<\/td>\n1.0 mL<\/td>\n<\/tr>\n
0151K-05 <\/a><\/td>\nHuman IgG1<\/sub> Kappa-HRP<\/td>\n<\/td>\n1.0 mL<\/td>\n<\/tr>\n
0151K-09 <\/a><\/td>\nHuman IgG1<\/sub> Kappa-PE<\/td>\n<\/td>\n0.1 mg<\/td>\n<\/tr>\n
0151K-14 <\/a><\/td>\nHuman IgG1<\/sub> Kappa-LE\/AF<\/td>\n<\/td>\n0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\u5176\u5b83\u7269\u7a2e\u4e4b\u5206\u578b\u5c0d\u7167\u7d44 | \u63d0\u4f9b\u7d14\u5316\u53ca\u4e0d\u540c\u5206\u578b\u4e4bIsotype control<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Isotype Controls<\/strong><\/td>\n<\/tr>\n
\n
    \n
  • Canine IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Mouse IgG1 Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Chicken IgY Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Mouse IgG2a Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Donkey IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Mouse IgG2b Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Goat F(ab\u2019)2 IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Mouse IgG2c Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Goat IgG Fab Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Mouse IgG3 Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Goat IgG Fc Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Mouse IgM Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Goat IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rabbit F(ab\u2019)2 IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Hamster IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rabbit IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgA Kappa Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rabbit IgM Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgA Lambda Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgG Fc Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgG1 Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgG2a Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgG1 Kappa Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgG2b Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgG1 Lambda Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgG2b Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgG3 Lambda Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgG2c Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Human IgM Lambda Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rat IgM Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Mouse F(ab\u2019)2 IgG1 Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Rhesus Monkey IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Mouse IgA Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Sheep IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Mouse IgE Isotype Control<\/li>\n<\/ul>\n<\/td>\n
\n
    \n
  • Swine IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
\n
    \n
  • Mouse IgG Isotype Control<\/li>\n<\/ul>\n<\/td>\n
<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\u5f15\u7528isotype control \u4e4b\u6587\u737b\u53c3\u8003<\/a><\/strong><\/p>\n
1. Bock F, Onderka J, Dietrich T, Bac<\/strong>hmann B, Kruse FE, Paschke M, et al. Bevacizumab as a potent inhibitor of inflammatory corneal angiogenesis and lymphangiogenesis. Invest Ophthalmol Vis Sci. 2007;48:2545-52. (ELISA, in vivo control, Surface plasmon resonance)<\/p>\n
2. Zhang J, Jacobi AM, Wang T, Berlin R, Volpe BT, Diamond B. Polyreactive autoantibodies in systemic lupus erythematosus have pathogenic potential. J Autoimmun. 2009;33:270-4. (ELISA)<\/p>\n
3. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (IP)<\/p>\n
4. Ackler S, Mitten MJ, Foster K, Oleksijew A, Refici M, Tahir SK, et al. The Bcl-2 inhibitor ABT-263 enhances the response of multiple chemotherapeutic regimens in hematologic tumors in vivo. Cancer Chemother Pharmacol. 2010;66:869-80. (In vitro control)<\/p>\n
5. Li J, Jie H, Lei Y, Gildener-Leapman N, Trivedi S, Green T, et al. PD-1\/SHP-2 inhibits Tc1\/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment. Cancer Res. 2015;75:508-18. (In vitro control)<\/p>\n
6. Li J, Srivastava RM, Ettyreddy A, Ferris RL. Cetuximab ameliorates suppressive phenotypes of myeloid antigen presenting cells in head and neck cancer patients. J Immunother Cancer. 2015;3:54. (In vitro control, Block)<\/p>\n
7. Woess C, Tuzlak S, Labi V, Drach M, Bertele D, Schneider P, et al. Combined loss of the BH3-only proteins Bim and Bmf restores B-cell development and function in TACI-Ig transgenic mice. Cell Death Differ. 2015;22:1477-88. (In vivo control)<\/p>\n
8. Hotard AL, Lee S, Currier MG, Crowe JE Jr, Sakamoto K, Newcomb DC, et al. Identification of residues in the human respiratory syncytial virus fusion protein that modulate fusion activity and pathogenesis. J Virol. 2015;89:512-22. (FC)<\/p>\n
9. Chapuy CI, Nicholson RT, Aguad MD, Chapuy B, Laubach JP, Richardson PG, et al. Resolving the daratumumab interference with blood compatibility testing. Transfusion. 2015;55:1545-54. (FC)<\/p>\n
10. Ortiz DF, Lansing JC, Rutitzky L, Kurtagic E, Prod’homme T, Choudhury A, et al. Elucidating the interplay between IgG-Fc valency and Fc\u03b3R activation for the design of immune complex inhibitors. Sci Transl Med. 2016;8:365ra158. (Stim)<\/p>\n
11. Liu H, Chumsae C, Gaza-Bulseco G, Hurkmans K, Radziejewski CH. Ranking the susceptibility of disulfide bonds in human IgG1 antibodies by reduction, differential alkylation, and LC-MS analysis. Anal Chem. 2010;82:5219-26. (Mass spectrometry)<\/p>\n","protected":false},"excerpt":{"rendered":"
\u540c\u578b\u5c0d\u7167(Isotype control)-Human IgG1 Kappa \u540c\u578b\u5c0d\u7167(Isotype con […]<\/p>\n","protected":false},"author":3,"featured_media":5349,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[432,431,433,5381],"tags":[4686],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5431"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5431"}],"version-history":[{"count":9,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5431\/revisions"}],"predecessor-version":[{"id":46411,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5431\/revisions\/46411"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5349"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5431"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5431"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5431"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}