{"id":5295,"date":"2016-07-26T16:22:15","date_gmt":"2016-07-26T08:22:15","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=5295"},"modified":"2016-07-28T23:59:34","modified_gmt":"2016-07-28T15:59:34","slug":"%e3%80%90%e6%8a%97%e9%ab%94%e7%b4%94%e5%8c%96%e5%a5%97%e7%b5%84-%e6%8a%97%e9%ab%94%e6%bf%83%e7%b8%ae%e5%8f%8a%e7%b4%94%e5%8c%96-%e3%80%91%e7%a7%bb%e9%99%a4%e6%ba%b6%e6%b6%b2%e4%b8%ad%e6%ae%98","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=5295","title":{"rendered":"\u3010\u6297\u9ad4\u7d14\u5316\u5957\u7d44 | \u6297\u9ad4\u6fc3\u7e2e\u53ca\u7d14\u5316 \u3011\u79fb\u9664BSA\u3001Glycine\u3001Tris\u3001Azide\u7b49\u6b98\u7559\u5206\u5b50"},"content":{"rendered":"

\u6297\u9ad4\u7d14\u5316\u5957\u7d44 | \u6297<\/strong>\u9ad4\u6fc3\u7e2e\u53ca\u7d14\u5316<\/strong> <\/span>\u200b<\/span><\/p>\n

AbSelect Antibody Purification System (860-0010)<\/p>\n

Innova Bioseiences\u63d0\u4f9b\u4e00\u7cfb\u7d71\u6297\u9ad4\u7d14\u5316\u5957\u7d44\uff0c\u6bcf\u7d44\u5957\u7d44\u5305\u62ec\u6297\u9ad4\u7d14\u5316\u4e4bbuffer\u53caresin\uff0c\u6709\u6548\u7d14\u5316\u4e0d\u540c\u4f86\u6e90\u4e4b\u6297\u9ad4\u542b\u91cf\u3002\u5176\u7d14\u5316\u4f86\u6e90\uff0c\u53ef\u70ba\u5e02\u552e\u6297\u9ad4\u4e4b\u7d14\u5316(\u8ca8\u865f#860-0010\/rabbit; \u8ca8\u865f#830-0010\/mouse; \u8ca8\u865f#890-0010\/rat\/sheep\/human\/goat)\u3001\u8179\u6c34\u53ca\u8840\u6e05\u7522\u88fd\u6297\u9ad4\u4e4b\u7d14\u5316(\u8ca8\u865f#863-0500\/rabbit; \u8ca8\u865f#893-0500\/rat\/sheep\/human\/goat\/mouse)<\/a>\u3001\u878d\u5408\u7624\u7d44\u7e54\u57f9\u990a\u6db2(TCS)\u7522\u88fd\u4e4b\u6297\u9ad4(\u8ca8\u865f#862-0500\/rabbit\/human; \u8ca8\u865f#832-0500\/mouse)<\/a>\u3001\u4f4e\u6fc3\u5ea6\u6297\u9ad4\u4e4b\u7d14\u5316\u53ca\u6fc3\u7e2e(\u8ca8\u865f#861-0010)<\/a>\u3002\u6709\u6548\u79fb\u9664\u6eb6\u6db2\u4e2dBSA\u3001Glycine\u3001Tris\u3001Azide\u7b49\u6b98\u7559\u5206\u5b50\uff0c\u7d14\u5316\u5f8c\u6297\u9ad4\u6eb6\u6db2\uff0c\u53ef\u76f4\u63a5\u61c9\u7528\u65bclateral flow test\u5948\u7c73\u91d1\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (Innova gold,#230-0010, innova biosciences)\u3001\u8207HRP\/\u87a2\u5149\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44(Innova lightning-link, #701-0000)\u3002<\/p>\n

\u200b<\/span>\"\"\u200b<\/span><\/span><\/span>\u200b<\/span><\/span>\u200b<\/span><\/p>\n

\u6297\u9ad4\u6fc3\u7e2e\u53caClean up\u7cfb\u7d71 <\/strong>\u9069\u5408\u61c9\u7528: \u4f4e\u6fc3\u5ea6\u6297\u9ad4\u4e4b\u7d14\u5316\u53ca\u6fc3\u7e2e\u3002<\/p>\n

\u6297\u9ad4\u4fdd\u5b58\u6db2\u4e2d\uff0c\u6709\u6642\u6703\u5b58\u5728\u9ad8\u6fc3\u5ea6\u4e4b\u86cb\u767d\u8cea\uff0c\u5e72\u64fe\u9175\u7d20\u578bHRP\u3001Biotin\u3001streptavidin\u53caFITC\u87a2\u5149\u7b49\u4e4b\u6297\u9ad4\u6a19\u8a8c\u53cd\u61c9\u3002\u53ef\u5c07\u9810\u6a19\u8a8c\u7684\u6297\u9ad4\uff0c\u9032\u4e00\u6b65\u79fb\u9664\u6eb6\u6db2\u4e2d\u6b98\u7559\u4e4bamino group\u3002\u7d14\u5316\u5f8c\u4e4b\u6297\u9ad4\uff0c\u53ef\u76f4\u63a5\u9032\u884c\u5948\u7c73\u91d1\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (Innovagold,#230-0010, innova biosciences)\u53ca\u6297\u9ad4\u87a2\u5149\u6216HRP\u6a19\u8a8c(lightning_link)\u3002<\/p>\n

\u6297\u9ad4\u6fc3\u7e2e\u53caClean up\u6d41\u7a0b<\/strong><\/p>\n

Step 1 \u52a0\u5165 0.5ml \u4ee5\u4e0a\u7684\u6297\u9ad4\u91cf\u81f3 spin cartridge.<\/p>\n

Step 2 15,000g<\/em>\u96e2\u5fc3 1 to 3 \u5206, \u4f7f\u6297\u9ad4\u91cf\u964d\u4f4e\u81f3100ul<\/p>\n

Step 3 \u5438\u6389\u96e2\u5fc3\u7ba1\u7684\u6eb6\u6db2<\/p>\n

Step 4 \u52a0\u5165 400\u03bcl conjugation buffer\u81f3spin cartridge\u4e2d\u7684\u6297\u9ad4<\/p>\n

Step 5 15,000g<\/em>\u96e2\u5fc3 1 to 3 \u5206, \u4f7f\u6297\u9ad4\u91cf\u964d\u4f4e\u81f3100ul<\/p>\n

Step 6\u5438\u6389\u96e2\u5fc3\u7ba1\u7684\u6eb6\u6db2.<\/p>\n

Step 7 \u91cd\u89861-6\u6b65\u9a5f, \u7f6e\u63db\u6297\u9ad4\u6eb6\u6db2\u3002<\/p>\n

Step 8 \u7531spin cartridge\u4e2d\u56de\u6536\u7d14\u5fc3\u5f8c\u7684\u6297\u9ad4<\/p>\n

Antibody Concentration and Cleanup Kit<\/strong><\/a><\/p>\n

Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.<\/p>\n

The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.<\/p>\n

DIY<\/strong>\u6297\u9ad4\u6a19\u8a8c\u87a2\u5149<\/strong>(FITC,PE,Cy3,Cy5,Cy7 dye)<\/strong>\u6ce8\u610f\u4e8b\u9805<\/p>\n

\u8acb\u78ba\u8a8d\u6297\u9ad4\u7684\u4fdd\u5b58\u689d\u4ef6\u3002\u50b3\u7d71\u5e02\u552e\u7684\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44\uff0c\u5728\u4f7f\u7528\u524d\u7686\u9700dialysis\u7d14\u5316\uff0c\u5c07\u6297\u9ad4\u4fdd\u5b58\u7684\u7269\u8cea\u79fb\u9664\u3002Lightning-link\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44\u5728\u5927\u90e8\u4efd\u7684\u4fdd\u5b58\u689d\u4ef6\u4e0b\uff0c\u4e0d\u9700\u8981\u518ddialysis\u7684\u6b65\u9a5f\uff0c\u4f8b\u5982\u4e00\u822csalts (e.g. NaCl)\u3001sugars(e.g. sucrose)\u3001\u53cachelators (e.g. EDTA)\u4e0d\u6703\u5f71\u97ff\u6a19\u8a8c\u7684\u6d3b\u6027\u3002\u5927\u90e8\u7684\u5e02\u552e\u7684\u6297\u9ad4\u4fdd\u5b58\u57281%BSA\u30010.5%Azide\u53ca0.5% Gelatin \u8f15\u5fae\u6216\u5e7e\u4e4e\u4e0d\u6703\u5f71\u97ff\u6a19\u8a8c\u7684\u6d3b\u6027\u3002\u5728\u5c11\u6578\u60c5\u6cc1\u4e0b\uff0c\u82e5\u662f\u60a8\u8cfc\u8cb7\u7684\u6297\u9ad4\u4fdd\u5b58\u5728amino acids(e.g. glycine)\u3001blockers(e.g. ethanolamine)\u53cathiols(DTT, mercaptoethanol)\u60c5\u6cc1\u4e0b\uff0c\u8a18\u5f97\u7d14\u5316\u60a8\u7684\u6297\u9ad4\uff0c(abselect\u6297\u9ad4\u7d14\u5316\u5957\u7d44) \u907f\u514d\u6291\u5236\u60a8\u7684\u6a19\u8a8c\u6d3b\u6027\u4e86!<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
AbSelect Antibody Concentration and Clean-Up Kit (861-0010)<\/a><\/td>\n<\/tr>\n
AbSelect Rat TCS Purification System (842-0005)<\/a><\/td>\n<\/tr>\n
AbSelect BSA Removal Kit (820-0100)<\/a><\/td>\n<\/tr>\n
AbSelect Mouse Antibody Purification System (830-0010)<\/a><\/td>\n<\/tr>\n
AbSelect Antibody Purification System (860-0010)<\/a><\/td>\n<\/tr>\n
AbSelect G Antibody Purification System (890-0010)<\/a><\/td>\n<\/tr>\n
AbSelect Mouse TCS Purification System (832-0500)<\/a><\/td>\n<\/tr>\n
AbSelect TCS Antibody Purification System (862-0500)<\/a><\/td>\n<\/tr>\n
AbSelect Serum Antibody Purification System (863-0500)<\/a><\/td>\n<\/tr>\n
\n

AbSelect G Serum Antibody Purification System (893-0500)<\/a><\/p>\n

Protein A Agarose (851-0024)<\/a><\/p>\n

Protein G Agarose (895-0024)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u6297\u9ad4\u7d14\u5316\u5957\u7d44 | \u6297\u9ad4\u6fc3\u7e2e\u53ca\u7d14\u5316 \u200b AbSelect Antibody Purification Syste […]<\/p>\n","protected":false},"author":3,"featured_media":5013,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[151,442,188,441,432,431,3,440],"tags":[439],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5295"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5295"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5295\/revisions"}],"predecessor-version":[{"id":5300,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/5295\/revisions\/5300"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5013"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5295"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}