{"id":52912,"date":"2025-09-05T16:16:41","date_gmt":"2025-09-05T08:16:41","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=52912"},"modified":"2025-11-28T10:44:28","modified_gmt":"2025-11-28T02:44:28","slug":"%e8%84%af%e8%83%ba%e9%85%b8%ef%bc%88proline-pro%ef%bc%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-chekine-micro-proline-pro-assay-kit-%e8%b2%a8%e8%99%9fktb1430","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=52912","title":{"rendered":"\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Micro Proline (PRO) Assay Kit \u8ca8\u865fKTB1430"},"content":{"rendered":"

\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Micro Proline (PRO) Assay Kit \u8ca8\u865fKTB1430<\/span><\/a>\u00a0<\/span><\/strong>(\u9748\u654f\u5ea60.5-80 \u00b5g\/mL)<\/span><\/span><\/p>\n

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\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 (CheKine\u2122 Micro Proline (PRO) Assay Kit, KTB1430) \u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\uff0c\u53ef\u6aa2\u6e2c\u8840\u6e05(serum)\u3001\u8840\u6f3f( plasma)\u3001\u52d5\u690d\u7269\u7d44\u7e54\u3001\u7d30\u80de\u548c\u7d30\u83cc(bacteria)\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u672c\u4e2d\u7684\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u542b\u91cf\u3002\u6e2c\u5b9a\u904e\u7a0b\u4e2d\uff0c\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u7528\u78fa\u57fa\u6c34\u694a\u9178\uff08sulfosalicylic acid, SA\uff09\u8403\u53d6\u3002\u52a0\u71b1\u8655\u7406\u5f8c\uff0c\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u8207\u9178\u6027\u831a\u4e09\u916e\u6eb6\u6db2(acidic ninhydrin solution)\u53cd\u61c9\u751f\u6210\u7d05\u8272\uff1b\u7528\u7532\u82ef( toluen)\u8403\u53d6\u5f8c\uff0c\u5728520nm\u8655\u6e2c\u91cf\u5438\u5149\u5ea6\u3002\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269(animals,)\u3001\u690d\u7269( plants)\u3001\u5fae\u751f\u7269(microorganisms)\u53ca\u57f9\u990a\u7d30\u80de\u3002\u9006\u5883\u689d\u4ef6(adversity conditions)\u4e0b\uff0c\u690d\u7269\u9ad4\u5167\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u542b\u91cf\u986f\u8457\u5347\u9ad8\u3002\u812f\u80fa\u9178\uff08Proline, PRO\uff09\u542b\u91cf\u7684\u5347\u9ad8\u5728\u67d0\u7a2e\u7a0b\u5ea6\u4e0a\u53cd\u6620\u4e86\u690d\u7269\u7684\u6297\u9006\u6027\uff0c\u6297\u65f1\u7684\u54c1\u7a2e\u5f80\u5f80\u7d2f\u7a4d\u66f4\u591a\u7684\u812f\u80fa\u9178\u3002\u56e0\u6b64\uff0c\u812f\u80fa\u9178\u542b\u91cf\u7684\u5347\u9ad8\u53ef\u4ee5\u4f5c\u70ba\u6297\u9006\u80b2\u7a2e\u7684\u751f\u7406\u6307\u6a19\u4e4b\u4e00\u3002<\/p>\n

Applicable samples: Plant Tissues, Animal Tissues, Cells, Bacteria, Serum, Plasma<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Proline (PRO) Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nThe kit provides a simple method for detecting Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria. In the assay, Pro is extracted with sulfosalicylic acid (SA). After heat treatment, Pro reacts with acidic ninhydrin solution to produce red; after extraction with toluene, the absorbance is measured at 520 nm.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Extraction Buffer
\n\u2022 Assay Buffer I
\n\u2022Chromogen
\n\u2022Pro Substrate<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria.
\n\u2022 A broad range linearity: 0.5-80 \u03bcg\/mL.
\n\u2022 Detailed sample preparation and result calculation methods are provided.
\n\u2022 Simple operation steps.
\n\u2022 Standard and standard curve line are provided.<\/td>\n<\/tr>\n
Calibration range<\/strong><\/td>\n0.5-80 \u03bcg\/mL<\/td>\n<\/tr>\n
Limit of detection<\/strong><\/td>\n0.5 \u03bcg\/mL<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.
\n\u2022 Avoid foaming or bubbles when mixing or reconstituting components.
\n\u2022 Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before starting the assay.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nPro is widely present in animals, plants, microorganisms and cultured cells. Under adversity conditions, the Pro content in plants increased significantly. The increased amount of Pro reflects stress resistance to a certain extent, and varieties with strong drought resistance tend to accumulate more proline. Therefore, the increased amount of proline can be used as one of the physiological indicators of stress resistance breeding.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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