{"id":50378,"date":"2024-10-18T18:13:14","date_gmt":"2024-10-18T10:13:14","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=50378"},"modified":"2025-04-08T13:10:02","modified_gmt":"2025-04-08T05:10:02","slug":"blyscan-%e7%a1%ab%e9%85%b8%e5%8c%96%e7%b3%96%e8%83%ba%e8%81%9a%e9%86%a3-sgag-%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-sulfated-glycosaminoglycan-sgag-assay-kit-%e8%b2%a8%e8%99%9fbly","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=50378","title":{"rendered":"Blyscan\u2122 \u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3 (sGAG) \u6aa2\u6e2c\u8a66\u5291\u76d2| sulfated Glycosaminoglycan (sGAG) assay kit \u8ca8\u865fBlyscan B1000\/B3000"},"content":{"rendered":"

Blyscan\u2122 \u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3 (sGAG) \u6aa2\u6e2c\u8a66\u5291\u76d2| sulfated Glycosaminoglycan (sGAG) assay kit \u8ca8\u865fBlyscan B1000\/B3000<\/span><\/a><\/strong><\/span><\/p>\n

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Blyscan\u2122 \u7cd6\u80fa\u805a\u91a3\u8a66\u5291\u76d2\u4e00\u76f4\u662f\u53ef\u9760 sGAG \u548c\u86cb\u767d\u805a\u91a3\u5206\u6790\u7684\u300c\u9996\u9078\u300d\u89e3\u6c7a\u65b9\u6848\uff01 Blyscan \u5229\u7528\u67d3\u6599\u7d50\u5408\u65b9\u6cd5\u5b9a\u91cf\u6e2c\u91cf\u5404\u7a2e\u9ad4\u5167\u548c\u9ad4\u5916\u4f86\u6e90\u7684\u7d30\u80de\u3001\u7d44\u7e54\u548c\u6db2\u9ad4\u4e2d\u7684\u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3 (sGAG) \u548c\u86cb\u767d\u805a\u91a3\u3002Our Blyscan\u2122 Glycosaminoglycan Kit has been a \u2018go-to\u2019 Solution for reliable sGAG and Proteoglycan Analysis for many years! Blyscan utilises a dye-binding approach to quantitatively measure sulfated glycosaminoglycans (sGAG) and proteoglycans in cells, tissues and fluids from a wide range of in-vivo and in-vitro sources.<\/p>\n

Blyscan \u63d0\u4f9b\u4e4b\u7cd6\u80fa\u805a\u91a3\u8a66\u5291\u76d2 (Blyscan\u2122 Glycosaminoglycan Kit ,B1000\/B3000) \u662f\u4e00\u7a2e\u5b9a\u91cf\u67d3\u6599\u7d50\u5408\u65b9\u6cd5\uff0c\u7528\u65bc\u5206\u6790\u786b\u9178\u5316\u86cb\u767d\u805a\u91a3(sulfated proteoglycans )\u548c\u91a3\u80fa\u805a\u91a3 (glycosaminoglycans, sGAG)\u3002\u7576\u6e2c\u8a66\u6750\u6599\u4ee5\u53ef\u6eb6\u5f62\u5f0f\u5b58\u5728\u6642\uff0c\u6216\u5f9e\u751f\u7269\u6750\u6599\u4e2d\u63d0\u53d6\u6728\u74dc\u86cb\u767d\u9176(papain extraction)\u5f8c\uff0c\u53ef\u4ee5\u76f4\u63a5\u9032\u884c\u6e2c\u5b9a\u3002 \u6b64\u6e2c\u5b9a\u53ef\u7528\u65bc\u6e2c\u91cf\u7e3d sGAG \u542b\u91cf(total sGAG content )\uff0c\u4e5f\u53ef\u7528\u65bc\u6e2c\u5b9a\u6e2c\u8a66\u6a23\u54c1\u4e2d O- \u548c N- \u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3(N-sulfated glycosaminoglycan)\u7684\u6bd4\u4f8b\u3002 \u6b64\u6e2c\u5b9a\u4e2d\u4f7f\u7528\u7684\u67d3\u6599\u6a19\u8a18\u662f1, 9-\u4e8c\u7532\u57fa\u4e9e\u7532\u85cd(1, 9-dimethylmethylene blue)\uff0c\u4e26\u4e14\u8a72\u67d3\u6599\u5728\u70ba\u86cb\u767d\u805a\u91a3(proteoglycans )\u7684\u786b\u9178\u5316\u591a\u91a3\u7d44\u5206\u6216\u7121\u86cb\u767d\u8cea\u7684\u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3\u93c8(sulfated glycosaminoglycan chains)\u63d0\u4f9b\u7279\u5b9a\u6a19\u8a18\u7684\u689d\u4ef6\u4e0b\u4f7f\u7528\u3002 \u6b64\u6e2c\u5b9a\u4e0d\u9069\u7528\u65bc\u5c0f\u7684\u786b\u9178\u5316\u4e8c\u7cd6\u7247\u6bb5(sulfated disaccharide fragments )\u6216\u542b\u6709\u85fb\u9178\u9e7d( alginates)\u7684\u6a23\u54c1\uff0c\u56e0\u70ba\u9019\u4e9b\u6a23\u54c1\u542b\u6709\u91a3\u919b\u9178(uronic acid)\u3002<\/p>\n

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\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

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\u91a3\u80fa\u805a\u91a3 (GAG) \u548c\u86cb\u767d\u805a\u91a3 ( Glycosaminoglycans (GAGs) and Proteoglycans )<\/strong><\/span><\/h3>\n

\u91a3\u80fa\u805a\u91a3 (Glycosaminoglycans, GAG) \u662f\u4e00\u7a2e\u5e36\u8ca0\u96fb\u8377\u7684\u591a\u91a3 (negatively charged polysaccharide )\uff0c\u5728\u5404\u7a2e\u751f\u7269\u904e\u7a0b\u4e2d\u767c\u63ee\u8457\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u5b83\u5011\u7531\u91cd\u8907\u7684\u4e8c\u7cd6\u55ae\u5143(disaccharide units)\u7d44\u6210\uff0c\u901a\u5e38\u7531 N-\u4e59\u91af\u5316( N<\/em>-acetylated )\u6216 N-\u786b\u9178\u5316\u5df1\u7cd6\u80fa(N<\/em>-sulfated hexosamine)\u8207\u91a3\u919b\u9178\uff08GlcA \u6216 IdoA\uff09\u6216\u534a\u4e73\u7cd6\u914d\u5c0d\u3002\u4e5f\u53ef\u4ee5\u6dfb\u52a0\u786b\u9178\u57fa\u5718\uff0c\u4f7f\u786b\u9178\u5316 GAG \u5e36\u6709\u6574\u9ad4\u8ca0\u96fb\u8377\uff0c\u5f9e\u800c\u5f71\u97ff\u7d30\u80de\u76f8\u4e92\u4f5c\u7528\uff0c\u4e26\u4e14\u9084\u53ef\u4ee5\u900f\u904e\u6211\u5011\u7684 Blyscan \u67d3\u6599\u8a66\u5291\u7d50\u5408\u3002GAG \u7684\u5e38\u898b\u4f8b\u5b50\u5305\u62ec\u786b\u9178\u8edf\u9aa8\u7d20(Chondroitin Sulfate)\u3001\u786b\u9178\u76ae\u819a\u7d20(Dermatan Sulfate)\u3001\u809d\u7d20(Heparin)\u3001\u786b\u9178\u4e59\u91af\u809d\u7d20(Heparan Sulfate)\u548c\u786b\u9178\u89d2\u8cea\u7d20(Keratan Sulfate)\u3002\u8acb\u6ce8\u610f\uff0c\u900f\u660e\u8cea\u9178(Hyaluronic Acid )\u662f\u4e00\u7a2e\u975e\u786b\u9178\u5316 GAG\uff0c\u7121\u6cd5\u900f\u904e Blyscan \u6e2c\u5b9a\u6cd5\u6aa2\u6e2c\u3002\u5982\u679c\u60a8\u9700\u8981\u6e2c\u91cf\u900f\u660e\u8cea\u9178(Hyaluronic Acid )\uff0c\u6211\u5011\u5efa\u8b70\u4f7f\u7528\u6211\u5011\u7684 Purple-Jelley \u8a66\u5291\u76d2\uff01<\/p>\n

\u7cd6\u80fa\u805a\u91a3\u5728\u7d44\u7e54\u4e2d\u7684\u4f5c\u7528 (The Role of Glycosaminoglycans in Tissues)<\/strong><\/span><\/h3>\n

\u91a3\u80fa\u805a\u91a3 (Glycosaminoglycans, GAG) \u548c\u86cb\u767d\u805a\u91a3\u5728\u7d44\u7e54\u548c\u751f\u7269\u9ad4\u4e2d\u5177\u6709\u91cd\u8981\u529f\u80fd\uff0c\u900f\u904e\u652f\u67b6\u63d0\u4f9b\u751f\u7269\u7269\u7406\u652f\u6301\u4e26\u7dad\u6301\u8edf\u9aa8\u6c34\u5408\u3002\u5b83\u5011\u5728\u7d30\u80de\u9ecf\u9644( cell adhesion)\u548c\u4fe1\u865f\u50b3\u5c0e( signalling)\u7b49\u751f\u5316\u904e\u7a0b\u4e2d\u4e5f\u767c\u63ee\u8457\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002<\/p>\n

\u200dWhat is the origin of the Blyscan assay name?<\/strong><\/span><\/span><\/h3>\n

Blyscan \u662f\u4e00\u500b\u53e4\u82f1\u8a9e\u55ae\u8a5e\uff0c\u610f\u601d\u662f\u201c\u9583\u8000\u201d\uff0c\u201cblush\u201d\uff08\u81c9\u7d05\uff09\u4e00\u8a5e\u53ef\u80fd\u6e90\u81ea\u65bc\u8a72\u55ae\u5b57\u3002\u9019\u662f\u4e00\u500b\u5408\u9069\u7684\u9078\u64c7\uff0c\u56e0\u70ba Blyscan \u6aa2\u6e2c\u542b\u6709\u4e00\u7a2e\u85cd\u8272\u67d3\u6599\uff0c\u7576\u5b83\u8207\u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3\u7d50\u5408\u6642\u6703\u300c\u8b8a\u6210\u300d\u4eae\u7c89\u200b\u200b\u8272\uff01<\/p>\n

\u6e2c\u5b9a\u6a23\u672c\u6750\u6599 \u53ef\u6eb6\u6027\u8403\u53d6\u7269\uff1a<\/p>\n

\u5982\u7e96\u7dad\u8edf\u9aa8(fibrous)\u548c\u900f\u660e\u8edf\u9aa8(hyaline cartilages)\u3002\u52d5\u8108(arteriesarteries)\u3001\u5fc3\u81df\u3001\u80ba\u3001\u76ae\u819a\u548c\u5176\u4ed6\u542b\u6709\u7d30\u80de\u5916\u57fa\u8cea\uff08\u7d50\u7de0\u7d44\u7e54\uff09\u7684\u7269\u8cea\u548c\u5be6\u9ad4\u816b\u7624\u3002\u7d30\u80de\u5916\u57fa\u8cea\u6210\u5206\u53ef\u80fd\u7531\u6d3b\u7d30\u80de\u91cb\u653e\u5230\u57f9\u990a\u57fa\u4e2d\uff0c\u5176\u4e2d\u4e00\u4e9b\u53ef\u4ee5\u9644\u8457\u5728\u7d30\u80de\u57f9\u990a\u5851\u81a0\u5668\u76bf\u4e0a\u3002\u4f86\u81ea\u6ed1\u6db2\u3001\u5c3f\u6db2\u548c\u51dd\u81a0\u8272\u8b5c\u7d1a\u5206\u7b49\u4efd\u7684\u53ef\u6eb6\u6027 sGAG\u3002\u6e2c\u8a66\u6a23\u54c1\u6210\u5206\u4f7f\u7528\u8a72\u6e2c\u5b9a\u6cd5\u6e2c\u91cf\u4e86\u5ee3\u6cdb\u7684\u54fa\u4e73\u52d5\u7269\u86cb\u767d\u591a\u91a3\u3002 \u4ee5\u4e0bsGAG\uff0c\u7121\u8ad6\u662f\u4ecd\u9644\u8457\u5728\u80dc\u80bd\/\u86cb\u767d\u8cea\u6838\u5fc3\u4e0a\uff0c\u6216\u662f\u4f5c\u70ba\u904a\u96e2\u93c8\u90fd\u53ef\u4ee5\u9032\u884c\u6e2c\u5b9a\uff1a \u786b\u9178\u8edf\u9aa8\u7d20\uff084- \u548c6-\u786b\u9178\u5316\uff09 \u786b\u9178\u89d2\u8cea\u7d20\uff08\u9e7c\u654f\u611f\u548c\u6297\u6027\u5f62\u5f0f\uff09 \u786b\u9178\u76ae\u819a\u7d20\u786b\u9178\u4e59\u91af\u809d\u7d20\uff08\u5305\u62ec\u809d\u7d20\uff09 \u900f\u660e\u8cea\u9178\u9e7dBlyscan \u6aa2\u6e2c\u672a\u6aa2\u6e2c\u5230\u3002 \u6e2c\u8a66\u6a23\u54c1\u4e2d\u900f\u660e\u8cea\u9178\u7684\u5b58\u5728\u4e0d\u6703\u5e72\u64fe sGAG \u7684\u6e2c\u91cf\u3002\u7528\u65bc\u5206\u6790\u7684\u6a23\u672c\u5fc5\u9808\u4e0d\u542b\u4efb\u4f55\u9846\u7c92\u7269\u6216\u6df7\u6fc1\u7269\uff08\u7d30\u80de\u788e\u7247\u3001\u4e0d\u6eb6\u6027\u7d30\u80de\u5916\u57fa\u8cea\u6750\u6599\uff09\u3002 \u7d44\u7e54\u8403\u53d6\u7269\u4e2d\u5b58\u5728\u7684\u53ef\u6eb6\u6027\u6210\u5206\uff08\u4f8b\u5982\u86cb\u767d\u8cea\u548c\u4e2d\u6027\u78b3\u6c34\u5316\u5408\u7269\uff09\u4e0d\u6703\u5e72\u64fe\u6e2c\u5b9a\u3002
\n<\/p>\n

Fig. 3: A CHO cell line cultured for 72 hours, without a culture medium change, (cells confluent). The 12\/24\/96 well plates and T25 flasks were treated with papain to recover sGAG.<\/p>\n

<\/p>\n

Fig. 4: CHO cells cultured in a T-25 flask.<\/p>\n

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Fig. 5: Molecular Structure of Blyscan Dye.<\/p>\n

<\/p>\n

Fig. 6: Absorption Spectrum of Blyscan Dye.<\/p>\n

<\/p>\n

Fig.7: A typical straight line assay calibration curve using chondroitin 4-sulfate (bovine trachea). Limits of Detection Using a 100\u00b5l sample aliquot, a 0.25\u00b5g quantity of chondroitin 4sulfate (equivalent to 2.5 \u00b5g\/ml) gives a 656nm absorbance value of ~0.10. The maximum 656nm absorbance value for 5\u00b5g (equivalent to 50 \u00b5g\/ml) sGAG is ~1.0. Higher absorbance values will depart from a linear correlation between absorbance and concentration.<\/p>\n

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Fig.8: Proposed mechanism for the nitrous acid cleavage of N-sulfated glycosaminoglycans (heparan sulfate and heparin) (Carney,S.L.,1986).<\/p>\n

<\/p>\n

Fig. 9: A typical calibration curve suitable for the determination of the O-sulfated and N-sulfated glycosaminoglycan content of a test sample.<\/p>\n

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Fig. 10 Blyscan Assay; step-by-step (a) 0 and 5\u00b5g of sGAG and Blyscan Dye, (after 15 minutes mixing). (b) 30 min mixing and then centrifuged, (note the sGAG-Dye pellet). (c) The non-sGAG Dye was drained from tubes with pellet retained. (d) Dye released from sGAG using the Dye Dissociation Reagent.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Blyscan\u2122 \u786b\u9178\u5316\u7cd6\u80fa\u805a\u91a3 (sGAG) \u6aa2\u6e2c\u8a66\u5291\u76d2| sulfated Glycosaminoglyc […]<\/p>\n","protected":false},"author":3,"featured_media":50379,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[6804],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/50378"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=50378"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/50378\/revisions"}],"predecessor-version":[{"id":50399,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/50378\/revisions\/50399"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/50379"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=50378"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=50378"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=50378"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}