{"id":47141,"date":"2023-11-17T11:59:45","date_gmt":"2023-11-17T03:59:45","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=47141"},"modified":"2023-11-27T16:52:42","modified_gmt":"2023-11-27T08:52:42","slug":"%e7%b2%92%e7%b7%9a%e9%ab%94%e6%bb%b2%e9%80%8f%e6%80%a7transition-pore%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fkta4002","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=47141","title":{"rendered":"\u7c92\u7dda\u9ad4\u6ef2\u900f\u6027Transition Pore\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTA4002(Ex\/Em=494\/517 nm)"},"content":{"rendered":"

\u7c92\u7dda\u9ad4\u6ef2\u900f\u6027Transition Pore\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/strong> | \u8ca8\u865fKTA4002 (Ex\/Em=494\/517 nm)<\/span><\/strong><\/a><\/span><\/p>\n

This kit(100T) can detect 1,000T with 100\u03bcL of detection system per wel lin 96-well plate.<\/p>\n

Mitochondrial Permeability Transition Pore Assay Kit
\n<\/em><\/strong><\/em><\/strong><\/p>\n

\u7c92\u7dda\u9ad4(Mitochondria)<\/em>\u662f\u7d30\u80de\u7684\u529f\u7387\u4e2d\u5fc3(power centrals)<\/em>\uff0c\u5728\u80fd\u91cf\u751f\u7522\u4e2d\u8d77\u8457\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u7c92\u7dda\u9ad4\u7684\u640d\u50b7( Damage)<\/em>\u6fc0\u6d3b\u8a98\u5c0e\u7d30\u80de\u51cb\u4ea1\u7684\u4fe1\u865f\u901a\u8def(signaling pathways)<\/em>\u3002\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\uff08Mitochondrial permeablity transition pore, MPTP\uff09\u53c8\u7a31\u78c1\u901a\u9053(magachannel)\uff0c\u662f\u5b58\u5728\u65bc\u7c92\u7dda\u9ad4(mitochondria)\u5167\u5916\u819c\u4e4b\u9593\u7684\u975e\u9078\u64c7\u6027\u9ad8\u96fb\u5c0e\u7387\u901a\u9053(high conductivity channel)\uff0c\u7531\u591a\u7a2e\u86cb\u767d\u8cea\u8907\u5408\u7269\u7d44\u6210(multiple protein complexes)\u3002\u662f\u7531\u5167\u7dda\u7c92\u9ad4\u819c\u548c\u5916\u7dda\u7c92\u9ad4\u819c\u7d44\u6210\u7684\u975e\u7279\u7570\u6027\u901a\u9053(non-specific channel formed)\u3002<\/em><\/p>\n

\u4f3c\u4e4e\u5728\u7d30\u80de\u6b7b\u4ea1\u671f\u9593\u53c3\u8207\u7c92\u7dda\u9ad4\u6210\u5206\u7684\u91cb\u653e\u3002 \u5728\u5065\u5eb7\u7d30\u80de\u4e2d\uff0c\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\uff08mitochondrial permeability transition pore, <\/em>MPT\u5b54\u6216MPTP\uff09\u5728\u958b\u653e\u72c0\u614b\u548c\u9589\u5408\u72c0\u614b\u4e4b\u9593\u9583\u720d\uff0c\u4f46\u662f\u5728\u7d30\u80de\u6b7b\u4ea1\u904e\u7a0b\u4e2d\uff0c\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\uff08mitochondrial permeability transition pore, <\/em>MPT\u5b54\u6216MPTP\uff09\u986f\u8457\u6539\u8b8a\u4e86\u7c92\u7dda\u9ad4\u7684\u901a\u900f\u6027\u3002 \u7d30\u80de\u8272\u7d20C\u91cb\u653e(Cytochrome c release)<\/em>\u548c\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d( mitochondrial membrane potential )<\/em>\u7684\u55aa\u5931\u662f\u9023\u7e8c\u5b54\u6d3b\u5316\u5f8c\u7684\u3002\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\uff08 mitochondrial permeability transition pore, mPTP\u6216MPTP\uff1b\u4e5f\u7a31\u70baPTP\u3001mTP\u6216MTP\uff09\u662f\u5728\u67d0\u4e9b\u75c5\u7406\u689d\u4ef6\u4e0b, \u4f8b\u5982\u5275\u50b7\u6027\u8166\u640d\u50b7( traumatic brain injury)\u548c\u4e2d\u98a8(stroke)\u5728\u7c92\u7dda\u9ad4\u5167\u819c\u4e2d\u5f62\u6210\u7684\u86cb\u767d\u8cea\u3002\u958b\u653e\u53ef\u4ee5\u589e\u52a0\u7c92\u7dda\u9ad4\u819c\u5c0d\u5206\u5b50\u91cf\u5c0f\u65bc 1500 \u9053\u723e\u9813\u7684\u5206\u5b50\u7684\u6ef2\u900f\u6027\u3002\u6839\u64da\u7279\u5b9a\u7684\u751f\u7269\u74b0\u5883\uff0c\u901a\u900f\u6027\u8f49\u8b8a\u5b54\u3001\u7c92\u7dda\u9ad4\u819c\u901a\u900f\u6027\u8f49\u8b8a\uff08mPT \u6216 MPT\uff09\u7684\u8a98\u5c0e\u53ef\u5c0e\u81f4\u7c92\u7dda\u9ad4\u816b\u8139( mitochondrial swelling)\u548c\u7d30\u80de\u900f\u904e\u51cb\u4ea1\u6216\u58de\u6b7b\u800c\u6b7b\u4ea1( cell death)\u3002\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\uff08Mitochondrial permeablity transition pore, MPTP\uff09\u53ef\u80fd\u53c3\u8207\u7d30\u80de\u6b7b\u4ea1\u904e\u7a0b\u4e2d\u7c92\u7dda\u9ad4\u6210\u5206\u7684\u91cb\u653e\u3002\u7d30\u80de\u6b63\u5e38\u7684\u7c92\u7dda\u9ad4\u5167\u819c\u53ef\u4ee5\u7dad\u6301\u6b63\u5e38\u7684\u7c92\u7dda\u9ad4\u96fb\u4f4d\u68af\u5ea6(mitochondrial potential gradient )\uff0c\u78ba\u4fdd\u7d30\u80de\u7684\u547c\u5438\u548c\u80fd\u91cf\u4f9b\u61c9\u3002\u96a8\u8457Ca2+\u7684\u651d\u5165\u548c\u91cb\u653e\uff0c\u4f4e\u96fb\u5c0e\u7387\u6ef2\u900f\u6027\u904e\u6e21\u5b54\u5728\u6253\u958b\u548c\u95dc\u9589\u4e4b\u9593\u4f86\u56de\u5207\u63db\u3002\u5728\u7d30\u80de\u51cb\u4ea1(apoptosis )\u548c\u75c5\u7406\u6027\u6b7b\u4ea1(pathological death)\u904e\u7a0b\u4e2d\uff0c\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u50b3\u905e\u5b54( mitochondrial membrane potential transfer pore changes)\u7684\u901a\u900f\u6027(permeability)\u767c\u751f\u8b8a\u5316\uff0cCa2+\u8d85\u8f09\uff0c\u7c92\u7dda\u9ad4\u7a40\u80f1\u7518\u80bd\u6c27\u5316\uff0c\u6d3b\u6027\u6c27\u589e\u52a0\uff0c\u5305\u62ec\u96a8\u5f8c\u7d30\u80de\u8272\u7d20C\u7684\u91cb\u653e\uff0c\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u4e0b\u964d\u7b49\u90fd\u6703\u5c0e\u81f4MPTP\u7684\u555f\u52d5\u3002\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u8b8a\u5b54\u6aa2\u6e2c\u8a66\u5291\u76d2(Mitochondrial Permeability Transition Pore Assay Kit)\u662f\u4e00\u7a2e\u6bd4\u50c5\u57fa\u65bc\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u5206\u6790\u66f4\u76f4\u63a5\u7684\u6aa2\u6e2c MPTP \u958b\u53e3\u7684\u6aa2\u6e2c\u65b9\u6cd5\u3002<\/p>\n

\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u8b8a\u5b54\u6aa2\u6e2c\u7522\u54c1\u4ecb\u7d39:<\/span><\/strong><\/span><\/p>\n

\u5728\u7c92\u7dda\u9ad4\u9223\u8d85\u8f09\u7684\u689d\u4ef6\u4e0b\uff0c\u7279\u5225\u662f\u7576\u4f34\u96a8\u6c27\u5316\u58d3\u529b\u3001\u78f7\u9178\u9e7d\u6fc3\u5ea6\u5347\u9ad8\u548c\u817a\u560c\u5464\u6838\u82f7\u9178\u6d88\u8017\u6642\uff0c\u7c92\u7dda\u9ad4\u5167\u819c\u4e0a\u6703\u6253\u958b\u4e00\u500b\u975e\u7279\u7570\u6027\u5b54\uff0c\u5373\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\uff08 MPTP\uff09\u3002\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u8b8a\u5b54\u6aa2\u6e2c\u8a66\u5291\u76d2(Mitochondrial Permeability Transition Pore Assay Kit) \u662f\u6bd4\u50c5\u57fa\u65bc\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u5206\u6790\u66f4\u76f4\u63a5\u7684\u6aa2\u6e2cMPTP\u958b\u653e\u7684\u6aa2\u6e2c\u65b9\u6cd5\u3002\u5176\u539f\u7406\u662f\uff1a\u9996\u5148\u900f\u904e\u88ab\u52d5\u904b\u8f38(passive transportation)\u4f86\u52a0\u8f09Calcein AM\uff0cCalcein AM\u662f\u4e00\u7a2e\u7528\u65bc\u6d3b\u7d30\u80de\u87a2\u5149\u6a19\u8a18\u7684\u7d30\u80de\u67d3\u8272\u8a66\u5291\u3002\u5b83\u5f88\u5bb9\u6613\u7a7f\u900f\u6d3b\u7d30\u80de\u819c\uff0c\u88ab\u7d30\u80de\u5167\u916f\u9176\u88c2\u89e3\uff0c\u5f62\u6210\u4e0d\u900f\u819c\u7684\u6975\u6027\u5206\u5b50\u9223\u9ec3\u7da0\u7d20\uff0c\u7136\u5f8c\u88ab\u6355\u7372\u5728\u7d30\u80de\u5167\uff0c\u4f7f\u5305\u62ec\u7c92\u7dda\u9ad4\u5728\u5167\u7684\u7d30\u80de\u8cea\u767c\u51fa\u5f37\u70c8\u7684\u7da0\u8272\u87a2\u5149\u3002\u6dfb\u52a0CoCl2\u5f8c\uff0c\u7d30\u80de\u8cea\u7684\u87a2\u5149\u88abCoCl2\u731d\u6ec5\uff0c\u50c5\u5728\u7c92\u7dda\u9ad4\u7559\u4e0b\u87a2\u5149\u3002\u4f5c\u70ba\u5c0d\u7167\uff0c\u7d30\u80de\u53ef\u4ee5\u8ca0\u8f09Calcein AM\u548cCoCl2\uff0c\u4e26\u7528\u96e2\u5b50\u9ef4\u7d20\u8655\u7406\uff0c\u4f7f\u7d30\u80de\u8ca0\u8f09\u66f4\u591a\u7684Ca2+\uff0c\u9032\u800c\u5f15\u8d77MPTP\u7684\u6d3b\u5316\u548c\u7c92\u7dda\u9ad4\u87a2\u5149\u7684\u731d\u6ec5\u3002<\/p>\n

<\/p>\n

Fig. L929 cells was incubated with L929 cells Calcein AM (1X), and the cytoplasm including mitochondria emitted strong green fluorescence (A). After cells were further incubated with CoCl2 (1mM), the green fluorescence of Calcein in the cytoplasm was quenched by CoCl2, leaving only the green fluorescence in mitochondria (B). Cells treated with Ionomycin (0.25\u03bcM) induced a large amount of extracellular Ca2+ into the cell, excessive Ca2+ into the mitochondrial matrix, resulting in the opening of MPTP, partial release of Calcein from the mitochondria, and the introduction of cobalt ions into the mitochondria and green fluorescence quenching of Calcein (C).<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nMitochondrial Permeability Transition Pore Assay<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Calcein AM (1000\u00d7)
\n\u2022 CoCl2 (100\u00d7)
\n\u2022 Ionomycin (200\u00d7)
\n\u2022 Assay Buffer<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 High safety: almost no toxicity to cells;
\n\u2022 Compatibility with multiple detection methods: can be used in flow cytometry and fluorescence microscopy.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStored at -20\u2103 for 12 months, protected from light<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nMitochondrial permeablity transition pore (MPTP), also known as magachannel, is a non selective high conductivity channel that exists between the inner and outer membranes of mitochondria and is composed of multiple protein complexes. MPTP may be involved in the release of mitochondrial components during cell death. The normal mitochondrial inner membrane of a cell can maintain a normal mitochondrial potential gradient to ensure cellular respiration and energy supply. With the intake and release of Ca2+, a low conductivity permeability transition pore switches back and forth between opening and closing. During apoptosis and pathological death of cells, the permeability of mitochondrial membrane potential transfer pore changes, Ca2+ overload, mitochondrial glutathione oxidation, increase of reactive oxygen species, including subsequent release of cytochrome C, and mitochondrial membrane potential decline all lead to the activation of MPTP. Mitochondrial Permeability Transition Pore Assay Kit s a more direct detection method for detecting the opening of MPTP than only based on mitochondrial membrane potential analysis.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nMPTP<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

A. \u6d41\u5f0f\u7d30\u80de\u5100\u5206\u6790<\/span><\/strong><\/span>
\n1. \u7528\u6240\u9700\u65b9\u6cd5\u8655\u7406\u7d30\u80de\u3002<\/p>\n

2. \u5c0d\u65bc\u975e\u8cbc\u58c1\u7d30\u80de\uff0c\u6536\u96c61-5\u00d710\u4ee5\u96e2\u5fc3\uff08300 g\uff0c5 \u5206\u9418\uff09\u5206\u96e2 5 \u500b\u7d30\u80de\u3002\u7528 PBS \u6e05\u6d17\u5169\u6b21\u4e26\u4e1f\u68c4 PBS\u3002\u8cbc\u58c1\u7d30\u80de\uff0c\u5148\u7528\u80f0\u86cb\u767d\u9176\uff08\u4e0d\u542bEDTA\uff09\u6d88\u5316\u7d30\u80de\uff0c\u7136\u5f8c\u96e2\u5fc3\u3002\u6ce8\u610f\uff1a\u6211\u5011\u5efa\u8b70\u5c07\u672a\u67d3\u8272\u7684\u5c0d\u7167\u7d30\u80de\uff08\u5373\u672a\u67d3\u8272\uff09\u61f8\u6d6e\u5728\u7d93\u904e\u8655\u7406\u548c\u672a\u7d93\u8655\u7406\u7684\u6a23\u672c\u7684 1\u00d7\u6aa2\u6e2c\u7de9\u885d\u6db2\u4e2d\uff0c\u4ee5\u8a2d\u5b9a\u6d41\u5f0f\u7d30\u80de\u5100\u3002<\/p>\n

3. \u5206\u5225\u52a0\u5165\u9069\u91cf\u7684Calcein AM\u67d3\u8272\u6db2\u3001\u87a2\u5149\u6392\u968a\u6db2\u3001\u96e2\u5b50\u9ef4\u7d20\u5c0d\u7167\u6db2\uff0c\u91cd\u61f8\u7d30\u80de\u81f3\u7d30\u80de\u5bc6\u5ea6\u7d041\u00d7106<\/sup>\/\u6beb\u5347\u3002 37\u2103\u907f\u5149\u57f9\u990a30-45\u5206\u9418\uff0c\u4e0d\u540c\u7d30\u80de\u6709\u4e0d\u540c\u7684\u6700\u4f73\u57f9\u990a\u6642\u9593\u3002<\/p>\n

4. 300 g \u96e2\u5fc3 5 \u5206\u9418\u6536\u96c6\u7d30\u80de\u3002\u52a0\u51651mLAssay Buffer\uff0c\u8f15\u8f15\u91cd\u61f8\u7d30\u80de\uff0c300g\u96e2\u5fc35\u5206\u9418\u6536\u96c6\u7d30\u80de\u3002<\/p>\n

5. \u52a0\u5165400 \u03bcL Assay Buffer\u91cd\u61f8\u7d30\u80de\uff0c\u518d\u7528\u6d41\u5f0f\u7d30\u80de\u5100\u5206\u6790\u7d30\u80de<\/p>\n

B. \u87a2\u5149\u986f\u5fae\u93e1\u5206\u6790<\/strong><\/span>
\n1\uff0e\u5c0d\u65bc\u8cbc\u58c1\u7d30\u80de
\n(1) \u76f4\u63a5\u5728\u7d30\u80de\u57f9\u990a\u677f\u7684\u84cb\u73bb\u7247\u4e0a\u57f9\u990a\u7d30\u80de\u3002\u8655\u7406\u524d\u5728CO2\u57f9\u990a\u7bb1\u4e2d37\u2103\u5b75\u80b2\u81f3\u5c1124\u5c0f\u6642\u3002<\/p>\n

(2)\u7528\u6240\u9700\u65b9\u6cd5\u8655\u7406\u7d30\u80de\u3002\u900f\u904e\u5728\u6c92\u6709\u8a98\u5c0e\u5291\u7684\u60c5\u6cc1\u4e0b\u5b75\u80b2\u7d30\u200b\u200b\u80de\u4f86\u88fd\u5099\u9670\u6027\u5c0d\u7167\u3002<\/p>\n

(3)\u7528PBS\u6d17\u6ecc\u7d30\u80de\u5169\u6b21\u3002<\/p>\n

(4)\u5206\u5225\u5728\u7d30\u80de\u4e2d\u52a0\u5165\u9069\u91cf\u7684Calcein AM\u67d3\u8272\u6db2\u3001\u87a2\u5149\u6392\u968a\u6db2\u3001\u96e2\u5b50\u9ef4\u7d20\u5c0d\u7167\u6db2\u3002\u4e00\u822c\u60c5\u6cc1\u4e0b\uff0c96 \u5b54\u677f\u6bcf\u5b54\u52a0\u5165 100 \u03bcL\uff0c24 \u5b54\u677f\u6bcf\u5b54\u52a0\u5165 250 \u03bcL\uff0c12 \u5b54\u677f\u6bcf\u5b54\u52a0\u5165 500 \u03bcL\uff0c6 \u5b54\u677f\u6bcf\u5b54\u52a0\u5165 1 mL\u3002\u7136\u5f8c37\u2103\u5b75\u80b230-45\u5206\u9418\uff0c\u907f\u5149\uff0c\u4e0d\u540c\u7d30\u80de\u6709\u4e0d\u540c\u7684\u6700\u4f73\u57f9\u990a\u6642\u9593\u3002<\/p>\n

(5)\u5b75\u80b2\u7d50\u675f\u5f8c\uff0c\u4ee537\u2103\u9810\u71b1\u7684\u65b0\u9bae\u57f9\u990a\u57fa\u66ff\u63db\u67d3\u8272\u6db2\uff0c\u4e26\u572837\u2103\u907f\u5149\u5b75\u80b230min\uff0c\u4ee5\u78ba\u4fddCalcein AM\u88ab\u4e73\u7cd6\u9176\u5145\u5206\u6c34\u89e3\u6210\u5177\u6709\u7da0\u8272\u87a2\u5149\u7684Calcein\u3002<\/p>\n

(6)\u4ee5PBS\u6d17\u6ecc\u7d30\u80de2-3\u6b21\uff0c\u52a0\u5165Assay Buffer\u8986\u84cb\u7d30\u80de\uff0c\u7136\u5f8c\u5728\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u6a23\u672c(Calcein AM\u70ba\u7da0\u8272\u87a2\u5149\uff0cEx\/Em=494\/517nm)<\/p>\n

2\uff0e\u5c0d\u65bc\u975e\u8cbc\u58c1\u7d30\u80de<\/strong><\/span>
\n(1) \u7528\u6240\u9700\u7684\u65b9\u6cd5\u8655\u7406\u7d30\u80de\uff0c\u5c0d\u7d30\u80de\u9032\u884c\u8a08\u6578\u3002<\/p>\n

(2) 300 g \u96e2\u5fc3 5 min\uff0c\u6536\u96c6\u9069\u7576\u7d30\u80de\uff0c\u68c4\u4e0a\u6e05\uff0cPBS \u6d17\u6ecc\u7d30\u80de 2 \u6b21\uff0c\u68c4\u53bb PBS\u3002<\/p>\n

(3) \u5206\u5225\u52a0\u5165\u9069\u91cf\u7684\u9223\u9ec3\u7da0\u7d20AM\u67d3\u8272\u6db2\u3001\u87a2\u5149\u6392\u968a\u6db2\u3001\u96e2\u5b50\u9ef4\u7d20\u5c0d\u7167\u6db2\uff0c\u91cd\u61f8\u7d30\u80de\u81f3\u7d30\u80de\u5bc6\u5ea6\u7d041\u00d7106<\/sup>\/\u6beb\u5347\u3002 37\u2103\u907f\u5149\u57f9\u990a30-45\u5206\u9418\uff0c\u4e0d\u540c\u7d30\u80de\u6709\u4e0d\u540c\u7684\u6700\u4f73\u57f9\u990a\u6642\u9593\u3002<\/p>\n

(4) 300 g\u96e2\u5fc35 min\uff0c\u68c4\u4e0a\u6e05\uff0c\u7de9\u6162\u52a0\u51651 mL 37\u2103\u9810\u71b1\u7684\u57f9\u990a\u57fa\u91cd\u61f8\u7d30\u80de\uff0c37\u2103\u907f\u5149\u5b75\u80b230 min\uff0c\u78ba\u4fddCalcein AM\u88ab\u4e73\u7cd6\u9176\u5145\u5206\u6c34\u89e3\u6210Calcein\uff0c\u4e26\u767c\u51fa\u7da0\u8272\u87a2\u5149\u3002<\/p>\n

(5) 300 g \u96e2\u5fc35 min\uff0c\u68c4\u53bb\u5927\u90e8\u5206\u57f9\u990a\u57fa\uff0c\u5c07\u7d30\u80de\u91cd\u61f8\uff0c\u6ef4\u65bc\u73bb\u7247\u4e0a\uff0c\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u6a23\u672c\u3002<\/p>\n

\u5e38\u898b\u554f\u984c\u89e3\u7b54(Q&A)<\/span><\/strong><\/span><\/p>\n

Q\uff1a\u8a72\u8a66\u5291\u76d2\u76ee\u524d\u6aa2\u6e2c\u7684\u6a23\u672c\u985e\u578b\u6709\u54ea\u4e9b\uff1f<\/span><\/p>\n

A\uff1a\u672c\u8a66\u5291\u76d2\u53ef\u4ee5\u5c0d\u8cbc\u58c1\u7d30\u80de\u548c\u61f8\u6d6e\u7d30\u80de\u9032\u884c\u6aa2\u6e2c\uff0c\u5305\u62ec\uff1aL929 Jurkat\u7b49\u7d30\u80de\u3002<\/p>\n

Q\uff1a\u7d44\u7e54\u6216\u7c92\u7dda\u9ad4\u6a23\u54c1\u53ef\u4ee5\u6aa2\u6e2c\u55ce\uff1f<\/span><\/p>\n

A\uff1a\u7d44\u7e54\u7684\u55ae\u7d30\u80de\u61f8\u6db2(Single-cell suspension)\u53ef\u4ee5\u5617\u8a66\u3002\u63d0\u53d6\u51fa\u4f86\u7684\u7c92\u7dda\u9ad4\u5247\u4e0d\u9069\u5408\u3002<\/p>\n

Q\uff1a\u52a0\u5165\u6c2f\u5316\u9237(cobalt chloride)\u5f8c\uff0c\u7d30\u80de\u8cea\u5167\u7684\u87a2\u5149\u4e0d\u6dec\u6ec5\u600e\u9ebc\u8fa6\u5462<\/span><\/p>\n

A\uff1a\u53ef\u9069\u7576\u6e1b\u5c11Calcein-AM\u7684\u6fc3\u5ea6\u548c\u7528\u91cf\u3002<\/p>\n

Q\uff1a\u6700\u5f8c\u7684\u7d50\u679c\u61c9\u8a72\u5982\u4f55\u5206\u6790\uff1f\u4e0d\u540c\u5b50\u7fa4(subgroups)\u9593\u61c9\u8a72\u5982\u4f55\u6bd4\u8f03\uff1f<\/span><\/p>\n

A\uff1a\u64da\u7c92\u7dda\u9ad4\u4e2dCalcein\u7da0\u8272\u87a2\u5149\u7684\u5f37\u5f31\u4f86\u5224\u65b7\u7c92\u7dda\u9ad4MPTP\u958b\u653e\u7684\u7a0b\u5ea6\uff0c\u7da0\u8272\u87a2\u5149\u8d8a\u5f37\uff0c\u958b\u653e\u7a0b\u5ea6\u8d8a\u4f4e\uff0c\u7da0\u8272\u87a2\u5149\u8d8a\u5f31\u958b\u653e\u7a0b\u5ea6\u8d8a\u9ad8\u3002<\/p>\n

Q\uff1a\u8a72\u8a66\u5291\u76d2\u5982\u4f55\u6aa2\u6e2cMPTP\u958b\u653e\u7a0b\u5ea6\uff1f<\/span><\/p>\n

A\uff1a\u672c\u65b9\u6cd5\u76f8\u8f03\u57fa\u65bc\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u7684\u65b9\u6cd5\u80fd\u66f4\u76f4\u63a5\u5730\u6aa2\u6e2c\u5230\u7c92\u7dda\u9ad4\u901a\u900f\u6027\u8f49\u63db\u5b54\u958b\u653e\u7a0b\u5ea6\u7684\u8b8a\u5316\uff0c\u6839\u64da\u7c92\u7dda\u9ad4\u4e2dCalcein\u7da0\u8272\u87a2\u5149\u7684\u5f37\u5f31\u4f86\u5224\u65b7\u7c92\u7dda\u9ad4MPTP\u958b\u653e\u7684\u7a0b\u5ea6\uff0c\u7da0\u8272\u87a2\u5149\u8d8a\u5f37\uff0c\u958b\u653e\u7a0b\u5ea6\u8d8a\u4f4e\uff0c\u7da0\u8272\u87a2\u5149\u8d8a\u5f31\u958b\u653e\u7a0b\u5ea6\u8d8a\u9ad8\u3002\u5be6\u9a57\u9700\u8981\u8a2d\u7f6e\u9670\u6027\u5c0d\u7167\u548c\u967d\u6027\u5c0d\u7167\u9032\u884c\u6bd4\u8f03\uff0c\u6c92\u6709\u9748\u654f\u5ea6\u548c\u6aa2\u6e2c\u6975\u9650\u6578\u64da\uff0c\u87a2\u5149\u986f\u5fae\u93e1\u3001\u6d41\u5f0f\u5747\u53ef\u6aa2\u6e2c\u3002<\/p>\n

Q\uff1a\u8a72\u8a66\u5291\u76d2\u7684\u9670\u6027\u5c0d\u7167\u548c\u967d\u6027\u5c0d\u7167\u8a72\u5982\u4f55\u8a2d\u7f6e\uff1f<\/span><\/p>\n

A\uff1a\u9670\u6027\u6a23\u672c\u4e2d\u52a0\u5165Calcein AM staining solution\uff1b\u967d\u6027\u6a23\u672c\u4e2d\u52a0\u5165Ionomycin control\u3002<\/p>\n

Q\uff1a\u8a72\u8a66\u5291\u76d2\u67d3\u8272\u4e4b\u5f8c\u662f\u5426\u53ef\u4ee5\u7528DAPI\u67d3\u7d30\u80de\u6838\u5462\uff1f<\/span><\/p>\n

A\uff1a\u5982\u679c\u67d3\u6838\u63a8\u85a6Abbkine\u7684Hoechst 33342\u6d3b\u7d30\u80de\u67d3\u6599(BMD0062<\/a>)\u3002\u4e0d\u53ef\u56fa\u5b9a\u3002<\/p>\n

Q\uff1a\u8a72\u8a66\u5291\u76d2\u61c9\u8a72\u9078\u7528\u54ea\u500b\u901a\u9053(channel)\u9032\u884c\u6aa2\u6e2c\uff1f<\/span><\/p>\n

A\uff1a\u9078\u7528FITC\/488 nm \u6fc0\u767c\u5149\u901a\u9053\u9032\u884c\u6aa2\u6e2c\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u7c92\u7dda\u9ad4\u6ef2\u900f\u6027Transition Pore\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTA4002 (Ex\/Em=494\/517 n […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[6366],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/47141"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=47141"}],"version-history":[{"count":9,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/47141\/revisions"}],"predecessor-version":[{"id":47159,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/47141\/revisions\/47159"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=47141"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=47141"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=47141"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}