{"id":45908,"date":"2023-10-02T16:26:18","date_gmt":"2023-10-02T08:26:18","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45908"},"modified":"2023-10-22T12:05:56","modified_gmt":"2023-10-22T04:05:56","slug":"%e5%b1%b1%e6%a2%a8%e9%86%87%e8%84%ab%e6%b0%ab%e9%85%b6%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e6%af%94%e8%89%b2%e6%b3%95%ef%bc%89-sorbitol-dehydrogenase-assay-kit-colorimetric","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=45908","title":{"rendered":"\u5c71\u68a8\u9187\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09| Sorbitol Dehydrogenase Assay Kit (Colorimetric) \u8ca8\u865fAbnova\u2122 KA4567"},"content":{"rendered":"

\u5c71\u68a8\u9187\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09| Sorbitol Dehydrogenase Assay Kit (Colorimetric) \u8ca8\u865fAbnova\u2122 KA4567<\/a><\/strong><\/span><\/p>\n

Sorbitol Dehydrogenase Assay Kit (Colorimetric)-0.1 to 125 U\/L<\/p>\n

<\/p>\n

100 assays\u00a0<\/span><\/p>\n

\u5c71\u68a8\u9187\u812b\u6c2b\u9176 (SORBITOL DEHYDROGENASE, SDH) \u662f\u4e00\u7a2e\u50ac\u5316\u5c71\u68a8\u9187(sorbitol)\u548c\u679c\u7cd6(fructose)\u3002\u8840\u6e05 SDH (blood serum SDH )\u6c34\u6e96\u5347\u9ad8\u8868\u793a\u809d\u640d\u50b7(liver damage)\uff1b\u56e0\u6b64\uff0c SDH (blood serum SDH )\u5728\u809d\u81df\u75be\u75c5\u7684\u8a3a\u65b7\uff0c\u7279\u5225\u662f\u8207\u8f49\u6c28\u9176(aminotransferases)\u7d50\u5408\u4f7f\u7528\u3002 \u5c71\u68a8\u9187\u812b\u6c2b\u9176 (SORBITOL DEHYDROGENASE, SDH) (blood serum SDH ) \u6c34\u5e73\u4e5f\u88ab\u6e2c\u91cf\u70ba\u8a55\u4f30\u7cd6\u5c3f\u75c5\u4f75\u767c\u75c7(diabetic complications )\uff0c\u4f8b\u5982\u589e\u6b96\u6027\u7cd6\u5c3f\u75c5\u8996\u7db2\u819c\u75c5\u8b8a(proliferative diabetic retinopathy)\u3002\u9019\u7a2e\u975e\u653e\u5c04\u6027\u6bd4\u8272 SDH (blood serum SDH ) \u6e2c\u5b9a\u662f\u57fa\u65bc\u56db\u5511\u9e7d MTT \u7684\u9084\u539fNADH \u5076\u806f\u9176\u4fc3\u53cd\u61c9\u8207 MTT \u7684\u9084\u539f\u5f62\u5f0f\u53cd\u61c9\uff0c\u5728 565 nm \u8655\u5448\u73fe\u6700\u5927\u5438\u6536\u3002565 nm \u8655\u5438\u5149\u5ea6\u7684\u589e\u52a0\u8207\u9175\u7d20\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/p>\n

<\/p>\n

\u61c9\u7528\uff1a <\/span><\/strong><\/span><\/p>\n

\u2713 \u751f\u7269\u6a23\u672c\uff08\u4f8b\u5982\u8840\u6f3f\u3001\u8840\u6e05\u3001\u5c3f\u6db2\u3001\u7d44\u7e54\u548c\u57f9\u990a\u57fa\uff09\u4e2d SDH \u6d3b\u6027\u6e2c\u5b9a\u3002<\/p>\n

\u7279\u9ede\uff1a <\/span><\/strong><\/span><\/p>\n

\u2713 \u5feb\u901f\u3001\u9748\u654f\u3002\u7dda\u6027\u6aa2\u6e2c\u7bc4\u570d\uff0820 \u00b5L \u6a23\u54c1\uff09\uff1a0.1 \u81f3 125 U\/L\uff0c\u53cd\u61c9 12 \u5206\u9418\u3002<\/p>\n

\u2713 \u65b9\u4fbf\u3001\u9ad8\u901a\u91cf\u3002\u5747\u8cea\u300c\u6df7\u5408-\u5b75\u80b2-\u6e2c\u91cf\u300d\u578b\u6e2c\u5b9a\u3002\u53ef\u5728 HTS \u6db2\u9ad4\u8655\u7406\u7cfb\u7d71\u4e0a\u8f15\u9b06\u81ea\u52d5\u5316\uff0c\u6bcf\u5929\u8655\u7406\u6578\u5343\u500b\u6a23\u54c1\u3002<\/p>\n

Summary<\/span><\/strong><\/span><\/p>\n

\u2713 \u7d44\u7e54\uff1a\u89e3\u5256\u524d\uff0c\u7528\u78f7\u9178\u9e7d\u7de9\u885d\u6db2\uff08PBS\uff0cpH 7.4\uff09\u6c96\u6d17\u7d44\u7e54\u4ee5\u53bb\u9664\u8840\u6db2\u3002\u5728 200 \u00b5L \u51b7 PBS \u7de9\u885d\u6db2\u4e2d\u5747\u8cea\u7d44\u7e54\uff0850 mg\uff09\u3002\u5728 4\u00b0 C \u4e0b\u4ee514,000 x g \u96e2\u5fc35 \u5206\u9418\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\u3002<\/p>\n

\u2713 \u7d30\u80de\u9593\u9699\u6db2\uff1a\u57284\u00b0C \u4e0b\u4ee5 2,000 x g \u96e2\u5fc35 \u5206\u9418\u6536\u96c6\u7d30\u80de\u3002\u5c0d\u65bc\u58c1\u8cbc\u7d30\u80de\uff0c\u4e0d\u8981\u4f7f\u7528\u86cb\u767d\u6c34\u89e3\u9176\u6536\u7a6b\u7d30\u80de\uff1b\u76f8\u53cd\u5730\u4f7f\u7528\u6a61\u81a0\u8b66\u5bdf\u3002\u4ee5\u9069\u7576\u9ad4\u7a4d\u7684\u51b7PBS \u7de9\u885d\u6db2\u4e2d\u52fb\u6f3f\u6216\u97f3\u6ce2\u8655\u7406\u7d30\u80de\u3002\u5728\u8d85\u904e4\u00b0C \u4e0b\u4ee514,000 x g \u96e2\u5fc35 \u5206\u9418\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\u3002<\/p>\n

\u2713 \u6240\u6709\u6a23\u54c1\u5747\u53ef\u5728 \u201320 \u81f3 \u201380\u00b0C \u4e0b\u4fdd\u5b58\u81f3\u5c11 1 \u500b\u6708\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

<\/p>\n

\n

Product Description<\/span><\/h4>\n

Sorbitol Dehydrogenase Assay Kit (Colorimetric) is used for quantitative determination of Sorbitol Dehydrogenase activity using colourmetric methods.<\/p>\n<\/div>\n

\n

Suitable Sample<\/span><\/strong><\/span><\/h4>\n

Culture media, Plasma, Serum, Tissue, Urine<\/p>\n<\/div>\n

\n

Sample Volume<\/span><\/strong><\/span><\/h4>\n

20 uL<\/p>\n<\/div>\n

\n

Detection Method<\/span><\/strong><\/span><\/h4>\n

Colorimetric<\/p>\n<\/div>\n

\n

Assay Type<\/span><\/strong><\/span><\/h4>\n

Quantitative<\/p>\n<\/div>\n

\n

Calibration Range<\/span><\/strong><\/span><\/h4>\n

0.1 to 125 U\/L<\/p>\n<\/div>\n

\n

Regulation Status<\/span><\/strong><\/span><\/h4>\n

For research use only (RUO)<\/p>\n<\/div>\n

\n

Quality Control Testing<\/span><\/strong><\/span><\/h4>\n

Standard Curve<\/p>\n

The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.<\/p>\n<\/div>\n

\n

Storage Instruction<\/span><\/h4>\n

Store the kit at -20\u00b0C.<\/p>\n

References<\/span><\/strong><\/p>\n<\/div>\n

1. Uzozie, A et al (2014). Sorbitol Dehydrogenase overexpression and other aspects of dysregulated
\nprotein expression in human precancerous colorectal neoplasms: a quantitative proteomics study. Mol
\nCell Proteomics. 13(5):1198-1218.
\n2. Linstad, RI at al (2013). Inhibition of Sorbitol Dehydrogenase by nucleosides and nucleotides. Biochem
\nBiophys Res Commun. 435(2): 202-208.
\n3. Aguayo, MF et al (2013). Sorbitol dehyodrogenase is a cytosolic protein required for sorbitol metabolism
\nin Arabidopsis thalania. Plant Sci. 205-206: 63-75.<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5c71\u68a8\u9187\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09| Sorbitol Dehydrogenase Assay Kit (Col […]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6127],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45908"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45908"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45908\/revisions"}],"predecessor-version":[{"id":45915,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45908\/revisions\/45915"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45908"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45908"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45908"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}