{"id":45355,"date":"2023-08-24T14:21:49","date_gmt":"2023-08-24T06:21:49","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45355"},"modified":"2023-08-28T17:18:22","modified_gmt":"2023-08-28T09:18:22","slug":"minute-%e6%8a%97%e7%b5%90%e5%a1%8a%e7%b4%b0%e8%83%9e%e6%a0%b8%e5%84%b2%e5%ad%98%e7%b7%a9%e8%a1%9d%e6%b6%b2anti-clumping-nuclei-storage-buffer-invent%e8%b2%a8%e8%99%9fwa-014-2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=45355","title":{"rendered":"Minute\u2122 \u6297\u7d50\u584a\u7d30\u80de\u6838\u5132\u5b58\u7de9\u885d\u6db2| Anti-Clumping Nuclei Storage Buffer Invent\u8ca8\u865fWA-014"},"content":{"rendered":"<p><strong><span style=\"font-size: 14pt; color: #800000;\">\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-<span style=\"color: #ff9900;\">Rapid Protein Extraction Kits<\/span><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-minute_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1.jpg\" \/><\/p>\n<p><strong>Minute\u2122<\/strong><strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300eColumn Filter\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong><strong>\u00a0<\/strong><strong>\u4e00 \u5206\u9418<\/strong><strong>\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<em>vortex\u3001transfer\u3001spin \u3002<\/em><\/strong>\u907f\u514d RIPA \u4e0d\u6eb6\u6027\u86cb\u767d\u8cea\u7684\u554f\u984c\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u65b9\u6cd5\u7372\u5f97\u66f4\u5b8c\u6574\u7684\u86cb\u767d\u8cea\u8b5c\u3002Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n<p><iframe loading=\"lazy\" title=\"Minute Total Protein Extraction Kit - Invent Biotechnologies\" src=\"\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" width=\"562\" height=\"316\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/p>\n<p><a href=\"https:\/\/biopioneer.com.tw\/?p=45355&amp;preview=true\"><strong><span style=\"color: #33cccc; font-size: 12pt;\">Minute\u2122 \u6297\u7d50\u584a\u7d30\u80de\u6838\u5132\u5b58\u7de9\u885d\u6db2| Invent\u8ca8\u865fWA-014<\/span><\/strong><\/a><\/p>\n<p><span style=\"color: #999999; font-size: 12pt;\">Minute\u2122 Anti-Clumping Nuclei Storage Buffer (10 ml)<\/span><\/p>\n<p><strong><span style=\"font-size: 12pt;\"><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-014_v1.pdf?v=1633973539\" target=\"_blank\" rel=\"noopener\">\u624b\u518a\u548c\u5354\u8b70<\/a><\/span>\u00a0|\u00a0<span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-8_edbbb1d9-57b5-4450-b6fd-bc6ee6e95ace.pdf?v=1633969533\" target=\"_blank\" rel=\"noopener\">\u6750\u6599\u5b89\u5168\u6578\u64da\u8868 (MSDS)<\/a>\u00a0 \u00a0<\/span> \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/span> \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<\/strong><\/p>\n<p>\u5f9e\u52d5\u7269\u7d30\u80de\/\u7d44\u7e54\u4e2d\u5206\u96e2\u7d30\u80de\u6838( nuclei isolation)\u7684\u50b3\u7d71\u65b9\u6cd5\u4f9d\u8cf4\u65bc\u4f7f\u7528\u975e\u96e2\u5b50\u53bb\u6c61\u5291(non-ionic detergent)\u88c2\u89e3\u8cea\u819c( plasma membrane)\u4e26\u91cb\u653e\u7d30\u80de\u6838\uff0c\u96a8\u5f8c\u53ef\u4ee5\u901a\u904e\u4f4e\u901f\u96e2\u5fc3\u5206\u96e2(low-speed centrifugation)\u4e26\u901a\u904e\u5bc6\u5ea6\u68af\u5ea6(density gradient)\u9032\u4e00\u6b65\u7d14\u5316\u3002\u8207\u50b3\u7d71\u65b9\u6cd5\u76f8\u95dc\u7684\u4e00\u500b\u4e3b\u8981\u554f\u984c\u662f\u5206\u96e2\u5f8c\u7d30\u80de\u6838\u7684\u805a\u96c6(clumping)\uff0c\u9019\u5728 DNA \u548c RNA \u63d0\u53d6\u7b49\u67d0\u4e9b\u61c9\u7528\u4e2d\u53ef\u80fd\u4e0d\u662f\u554f\u984c\u3002\u7136\u800c\uff0c\u5728\u55ae\u6838\u6e2c\u5e8f(single nucleus sequencing)\u6216\u86cb\u767d\u8cea\u7d44\u5b78(proteomics)\u7b49\u5176\u4ed6\u61c9\u7528\u4e2d\uff0c\u5206\u96e2\u6838\u7684\u805a\u96c6\u662f\u4e00\u500b\u4e3b\u8981\u554f\u984c\u3002\u6297\u7d50\u584a\u6838\u5b58\u5132\u7de9\u885d\u6db2(anti-clumping nuclei storage buffer)\u65e8\u5728\u89e3\u6c7a\u9019\u500b\u554f\u984c\u3002\u8207\u91cd\u61f8\u65bc\u542b 5% BSA \u7684 PBS \u4e2d\u7684\u7d30\u80de\u6838\u76f8\u6bd4\uff0c\u91cd\u61f8\u65bc\u8a72\u7de9\u885d\u6db2\u4e2d\u7684\u7d30\u80de\u6838\u986f\u793a\u51fa\u986f\u8457\u6e1b\u5c11\u7684\u7d50\u584a\u3002\u7d30\u80de\u6838\u61f8\u6db2\u53ef\u4fdd\u5b58\u65bc4 <sup>\u2103<\/sup> \u6578\u5929\uff0c\u6c92\u6709\u660e\u986f\u7684\u805a\u96c6( aggregation)\u548c\u5f62\u614b( morphology)\u8b8a\u5316\u3002\u7d30\u80de\u6838\u4e5f\u53ef\u4ee5\u51b7\u51cd\u5728 -80 <sup>o<\/sup>\u00a0C \u7684\u7de9\u885d\u6db2\u4e2d\u6216\u4e7e\u51b0\u4e2d\uff0c\u4ee5\u4fbf\u904b\u8f38\u6216\u9577\u671f\u5132\u5b58\u3002\u5c0d\u65bc\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u55ae\u6838\u5206\u96e2\uff0c\u00a0<a href=\"https:\/\/inventbiotech.com\/collections\/single-nuclei-isolation\" target=\"_blank\" rel=\"noopener\">click here<\/a>\u67e5\u770b\u53ef\u7528\u7522\u54c1\u5217\u8868\u3002<\/p>\n<p>For spin column-based single nuclei isolation,\u00a0<a href=\"https:\/\/inventbiotech.com\/collections\/single-nuclei-isolation\" target=\"_blank\" rel=\"noopener\">click here<\/a>\u00a0for the\u00a0list of available products.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E6%8A%97%E7%B5%90%E5%A1%8A%E7%B4%B0%E8%83%9E%E6%A0%B8%E5%84%B2%E5%AD%98%E7%B7%A9%E8%A1%9D%E6%B6%B2-Invent%E8%B2%A8%E8%99%9FWA-014-2-1.webp\" \/><\/p>\n<p><strong>\u5b58\u5132\u7de9\u885d\u6db2\u8207 PBS \u4e2d 5% BSA \u7684\u6297\u7d50\u584a\u529f\u6548\u6bd4\u8f03\u3002<\/strong>\u901a\u904e\u7d44\u7e54\/\u7d30\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2\uff08\u76ee\u9304\u865fSN-047\uff0cInvent Biotechnologies\uff0c\u660e\u5c3c\u8607\u9054\u5dde\u666e\u5229\u8305\u65af\uff09\u5206\u96e2\u51b7\u51cd\u5c0f\u9f20\u813e\u81df\u548c\u80ba\u7d44\u7e54\u7684\u7d30\u80de\u6838\u3002\u5c07\u5206\u96e2\u7684\u7d30\u80de\u6838\u6c89\u6fb1\u91cd\u61f8\u65bc 0.4 ml \u7d30\u80de\u6838\u5132\u5b58\u7de9\u885d\u6db2\u6216 0.4 ml 5% BSA \u7684 PBS \u6eb6\u6db2\u4e2d\uff0c\u4e26\u5728 4\u2103 \u4e0b\u5b75\u80b2<sup>\u904e\u591c<\/sup>\u3002\u901a\u904e\u77ed\u66ab\u6e26\u65cb\u91cd\u61f8\u7d30\u80de\u6838\u4e26\u7528\u53f0\u76fc\u85cd\u67d3\u8272\u4ee5\u6bd4\u8f03\u7d30\u80de\u6838\u5f62\u614b\u3002<\/p>\n<p><strong>Comparison of the Storage Buffer with 5% BSA in PBS for Anti-Clumping Efficacy.<\/strong>\u00a0Nuclei of frozen mouse spleen and lung tissues were isolated by Nucleus Isolation Kit for Tissues\/cells (Cat# SN-047, Invent Biotechnologies, Plymouth MN). The isolated nuclei pellets were resuspended in 0.4 ml nuclei storage buffer or 0.4 ml 5% BSA in PBS and incubated at 4<sup>o<\/sup>C overnight. \u00a0The nuclei were resuspended by vortexing briefly and stained with trypan blue to compare the nuclear morphologies.<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Minute\u2122 \u6297\u7d50\u584a\u7d30\u80de\u6838\u5132\u5b58\u7de9\u885d\u6db2Kit includes:<\/strong><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E6%8A%97%E7%B5%90%E5%A1%8A%E7%B4%B0%E8%83%9E%E6%A0%B8%E5%84%B2%E5%AD%98%E7%B7%A9%E8%A1%9D%E6%B6%B2-Invent%E8%B2%A8%E8%99%9FWA-014-1.webp\" width=\"354\" height=\"354\" \/><\/p>\n<p>Resuspend isolated nuclear pellet in the buffer by gently pipetting up and down for 15-20 times. Generally, use 200-500 \uf06dl of storage buffer for 1-5 million of nuclei. Resupended nuclei can be stored at 4<sup>o<\/sup>C for up to one week or frozen at -80<sup>o<\/sup>C for up to 12 months without significant change in morphologies. It is important to note that this buffer can reduce well separated nucleus from clumping but has limited efficacy to separate nuclei that have already clumped. It is recommended to use Minute<sup>TM<\/sup> Single Nuclei Isolation Kits (Cat#: BN-020, NI-024, AN-029 or SN-047) to obtain well separated nuclei to start with. Nuclei in the storage buffer can be collected by centrifuging at 600 X g for 5 min at 4oC followed by resuspending in buffer(s) suitable for downstream application(s).<\/p>\n<p><strong><a href=\"https:\/\/inventbiotech.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%8E%9F%E5%BB%A0%E9%80%A3%E7%B5%9001-300x79.png\" alt=\"\u539f\u5ee0\u9023\u7d5001\" width=\"138\" height=\"36\" \/><\/a><\/strong><strong><a href=\"https:\/\/www.abmgood.com\/\"><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/a><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/strong><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u57fa\u65bc\u65cb\u8f49\u67f1(SPIN-COLUMN-BASED )\u7684\u86cb\u767d\u8cea\u63d0\u53d6\/\u7d30\u80de\u5206\u7d1a(CELL FRACTIONATION)<\/span><\/strong><\/span><\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-4356\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2015\/02\/Compined-F.gif\" alt=\"Compined-F\" width=\"446\" height=\"167\" \/><\/strong><\/p>\n<p>Invent Biotechnologies \u6210\u7acb\u65bc 2010 \u5e74\uff0c\u56e0\u9769\u65b0\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u800c\u4eab\u8b7d\u5168\u7403\u3002\u6211\u5011\u958b\u767c\u4e86\u57fa\u65bc\u65cb\u8f49\u67f1(spin column-based)\u7684\u5275\u65b0\u6280\u8853\uff0c\u7528\u65bc\u86cb\u767d\u8cea\u7814\u7a76( protein research)\uff0c\u53ef\u7c21\u5316\u548c\u589e\u5f37\u6a23\u54c1\u88fd\u5099\u3002\u9019\u6703\u5e36\u4f86\u66f4\u9806\u66a2\u7684\u5de5\u4f5c\u6d41\u7a0b\u548c\u59cb\u7d42\u53ef\u9760\u7684\u7d50\u679c\uff0c\u5f9e\u800c\u5e36\u4f86\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002\u9078\u64c7 Minute\u2122 \u8a66\u5291\u76d2\u9032\u884c\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2(cell fractionation)\u7684\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u76f8\u4fe1\uff0c\u4ed6\u5011\u7684\u6a23\u54c1\u6e96\u78ba\u5730\u4ee3\u8868\u4e86\u6240\u7814\u7a76\u7684\u7d30\u80de\u6216\u7d44\u7e54\u7684\u81ea\u7136\u7279\u5fb5\u3002\u9019\u4e9b\u5957\u4ef6\u53ea\u9700\u8981\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\uff0c\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u5be6\u9a57\u8b8a\u91cf\u4e26\u78ba\u4fdd\u9ad8\u8cea\u91cf\u7684\u6750\u6599\u4ee5\u7372\u5f97\u66f4\u53ef\u9760\u7684\u6578\u64da\u3002Minute\u2122 \u65b9\u6cd5\u7684\u6709\u6548\u6027\u5f97\u5230\u4e86\u8d8a\u4f86\u8d8a\u591a journals\u7684\u652f\u6301 (Nature, Science, and Cell.)\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #ff0000;\"><b>The Minute\u2122 \u7684\u512a\u9ede\uff1a<\/b><\/span><br \/>\n\u2022 \u8d85\u5feb\uff08\u5feb\u81f3 1 \u5206\u9418\uff09<br \/>\n\u2022 \u6a23\u672c\u91cf\u5c0f<br \/>\n\u2022 \u6700\u5c0f\u5316\u4ea4\u53c9\u6c61\u67d3<br \/>\n\u2022 \u5167\u6e90\u6027\u57fa\u7dda\u4e0d\u8b8a<br \/>\n\u2022 \u6240\u6709\u6d3b\u9ad4\u548c\u7d44\u7e54\u985e\u578b<\/p>\n<p><span style=\"color: #ff0000;\"><strong><span style=\"font-size: 12pt;\">The Minute\u2122 \u61c9\u7528\uff1a<\/span><\/strong><\/span><br \/>\n\u2022 \u86cb\u767d\u8cea\u904b\u8f38\/\u6613\u4f4d<br \/>\n\u2022 \u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528<br \/>\n\u2022 \u86cb\u767d\u8cea\u4fee\u98fe<br \/>\n\u2022 \u4fe1\u865f\u8f49\u5c0e<br \/>\n\u2022 sc\/snRNA-seq<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">Invent Minute\u2122 \u5fb9\u5e95\u6539\u8b8a\u86cb\u767d\u8cea\u6a23\u54c1\u88fd\u5099\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2<\/span><\/strong><\/p>\n<div class=\"rte\">\n<p>\u81ea 2010 \u5e74\u6210\u7acb\u4ee5\u4f86\uff0c\u6211\u5011\u7684\u9996\u8981\u76ee\u6a19\u662f\u5229\u7528\u6211\u5011\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u5275\u65b0\u6280\u8853\u5fb9\u5e95\u6539\u8b8a\u5168\u7403\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u5e02\u5834\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u8b93 Minute\u2122 \u8a66\u5291\u76d2\u6210\u70ba\u6a19\u6e96\u5be6\u9a57\u5ba4\u7a0b\u5e8f\uff0c\u53d6\u4ee3\u6240\u6709\u57fa\u65bc\u6eb6\u6db2\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u3002\u901a\u904e\u5229\u7528\u9019\u4e9b\u5957\u4ef6\uff0c\u7814\u7a76\u4eba\u54e1\u53ea\u9700\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\u5373\u53ef\u7372\u5f97\u4e00\u81f4\u7684\u7d50\u679c\u4e26\u7372\u5f97\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/5-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"491\" height=\"239\" \/><\/p>\n<p><span style=\"font-size: 12pt;\"><strong><span style=\"color: #800000;\">\u6539\u8b8a\u86cb\u767d\u8cea\u63d0\u53d6(Transforming protein extraction)<\/span><\/strong><\/span><\/p>\n<p>Minute\u2122 \u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1( simple spin column )\uff0c\u53ef\u6709\u6548\u6d88\u9664\u7d30\u80de\u6216\u7d44\u7e54\u88c2\u89e3\u904e\u7a0b(lysis)\u4e2d\u7684\u975e\u7cfb\u7d71\u6027\u86cb\u767d\u8cea\u640d\u5931(non-systematic protein loss)\u3002\u9019\u4ee3\u8868\u8457\u76f8\u5c0d\u65bc\u50b3\u7d71\u7684\u57fa\u65bc\u89e3\u6c7a\u65b9\u6848\u7684\u65b9\u6cd5\uff0c\u4f8b\u5982 RIPA \u7de9\u885d\u6db2(RIPA buffer)\u548c\u8d85\u8072\u8655\u7406(sonication)\u7684\u986f\u8457\u9032\u6b65\uff0c\u9019\u4e9b\u65b9\u6cd5\u4ee5\u5176\u53ef\u8b8a\u6027\u548c\u7121\u6cd5\u63d0\u4f9b\u5168\u9762\u7684\u86cb\u767d\u8cea\u8b5c\u800c\u805e\u540d\u3002\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\u7684 Minute\u2122 \u8a66\u5291\u76d2\u8207\u4f86\u81ea\u5404\u7a2e\u751f\u7269\u9ad4\u548c\u7d44\u7e54\u985e\u578b\u7684\u5c0f\u6a23\u672c\u91cf\u517c\u5bb9\uff0c\u70ba\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u4e86\u672a\u6539\u8b8a\u7684\u5167\u6e90\u86cb\u767d\u57fa\u7dda( endogenous proteins)\uff0c\u5f9e\u800c\u5be6\u73fe\u66f4\u7cbe\u78ba\u7684\u5b9a\u91cf\u548c\u5206\u6790\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-Biotechs-Minute-Kit-shows-a-more-complete-cell-lysis-compared-to-RIPA-buffer-in-which-a-pellet-remains-leading-to-an-alteration-of-the-protein-profile.jpg\" width=\"374\" height=\"481\" \/><\/p>\n<p>Invent Biotech&#8217;s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/3-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"488\" height=\"243\" \/><\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">\u6539\u9032\u7d30\u80de\u5206\u7d1a\u5206\u96e2(Revamping cell fractionation)<\/span><\/strong><\/p>\n<p>\u7531\u65bc\u7f3a\u4e4f\u7c21\u55ae\u7684\u5be6\u9a57\u65b9\u6848\uff0c\u50b3\u7d71\u7684\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u65b9\u6cd5\u9577\u671f\u4ee5\u4f86\u4e00\u76f4\u53d7\u5230\u7528\u6236\u504f\u5dee\u548c\u7d50\u679c\u4e0d\u4e00\u81f4\u7684\u5f71\u97ff\u3002 Minute\u2122 \u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2\u5fb9\u5e95\u6539\u8b8a\u4e86\u9019\u4e00\u904e\u7a0b\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u5728 90 \u5206\u9418\u5167\u7372\u5f97\u9ad8\u7d14\u5ea6\u7684\u7d30\u80de\u5668\u7fa4\uff0c\u4e26\u4e14\u4f7f\u7528\u6700\u5c11\u7684\u6d17\u6ecc\u5291\u3002\u901a\u904e\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1\uff0c\u9019\u4e9b\u8a66\u5291\u76d2\u7121\u9700\u5747\u8cea\u5316(homogenization)\u3001\u5bc6\u5ea6\u68af\u5ea6\u5206\u96e2(density gradient separation)\u548c\u8d85\u901f\u96e2\u5fc3\u7b49\u50b3\u7d71\u6280\u8853( ultra-centrifugation)\uff0c\u5f9e\u800c\u6e1b\u5c11\u4e86\u5be6\u9a57\u8b8a\u91cf\u7684\u6578\u91cf\u3002\u9019\u53ef\u4ee5\u9632\u6b62\u7d30\u80de\u5668\u640d\u50b7( organelle damage)\u4e26\u7522\u751f\u66f4\u4e00\u81f4\u548c\u751f\u7406\u76f8\u95dc\u7684\u7d50\u679c\u3002\u6191\u85c9\u591a\u7a2e Minute\u2122 \u8a66\u5291\u76d2\u53ef\u6eff\u8db3\u4e0d\u540c\u7684\u7d30\u80de\u5206\u7d1a(cell fractionation)\u5206\u96e2\u8981\u6c42\uff0c\u6211\u5011\u6b63\u5728\u63a8\u52d5\u86cb\u767d\u8cea\u7d44\u7814\u7a76\u7684\u8f49\u578b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/2-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"480\" height=\"238\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u652f\u6301\u6240\u6709\u7814\u7a76\u5b78\u79d1(Supporting all research disciplines)<\/strong><\/span><\/p>\n<p>Minute\u2122 \u8a66\u5291\u76d2\u65e8\u5728\u7121\u7e2b\u96c6\u6210\u5230\u73fe\u6709\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u63d0\u4f9b\u5feb\u901f\u4e14\u4e00\u81f4\u7684\u6027\u80fd\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u7d50\u679c\u3002\u7136\u800c\uff0c\u4e0d\u8981\u53ea\u76f8\u4fe1\u6211\u5011\u7684\u8a71\u2014\u2014\u6211\u5011\u7684\u7522\u54c1\u5df2\u5728\u79d1\u5b78\u6587\u737b\u4e2d\u88ab\u5ee3\u6cdb\u5f15\u7528\uff0c\u9019\u8868\u660e\u7814\u7a76\u754c\u5c0d\u7c21\u5316\u4e14\u53ef\u9760\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u7684\u9700\u6c42\u4e0d\u65b7\u589e\u9577\u3002\u7acb\u5373\u5207\u63db\u5230 Minute\u2122\uff0c\u9ad4\u9a57\u6211\u5011\u6280\u8853\u7684\u512a\u52e2\uff0c\u7121\u8ad6\u60a8\u7684\u7814\u7a76\u9818\u57df\u5982\u4f55\u3002<\/p>\n<table style=\"height: 2673px;\" width=\"995\">\n<tbody>\n<tr>\n<td width=\"64\">SD-001\/SN-002<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-003<\/td>\n<td>Minute <sup>TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-006<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005-p<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MP-007<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-038<\/td>\n<td>Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SD-008\/SN-009<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-010<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CP-011<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SB-004<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-012<\/td>\n<td>Minute <sup>TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NE-013<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HP-014<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YT-015<\/td>\n<td width=\"667\">Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YD-016<\/td>\n<td width=\"667\">Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YM-017<\/td>\n<td>Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-018<\/td>\n<td width=\"667\">Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-019<\/td>\n<td width=\"667\">Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HD-021<\/td>\n<td width=\"667\">MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AT-022<\/td>\n<td width=\"667\">MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AF-023<\/td>\n<td>MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NI-024<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">BN-020<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-047<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AN-029<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">FE-025<\/td>\n<td>Minute<sup>TM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MS-026<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Mass Spectrometry (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">EI-027<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ED-028<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Endosome Isolation and Cell Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SE-030<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CS-031<\/td>\n<td>Minute<sup>TM<\/sup> Cell Suspension Isolation from Fresh\/Frozen Tissues (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NT-032<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NM-033<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LY-034<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-035<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> High Fidelity ER Isolation Kit for Liver Tissue (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-036<\/td>\n<td>Minute<sup>TM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">GO-037<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-039<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-042<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PT-040<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-041<\/td>\n<td>Minute<sup>TM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PF-045<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SV-043<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ML-044<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">RD-046<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Rubisco Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PR-048<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant ER Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PG-049<\/td>\n<td>Minute<sup>TM<\/sup> Plant Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PE-050<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Endosome Enrichment Kit (20 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