{"id":45194,"date":"2023-08-18T16:16:19","date_gmt":"2023-08-18T08:16:19","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45194"},"modified":"2023-10-22T12:39:48","modified_gmt":"2023-10-22T04:39:48","slug":"minute-%e9%81%8e%e6%b0%a7%e5%8c%96%e7%89%a9%e9%85%b6%e6%8a%91%e8%a3%bd%e5%8a%91peroxidase-suppressor-invent%e8%b2%a8%e8%99%9fwa-005","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=45194","title":{"rendered":"Minute\u2122 \u904e\u6c27\u5316\u7269\u9176\u6291\u88fd\u5291|Peroxidase Suppressor Invent\u8ca8\u865fWA-005"},"content":{"rendered":"<p><strong><span style=\"font-size: 14pt; color: #800000;\">\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-<span style=\"color: #ff9900;\">Rapid Protein Extraction Kits<\/span><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-minute_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1.jpg\" \/><\/p>\n<p><strong>Minute\u2122<\/strong><strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300eColumn Filter\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong><strong>\u00a0<\/strong><strong>\u4e00 \u5206\u9418<\/strong><strong>\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<em>vortex\u3001transfer\u3001spin \u3002<\/em><\/strong>\u907f\u514d RIPA \u4e0d\u6eb6\u6027\u86cb\u767d\u8cea\u7684\u554f\u984c\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u65b9\u6cd5\u7372\u5f97\u66f4\u5b8c\u6574\u7684\u86cb\u767d\u8cea\u8b5c\u3002Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n<p><iframe loading=\"lazy\" title=\"Minute Total Protein Extraction Kit - Invent Biotechnologies\" src=\"\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" width=\"562\" height=\"316\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/p>\n<p><a href=\"https:\/\/biopioneer.com.tw\/?p=45194&amp;preview=true\"><strong><span style=\"color: #33cccc; font-size: 12pt;\">Minute\u2122 \u904e\u6c27\u5316\u7269\u9176\u6291\u88fd\u5291| Invent\u8ca8\u865fWA-005<\/span><\/strong><\/a><\/p>\n<p><span style=\"color: #999999; font-size: 12pt;\">Minute\u2122 Peroxidase Suppressor (30 ml)<\/span><\/p>\n<p><strong><a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/WA-005_v2.pdf?v=1613418055\" target=\"_blank\" rel=\"noopener\"><span>\u624b\u518a\u548c\u5354\u8b70<\/span><\/a><span>|\u00a0<\/span><a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-4_2d367d76-6026-4e18-b8da-e72f5e9d5d27.pdf?12736583005252625971\" target=\"_blank\" rel=\"noopener\"><span>\u6750\u6599\u5b89\u5168\u6578\u64da\u8868 (MSDS)<\/span><\/a><\/strong><\/p>\n<p><span>Minute\u2122 \u904e\u6c27\u5316\u7269\u9176\u6291\u88fd\u5291\u65e8\u5728\u6291\u5236\u514d\u75ab\u7d44\u7e54\u5316\u5b78 (IHC) \u7a0b\u5e8f\u4e2d\u5e38\u898b\u7684\u5167\u6e90\u6027\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u3002\u6291\u5236\u5167\u6e90\u6027\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u5c0d\u65bc\u907f\u514d\u5047\u967d\u6027\u548c\u964d\u4f4e IHC \u80cc\u666f\u81f3\u95dc\u91cd\u8981\u3002\u8a31\u591a\u5316\u5b78\u8a66\u5291\u5982H\u00a0<\/span><span>2\u00a0<\/span><span>O\u00a0<\/span><span>2<\/span><span>\u548c\u9209\u5df2\u88ab\u7528\u65bc\u6b64\u76ee\u7684\uff0c\u4f46\u6291\u5236\u901a\u5e38\u662f\u4e0d\u5b8c\u5168\u7684\u3002Minute\u2122 HRP Suppressor \u662f\u5e7e\u7a2e\u6709\u6548 HRP \u6291\u88fd\u5291\u7684\u6df7\u5408\u7269\uff0c\u5176\u7d50\u679c\u662f\u5b8c\u5168\u6291\u5236\u5167\u6e90\u6027\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u3002\u8a72\u6291\u5236\u5668\u53ef\u7528\u65bc\u5e38\u898f IHC \u4ee5\u53ca HRP \u4ecb\u5c0e\u7684\u8d85\u9748\u654f\u7a0b\u5e8f\uff0c\u4f8b\u5982\u916a\u9170\u80fa\u4fe1\u865f\u653e\u5927 (TSA)\u3002<\/span><\/p>\n<p><span>\u4e3b\u8981\u7279\u9ede\uff1a \u5728\u5ba4\u6eab\u4e0b\u975e\u5e38\u7a69\u5b9a\uff0c\u96a8\u6642\u53ef\u7528\uff0c\u4e26\u4e14\u4e0d\u53ef\u9006\u5730\u6291\u5236\u5167\u6e90\u6027\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u3002\u986f\u8457\u964d\u4f4e\u4f7f\u7528 HRP \u7684\u654f\u611f\u6aa2\u6e2c\u7684\u80cc\u666f\u3002<\/span><\/p>\n<ol>\n<li><span>\u5728\u8f09\u73bb\u7247\u4e0a\u88fd\u5099\u7d30\u80de\u6216\u7d44\u7e54\u6a23\u54c1\u5f8c\uff0c\u5982\u6709\u5fc5\u8981\uff0c\u57f7\u884c\u6297\u539f\u4fee\u5fa9\u6b65\u9a5f\u3002<\/span><\/li>\n<li><span>\u4f7f\u7528\u6b63\u5e38\u8840\u6e05\u6216\u60a8\u9078\u64c7\u7684\u5176\u4ed6\u5c01\u9589\u6eb6\u6db2\u5c01\u9589\u6a23\u54c1\u4e2d\u7684\u975e\u7279\u7570\u6027\u4f4d\u9ede\u3002<\/span><\/li>\n<li><span>\u6dfb\u52a0 1-2 \u6ef4 HRP \u6291\u88fd\u5291\u4ee5\u8986\u84cb\u8f09\u73bb\u7247\u4e0a\u7684\u6a23\u54c1\u3002\u5728\u6fd5\u5ea6\u5ba4\u4e2d\u5ba4\u6eab\u5b75\u80b2 10-15 \u5206\u9418\u3002\u7528 IHC \u6e05\u6d17\u7de9\u885d\u6db2\uff08\u4f8b\u5982 PBS-tween\uff09\u6e05\u6d17\u8f09\u73bb\u7247\u4e0a\u7684\u6a23\u54c1 3 \u6b21\uff0c\u4e26\u9032\u884c\u4e00\u6297\u5b75\u80b2\u3002<\/span><\/li>\n<li><span>\u91cd\u8981\u7684\u662f\uff0c\u5fc5\u9808\u5728\u6297\u539f\u4fee\u5fa9\u5f8c\u548c\u4e00\u6297\u5b75\u80b2\u524d\u6dfb\u52a0 HRP \u6291\u88fd\u5291\u3002\u5982\u679c\u5b75\u5316\u5f8c\u4e0d\u6d17\u6389\uff0c\u5b83\u53ef\u80fd\u6703\u5e72\u64fe\u6297\u539f\u6297\u9ad4\u7d50\u5408\u3002<\/span><\/li>\n<li><span>\u7e7c\u7e8c\u5176\u4ed6\u5be6\u9a57\u6b65\u9a5f\u3002<\/span><\/li>\n<\/ol>\n<p>Minute\u2122 Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positives and reducing the background of IHC. Many chemical agents such as H\u2082O\u2082\u00a0and sodium aside have been used for this purpose, but the inhibition is usually incomplete. Minute\u2122 HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibition of endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super-sensitive procedures such as tyramide signal amplification (TSA).<\/p>\n<p>Major Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduces the background of sensitive assays using HRP.<\/p>\n<ol>\n<li>After cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary.<\/li>\n<li>Block non-specific sites in the samples with normal serum or other blocking solution of your choice.<\/li>\n<li>Add 1-2 drops of HRP suppressor to cover the samples on the slide. Incubate at room temperature in a humidity chamber for 10-15 minutes. Wash the sample on the slide 3 times with an IHC washing buffer such as PBS-tween and perform primary antibody incubation.<\/li>\n<li>It is important that HRP suppressor must be added after antigen retrieval and before primary antibody incubation. It may interfere with antigen-antibody binding if it is not washed away after incubation.<\/li>\n<li>Continue with other experimental steps.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E9%81%8E%E6%B0%A7%E5%8C%96%E7%89%A9%E9%85%B6%E6%8A%91%E8%A3%BD%E5%8A%91-Invent%E8%B2%A8%E8%99%9FWA-005-2.webp\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Minute\u2122 \u904e\u6c27\u5316\u7269\u9176\u6291\u88fd\u5291Kit includes:<\/strong><\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>Item<\/strong><\/td>\n<td><strong>Quantity<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Peroxidase Suppressor<\/td>\n<td>30 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E9%81%8E%E6%B0%A7%E5%8C%96%E7%89%A9%E9%85%B6%E6%8A%91%E8%A3%BD%E5%8A%91-Invent%E8%B2%A8%E8%99%9FWA-005.webp\" width=\"351\" height=\"351\" \/><\/p>\n<p><strong><a href=\"https:\/\/inventbiotech.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%8E%9F%E5%BB%A0%E9%80%A3%E7%B5%9001-300x79.png\" alt=\"\u539f\u5ee0\u9023\u7d5001\" width=\"138\" height=\"36\" \/><\/a><\/strong><strong><a href=\"https:\/\/www.abmgood.com\/\"><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/a><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/strong><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u57fa\u65bc\u65cb\u8f49\u67f1(SPIN-COLUMN-BASED )\u7684\u86cb\u767d\u8cea\u63d0\u53d6\/\u7d30\u80de\u5206\u7d1a(CELL FRACTIONATION)<\/span><\/strong><\/span><\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-4356\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2015\/02\/Compined-F.gif\" alt=\"Compined-F\" width=\"446\" height=\"167\" \/><\/strong><\/p>\n<p>Invent Biotechnologies \u6210\u7acb\u65bc 2010 \u5e74\uff0c\u56e0\u9769\u65b0\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u800c\u4eab\u8b7d\u5168\u7403\u3002\u6211\u5011\u958b\u767c\u4e86\u57fa\u65bc\u65cb\u8f49\u67f1(spin column-based)\u7684\u5275\u65b0\u6280\u8853\uff0c\u7528\u65bc\u86cb\u767d\u8cea\u7814\u7a76( protein research)\uff0c\u53ef\u7c21\u5316\u548c\u589e\u5f37\u6a23\u54c1\u88fd\u5099\u3002\u9019\u6703\u5e36\u4f86\u66f4\u9806\u66a2\u7684\u5de5\u4f5c\u6d41\u7a0b\u548c\u59cb\u7d42\u53ef\u9760\u7684\u7d50\u679c\uff0c\u5f9e\u800c\u5e36\u4f86\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002\u9078\u64c7 Minute\u2122 \u8a66\u5291\u76d2\u9032\u884c\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2(cell fractionation)\u7684\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u76f8\u4fe1\uff0c\u4ed6\u5011\u7684\u6a23\u54c1\u6e96\u78ba\u5730\u4ee3\u8868\u4e86\u6240\u7814\u7a76\u7684\u7d30\u80de\u6216\u7d44\u7e54\u7684\u81ea\u7136\u7279\u5fb5\u3002\u9019\u4e9b\u5957\u4ef6\u53ea\u9700\u8981\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\uff0c\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u5be6\u9a57\u8b8a\u91cf\u4e26\u78ba\u4fdd\u9ad8\u8cea\u91cf\u7684\u6750\u6599\u4ee5\u7372\u5f97\u66f4\u53ef\u9760\u7684\u6578\u64da\u3002Minute\u2122 \u65b9\u6cd5\u7684\u6709\u6548\u6027\u5f97\u5230\u4e86\u8d8a\u4f86\u8d8a\u591a journals\u7684\u652f\u6301 (Nature, Science, and Cell.)\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #ff0000;\"><b>The Minute\u2122 \u7684\u512a\u9ede\uff1a<\/b><\/span><br \/>\n\u2022 \u8d85\u5feb\uff08\u5feb\u81f3 1 \u5206\u9418\uff09<br \/>\n\u2022 \u6a23\u672c\u91cf\u5c0f<br \/>\n\u2022 \u6700\u5c0f\u5316\u4ea4\u53c9\u6c61\u67d3<br \/>\n\u2022 \u5167\u6e90\u6027\u57fa\u7dda\u4e0d\u8b8a<br \/>\n\u2022 \u6240\u6709\u6d3b\u9ad4\u548c\u7d44\u7e54\u985e\u578b<\/p>\n<p><span style=\"color: #ff0000;\"><strong><span style=\"font-size: 12pt;\">The Minute\u2122 \u61c9\u7528\uff1a<\/span><\/strong><\/span><br \/>\n\u2022 \u86cb\u767d\u8cea\u904b\u8f38\/\u6613\u4f4d<br \/>\n\u2022 \u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528<br \/>\n\u2022 \u86cb\u767d\u8cea\u4fee\u98fe<br \/>\n\u2022 \u4fe1\u865f\u8f49\u5c0e<br \/>\n\u2022 sc\/snRNA-seq<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">Invent Minute\u2122 \u5fb9\u5e95\u6539\u8b8a\u86cb\u767d\u8cea\u6a23\u54c1\u88fd\u5099\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2<\/span><\/strong><\/p>\n<div class=\"rte\">\n<p>\u81ea 2010 \u5e74\u6210\u7acb\u4ee5\u4f86\uff0c\u6211\u5011\u7684\u9996\u8981\u76ee\u6a19\u662f\u5229\u7528\u6211\u5011\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u5275\u65b0\u6280\u8853\u5fb9\u5e95\u6539\u8b8a\u5168\u7403\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u5e02\u5834\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u8b93 Minute\u2122 \u8a66\u5291\u76d2\u6210\u70ba\u6a19\u6e96\u5be6\u9a57\u5ba4\u7a0b\u5e8f\uff0c\u53d6\u4ee3\u6240\u6709\u57fa\u65bc\u6eb6\u6db2\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u3002\u901a\u904e\u5229\u7528\u9019\u4e9b\u5957\u4ef6\uff0c\u7814\u7a76\u4eba\u54e1\u53ea\u9700\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\u5373\u53ef\u7372\u5f97\u4e00\u81f4\u7684\u7d50\u679c\u4e26\u7372\u5f97\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/5-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"491\" height=\"239\" \/><\/p>\n<p><span style=\"font-size: 12pt;\"><strong><span style=\"color: #800000;\">\u6539\u8b8a\u86cb\u767d\u8cea\u63d0\u53d6(Transforming protein extraction)<\/span><\/strong><\/span><\/p>\n<p>Minute\u2122 \u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1( simple spin column )\uff0c\u53ef\u6709\u6548\u6d88\u9664\u7d30\u80de\u6216\u7d44\u7e54\u88c2\u89e3\u904e\u7a0b(lysis)\u4e2d\u7684\u975e\u7cfb\u7d71\u6027\u86cb\u767d\u8cea\u640d\u5931(non-systematic protein loss)\u3002\u9019\u4ee3\u8868\u8457\u76f8\u5c0d\u65bc\u50b3\u7d71\u7684\u57fa\u65bc\u89e3\u6c7a\u65b9\u6848\u7684\u65b9\u6cd5\uff0c\u4f8b\u5982 RIPA \u7de9\u885d\u6db2(RIPA buffer)\u548c\u8d85\u8072\u8655\u7406(sonication)\u7684\u986f\u8457\u9032\u6b65\uff0c\u9019\u4e9b\u65b9\u6cd5\u4ee5\u5176\u53ef\u8b8a\u6027\u548c\u7121\u6cd5\u63d0\u4f9b\u5168\u9762\u7684\u86cb\u767d\u8cea\u8b5c\u800c\u805e\u540d\u3002\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\u7684 Minute\u2122 \u8a66\u5291\u76d2\u8207\u4f86\u81ea\u5404\u7a2e\u751f\u7269\u9ad4\u548c\u7d44\u7e54\u985e\u578b\u7684\u5c0f\u6a23\u672c\u91cf\u517c\u5bb9\uff0c\u70ba\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u4e86\u672a\u6539\u8b8a\u7684\u5167\u6e90\u86cb\u767d\u57fa\u7dda( endogenous proteins)\uff0c\u5f9e\u800c\u5be6\u73fe\u66f4\u7cbe\u78ba\u7684\u5b9a\u91cf\u548c\u5206\u6790\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-Biotechs-Minute-Kit-shows-a-more-complete-cell-lysis-compared-to-RIPA-buffer-in-which-a-pellet-remains-leading-to-an-alteration-of-the-protein-profile.jpg\" width=\"374\" height=\"481\" \/><\/p>\n<p>Invent Biotech&#8217;s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/3-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"488\" height=\"243\" \/><\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">\u6539\u9032\u7d30\u80de\u5206\u7d1a\u5206\u96e2(Revamping cell fractionation)<\/span><\/strong><\/p>\n<p>\u7531\u65bc\u7f3a\u4e4f\u7c21\u55ae\u7684\u5be6\u9a57\u65b9\u6848\uff0c\u50b3\u7d71\u7684\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u65b9\u6cd5\u9577\u671f\u4ee5\u4f86\u4e00\u76f4\u53d7\u5230\u7528\u6236\u504f\u5dee\u548c\u7d50\u679c\u4e0d\u4e00\u81f4\u7684\u5f71\u97ff\u3002 Minute\u2122 \u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2\u5fb9\u5e95\u6539\u8b8a\u4e86\u9019\u4e00\u904e\u7a0b\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u5728 90 \u5206\u9418\u5167\u7372\u5f97\u9ad8\u7d14\u5ea6\u7684\u7d30\u80de\u5668\u7fa4\uff0c\u4e26\u4e14\u4f7f\u7528\u6700\u5c11\u7684\u6d17\u6ecc\u5291\u3002\u901a\u904e\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1\uff0c\u9019\u4e9b\u8a66\u5291\u76d2\u7121\u9700\u5747\u8cea\u5316(homogenization)\u3001\u5bc6\u5ea6\u68af\u5ea6\u5206\u96e2(density gradient separation)\u548c\u8d85\u901f\u96e2\u5fc3\u7b49\u50b3\u7d71\u6280\u8853( ultra-centrifugation)\uff0c\u5f9e\u800c\u6e1b\u5c11\u4e86\u5be6\u9a57\u8b8a\u91cf\u7684\u6578\u91cf\u3002\u9019\u53ef\u4ee5\u9632\u6b62\u7d30\u80de\u5668\u640d\u50b7( organelle damage)\u4e26\u7522\u751f\u66f4\u4e00\u81f4\u548c\u751f\u7406\u76f8\u95dc\u7684\u7d50\u679c\u3002\u6191\u85c9\u591a\u7a2e Minute\u2122 \u8a66\u5291\u76d2\u53ef\u6eff\u8db3\u4e0d\u540c\u7684\u7d30\u80de\u5206\u7d1a(cell fractionation)\u5206\u96e2\u8981\u6c42\uff0c\u6211\u5011\u6b63\u5728\u63a8\u52d5\u86cb\u767d\u8cea\u7d44\u7814\u7a76\u7684\u8f49\u578b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/2-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"480\" height=\"238\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u652f\u6301\u6240\u6709\u7814\u7a76\u5b78\u79d1(Supporting all research disciplines)<\/strong><\/span><\/p>\n<p>Minute\u2122 \u8a66\u5291\u76d2\u65e8\u5728\u7121\u7e2b\u96c6\u6210\u5230\u73fe\u6709\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u63d0\u4f9b\u5feb\u901f\u4e14\u4e00\u81f4\u7684\u6027\u80fd\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u7d50\u679c\u3002\u7136\u800c\uff0c\u4e0d\u8981\u53ea\u76f8\u4fe1\u6211\u5011\u7684\u8a71\u2014\u2014\u6211\u5011\u7684\u7522\u54c1\u5df2\u5728\u79d1\u5b78\u6587\u737b\u4e2d\u88ab\u5ee3\u6cdb\u5f15\u7528\uff0c\u9019\u8868\u660e\u7814\u7a76\u754c\u5c0d\u7c21\u5316\u4e14\u53ef\u9760\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u7684\u9700\u6c42\u4e0d\u65b7\u589e\u9577\u3002\u7acb\u5373\u5207\u63db\u5230 Minute\u2122\uff0c\u9ad4\u9a57\u6211\u5011\u6280\u8853\u7684\u512a\u52e2\uff0c\u7121\u8ad6\u60a8\u7684\u7814\u7a76\u9818\u57df\u5982\u4f55\u3002<\/p>\n<table style=\"height: 2673px;\" width=\"995\">\n<tbody>\n<tr>\n<td width=\"64\">SD-001\/SN-002<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-003<\/td>\n<td>Minute <sup>TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-006<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005-p<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MP-007<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-038<\/td>\n<td>Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SD-008\/SN-009<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-010<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CP-011<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SB-004<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-012<\/td>\n<td>Minute <sup>TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NE-013<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HP-014<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YT-015<\/td>\n<td width=\"667\">Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YD-016<\/td>\n<td width=\"667\">Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YM-017<\/td>\n<td>Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-018<\/td>\n<td width=\"667\">Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-019<\/td>\n<td width=\"667\">Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HD-021<\/td>\n<td width=\"667\">MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AT-022<\/td>\n<td width=\"667\">MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AF-023<\/td>\n<td>MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NI-024<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">BN-020<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-047<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AN-029<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">FE-025<\/td>\n<td>Minute<sup>TM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MS-026<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Mass Spectrometry (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">EI-027<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ED-028<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Endosome Isolation and Cell Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SE-030<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CS-031<\/td>\n<td>Minute<sup>TM<\/sup> Cell Suspension Isolation from Fresh\/Frozen Tissues (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NT-032<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NM-033<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LY-034<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-035<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> High Fidelity ER Isolation Kit for Liver Tissue (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-036<\/td>\n<td>Minute<sup>TM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">GO-037<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-039<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-042<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PT-040<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-041<\/td>\n<td>Minute<sup>TM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PF-045<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SV-043<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ML-044<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">RD-046<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Rubisco Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PR-048<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant ER Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PG-049<\/td>\n<td>Minute<sup>TM<\/sup> Plant Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PE-050<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Endosome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PL-051<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Lipid Raft Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SY-052<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Synaptosome Isolation Kit (50 preps) NEW!<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-01-SK<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-02-BT<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-03-BV<\/td>\n<td>Minute<sup>TM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-04-TD<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-05-IM<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-06-MS<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-07-IS<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-002<\/td>\n<td width=\"667\">Minute\u2122 Ab Enhancer (30 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-003<\/td>\n<td width=\"667\">Minute\u2122 WB Stripping Solution (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-004<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Ponceau S Stain (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Peroxidase Suppressor (30 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-006<\/td>\n<td>Minute <sup>TM<\/sup> High Efficiency Protein Precipitation kit<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-007<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> RBC Lysis Buffer (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-008<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> WB Blocking Solution (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-009<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Denaturing Protein Solubilization Reagent (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-010<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Non-Denatured Protein Solubilization Reagent (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-011<\/td>\n<td>Minute<sup>TM<\/sup> Protein Solubilization Reagent for MS (2 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-012<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> SDS-Remover (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-013<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Albumin Depletion Reagent for Plasma and Serum (20 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-014<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Anti-Clumping Nuclei Storage Buffer NEW!<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-Rapid Protein Extraction Kits Minute\u2122\u86cb\u767d\u8403\u53d6\u5957 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5889],"tags":[6268],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45194"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45194"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45194\/revisions"}],"predecessor-version":[{"id":45195,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45194\/revisions\/45195"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45194"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45194"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45194"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}