{"id":45011,"date":"2023-08-11T22:25:21","date_gmt":"2023-08-11T14:25:21","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45011"},"modified":"2023-08-30T11:31:21","modified_gmt":"2023-08-30T03:31:21","slug":"%ce%b2-%e8%91%a1%e8%90%84%e7%b3%96%e8%8b%b7%e9%85%b6%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-beta-glucosidase-assay-kit-abnova%e8%b2%a8%e8%99%9fka1611","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=45011","title":{"rendered":"\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0| beta-Glucosidase Assay Kit ABNOVA\u8ca8\u865fKA1611"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=45011&amp;preview=true\"><span style=\"font-size: 12pt;\">\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2 \u00a0| beta-Glucosidase Assay Kit ABNOVA\u8ca8\u865fKA1611<\/span><\/a><\/span><\/p>\n<p><span style=\"color: #999999;\">\u7dda\u6027\u6aa2\u6e2c\u7bc4\u570d2 U\/L- 250U\/L (2 mU\/ml &#8211; 250 mU\/ml)<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/abnova.png\" width=\"628\" height=\"82\" \/><\/p>\n<p>\u03b2-\u8461\u8404\u7cd6\u82f7\u9176(\u03b2-GLUCOSIDASE)\u662f\u4e00\u7a2e\u8461\u8404\u7cd6\u82f7\u9176( glucosidase enzyme)\uff0c\u4f5c\u7528\u65bc\u9023\u63a5\u5169\u500b\u8461\u8404\u7cd6\u6216\u03b21-&gt;4 \u9375(glucose-substituted molecule)\u8461\u8404\u7cd6\u53d6\u4ee3\u7684\u5206\u5b50\uff08\u5373\u4e8c\u7cd6\u7e96\u7dad\u4e8c\u7cd6\uff09\u3002\u751f\u7269\u9ad4\u9700\u8981\u03b2-\u8461\u8404\u7cd6\u82f7\u9176) (\u03b2-Glucosidases)\uff0c\u67d0\u4e9b\u771f\u83cc(fungi)\u3001\u7d30\u83cc(bacteria)\u3001\u767d\u87fb( termites\uff09\u7528\u65bc\u6d88\u8017\u7e96\u7dad\u7d20(cellulose)\u3002\u6eb6\u83cc\u9176( Lysozyme)\u4e5f\u662f\u4e00\u7a2e \u03b2-\u8461\u8404\u7cd6\u82f7\u9176(\u03b2-glucosidase )\u4e26\u4e14\u5b58\u5728\u9632\u6b62\u773c\u775b\u7d30\u83cc\u611f\u67d3\u3002\u5728\u4eba\u985e\u4e2d\uff0c\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u7570\u69cb\u9ad4\u7684\u6d3b\u6027\u8f03\u4f4e\uff0c\u6eb6\u9176\u9ad4\u8461\u8404\u7cd6\u8166\u82f7\u9176(lysosomal gluco-cerebrosidase)\u8207\u6208\u8b1d\u75c5( Gaucher&#8217;s disease)\u548c\u5e15\u91d1\u68ee\u75c5( Parkinson&#8217;s disease)\u6709\u95dc\u3002\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2(Beta-Glucosidase Assay Kit)\u65e8\u5728\u76f4\u63a5\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\u4e2d\u7684 \u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027( \u03b2-glucosidase activity)\u3002\u7121\u9700\u9810\u8655\u7406\u3002\u6539\u9032\u7684\u65b9\u6cd5\u5229\u7528\u6c34\u89e3\u7684\u5c0d\u785d\u57fa\u82ef\u57fa-\u03b2-D-\u5421\u5583\u8461\u8404\u7cd6\u82f7( p-nitrophenyl-\u03b2-D-glucopyranoside)\u901a\u904e \u03b2-\u8461\u8404\u7cd6\u82f7\u9176(\u03b2-glucosidase)\u8f49\u5316\u70ba\u9ec3\u8272\u7522\u7269\uff08\u6700\u5927\u5438\u5149\u5ea6\u70ba 405 nm\uff09\u7684\u6bd4\u7387\u53cd\u61c9\u8207\u9176\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/%CE%B2-%E8%91%A1%E8%90%84%E7%B3%96%E8%8B%B7%E9%85%B6%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-beta-Glucosidase-Assay-Kit-ABNOVA%E8%B2%A8%E8%99%9FKA1611.jpg\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/03\/%E7%94%A2%E5%93%81%E7%89%B9%E9%BB%9E_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7522\u54c1\u7279\u9ede_\u5de5\u4f5c\u5340\u57df 1\" width=\"145\" height=\"59\" \/><\/p>\n<p><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713 \u76f4\u63a5\u6e2c\u5b9a\uff1a\u751f\u7269\u6a23\u54c1\u4e2d\u7684 \u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u3002<\/span><\/p>\n<p><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713 \u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u751f\u7522\u7684\u8868\u5fb5\u548c\u8cea\u91cf\u63a7\u5236\u3002 <\/span><\/p>\n<p><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713 \u85e5\u7269\u767c\u73fe\uff1a\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u8abf\u7bc0\u5291\u7684\u9ad8\u901a\u91cf\u7be9\u9078<br \/>\n\u2713 \u9ad8\u9748\u654f\u5ea6\u548c\u5bec\u7dda\u6027\u7bc4\u570d\uff1a\u4f7f\u752820 \u00b5L \u6a23\u54c1\u3002\u6aa2\u6e2c\u9650\u70ba2 U\/L\uff0c\u7dda\u6027\u9ad8\u9054250 U\/L\u3002 <\/span><\/p>\n<p><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713 \u5747\u8cea\u4e14\u7c21\u55ae\u7684\u7a0b\u5e8f\uff1a\u7c21\u55ae\u7684\u201c\u6df7\u5408\u548c\u6e2c\u91cf\u201d\u7a0b\u5e8f\u53ef\u5be6\u73fe\u53ef\u9760\u7684\u5b9a\u91cf 20\u5206\u9418\u5167\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6d3b\u6027\u3002<\/span><\/p>\n<p><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713 \u7a69\u5065\u4e14\u9069\u5408 HTS\uff1a\u6240\u6709\u8a66\u5291\u5747\u8207\u9ad8\u901a\u91cf\u6db2\u9ad4\u8655\u7406\u517c\u5bb9\u5100\u5668\u3002<\/span><\/p>\n<p><a href=\" http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/1-\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2-beta-Glucosidase-Assay-Kit-ABNOVA\u8ca8\u865fKA1611.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/2-%CE%B2-%E8%91%A1%E8%90%84%E7%B3%96%E8%8B%B7%E9%85%B6%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-beta-Glucosidase-Assay-Kit-ABNOVA%E8%B2%A8%E8%99%9FKA1611.png\" \/><\/p>\n<p>\u9176\u6a23\u54c1(Enzyme samples )\u53ef\u4ee5\u5728 50 mM \u78f7\u9178\u9e7d (pH 7.0) \u7de9\u885d\u6db2\u4e2d\u6216\u4efb\u4f55\u5176\u4ed6\u5408\u9069\u7684\u9176\u7de9\u885d\u6db2\u4e2d\u3002\u5df2\u77e5\u4ee5\u4e0b\u5316\u5b78\u7269\u8cea\u6703\u5f71\u97ff\u9176\u6d3b\u6027\uff0c\u61c9\u907f\u514d\u4f7f\u7528\u3002\u542b SH \u7684\u8a66\u5291\uff08\u4f8b\u5982\u4e8c\u786b\u8607\u7cd6\u9187\u30012-\u5df0\u57fa\u4e59\u9187\u3001\u7a40\u80f1\u7518\u80bd\uff09\u3001Ca2+\u3001Cu2+\u3001Fe3+\/Fe2+\u3001Hg2+\u3001Mg2+\u3001Ni2+\u3001Zn2+\u3001SDS\u3001EDTA \u548c Tris\u3002<\/p>\n<div id=\"0\">\n<p><span style=\"font-size: 12pt; color: #993366;\"><b>Specification<\/b><\/span><\/p>\n<\/div>\n<div id=\"10000\">\n<div id=\"0\">\n<ul class=\"first_title\">\n<li class=\"blue12\"><b>Specification<\/b><\/li>\n<\/ul>\n<\/div>\n<div id=\"10000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Product Description:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">beta-Glucosidase Assay Kit is a colorimetric kinetic determination of beta-Glucosidase activity.<\/li>\n<\/ul>\n<\/div>\n<div id=\"10200\">\n<ul>\n<li class=\"black12 sub_title\"><b>Sample Volume:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">20 uL<\/li>\n<\/ul>\n<\/div>\n<div id=\"10400\">\n<ul>\n<li class=\"black12 sub_title\"><b>Detection Method:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Colorimetric<\/li>\n<\/ul>\n<\/div>\n<div id=\"30000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Limit of Detection:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">2 U\/L<\/li>\n<\/ul>\n<\/div>\n<div id=\"260100\">\n<ul>\n<li class=\"black12 sub_title\"><b>Regulation Status:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">For research use only (RUO)<\/li>\n<\/ul>\n<\/div>\n<div id=\"400000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Storage Instruction:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Store the kit at -20\u00b0C.<\/li>\n<\/ul>\n<\/div>\n<div id=\"410000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Note:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Result Data<\/li>\n<li class=\"black11_a_underline\"><img decoding=\"async\" src=\"https:\/\/www.abnova.com\/note_image\/note-KA1611-1.jpg\" \/><\/li>\n<li class=\"black11_a_underline\">Kinetics of beta-glucosidase reaction in 96-well plate assay.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div>\n<p><span style=\"font-size: 12pt; color: #800000;\"><b>Publication Reference<\/b><\/span><\/p>\n<p>1.<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/34035300\" target=\"_new\" rel=\"noopener\">Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson&#8217;s disease.<\/a><br \/>\nHideaki Matsui, Junko Ito, Noriko Matsui, Tamayo Uechi, Osamu Onodera, Akiyoshi Kakita.Nat Commun. 2021 May 25;12(1):3101. doi: 10.1038\/s41467-021-23452-x.<\/p>\n<p>2.<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29503608\" target=\"_new\" rel=\"noopener\">Silencing of Glucocerebrosidase Gene in Drosophila Enhances the Aggregation of Parkinson&#8217;s Disease Associated \u03b1-Synuclein Mutant A53T and Affects Locomotor Activity.<\/a><br \/>\nAbul Khair SB, Dhanushkodi NR, Ardah MT, Chen W, Yang Y, Haque ME.Front Neurosci. 2018 Feb 16;12:81. doi: 10.3389\/fnins.2018.00081. eCollection 2018.<\/p>\n<p>3.<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28413443\" target=\"_new\" rel=\"noopener\">Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production.<\/a><br \/>\nIbrahim E, Jones KD, Taylor KE, Hosseney EN, Mills PL, Escudero JM.Biotechnol Biofuels. 2017 Feb 27;10:52.<\/p>\n<p>4.<a href=\"https:\/\/peerj.com\/articles\/2168\/\" target=\"_new\" rel=\"noopener\">The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells<\/a><br \/>\nAttia RT, Tolba MF, Trivedi R, Tadros MG, Arafa HMM, Abdel-Naim AB.Peer J. 2016 Jun 29. [Epub ahead of print]<\/p>\n<\/div>\n<div id=\"420000\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u03b2-\u8461\u8404\u7cd6\u82f7\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2 \u00a0| beta-Glucosidase Assay Kit ABNOVA\u8ca8\u865fKA16 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6042],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45011"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45011"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45011\/revisions"}],"predecessor-version":[{"id":45444,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45011\/revisions\/45444"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45011"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45011"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45011"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}