{"id":44895,"date":"2023-08-07T16:41:29","date_gmt":"2023-08-07T08:41:29","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44895"},"modified":"2023-10-22T12:38:38","modified_gmt":"2023-10-22T04:38:38","slug":"minute-%e7%84%a1%e5%8e%bb%e5%9e%a2%e5%8a%91%e5%96%ae%e6%a0%b8%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92detergent-free-single-nuclei-isolation-kit-invent%e8%b2%a8%e8%99%9fni-024","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44895","title":{"rendered":"Minute\u2122 \u7121\u53bb\u57a2\u5291\u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2|Detergent-Free Single Nuclei Isolation Kit Invent\u8ca8\u865fNI-024"},"content":{"rendered":"<p><strong><span style=\"font-size: 14pt; color: #800000;\">\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-<span style=\"color: #ff9900;\">Rapid Protein Extraction Kits<\/span><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-minute_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1.jpg\" \/><\/p>\n<p><strong>Minute\u2122<\/strong><strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300eColumn Filter\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong><strong>\u00a0<\/strong><strong>\u4e00 \u5206\u9418<\/strong><strong>\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<em>vortex\u3001transfer\u3001spin \u3002<\/em><\/strong>\u907f\u514d RIPA \u4e0d\u6eb6\u6027\u86cb\u767d\u8cea\u7684\u554f\u984c\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u65b9\u6cd5\u7372\u5f97\u66f4\u5b8c\u6574\u7684\u86cb\u767d\u8cea\u8b5c\u3002Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n<p><iframe loading=\"lazy\" title=\"Minute Total Protein Extraction Kit - Invent Biotechnologies\" src=\"\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" width=\"562\" height=\"316\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/p>\n<p><a href=\"https:\/\/biopioneer.com.tw\/?p=44895&amp;preview=true\"><strong><span style=\"color: #33cccc; font-size: 12pt;\">Minute\u2122 \u7121\u53bb\u57a2\u5291\u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2 | Invent\u8ca8\u865fNI-024<\/span><\/strong><\/a><\/p>\n<p><span style=\"color: #999999; font-size: 12pt;\">Minute\u2122 Detergent-Free Single Nuclei Isolation Kit (20 Preps)<\/span><\/p>\n<p><strong><a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/24-NI-024_v8_b4da52af-ee41-4e2b-8b6d-29b1cd3b02be.pdf?v=1645732137\" target=\"_blank\" rel=\"noopener\">\u624b\u518a\u548c\u5354\u8b70<\/a>\u00a0|\u00a0<a href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1-2_198858e0-6493-427b-8682-369e42a2a92c.pdf?13345605419166362649\" target=\"_blank\" rel=\"noopener\">\u6750\u6599\u5b89\u5168\u6578\u64da\u8868 (MSDS)\u00a0|\u00a0<\/a><\/strong><a id=\"ui-id-2\" class=\"ui-tabs-anchor\" tabindex=\"-1\" role=\"presentation\" href=\"https:\/\/inventbiotech.com\/collections\/single-nuclei-isolation\/products\/minute-detergent-free-nuclei-isolation-kit#tabs-3\">\u53c3\u8003\u6587\u737b\uff087\uff09<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong><em>*Invent Biotechnologies, Inc. \u662f\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u6280\u8853\uff08\u5305\u62ec\u55ae\u6838\u5206\u96e2\uff09\u7684\u767c\u660e\u8005\u00a0\u3002\u6211\u5011\u5c1a\u672a\u6388\u6b0a\u7b2c\u4e09\u65b9\u4f7f\u7528\u8a72\u6280\u8853\u751f\u7522\u985e\u4f3c\u7522\u54c1\u00a0\u3002\u00a0<\/em><\/strong><\/p>\n<p><strong>\u89c0\u770b JoVE \u4e0a\u7684\u6700\u65b0\u8996\u983b<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong><em>*Invent Biotechnologies, Inc. is the\u00a0inventor of the\u00a0spin column-based cell fractionation technology (including single nuclei isolation). We have not authorized third parties to produce similar products using the\u00a0technology.\u00a0<\/em><\/strong><\/p>\n<p><strong>Watch the newest Video on JoVE<\/strong><\/p>\n<p><iframe loading=\"lazy\" id=\"embed-iframe\" src=\"https:\/\/www.jove.com\/embed\/player?id=61471&amp;s=1&amp;fpv=1\" width=\"460\" height=\"260\" frameborder=\"0\" marginwidth=\"0\" scrolling=\"no\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/p>\n<p><span>\u55ae\u7d30\u80de\u57fa\u56e0\u7d44\u5b78\u9700\u8981\u4f86\u81ea\u55ae\u500b\u7d30\u80de\u7684\u9ad8\u8cea\u91cf\u548c\u5b8c\u6574\u7684\u907a\u50b3\u7269\u8cea\u3002\u7136\u800c\uff0c\u5b8c\u6574\u7684\u55ae\u7d30\u80de\u5f88\u96e3\u5f9e\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u4f86\uff0c\u5c24\u5176\u662f\u5728\u51b7\u51cd\u6642\u3002\u6b64\u5916\uff0c<\/span><span>\u89e3\u96e2\u8a98\u5c0e\u7684\uff08\u6a5f\u68b0\u6216\u9176\u4fc3\uff09\u7fa4\u9ad4\u548c\u8868\u9054\u504f\u5dee\u4e5f\u662f\u6709\u554f\u984c\u7684\u3002\u5206\u96e2\u5b8c\u6574\u7684\u7d30\u80de\u6838\u4f5c\u70ba\u5927\u591a\u6578\u55ae\u7d30\u80de\u57fa\u56e0\u7d44\u5b78\u61c9\u7528\u7684\u8d77\u59cb\u6750\u6599\u66f4\u70ba\u53ef\u884c\u3002\u00a0<\/span><\/p>\n<p><span>\u7121\u53bb\u6c61\u5291\u7d30\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2\u65e8\u5728\u5f9e\u52d5\u7269\u57f9\u990a\u7d30\u80de\u6216\u7d44\u7e54\uff08\u65b0\u9bae\u6216\u51b7\u51cd\uff09\u4e2d\u5feb\u901f\u5206\u96e2\u5b8c\u6574\u7684\u7d30\u80de\u6838\u3002\u4f7f\u7528\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u5c08\u6709\u6280\u8853\uff0c\u53ef\u4ee5\u5728\u4e0d\u5230 20 \u5206\u9418\u7684\u6642\u9593\u5167\u5f9e\u6a23\u54c1\u4e2d\u5206\u96e2\u51fa\u5b8c\u6574\u7684\u7d30\u80de\u6838\uff0c\u800c\u7121\u9700\u4f7f\u7528\u7d44\u7e54\u52fb\u6f3f\u5668\u548c\u4efb\u4f55\u6d17\u6ecc\u5291\u3002\u50b3\u7d71\u7684\u7d30\u80de\u6838\u5206\u96e2\u65b9\u6cd5\u6d89\u53ca\u4f7f\u7528\u975e\u96e2\u5b50\u53bb\u6c61\u5291\uff0c\u9019\u5bb9\u6613\u5c0e\u81f4\u4e0d\u5fc5\u8981\u7684\u7d30\u80de\u6838\u805a\u96c6\u3002\u76ee\u524d\u9084\u4e0d\u6e05\u695a\u70ba\u4ec0\u9ebc\u6709\u4e9b\u539f\u5b50\u6838\u6703\u805a\u96c6\uff0c\u800c\u53e6\u4e00\u4e9b\u5247\u4e0d\u6703\u3002\u9084\u4e0d\u6e05\u695a\u975e\u805a\u96c6\u6838\u662f\u5426\u662f\u6574\u500b\u6838\u7fa4\u7684\u516c\u6b63\u4ee3\u8868\u3002\u6e05\u6f54\u5291\u9084\u6709\u53ef\u80fd\u640d\u58de\u6838\u819c\uff0c\u5c0e\u81f4\u6838\u57fa\u8cea\u6750\u6599\u6d29\u6f0f\u3002\u5c0d\u65bc\u67d0\u4e9b\u7d30\u80de\/\u7d44\u7e54\u985e\u578b\uff0c\u53bb\u6c61\u5291\u4e5f\u53ef\u4ee5\u525d\u96e2\u6838\u5305\u819c\u86cb\u767d\u3002\u5206\u9418<\/span><span>\u8207\u50b3\u7d71\u65b9\u6cd5\u76f8\u6bd4\uff0c<sup>TM\u7121\u6d17\u6ecc\u5291\u7d30\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5168\u65b0\u7684\u7d30\u80de\u6838\u5206\u96e2\u65b9\u6cd5\u3002<\/sup><\/span><\/p>\n<p><strong><span>\u5de5\u4f5c\u539f\u7406\uff1a<\/span><\/strong><span>\u7d30\u80de\/\u7d44\u7e54\u9996\u5148\u88ab\u7de9\u885d\u6db2 A \u654f\u5316\uff0c\u7136\u5f8c\u5728\u65bd\u52a0\u9ad8\u901f\u96e2\u5fc3\u529b\u6642\u4ee5\u4e4b\u5b57\u5f62\u65b9\u5f0f\u901a\u904e\u5c08\u6709\u7684\u904e\u6ffe\u5668\u3002\u7d30\u80de\u5728\u901a\u904e\u904e\u6ffe\u5668\u6642\u7834\u88c2\uff0c\u5728\u6d41\u904e\u7269\u4e2d\u7559\u4e0b\u5b8c\u6574\u7684\u5929\u7136\u7d30\u80de\u6838\u3002\u4f7f\u7528\u5c08\u6709\u7de9\u885d\u6db2 B \u901a\u904e\u4f4e\u901f\u96e2\u5fc3\u5c07\u7d30\u80de\u6838\u8207\u5176\u4ed6\u5c0f\u7d30\u80de\u788e\u7247\u5206\u96e2\u3002\u5206\u96e2\u51fa\u7684\u5929\u7136\u4e14\u5b8c\u6574\u7684\u7d30\u80de\u6838\u53ef\u7528\u65bc\u5404\u7a2e\u4e0b\u6e38\u61c9\u7528\uff0c\u5305\u62ec\u4f46\u4e0d\u9650\u65bc\uff1aFACS \u5206\u6790\u3001\u55ae\u6838\u5206\u6790\uff08\u4f8b\u5982RNA-seq \u548cATAC-seq\uff09\u3001\u514d\u75ab\u7192\u5149\u67d3\u8272\u3001\u7d30\u80de\u9031\u671f\u5206\u6790\u548c\/\u6216\u7d30\u80de\u51cb\u4ea1\u7814\u7a76\u3002<\/span><\/p>\n<p><em><strong><span>\u5c0d\u65bc\u5206\u96e2\u7684\u55ae\u6838\u7684\u5132\u5b58\u548c\u904b\u8f38\uff0c\u5efa\u8b70\u4f7f\u7528\u4ee5\u4e0b\u7de9\u885d\u6db2\uff1a<\/span><\/strong><\/em><\/p>\n<p><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-anti-clumping-nuclei-storage-buffer-10-ml?_pos=1&amp;_sid=8b67760c6&amp;_ss=r\" target=\"_blank\" rel=\"noopener\"><em><span>WA-014\uff1aMinute\u2122 \u6297\u7d50\u584a\u7d30\u80de\u6838\u5132\u5b58\u7de9\u885d\u6db2\u00a0<\/span><\/em><\/a><\/p>\n<p><em><strong><span>\u6ce8\uff1a<\/span><\/strong><span>\u5982\u679c\u4e0d\u64d4\u5fc3\u6d17\u6ecc\u5291\u7684\u5b58\u5728\uff0c\u6211\u5011\u9084\u63d0\u4f9b\u4ee5\u4e0b\u55ae\u6838\u5206\u96e2\u5957\u4ef6\uff0c\u5176\u80cc\u666f\u66f4\u52a0\u5e72\u6de8\uff1a<\/span><\/em><\/p>\n<p><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=1&amp;_sid=2bc6f4939&amp;_ss=r\" target=\"_blank\" rel=\"noopener\"><span>SN-047\uff1a\u7528\u65bc\u7d44\u7e54\/\u7d30\u80de\u7684 Minute\u2122 \u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2<\/span><\/a><\/em><\/p>\n<p><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-neuronal-tissues-cells-20-preps?_pos=1&amp;_sid=1f449fc5a&amp;_ss=r\" target=\"_blank\" rel=\"noopener\"><span>BN-020\uff1a\u7528\u65bc\u795e\u7d93\u7d44\u7e54\/\u7d30\u80de\u7684 Minute\u2122 \u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2<\/span><\/a><\/em><\/p>\n<p><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-nuclei-and-cytosol-isolation-kit-for-adipose-tissues?_pos=1&amp;_sid=f91e6c921&amp;_ss=r\" target=\"_blank\" rel=\"noopener\"><span>AN-029\uff1a\u7528\u65bc\u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u7684 Minute\u2122 \u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/span><\/a><\/em><\/p>\n<p>Single-cell genomics requires high-quality and complete genetic materials from individual cells. However, intact single cells are hard to isolate from tissues, especially when frozen. In addition, dissociation-induced (either mechanical or enzymatic) population and expression bias are also problematic. It is more feasible to isolate intact nuclei to be used as the starting material for most single-cell genomics applications.<\/p>\n<p>The detergent-free nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Intact nuclei can be isolated from the samples using proprietary spin-column-based technologies in less than 20 min without using tissue homogenizer and any detergents. The traditional method for nuclei isolation involves the use of non-ionic detergent, which has a tendency to cause unwanted nuclear aggregation. It is unclear why some nuclei are aggregated while others are not. It is also unknown whether non-aggregated nuclei are an unbiased representation of the whole nuclei population. Detergents also have the potential to damage the nuclear envelope resulting in leakage of nuclear matrix materials. For some cell\/tissue types, nuclear envelop proteins could also be stripped off by the detergents. Minute<sup>TM\u00a0<\/sup>Detergent-Free Nuclei Isolation Kit provides a whole new way for nuclei isolation in contrast to traditional methods.<\/p>\n<p><strong>How it works:<\/strong>\u00a0cells\/tissues are first sensitized by buffer A before passing through the proprietary filter in a zigzag manner when high-speed centrifugal force is applied. The cells are ruptured when passing through the filter leaving intact native nuclei in the flow-through. The nuclei are separated from other small cell debris by low-speed centrifugation using the proprietary buffer B. The native and intact nuclei isolated can be used for a variety of downstream applications that include but not limited to: FACS analysis, single nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis, and\/or apoptosis research.<\/p>\n<p><em><strong>For storage and transportation of isolated single nuclei, the following buffer is recommended:<\/strong><\/em><\/p>\n<p><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-anti-clumping-nuclei-storage-buffer-10-ml?_pos=1&amp;_sid=8b67760c6&amp;_ss=r\" target=\"_blank\" rel=\"noopener\"><em>WA-014:\u00a0Minute\u2122 Anti-Clumping Nuclei Storage Buffer\u00a0<\/em><\/a><\/p>\n<p><em><strong>NOTE:<\/strong>\u00a0We also offer the following single nucleus isolation kits with a much cleaner background if\u00a0the presence of detergent is not a concern:<\/em><\/p>\n<p><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-tissues-cells-20-preps?_pos=1&amp;_sid=2bc6f4939&amp;_ss=r\" target=\"_blank\" rel=\"noopener\">SN-047:\u00a0Minute\u2122 Single Nucleus Isolation Kit for Tissues\/Cells<\/a><\/em><\/p>\n<p><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-single-nucleus-isolation-kit-for-neuronal-tissues-cells-20-preps?_pos=1&amp;_sid=1f449fc5a&amp;_ss=r\" target=\"_blank\" rel=\"noopener\">BN-020:\u00a0Minute\u2122 Single Nucleus Isolation Kit for Neuronal Tissues\/Cells<\/a><\/em><\/p>\n<p><em><a href=\"https:\/\/inventbiotech.com\/products\/minute%E2%84%A2-nuclei-and-cytosol-isolation-kit-for-adipose-tissues?_pos=1&amp;_sid=f91e6c921&amp;_ss=r\" target=\"_blank\" rel=\"noopener\">AN-029:\u00a0Minute\u2122 Nuclei and Cytosol Isolation Kit for Adipose Tissues\/Cultured Adipocytes<\/a><\/em><\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024-2.jpg\" \/><\/p>\n<p><strong><span>\u4f7f\u7528 NI-024 \u5f9e\u6591\u99ac\u9b5a\u8166\u7d44\u7e54\u4e2d\u5206\u96e2\u7d30\u80de\u6838\uff0c\u4e26\u7528 DAPI \u67d3\u8272\uff08\u5047\u8272\u70ba\u7da0\u8272\uff09\uff08\u7531 Sema Elif Eski\u3001Singh Lab\u3001Universit\u00e9 Libre de Bruxelles \u63d0\u4f9b\uff09\u3002<\/span><\/strong><\/p>\n<p><strong>Nuclei Isolated from zebrafish brain tissue Using NI-024 and stained with DAPI (False-coloured in green) (Courtesy Sema Elif Eski, Singh Lab, Universite\u0301 Libre de Bruxelles).<\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024-3.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/R-F_copy_large.jpg?v=1564769298\" alt=\"\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024-5.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024-6.jpg\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024-7.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024-8.webp\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Minute\u2122 \u7121\u53bb\u57a2\u5291\u55ae\u6838\u5206\u96e2\u8a66\u5291Kit includes:<\/strong><\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>Items<\/strong><\/td>\n<td><strong>Quantity<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Buffer A<\/td>\n<td>15 ml<\/td>\n<\/tr>\n<tr>\n<td>Buffer B<\/td>\n<td>30 ml<\/td>\n<\/tr>\n<tr>\n<td>Filter Cartridges<\/td>\n<td>20 units<\/td>\n<\/tr>\n<tr>\n<td>Collection Tubes<\/td>\n<td>20 units<\/td>\n<\/tr>\n<tr>\n<td>Pestles<\/td>\n<td>2 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E7%84%A1%E5%8E%BB%E5%9E%A2%E5%8A%91%E5%96%AE%E6%A0%B8%E5%88%86%E9%9B%A2%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FNI-024.webp\" width=\"350\" height=\"350\" \/><\/p>\n<p><strong><a href=\"https:\/\/inventbiotech.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%8E%9F%E5%BB%A0%E9%80%A3%E7%B5%9001-300x79.png\" alt=\"\u539f\u5ee0\u9023\u7d5001\" width=\"138\" height=\"36\" \/><\/a><\/strong><strong><a href=\"https:\/\/www.abmgood.com\/\"><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/a><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/strong><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u57fa\u65bc\u65cb\u8f49\u67f1(SPIN-COLUMN-BASED )\u7684\u86cb\u767d\u8cea\u63d0\u53d6\/\u7d30\u80de\u5206\u7d1a(CELL FRACTIONATION)<\/span><\/strong><\/span><\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-4356\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2015\/02\/Compined-F.gif\" alt=\"Compined-F\" width=\"446\" height=\"167\" \/><\/strong><\/p>\n<p>Invent Biotechnologies \u6210\u7acb\u65bc 2010 \u5e74\uff0c\u56e0\u9769\u65b0\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u800c\u4eab\u8b7d\u5168\u7403\u3002\u6211\u5011\u958b\u767c\u4e86\u57fa\u65bc\u65cb\u8f49\u67f1(spin column-based)\u7684\u5275\u65b0\u6280\u8853\uff0c\u7528\u65bc\u86cb\u767d\u8cea\u7814\u7a76( protein research)\uff0c\u53ef\u7c21\u5316\u548c\u589e\u5f37\u6a23\u54c1\u88fd\u5099\u3002\u9019\u6703\u5e36\u4f86\u66f4\u9806\u66a2\u7684\u5de5\u4f5c\u6d41\u7a0b\u548c\u59cb\u7d42\u53ef\u9760\u7684\u7d50\u679c\uff0c\u5f9e\u800c\u5e36\u4f86\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002\u9078\u64c7 Minute\u2122 \u8a66\u5291\u76d2\u9032\u884c\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2(cell fractionation)\u7684\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u76f8\u4fe1\uff0c\u4ed6\u5011\u7684\u6a23\u54c1\u6e96\u78ba\u5730\u4ee3\u8868\u4e86\u6240\u7814\u7a76\u7684\u7d30\u80de\u6216\u7d44\u7e54\u7684\u81ea\u7136\u7279\u5fb5\u3002\u9019\u4e9b\u5957\u4ef6\u53ea\u9700\u8981\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\uff0c\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u5be6\u9a57\u8b8a\u91cf\u4e26\u78ba\u4fdd\u9ad8\u8cea\u91cf\u7684\u6750\u6599\u4ee5\u7372\u5f97\u66f4\u53ef\u9760\u7684\u6578\u64da\u3002Minute\u2122 \u65b9\u6cd5\u7684\u6709\u6548\u6027\u5f97\u5230\u4e86\u8d8a\u4f86\u8d8a\u591a journals\u7684\u652f\u6301 (Nature, Science, and Cell.)\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #ff0000;\"><b>The Minute\u2122 \u7684\u512a\u9ede\uff1a<\/b><\/span><br \/>\n\u2022 \u8d85\u5feb\uff08\u5feb\u81f3 1 \u5206\u9418\uff09<br \/>\n\u2022 \u6a23\u672c\u91cf\u5c0f<br \/>\n\u2022 \u6700\u5c0f\u5316\u4ea4\u53c9\u6c61\u67d3<br \/>\n\u2022 \u5167\u6e90\u6027\u57fa\u7dda\u4e0d\u8b8a<br \/>\n\u2022 \u6240\u6709\u6d3b\u9ad4\u548c\u7d44\u7e54\u985e\u578b<\/p>\n<p><span style=\"color: #ff0000;\"><strong><span style=\"font-size: 12pt;\">The Minute\u2122 \u61c9\u7528\uff1a<\/span><\/strong><\/span><br \/>\n\u2022 \u86cb\u767d\u8cea\u904b\u8f38\/\u6613\u4f4d<br \/>\n\u2022 \u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528<br \/>\n\u2022 \u86cb\u767d\u8cea\u4fee\u98fe<br \/>\n\u2022 \u4fe1\u865f\u8f49\u5c0e<br \/>\n\u2022 sc\/snRNA-seq<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">Invent Minute\u2122 \u5fb9\u5e95\u6539\u8b8a\u86cb\u767d\u8cea\u6a23\u54c1\u88fd\u5099\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2<\/span><\/strong><\/p>\n<div class=\"rte\">\n<p>\u81ea 2010 \u5e74\u6210\u7acb\u4ee5\u4f86\uff0c\u6211\u5011\u7684\u9996\u8981\u76ee\u6a19\u662f\u5229\u7528\u6211\u5011\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u5275\u65b0\u6280\u8853\u5fb9\u5e95\u6539\u8b8a\u5168\u7403\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u5e02\u5834\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u8b93 Minute\u2122 \u8a66\u5291\u76d2\u6210\u70ba\u6a19\u6e96\u5be6\u9a57\u5ba4\u7a0b\u5e8f\uff0c\u53d6\u4ee3\u6240\u6709\u57fa\u65bc\u6eb6\u6db2\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u3002\u901a\u904e\u5229\u7528\u9019\u4e9b\u5957\u4ef6\uff0c\u7814\u7a76\u4eba\u54e1\u53ea\u9700\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\u5373\u53ef\u7372\u5f97\u4e00\u81f4\u7684\u7d50\u679c\u4e26\u7372\u5f97\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/5-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"491\" height=\"239\" \/><\/p>\n<p><span style=\"font-size: 12pt;\"><strong><span style=\"color: #800000;\">\u6539\u8b8a\u86cb\u767d\u8cea\u63d0\u53d6(Transforming protein extraction)<\/span><\/strong><\/span><\/p>\n<p>Minute\u2122 \u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1( simple spin column )\uff0c\u53ef\u6709\u6548\u6d88\u9664\u7d30\u80de\u6216\u7d44\u7e54\u88c2\u89e3\u904e\u7a0b(lysis)\u4e2d\u7684\u975e\u7cfb\u7d71\u6027\u86cb\u767d\u8cea\u640d\u5931(non-systematic protein loss)\u3002\u9019\u4ee3\u8868\u8457\u76f8\u5c0d\u65bc\u50b3\u7d71\u7684\u57fa\u65bc\u89e3\u6c7a\u65b9\u6848\u7684\u65b9\u6cd5\uff0c\u4f8b\u5982 RIPA \u7de9\u885d\u6db2(RIPA buffer)\u548c\u8d85\u8072\u8655\u7406(sonication)\u7684\u986f\u8457\u9032\u6b65\uff0c\u9019\u4e9b\u65b9\u6cd5\u4ee5\u5176\u53ef\u8b8a\u6027\u548c\u7121\u6cd5\u63d0\u4f9b\u5168\u9762\u7684\u86cb\u767d\u8cea\u8b5c\u800c\u805e\u540d\u3002\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\u7684 Minute\u2122 \u8a66\u5291\u76d2\u8207\u4f86\u81ea\u5404\u7a2e\u751f\u7269\u9ad4\u548c\u7d44\u7e54\u985e\u578b\u7684\u5c0f\u6a23\u672c\u91cf\u517c\u5bb9\uff0c\u70ba\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u4e86\u672a\u6539\u8b8a\u7684\u5167\u6e90\u86cb\u767d\u57fa\u7dda( endogenous proteins)\uff0c\u5f9e\u800c\u5be6\u73fe\u66f4\u7cbe\u78ba\u7684\u5b9a\u91cf\u548c\u5206\u6790\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-Biotechs-Minute-Kit-shows-a-more-complete-cell-lysis-compared-to-RIPA-buffer-in-which-a-pellet-remains-leading-to-an-alteration-of-the-protein-profile.jpg\" width=\"374\" height=\"481\" \/><\/p>\n<p>Invent Biotech&#8217;s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/3-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"488\" height=\"243\" \/><\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">\u6539\u9032\u7d30\u80de\u5206\u7d1a\u5206\u96e2(Revamping cell fractionation)<\/span><\/strong><\/p>\n<p>\u7531\u65bc\u7f3a\u4e4f\u7c21\u55ae\u7684\u5be6\u9a57\u65b9\u6848\uff0c\u50b3\u7d71\u7684\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u65b9\u6cd5\u9577\u671f\u4ee5\u4f86\u4e00\u76f4\u53d7\u5230\u7528\u6236\u504f\u5dee\u548c\u7d50\u679c\u4e0d\u4e00\u81f4\u7684\u5f71\u97ff\u3002 Minute\u2122 \u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2\u5fb9\u5e95\u6539\u8b8a\u4e86\u9019\u4e00\u904e\u7a0b\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u5728 90 \u5206\u9418\u5167\u7372\u5f97\u9ad8\u7d14\u5ea6\u7684\u7d30\u80de\u5668\u7fa4\uff0c\u4e26\u4e14\u4f7f\u7528\u6700\u5c11\u7684\u6d17\u6ecc\u5291\u3002\u901a\u904e\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1\uff0c\u9019\u4e9b\u8a66\u5291\u76d2\u7121\u9700\u5747\u8cea\u5316(homogenization)\u3001\u5bc6\u5ea6\u68af\u5ea6\u5206\u96e2(density gradient separation)\u548c\u8d85\u901f\u96e2\u5fc3\u7b49\u50b3\u7d71\u6280\u8853( ultra-centrifugation)\uff0c\u5f9e\u800c\u6e1b\u5c11\u4e86\u5be6\u9a57\u8b8a\u91cf\u7684\u6578\u91cf\u3002\u9019\u53ef\u4ee5\u9632\u6b62\u7d30\u80de\u5668\u640d\u50b7( organelle damage)\u4e26\u7522\u751f\u66f4\u4e00\u81f4\u548c\u751f\u7406\u76f8\u95dc\u7684\u7d50\u679c\u3002\u6191\u85c9\u591a\u7a2e Minute\u2122 \u8a66\u5291\u76d2\u53ef\u6eff\u8db3\u4e0d\u540c\u7684\u7d30\u80de\u5206\u7d1a(cell fractionation)\u5206\u96e2\u8981\u6c42\uff0c\u6211\u5011\u6b63\u5728\u63a8\u52d5\u86cb\u767d\u8cea\u7d44\u7814\u7a76\u7684\u8f49\u578b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/2-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"480\" height=\"238\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u652f\u6301\u6240\u6709\u7814\u7a76\u5b78\u79d1(Supporting all research disciplines)<\/strong><\/span><\/p>\n<p>Minute\u2122 \u8a66\u5291\u76d2\u65e8\u5728\u7121\u7e2b\u96c6\u6210\u5230\u73fe\u6709\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u63d0\u4f9b\u5feb\u901f\u4e14\u4e00\u81f4\u7684\u6027\u80fd\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u7d50\u679c\u3002\u7136\u800c\uff0c\u4e0d\u8981\u53ea\u76f8\u4fe1\u6211\u5011\u7684\u8a71\u2014\u2014\u6211\u5011\u7684\u7522\u54c1\u5df2\u5728\u79d1\u5b78\u6587\u737b\u4e2d\u88ab\u5ee3\u6cdb\u5f15\u7528\uff0c\u9019\u8868\u660e\u7814\u7a76\u754c\u5c0d\u7c21\u5316\u4e14\u53ef\u9760\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u7684\u9700\u6c42\u4e0d\u65b7\u589e\u9577\u3002\u7acb\u5373\u5207\u63db\u5230 Minute\u2122\uff0c\u9ad4\u9a57\u6211\u5011\u6280\u8853\u7684\u512a\u52e2\uff0c\u7121\u8ad6\u60a8\u7684\u7814\u7a76\u9818\u57df\u5982\u4f55\u3002<\/p>\n<table style=\"height: 2673px;\" width=\"995\">\n<tbody>\n<tr>\n<td width=\"64\">SD-001\/SN-002<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-003<\/td>\n<td>Minute <sup>TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-006<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005-p<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MP-007<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-038<\/td>\n<td>Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SD-008\/SN-009<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-010<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CP-011<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SB-004<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-012<\/td>\n<td>Minute <sup>TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NE-013<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HP-014<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YT-015<\/td>\n<td width=\"667\">Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YD-016<\/td>\n<td width=\"667\">Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YM-017<\/td>\n<td>Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-018<\/td>\n<td width=\"667\">Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-019<\/td>\n<td width=\"667\">Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HD-021<\/td>\n<td width=\"667\">MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AT-022<\/td>\n<td width=\"667\">MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AF-023<\/td>\n<td>MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NI-024<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">BN-020<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-047<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AN-029<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">FE-025<\/td>\n<td>Minute<sup>TM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MS-026<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Mass Spectrometry (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">EI-027<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ED-028<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Endosome Isolation and Cell Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SE-030<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CS-031<\/td>\n<td>Minute<sup>TM<\/sup> Cell Suspension Isolation from Fresh\/Frozen Tissues (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NT-032<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NM-033<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LY-034<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-035<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> High Fidelity ER Isolation Kit for Liver Tissue (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-036<\/td>\n<td>Minute<sup>TM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">GO-037<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-039<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-042<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PT-040<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-041<\/td>\n<td>Minute<sup>TM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PF-045<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SV-043<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ML-044<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">RD-046<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Rubisco Depletion Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PR-048<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant ER Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PG-049<\/td>\n<td>Minute<sup>TM<\/sup> Plant Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PE-050<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Endosome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PL-051<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Plant Lipid Raft Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SY-052<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Synaptosome Isolation Kit (50 preps) NEW!<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-01-SK<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-02-BT<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-03-BV<\/td>\n<td>Minute<sup>TM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-04-TD<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-05-IM<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-06-MS<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SA-07-IS<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-002<\/td>\n<td width=\"667\">Minute\u2122 Ab Enhancer (30 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-003<\/td>\n<td width=\"667\">Minute\u2122 WB Stripping Solution (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-004<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Ponceau S Stain (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Peroxidase Suppressor (30 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-006<\/td>\n<td>Minute <sup>TM<\/sup> High Efficiency Protein Precipitation kit<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-007<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> RBC Lysis Buffer (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-008<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> WB Blocking Solution (250 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-009<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Denaturing Protein Solubilization Reagent (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-010<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Non-Denatured Protein Solubilization Reagent (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-011<\/td>\n<td>Minute<sup>TM<\/sup> Protein Solubilization Reagent for MS (2 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-012<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> SDS-Remover (10 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-013<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Albumin Depletion Reagent for Plasma and Serum (20 ml)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">WA-014<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Anti-Clumping Nuclei Storage Buffer NEW!<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-Rapid Protein Extraction Kits Minute\u2122\u86cb\u767d\u8403\u53d6\u5957 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5889],"tags":[6259],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44895"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44895"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44895\/revisions"}],"predecessor-version":[{"id":44896,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44895\/revisions\/44896"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44895"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44895"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44895"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}