{"id":44835,"date":"2023-08-04T13:46:40","date_gmt":"2023-08-04T05:46:40","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44835"},"modified":"2023-10-22T12:25:21","modified_gmt":"2023-10-22T04:25:21","slug":"mouse-anti-human-cd107a-unlb-h4a3%e6%8a%97%e9%ab%94-lamp1-southernbiotech%e8%b2%a8%e8%99%9f9835-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44835","title":{"rendered":"Mouse Anti-Human CD107a-UNLB (H4A3)\u6297\u9ad4 | LAMP1 Southernbiotech\u8ca8\u865f9835-01"},"content":{"rendered":"

Mouse Anti-Human CD107a-UNLB (H4A3)\u6297\u9ad4<\/strong> | LAMP1 Southernbiotech<\/strong>\u8ca8\u865f<\/strong>9835-01<\/strong><\/a><\/span><\/p>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\u3002\u7269\u7a2e\u7a2e\u985e\u9f4a\u5168\uff0c\u5305\u62ec\u6709Bovine\u3001Canine\u3001Chicken\u3001Cynomolgus\u3001Donkey\u3001Equine\u3001Feline\u3001Goat\u3001Guinea Pig\u3001Hamster\u3001Human\u3001Monkey\u3001Mouse\u3001Porcine\u3001Rabbit\u3001Rat\u3001Rhesus\u3001Sheep\u3001Turkey\u3002\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n

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CD107a\uff0c\u4e5f\u7a31\u70ba\u6eb6\u9176\u9ad4\u76f8\u95dc\u819c\u86cb\u767d 1 (lysosomal-associated membrane protein 1, LAMP-1)\uff0c\u662f\u4e00\u7a2e\u9ad8\u5ea6\u7cd6\u57fa\u5316\u7684 I \u578b\u8de8\u819c\u86cb\u767d(glycosylated type I transmembrane protein)\uff0c\u69cb\u6210\u6eb6\u9176\u9ad4\u819c( lysosomal membranes)\u4e0a\u7684\u4e3b\u8981\u553e\u6db2\u9178\u7cd6\u86cb\u767d(sialoglycoproteins)\u3002\u5b83\u662f Galaptin\uff08\u4e00\u7a2e\u5b58\u5728\u65bc\u7d30\u80de\u5916\u57fa\u8cea\u4e2d\u7684 S \u578b\u51dd\u96c6\u7d20\uff09\u7684\u914d\u9ad4\uff0c\u901a\u904e\u8b58\u5225\u4e59\u91af\u57fa\u4e73\u7cd6\u80fa\u5be1\u7cd6\u93c8\uff0c\u4e26\u4e14\u662f E-\u9078\u64c7\u7d20\u4ecb\u5c0e( E-selectin-mediated )\u7684\u7d30\u80de\u7c98\u9644(cell adhesion)\u7684\u914d\u9ad4\u3002 CD107a \u7531\u6d3b\u5316\u7684 T \u7d30\u80de( T cells)\u3001\u5de8\u566c\u7d30\u80de(macrophages)\u3001\u6a39\u7a81\u72c0\u7d30\u80de(dendritic cells)\u3001\u6d3b\u5316\u7684\u8840\u5c0f\u677f( activated platelets)\u3001\u6241\u6843\u9ad4\u4e0a\u76ae\u548c\u4e00\u4e9b\u816b\u7624\u7d30\u80de\u7cfb\uff08\u5305\u62ec U937 \u548c KG1a\uff09\u8868\u9054\u3002\u5b83\u4e5f\u662f\u4e00\u7a2e\u5ee3\u6cdb\u8868\u9054\u7684\u7d30\u80de\u5167\u6297\u539f\u3002 LAMP-1 (lysosomal-associated membrane protein 1, LAMP-1)\u53ef\u80fd\u901a\u904e\u5f62\u6210\u6eb6\u9176\u9ad4\u6c34\u89e3\u9176( lysosomal hydrolases)\u5c4f\u969c\u4f86\u4fdd\u8b77\u6eb6\u9176\u9ad4\u819c\u7684\u5167\u8868\u9762\u3002\u816b\u7624\u7d30\u80de\u7cfb\u8868\u9762\u4e0a CD107a \u548c CD107b \u7684\u4e0a\u8abf\u8207\u5176\u589e\u5f37\u7684\u8f49\u79fb\u6f5b\u529b\u76f8\u95dc\uff0c\u5176\u4e2d\u5b83\u5011\u53ef\u80fd\u589e\u52a0\u8207\u7d30\u80de\u5916\u57fa\u8cea(extracellular matrix)\u548c\u5167\u76ae( endothelium)\u7684\u7c98\u9644\u3002<\/p>\n

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\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

Mouse Anti-Human CD107a (LAMP1)<\/strong>\u6297\u9ad4\u7522\u54c1\u8cc7\u6599<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/th>\nH4A3<\/td>\n<\/tr>\n
Isotype<\/th>\nMouse (BALB\/c) IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/th>\nMouse IgG1-UNLB (15H6)<\/a><\/td>\n<\/tr>\n
Specificity<\/th>\nHuman\/Rhesus\/African Green Monkey\/Pigtail Macaque\/Sooty Mangabey CD107a<\/td>\n<\/tr>\n
Alternative Names<\/th>\nLAMP-1, lysosome-associated membrane protein 1<\/td>\n<\/tr>\n
Description<\/th>\nCD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is expressed by activated T cells, macrophages, dendritic cells, activated platelets, tonsillar epithelium, and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential where they may increase adhesion to extracellular matrix and endothelium.<\/td>\n<\/tr>\n
Immunogen<\/th>\nHuman adherent peripheral blood cells<\/td>\n<\/tr>\n
Conjugate<\/th>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/th>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Clonality<\/th>\nMonoclonal<\/td>\n<\/tr>\n
Concentration<\/th>\n0.1 mg\/mL<\/td>\n<\/tr>\n
Volume<\/th>\n1.0 mL<\/td>\n<\/tr>\n
Recommended Storage<\/th>\n2-8\u00b0C<\/td>\n<\/tr>\n
Applications<\/th>\nFlow Cytometry \u2013 Quality tested\u00a01,12-19,21,22<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a02<\/sup>
\nImmunohistochemistry-Paraffin Sections \u2013 Reported in literature\u00a03,4<\/sup>
\nImmunocytochemistry \u2013 Reported in literature\u00a05-10,20<\/sup>
\nElectron Microscopy \u2013 Reported in literature\u00a01<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01,5,6<\/sup>
\nWestern Blot \u2013 Reported in literature\u00a011<\/sup>
\nPurification \u2013 Reported in literature\u00a01<\/sup><\/td>\n<\/tr>\n
RRID Number<\/th>\nAB_2797102<\/td>\n<\/tr>\n
Gene ID<\/th>\n3916<\/a>\u00a0(Human)
\n697331<\/a>\u00a0(Rhesus)
\n103214841<\/a>\u00a0(Green Monkey)
\n105485253<\/a>\u00a0(Pigtail Macaque)
\n105584952<\/a>\u00a0(Sooty Mangabey)<\/td>\n<\/tr>\n
Gene ID Symbol<\/th>\nLAMP1<\/a>\u00a0(Human)
\nLAMP1<\/a>\u00a0(Rhesus)
\nLAMP1<\/a>\u00a0(Green Monkey)
\nLAMP1<\/a>\u00a0(Pigtail Macaque)
\nLAMP1<\/a>\u00a0(Sooty Mangabey)<\/td>\n<\/tr>\n
Gene ID Aliases<\/th>\nCD107a; LAMPA; LGP120<\/td>\n<\/tr>\n
UniProt ID<\/th>\nP11279<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
UniProt Name<\/th>\nLAMP1_HUMAN<\/a>\u00a0(Human)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1\u00a0<\/span>Isotype control \u5206\u578b\u5c0d\u7167\u7d44<\/a> |\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/a> | \u6297\u9ad4\u5206\u578bELISA \u5957\u7d44<\/a> | anti-rabbit \u4e8c\u7d1a\u6297\u9ad4<\/a> | anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a> |<\/span><\/strong><\/p>\n

\"\u5f15\u7528\u6587\u737b_\u5de5\u4f5c\u5340\u57df<\/p>\n

1. Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, et al. Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem
\nBiophys. 1989;268:360-78. (Immunogen, FC, EM, IP, Purification)
\n2. Momma K, Noguchi S, Malicdan MC, Hayashi YK, Minami N, Kamakura K, et al. Rimmed vacuoles in Becker muscular dystrophy have similar features with inclusion
\nmyopathies. PLoS One. 2012;7:e52002. (IHC-FS)
\n3. Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, et al. Expression of lysosome-associated membrane proteins in human colorectal neoplasms and
\ninflammatory diseases. Am J Pathol. 2001;159:449-55. (IHC-PS)
\n4. Sarafian VS, Dikov DI. LAMPs and ABH histo-blood group antigens in granulation tissue. APMIS. 2007;115:701-6. (IHC-PS)
\n5. Hauck CR, Meyer TF. The lysosomal\/phagosomal membrane protein h-lamp-1 is a target of the IgA1 protease of Neisseria gonorrhoeae. FEBS Lett. 1997;405:86-90.
\n(ICC, IP)
\n6. Dell’Angelica EC, Aguilar RC, Wolins N, Hazelwood S, Gahl WA, Bonifacino JS. Molecular characterization of the protein encoded by the Hermansky-Pudlak syndrome
\ntype 1 gene. J Biol Chem. 2000;275:1300-6. (ICC, IP)
\n7. Val\u00e9s-G\u00f3mez M, Winterhalter A, Roda-Navarro P, Zimmermann A, Boyle L, Hengel H, et al. The human cytomegalovirus glycoprotein UL16 traffics through the plasma
\nmembrane and the nuclear envelope. Cell Microbiol. 2006;8:581-90. (ICC)
\n8. Val\u00e9s-G\u00f3mez M, Reyburn HT. Intracellular trafficking of the HCMV immunoevasin UL16 depends on elements present in both its cytoplasmic and transmembrane
\ndomains. J Mol Biol. 2006;363:908-17. (ICC)
\n9. Hanna MC, Blackstone C. Interaction of the SPG21 protein ACP33\/maspardin with the aldehyde dehydrogenase ALDH16A1. Neurogenetics. 2009;10:217-28. (ICC)
\n10. Al-Zeer MA, Al-Younes HM, Lauster D, Lubad MA, Meyer TF. Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate
\nbinding proteins. Autophagy. 2013;9:50-62. (ICC)
\n11. Armstrong A, Mattsson N, Appelqvist H, Janefjord C, Sandin L, Agholme L, et al. Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer’s disease.
\nNeuromolecular Med. 2014;16:150-60. (WB)
\n12. Hershkovitz O, Rosental B, Rosenberg LA, Navarro-Sanchez ME, Jivov S, Zilka A, et al. NKp44 receptor mediates interaction of the envelope glycoproteins from the West
\nNile and dengue viruses with NK cells. J Immunol. 2009;183:2610-21. (FC)
\n13. Kozako T, Yoshimitsu M, Fujiwara H, Masamoto I, Horai S, White Y, et al. PD-1\/PD-L1 expression in human T-cell leukemia virus type 1 carriers and adult T-cell
\nleukemia\/lymphoma patients. Leukemia. 2009;23:375-82. (FC)
\n14. Kozako T, Yoshimitsu M, Akimoto M, White Y, Matsushita K, Soeda S, et al. Programmed death-1 (PD-1)\/PD-1 ligand pathway-mediated immune responses against
\nhuman T-lymphotropic virus type 1 (HTLV-1) in HTLV-1-associated myelopathy\/tropical spastic paraparesis and carriers with autoimmune disorders. Hum Immunol.
\n2011;72:1001-6. (FC)
\n15. Rosental B, Brusilovsky M, Hadad U, Oz D, Appel MY, Afergan F, et al. Proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor
\nNKp44. J Immunol. 2011;187:5693-702. (FC)
\n16. Jaron-Mendelson M, Yossef R, Appel MY, Zilka A, Hadad U, Afergan F, et al. Dimerization of NKp46 receptor is essential for NKp46-mediated lysis: characterization of the
\ndimerization site by epitope mapping. J Immunol. 2012;188:6165-74. (FC)
\n17. Genesc\u00e0 M, Skinner PJ, Bost KM, Lu D, Wang Y, Rourke TL, et al. Protective attenuated lentivirus immunization induces SIV-specific T cells in the genital tract of rhesus
\nmonkeys. Mucosal Immunol. 2008;1:219-28. (FC, Rhesus Reactivity)
\n18. Magalhaes I, Vudattu NK, Ahmed RK, K\u00fchlmann-Berenzon S, Ngo Y, Sizemore DR, et al. High content cellular immune profiling reveals differences between rhesus
\nmonkeys and men. Immunology. 2010;131:128-40. (FC, Rhesus Reactivity)
\n19. Cicin-Sain L, Sylwester AW, Hagen SI, Siess DC, Currier N, Legasse AW, et al. Cytomegalovirus-specific T cell immunity is maintained in immunosenescent rhesus
\nmacaques. J Immunol. 2011;187:1722-32. (FC, Rhesus Reactivity)
\n20. Sauer J, Shannon JG, Howe D, Hayes SF, Swanson MS, Heinzen RA. Specificity of Legionella pneumophila and Coxiella burnetii vacuoles and versatility of Legionella
\npneumophila revealed by coinfection. Infect Immun. 2005;73:4494-504. (ICC, African Green Monkey Reactivity)
\n21. Jegaskanda S, Amarasena TH, Laurie KL, Tan H, Butler J, Parsons MS, et al. Standard trivalent influenza virus protein vaccination does not prime antibody-dependent
\ncellular cytotoxicity in macaques. J Virol. 2013;87:13706-18. (FC, Pigtail Macaque Reactivity)
\n22. Dunham R, Pagliardini P, Gordon S, Sumpter B, Engram J, Moanna A, et al. The AIDS resistance of naturally SIV-infected sooty mangabeys is independent of cellular
\nimmunity to the virus. Blood. 2006;108:209-17. (FC, Sooty Mangabey Reactivity)<\/p>\n","protected":false},"excerpt":{"rendered":"

Mouse Anti-Human CD107a-UNLB (H4A3)\u6297\u9ad4 | LAMP1 Southernb […]<\/p>\n","protected":false},"author":3,"featured_media":29304,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5381],"tags":[6197],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44835"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44835"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44835\/revisions"}],"predecessor-version":[{"id":44836,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44835\/revisions\/44836"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29304"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44835"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44835"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44835"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}