{"id":44803,"date":"2023-08-03T15:16:22","date_gmt":"2023-08-03T07:16:22","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44803"},"modified":"2023-10-22T12:25:47","modified_gmt":"2023-10-22T04:25:47","slug":"trakine-pro-%e6%b4%bb%e7%b4%b0%e8%83%9e-lysotracker%e8%a9%a6%e5%8a%91%e7%9b%92-%e7%b4%85%e8%89%b2%e8%9e%a2%e5%85%89-abbkine%e8%b2%a8%e8%99%9fktc4210","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44803","title":{"rendered":"TraKine\u2122 Pro \u6d3b\u7d30\u80de LysoTracker\u8a66\u5291\u76d2-\u7d05\u8272\u87a2\u5149 | Abbkine\u8ca8\u865fKTC4210"},"content":{"rendered":"

TraKine\u2122 Pro \u6d3b\u7d30\u80de LysoTracker\u8a66\u5291\u76d2\uff08\u7d05\u8272\u87a2\u5149\uff09 | TraKine\u2122 Pro Live-cell Lyso-traker kit Abbkine\u8ca8\u865fKTC4210<\/a><\/span><\/strong><\/span><\/p>\n

TraKine\u2122 Pro Live-cell Lyso-traker kit (Red Fluorescence)<\/span><\/p>\n

\u6eb6\u9176\u9ad4(Lysosomes )\u662f\u7d30\u80de\u5668\uff0c\u542b\u6709\u9178\u6027\u6c34\u89e3\u9176( acid hydrolase enzymes)\u4f86\u5206\u89e3\u5ee2\u7269( waste materials)\u548c\u7d30\u80de\u788e\u7247(cellular debris)\u3002\u6eb6\u9176\u9ad4\u6d88\u5316\u591a\u9918\u6216\u78e8\u640d\u7684\u7d30\u80de\u5668( organelles)\u3001\u98df\u7269\u9846\u7c92\u4ee5\u53ca\u541e\u566c\u7684\u75c5\u6bd2\u6216\u7d30\u83cc(bacteria)\u3002\u6eb6\u9176\u9ad4( lysosome)\u5468\u570d\u7684\u819c\u5141\u8a31\u6d88\u5316\u9176\u5728 pH 4.5 \u4e0b\u767c\u63ee\u4f5c\u7528\u3002\u8207\u5fae\u9e7c\u6027\u7d30\u80de\u8cea (pH 7.2) \u76f8\u6bd4\uff0c\u6eb6\u9176\u9ad4\u5167\u90e8\u5448\u9178\u6027 (pH 4.5-4.8)\u3002\u6eb6\u9176\u9ad4\u901a\u904e\u8cea\u5b50\u6cf5\u548c\u6c2f\u96e2\u5b50\u901a\u9053\u5c07\u8cea\u5b50\u5f9e\u7d30\u80de\u8cea\u6cf5\u51fa\u7a7f\u904e\u819c\u4f86\u7dad\u6301\u9019\u7a2e pH \u503c\u5dee\u7570\u3002<\/p>\n

<\/p>\n

TraKine\u2122 Pro \u662f\u4e00\u7cfb\u5217\u9577\u671f\u8d85\u5206\u8fa8\u7387\u7d30\u80de\u67d3\u8272\u6210\u50cf\u7522\u54c1\u7d44\u5408\uff0c\u7528\u65bc\u6a19\u8a18\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de\u7684\u4e9e\u7d30\u80de\u7d50\u69cb\u3002TraKine\u2122 LysoTracker\u512a\u5316\u7684\u67d3\u8272\u65b9\u6848\uff0c\u7528\u65bc\u6a19\u8a18\u54fa\u4e73\u52d5\u7269\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de\u4e2d\u7684\u6eb6\u9176\u9ad4\u3002\u8a72\u7522\u54c1\u5df2\u5728 U2OS\u3001Hela\u3001COS-7 \u548c ARPE \u7d30\u80de\u682a\u4e2d\u9032\u884c\u6e2c\u8a66\u3002\u5982\u679c\u6a23\u54c1\u985e\u578b\u4e0d\u5305\u542b\u5728\u4e0a\u8ff0\u7d30\u80de\u7cfb\u4e2d\uff0c\u6211\u5011\u53ef\u4ee5\u63d0\u4f9b\u91dd\u5c0d\u7279\u5b9a\u7d30\u80de\u682a\u7684\u5be6\u9a57\u670d\u52d9\u3002\u7279\u5225\u9069\u5408\u5171\u7126\u548c\u9577\u671f\u8d85\u5206\u8fa8\u7387\u6210\u50cf\uff08\u4f8b\u5982SIM\u3001TIRF\u3001STORM \u548cPALM\uff09\u3002\u5c08\u6709\u7684LysRed\u2122\uff08Ex\/Em = 650\/665nm\uff09- \u9ad8\u7279\u7570\u6027\u3001\u4f4e\u80cc\u666f\u548c\u51fa\u8272\u7684\u5149\u7a69\u5b9a\u6027\u3002\u4f4e\u6c34\u5e73\u7684\u7d30\u80de\u6bd2\u6027\u3002<\/p>\n

<\/p>\n

\u5716 1. \u5e02\u552eLyso Tracker\u3002Fig.1.Long time series images (a, c) and corresponding fluorescence bleaching curves (b, d) were obtained by using HiS SIM Abbkine TraKine\u2122 Pro Live-cell Lyso-traker kit (Red Fluorescence) and Spining disk confocal microscopy of Tubulin Green and commercially available Lyso Tracker on U-2 OS cells, respectively. Scale bars: 5 \u03bcM.<\/p>\n

<\/p>\n

Fig.2.Confocal imaging results of U2OS cells using TraKine\u2122 Pro Live-cell Lyso-traker kit (Red Fluorescence)<\/p>\n

<\/p>\n\n\n\n\n\n\n\n
Kit components<\/td>\n\u2022Lyso Red (200 \u03bcM)<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Optimized staining protocol for labeling Lysosome in mammalian living and fixed cells. The product has been tested in U2OS, Hela, COS-7 and ARPE cell lines. If the sample type is not included in the above cell lines, we can provide experimental services for specific cell lines.
\n\u2022 Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, TIRF, STORM and PALM).
\n\u2022 Proprietary LysRed\u2122 (Ex\/Em = 650\/665nm)-high specificity, low background and excellent photostability.
\n\u2022 Low levels of cytotoxicity.<\/td>\n<\/tr>\n
Usage notes<\/td>\nMake sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.<\/td>\n<\/tr>\n
Storage instructions<\/td>\nRefer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.<\/td>\n<\/tr>\n
Shipping<\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u9810\u67d3protein marker<\/a>\u00a0|\u00a0\u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291\u00a0<\/a>\u00a0|\u00a0\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>|\u00a0Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>|\u00a0\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/span><\/p>\n

Abbkine\u63d0\u4f9b\u4e00\u7cfb\u5217\u9ad8\u5ea6\u7279\u7570\u6027\u55ae\u514b\u9686\u548c\u591a\u514b\u9686\u6297\u9ad4\uff0ctag antibody\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\u5982 His tag\u3001GST tag\u3001S tag\u3001HA tag\u3001DDDDK tag\u3001Myc tag\u3001MBP tag\u548c mCherry tag\u7b49\u55ae\/\u591a\u682aTag\u6297\u9ad4\u3002\u53e6\u5177\u751f\u7269\u7d20(Biotin)\u3001HRP\u3001AbFluor\u2122 \u67d3\u6599\u3001\u74ca\u8102\u7cd6(agarose)\u548c\u78c1\u73e0\u5076\u806f\u7269(magnetic beads)\u6a19\u8a8c\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat.No<\/td>\nName<\/td>\nSize1<\/td>\n<\/tr>\n
KTD104-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Magnetic Beads)<\/td>\n20T<\/td>\n<\/tr>\n
KTD105-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Agarose)<\/td>\n20T<\/td>\n<\/tr>\n
A25012<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25022<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25112<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25122<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25222<\/td>\nIPKine\u2122 HRP, Goat Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
KTI2014<\/td>\nIPKine\u2122 Anti-DDDDK Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2024<\/td>\nIPKine\u2122 Anti-GFP Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2034<\/td>\nIPKine\u2122 Anti-GST Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2044<\/td>\nIPKine\u2122 Anti-HA Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2064<\/td>\nIPKine\u2122 Anti-Myc Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
ABT2013<\/td>\nAgarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2014<\/td>\nMagnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2024<\/td>\nMagnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2023<\/td>\nAgarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2043<\/td>\nAgarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2044<\/td>\nMagnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2053<\/td>\nAgarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2054<\/td>\nMagnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2063<\/td>\nAgarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2064<\/td>\nMagnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2173<\/td>\nAgarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2174<\/td>\nMagnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2010<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2011<\/td>\nAnti-DDDDK Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2015<\/td>\nHRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2020<\/td>\nAnti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2025<\/td>\nHRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2021<\/td>\nAnti-GFP Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2030<\/td>\nAnti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2035<\/td>\nHRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2040<\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2041<\/td>\nAnti-HA Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2045<\/td>\nHRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2050<\/td>\nAnti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2051<\/td>\nAnti-His Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2055<\/td>\nHRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2060<\/td>\nAnti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2061<\/td>\nAnti-Myc Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2065<\/td>\nHRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2170<\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2070<\/td>\nAnti-MBP Tag Mouse Monoclonal Antibody (9Y5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2080<\/td>\nAnti-mCherry Tag Mouse Monoclonal Antibody (9D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2090<\/td>\nAnti-E2 Tag Mouse Monoclonal Antibody (12T4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2100<\/td>\nAnti-HSV Tag Mouse Monoclonal Antibody (16T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2110<\/td>\nAnti-KT3 Tag Mouse Monoclonal Antibody (14D8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2120<\/td>\nAnti-RFP Tag Mouse Monoclonal Antibody (9D1)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2130<\/td>\nAnti-S Tag Mouse Monoclonal Antibody (9T10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2150<\/td>\nAnti-T7 Tag Mouse Monoclonal Antibody (6D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2160<\/td>\nAnti-Trx Tag Mouse Monoclonal Antibody (14D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2180<\/td>\nAnti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2190<\/td>\nAnti-CBP Tag Monoclonal Antibody (12H5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2200<\/td>\nAnti-TAP Tag Monoclonal Antibody (4H2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2210<\/td>\nAnti-Avi-Tag Monoclonal Antibody (5G11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2220<\/td>\nAnti-SRT-Tag Monoclonal Antibody (11G3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2230<\/td>\nAnti-Strep-Tag II Monoclonal Antibody (8C12)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2240<\/td>\nAnti-mStrawberry Monoclonal Antibody (4C9)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2250<\/td>\nAnti-EYFP-Tag Monoclonal Antibody (10T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2260<\/td>\nAnti-mOrange Monoclonal Antibody (9A10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2270<\/td>\nAnti-AmCyan Monoclonal Antibody (8T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2280<\/td>\nAnti-ECFP-Tag Monoclonal Antibody (6B11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2290<\/td>\nAnti-EBFP Monoclonal Antibody (8B5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2300<\/td>\nAnti-Nano-Tag9 Monoclonal Antibody (11T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

TraKine\u2122 Pro \u6d3b\u7d30\u80de LysoTracker\u8a66\u5291\u76d2\uff08\u7d05\u8272\u87a2\u5149\uff09 | TraKine\u2122 Pro Li […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[6199],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44803"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44803"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44803\/revisions"}],"predecessor-version":[{"id":44809,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44803\/revisions\/44809"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44803"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44803"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44803"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}