{"id":44569,"date":"2023-07-28T17:51:24","date_gmt":"2023-07-28T09:51:24","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44569"},"modified":"2023-08-30T11:32:59","modified_gmt":"2023-08-30T03:32:59","slug":"%e7%a5%9e%e7%b6%93%e9%86%af%e8%83%ba%e9%85%b6%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-neuraminidase-assay-kit-%e8%b2%a8%e8%99%9fabnova-ka1633","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44569","title":{"rendered":"\u795e\u7d93\u91af\u80fa\u9176\u5206\u6790\u8a66\u5291\u76d2| Neuraminidase Assay Kit \u8ca8\u865fAbnova\u2122\u00a0 KA1633"},"content":{"rendered":"<p><span style=\"color: #33cccc; font-size: 12pt;\"><strong><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=44569&amp;preview=true\"><span style=\"font-size: 12pt;\">\u795e\u7d93\u91af\u80fa\u9176\u5206\u6790\u8a66\u5291\u76d2| Neuraminidase Assay Kit \u8ca8\u865fAbnova\u2122\u00a0 KA1633<\/span><\/a><\/strong><\/span><\/p>\n<p>Neuraminidase Assay Kit \u9748\u654f\u5ea6Colorimetric Assay: 0.1 to 10 U\/L, Fluorimetric Assay: 0.01 to 2 U\/L<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/abnova.png\" width=\"628\" height=\"82\" \/><\/p>\n<p>\u795e\u7d93\u91af\u80fa\u9176\uff08NEURAMINDASE\u4e5f\u7a31\u70ba\u553e\u6db2\u9178\u9176\uff09\u662f\u4e00\u7a2e\u6c34\u89e3\u672b\u7aef\u553e\u6db2\u9178\u6b98\u57fa(terminal sialic acid residues )\u7684\u9176\u3002\u591a\u91a3\u93c8(poly-saccharide chains)\u3002\u5b83\u4e3b\u8981\u5728\u7d30\u83cc(bacteria )\u548c\u75c5\u6bd2( viruses)\u7b49\u5fae\u751f\u7269\u4e2d\u8868\u9054\u3002\u795e\u7d93\u91af\u80fa\u9176\uff08NEURAMINDASE\u4e5f\u7a31\u70ba\u553e\u6db2\u9178\u9176\uff09\u5c0d\u553e\u6db2\u9178\u6b98\u57fa\u7684\u5207\u5272\u88ab\u8a8d\u70ba\u5728\u6d41\u611f\u611f\u67d3\u4e2d\u767c\u63ee\u591a\u7a2e\u4f5c\u7528\u75c5\u6bd2\u3002\u5b83\u88ab\u8a8d\u70ba\u6709\u52a9\u65bc\u6ef2\u900f\u7c98\u819c\u5167\u5c64\u3001\u4fb5\u5165\u9776\u7d30\u80de\u3001elution\u4f86\u81ea\u53d7\u611f\u67d3\u7d30\u80de\u7684\u5b50\u4ee3\u75c5\u6bd2\uff0c\u4e26\u9632\u6b62\u81ea\u6211\u805a\u96c6(self-aggregation)\u3002\u56e0\u6b64\uff0c\u795e\u7d93\u91af\u80fa\u9176\uff08NEURAMINDASE\u4e5f\u7a31\u70ba\u553e\u6db2\u9178\u9176\uff09\u662f\u4e00\u7a2e\u6d41\u611f\u85e5\u7269\u958b\u767c\u7684\u91cd\u8981\u76ee\u6a19\u4ee5\u53ca\u7c21\u55ae\u3001\u76f4\u63a5\u548c\u81ea\u52d5\u5316\u7684\u7a0b\u5e8f\u6e2c\u91cf\u795e\u7d93\u6c28\u9178\u9176\u6d3b\u6027(neuraminidase activity)\u5728\u7814\u7a76\u548c\u85e5\u7269\u767c\u73fe\u4e2d\u6709\u8457\u5ee3\u6cdb\u7684\u61c9\u7528\u3002<\/p>\n<p><span style=\"font-size: 12pt;\"><strong><span style=\"color: #993366;\">\u6e2c\u5b9a\u539f\u7406 (Principle of the Assay)<\/span><\/strong><\/span><br \/>\n\u795e\u7d93\u6c28\u9178\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2(The Neuraminidase Assay Kit )\u53ef\u4e00\u6b65\u6e2c\u91cf\u795e\u7d93\u6c28\u9178\u9176\u91cb\u653e\u7684\u553e\u6db2\u9178(sialic acid)\u3002\u8b8a\u5316\u5728\u53cd\u61c9\u7522\u7269\u5728 570 nm \u8655\u7684\u984f\u8272\u5f37\u5ea6\u6216 \u03bbem\/ex = 585\/530 nm \u8655\u7684\u87a2\u5149\u5f37\u5ea6\u53ef\u76f4\u63a5\u8207\u6a23\u54c1\u4e2d\u7684\u795e\u7d93\u6c28\u9178\u9176\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/2-%E7%A5%9E%E7%B6%93%E9%86%AF%E8%83%BA%E9%85%B6%E5%88%86%E6%9E%90%E8%A9%A6%E5%8A%91%E7%9B%92Neuraminidase-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA1633.png\" width=\"250\" height=\"270\" \/><\/p>\n<p>The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.<\/p>\n<div id=\"0\">\n<p><span style=\"font-size: 12pt; color: #800000;\"><b>Specification<\/b><\/span><\/p>\n<\/div>\n<div id=\"10000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Product Description:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Neuraminidase Assay Kit is a quantitative colorimetric\/fluorimetric determination of neuraminidase activity.<\/li>\n<\/ul>\n<\/div>\n<div id=\"10100\">\n<ul>\n<li class=\"black12 sub_title\"><b>Suitable Sample:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Biological Sample<\/li>\n<\/ul>\n<\/div>\n<div id=\"10200\">\n<ul>\n<li class=\"black12 sub_title\"><b>Sample Volume:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">20 uL<\/li>\n<\/ul>\n<\/div>\n<div id=\"10400\">\n<ul>\n<li class=\"black12 sub_title\"><b>Detection Method:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Colorimetric, Fluorometric<\/li>\n<\/ul>\n<\/div>\n<div id=\"10450\">\n<ul>\n<li class=\"black12 sub_title\"><b>Assay Type:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Quantitative<\/li>\n<\/ul>\n<\/div>\n<div id=\"20000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Calibration Range:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Colorimetric Assay: 0.1 to 10 U\/L, Fluorimetric Assay: 0.01 to 2 U\/L<\/li>\n<\/ul>\n<\/div>\n<div id=\"260100\">\n<ul>\n<li class=\"black12 sub_title\"><b>Regulation Status:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">For research use only (RUO)<\/li>\n<\/ul>\n<p><a href=\" http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/1-\u795e\u7d93\u91af\u80fa\u9176\u5206\u6790\u8a66\u5291\u76d2-Neuraminidase-Assay-Kit-\u8ca8\u865fAbnova\u2122-KA1633.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">Colorimetric Procedure<\/span><\/strong><\/span><br \/>\nNote: SH-group containing reagents (e.g. mercaptoethanol, DTT) may interfere with this assay and should be avoided in sample preparation.<br \/>\n1. Equilibrate all components to desired reaction temperature (i.e 37\u00b0C). Prepare a 400 \u03bcM Standard Premix by mixing 20 \u03bcL of the 10 mM Standard and 480 \u03bcL dH2O. Dilute Standard in distilled water as follows. Transfer 20 \u03bcL standards into separate wells of a clear flat-bottom 96-well plate.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/3-%E7%A5%9E%E7%B6%93%E9%86%AF%E8%83%BA%E9%85%B6%E5%88%86%E6%9E%90%E8%A9%A6%E5%8A%91%E7%9B%92-Neuraminidase-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA1633.png\" \/><br \/>\n2. Transfer 20 \u03bcL of each sample into two separate wells of the same plate. One well will be used for the sample activity and one for the sample blank.<br \/>\n3. Immediately prior to starting the reaction, prepare enough Working Reagent (WR) for all sample and standard wells by mixing per reaction tube: 30 \u03bcL Assay Buffer, 55 \u03bcL Substrate, 1 \u03bcL Cofactors, 1 \u03bcL Enzyme and 0.5 \u03bcL Dye Reagent. For the sample blank wells, substitute 55 \u03bcL Assay Buffer for the 55 \u03bcL Substrate. Add 80 \u03bcL of the appropriate WR to each well.<br \/>\n4. Incubate the reaction plate protected from light at 37\u00b0C (or desired temperature) for 20 min. Measure the OD at 570 nm (OD20min). Incubate reaction plate for a further 30 min, again protected from light and at 37\u00b0C (or desired temperature). Measure the OD (OD50min)<\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">Fluorimetric Procedure<\/span><\/strong><\/span><br \/>\n1. Dilute the Standards prepared in Colorimetric Procedure 1:5 in H2O. Transfer 20 \u03bcL standards into<br \/>\nseparate wells of a black 96-well plate.<br \/>\n2. Transfer 20 \u03bcL of each sample into two separate wells of the same plate. One well will be used for the<br \/>\nsample activity and one for the sample blank.<br \/>\n3. Add 80 \u03bcL of appropriate Working Reagent (see Colorimetric Procedure) to each well. Tap plate to mix.<br \/>\n4. Incubate the reaction plate protected from light at 37\u00b0C (or desired temperature) for 20 min. Measure the<br \/>\nF (\u03bbex\/em = 530\/570 nm) (F20min). Incubate reaction plate for a further 30 min, again protected from light<br \/>\nand at 37\u00b0C (or desired temperature). Measure the F (F50min).<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/4-%E7%A5%9E%E7%B6%93%E9%86%AF%E8%83%BA%E9%85%B6%E5%88%86%E6%9E%90%E8%A9%A6%E5%8A%91%E7%9B%92-Neuraminidase-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA1633.png\" \/><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u795e\u7d93\u91af\u80fa\u9176\u5206\u6790\u8a66\u5291\u76d2| Neuraminidase Assay Kit \u8ca8\u865fAbnova\u2122\u00a0 KA1633 N [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6083],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44569"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44569"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44569\/revisions"}],"predecessor-version":[{"id":45450,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44569\/revisions\/45450"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44569"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44569"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44569"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}