{"id":44442,"date":"2023-07-27T11:54:06","date_gmt":"2023-07-27T03:54:06","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44442"},"modified":"2023-08-30T11:33:42","modified_gmt":"2023-08-30T03:33:42","slug":"%e7%a3%b7%e9%85%b8%e9%b9%bd%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-phosphate-assay-kit-%e8%b2%a8%e8%99%9fabnova-ka6676","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=44442","title":{"rendered":"\u78f7\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2| Phosphate Assay Kit\u00a0 \u8ca8\u865fAbnova\u2122 KA6676"},"content":{"rendered":"<p><span style=\"color: #33cccc; font-size: 12pt;\"><strong><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=44442\"><span style=\"font-size: 12pt;\">\u78f7\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2| Phosphate Assay Kit\u00a0 \u8ca8\u865fAbnova\u2122 KA6676<\/span><\/a><\/strong><\/span><\/p>\n<p>\u78f7\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u4f7f\u7528ELISA\u5fae\u91cf\u6ef4\u5b9a\u677f(microtiter plates0 \u63d0\u4f9b1000\u6b21\u6e2c\u5b9a\uff0c\u6216\u4f7f\u7528384well\u9032\u884c2000\u6b21\u6e2c\u5b9a)<\/p>\n<p>Phosphate Assay Kit (Colorimetric, 600-660nm)<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/abnova.png\" width=\"628\" height=\"82\" \/><\/p>\n<p>\u78f7\u9178\u6839\u6aa2\u6e2c\u8a66\u5291\uff0c\u70ba\u6aa2\u6e2c\u7121\u6a5f\u78f7\u9178\u6839\uff08Pi\uff09\u4e4b\u542b\u91cf\uff1b\u4f7f\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\u3002\u53ef\u7528\u65bc\u6e2c\u91cf\u7522\u751fPi\u7684\u4efb\u4f55\u9176\uff0c\u5305\u62ecATPases\u3001GTPases, phosphatases\u3001\u71b1\u4f11\u514b\u86cb\u767d\u53caDNA\u89e3\u65cb\u86cb\u767d\u3002<\/p>\n<p>\u78f7\u9178\u9e7d(Phosphate)\u662f\u751f\u7269\u9ad4\u7cfb\u4e2d\u6700\u91cd\u8981\u7684\u7121\u6a5f\u96e2\u5b50(inorganic ions)\u4e4b\u4e00\u3002\u5b83\u5728\u5404\u7a2e\u89d2\u8272\u4e2d\u626e\u6f14\u91cd\u8981\u4f5c\u7528\u3002\u6700\u91cd\u8981\u7684\u4f5c\u7528\u4e4b\u4e00\u662f\u4f5c\u70ba\u5206\u5b50\u958b\u95dc(\u00a0molecular switch)\uff0c\u901a\u904e\u751f\u7269\u7cfb\u7d71\u4e2d\u5404\u7a2e\u86cb\u767d\u6fc0\u9176(protein kinases)\u548c\u78f7\u9178\u9176(phosphatases)\u7684\u4ecb\u5c0e\u4f7f\u9176\u6d3b\u6027(enzyme activity)\u958b\u555f\u548c\u95dc\u9589\u3002\u78f7\u9178\u9e7d\u53c3\u8207\u78f7\u9178\u9176\u3001ATP \u9176\u548c\u5176\u4ed6\u5e7e\u7a2e\u9176\u50ac\u5316\u5f9e\u5e95\u7269\u4e2d\u91cb\u653e\u7121\u6a5f\u78f7\u9178\u9e7d (Pi) \u7684\u53cd\u61c9\u3002\u78f7\u9178\u9e7d\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2( Phosphate Colorimetric Assay Kit, KA6676) \u63d0\u4f9b\u4e86\u4e00\u500b\u7c21\u55ae\uff0c\u5feb\u901f\u548c\u9748\u654f\u7684\u65b9\u6cd5\u4f86\u8a55\u4f30\u5404\u7a2e\u6fc3\u5ea6\u7684\u78f7\u9178\u9e7d(Phosphate)\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u5b54\u96c0\u77f3\u7da0( malachite green)\u548c\u926c\u9178\u92a8(ammonium molybdate)\u7684\u5c08\u6709\u914d\u65b9\uff0c\u5176\u8207\u78f7\u9178\u6839\u96e2\u5b50\u5f62\u6210\u986f\u8272\u8907\u5408\u7269( chromogenic complex )\uff0c\u5728600-660nm\u9644\u8fd1\u7522\u751f\u5f37\u70c8\u7684\u5438\u6536\u5e36\u3002\u8207\u5176\u4ed6\u5b54\u96c0\u77f3\u4e0d\u540c\u67d3\u6599\u914d\u65b9\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u63d0\u4f9b\u5b8c\u5168\u7a69\u5b9a\u7684\u7d42\u9ede\u4fe1\u865f\uff0c\u4e0d\u6613\u767c\u751f\u6c89\u6fb1\u3002\u53ef\u4ee5\u76f4\u63a5\u78ba\u5b9a\u78f7\u9178\u9e7d\u6fc3\u5ea6\u57280.78 &#8211; 50 uM\u4e4b\u9593\u3002Sample Volume:80 uL (96well\u5e73\u5e95\u900f\u660e\u76e4; 384well\u4f7f\u752840ul\u9ad4\u7a4d)<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/%E7%A3%B7%E9%85%B8%E9%B9%BD%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-Phosphate-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA6676.png\" width=\"602\" height=\"734\" \/><\/p>\n<div id=\"10000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Product Description:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Phosphate Assay Kit (Colorimetric) has been developed for measuring the activity of any inorganic phosphate(Pi)-generating enzyme.<\/li>\n<\/ul>\n<\/div>\n<div id=\"10200\">\n<ul>\n<li class=\"black12 sub_title\"><b>Sample Volume:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">80 uL<\/li>\n<\/ul>\n<\/div>\n<div id=\"10350\">\n<ul>\n<li class=\"black12 sub_title\"><b>Absorbance (nm):<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">600 &#8211; 660 nm<\/li>\n<\/ul>\n<\/div>\n<div id=\"10400\">\n<ul>\n<li class=\"black12 sub_title\"><b>Detection Method:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Colorimetric<\/li>\n<\/ul>\n<\/div>\n<div id=\"20000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Calibration Range:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">0.78 &#8211; 50 uM<\/li>\n<\/ul>\n<\/div>\n<div id=\"260100\">\n<ul>\n<li class=\"black12 sub_title\"><b>Regulatory Status:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">For research use only (RUO)<\/li>\n<\/ul>\n<\/div>\n<div id=\"290000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Quality Control Testing:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Standard curve<br \/>\n<b><\/b><br \/>\nThe standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.<\/li>\n<\/ul>\n<\/div>\n<div id=\"400000\">\n<ul>\n<li class=\"black12 sub_title\"><b>Storage Instruction:<\/b><\/li>\n<li class=\"black11_a_underline sub_content\">Store the kit at 4\u00b0C.<\/li>\n<\/ul>\n<\/div>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/1-\u78f7\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2Phosphate-Assay-Kit-\u8ca8\u865fAbnova\u2122-KA6676.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u78f7\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2| Phosphate Assay Kit\u00a0 \u8ca8\u865fAbnova\u2122 KA6676 \u78f7\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6073],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44442"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44442"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44442\/revisions"}],"predecessor-version":[{"id":45453,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44442\/revisions\/45453"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44442"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44442"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44442"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}